In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions

Autores
Salinas Palmieri, Maria Cecilia; Cavagnaro, Pablo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Garlic blue mold disease, caused by Penicillium allii, is responsible for important economic losses produced yearly in garlic field crops and in postharvest storage. The identification of genetic resistance sources may help in the management of this disease. The present study investigated in vivo and in vitro antifungal effects of a genetically-diverse garlic collection against P. allii in two growing seasons. Cloves of garlic accessions were inoculated with P. allii conidia, and lesion area and sporulation of the fungus in the clove were estimated as a means to characterize the accessions response to the infection. Significant and continuous variation was found among the accessions (p < 0.001), suggesting a quantitative mode of resistance against P. allii in this garlic collection. ‘Castaño’ and ‘Peteco’ were consistently the most resistant and susceptible genotypes, respectively. Culture media of the fungus supplemented with crude garlic extracts (CGEs) revealed significant inhibition of P. allii growth in vitro by all the garlic accessions, with fungal-growth inhibition being dose-dependent up to a concentration of 20–26% (v/v), beyond which the CGEs became fungicidal. These data indicate that garlic bulbs have antifungal compounds, which at high concentration can be fungicidal for P. allii. CGEs of ‘Castaño’ and a few other accessions were consistently the more inhibitory extracts. Total phenolics and pyruvate (an estimator of the total thiosulfinates content) concentration in the garlic bulbs was not associated with P. allii resistance in vivo, but their content in the CGEs correlated significantly and negatively with the fungal growth in vitro (r = −0.38 to −0.72), suggesting that these compounds have antifungal effect, but their content is not the main factor conditioning P. allii resistance in vivo.
EEA La Consulta
Fil: Salinas Palmieri, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fuente
European Journal of Plant Pathology : 1-15 (First Online: 14 November 2019)
Materia
Ajo
Experimentación in Vitro
Experimentación in Vivo
Penicillium
Enfermedades de las Plantas
Control de Enfermedades
Resistencia a la Enfermedad
Garlic
In Vitro Experimentation
In Vivo Experimentation
Plant Diseases
Disease Control
Disease Resistance
Penicillium allii
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6396
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6396
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network_name_str INTA Digital (INTA)
spelling In vivo and in vitro screening for resistance against Penicillium allii in garlic accessionsSalinas Palmieri, Maria CeciliaCavagnaro, PabloAjoExperimentación in VitroExperimentación in VivoPenicilliumEnfermedades de las PlantasControl de EnfermedadesResistencia a la EnfermedadGarlicIn Vitro ExperimentationIn Vivo ExperimentationPlant DiseasesDisease ControlDisease ResistancePenicillium alliiGarlic blue mold disease, caused by Penicillium allii, is responsible for important economic losses produced yearly in garlic field crops and in postharvest storage. The identification of genetic resistance sources may help in the management of this disease. The present study investigated in vivo and in vitro antifungal effects of a genetically-diverse garlic collection against P. allii in two growing seasons. Cloves of garlic accessions were inoculated with P. allii conidia, and lesion area and sporulation of the fungus in the clove were estimated as a means to characterize the accessions response to the infection. Significant and continuous variation was found among the accessions (p < 0.001), suggesting a quantitative mode of resistance against P. allii in this garlic collection. ‘Castaño’ and ‘Peteco’ were consistently the most resistant and susceptible genotypes, respectively. Culture media of the fungus supplemented with crude garlic extracts (CGEs) revealed significant inhibition of P. allii growth in vitro by all the garlic accessions, with fungal-growth inhibition being dose-dependent up to a concentration of 20–26% (v/v), beyond which the CGEs became fungicidal. These data indicate that garlic bulbs have antifungal compounds, which at high concentration can be fungicidal for P. allii. CGEs of ‘Castaño’ and a few other accessions were consistently the more inhibitory extracts. Total phenolics and pyruvate (an estimator of the total thiosulfinates content) concentration in the garlic bulbs was not associated with P. allii resistance in vivo, but their content in the CGEs correlated significantly and negatively with the fungal growth in vitro (r = −0.38 to −0.72), suggesting that these compounds have antifungal effect, but their content is not the main factor conditioning P. allii resistance in vivo.EEA La ConsultaFil: Salinas Palmieri, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaSpringer2019-11-26T12:32:41Z2019-11-26T12:32:41Z2019-11-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s10658-019-01875-zhttp://hdl.handle.net/20.500.12123/63960929-18731573-8469https://doi.org/10.1007/s10658-019-01875-zEuropean Journal of Plant Pathology : 1-15 (First Online: 14 November 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:16Zoai:localhost:20.500.12123/6396instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:17.398INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions
title In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions
spellingShingle In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions
Salinas Palmieri, Maria Cecilia
Ajo
Experimentación in Vitro
Experimentación in Vivo
Penicillium
Enfermedades de las Plantas
Control de Enfermedades
Resistencia a la Enfermedad
Garlic
In Vitro Experimentation
In Vivo Experimentation
Plant Diseases
Disease Control
Disease Resistance
Penicillium allii
title_short In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions
title_full In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions
title_fullStr In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions
title_full_unstemmed In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions
title_sort In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions
dc.creator.none.fl_str_mv Salinas Palmieri, Maria Cecilia
Cavagnaro, Pablo
author Salinas Palmieri, Maria Cecilia
author_facet Salinas Palmieri, Maria Cecilia
Cavagnaro, Pablo
author_role author
author2 Cavagnaro, Pablo
author2_role author
dc.subject.none.fl_str_mv Ajo
Experimentación in Vitro
Experimentación in Vivo
Penicillium
Enfermedades de las Plantas
Control de Enfermedades
Resistencia a la Enfermedad
Garlic
In Vitro Experimentation
In Vivo Experimentation
Plant Diseases
Disease Control
Disease Resistance
Penicillium allii
topic Ajo
Experimentación in Vitro
Experimentación in Vivo
Penicillium
Enfermedades de las Plantas
Control de Enfermedades
Resistencia a la Enfermedad
Garlic
In Vitro Experimentation
In Vivo Experimentation
Plant Diseases
Disease Control
Disease Resistance
Penicillium allii
dc.description.none.fl_txt_mv Garlic blue mold disease, caused by Penicillium allii, is responsible for important economic losses produced yearly in garlic field crops and in postharvest storage. The identification of genetic resistance sources may help in the management of this disease. The present study investigated in vivo and in vitro antifungal effects of a genetically-diverse garlic collection against P. allii in two growing seasons. Cloves of garlic accessions were inoculated with P. allii conidia, and lesion area and sporulation of the fungus in the clove were estimated as a means to characterize the accessions response to the infection. Significant and continuous variation was found among the accessions (p < 0.001), suggesting a quantitative mode of resistance against P. allii in this garlic collection. ‘Castaño’ and ‘Peteco’ were consistently the most resistant and susceptible genotypes, respectively. Culture media of the fungus supplemented with crude garlic extracts (CGEs) revealed significant inhibition of P. allii growth in vitro by all the garlic accessions, with fungal-growth inhibition being dose-dependent up to a concentration of 20–26% (v/v), beyond which the CGEs became fungicidal. These data indicate that garlic bulbs have antifungal compounds, which at high concentration can be fungicidal for P. allii. CGEs of ‘Castaño’ and a few other accessions were consistently the more inhibitory extracts. Total phenolics and pyruvate (an estimator of the total thiosulfinates content) concentration in the garlic bulbs was not associated with P. allii resistance in vivo, but their content in the CGEs correlated significantly and negatively with the fungal growth in vitro (r = −0.38 to −0.72), suggesting that these compounds have antifungal effect, but their content is not the main factor conditioning P. allii resistance in vivo.
EEA La Consulta
Fil: Salinas Palmieri, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
description Garlic blue mold disease, caused by Penicillium allii, is responsible for important economic losses produced yearly in garlic field crops and in postharvest storage. The identification of genetic resistance sources may help in the management of this disease. The present study investigated in vivo and in vitro antifungal effects of a genetically-diverse garlic collection against P. allii in two growing seasons. Cloves of garlic accessions were inoculated with P. allii conidia, and lesion area and sporulation of the fungus in the clove were estimated as a means to characterize the accessions response to the infection. Significant and continuous variation was found among the accessions (p < 0.001), suggesting a quantitative mode of resistance against P. allii in this garlic collection. ‘Castaño’ and ‘Peteco’ were consistently the most resistant and susceptible genotypes, respectively. Culture media of the fungus supplemented with crude garlic extracts (CGEs) revealed significant inhibition of P. allii growth in vitro by all the garlic accessions, with fungal-growth inhibition being dose-dependent up to a concentration of 20–26% (v/v), beyond which the CGEs became fungicidal. These data indicate that garlic bulbs have antifungal compounds, which at high concentration can be fungicidal for P. allii. CGEs of ‘Castaño’ and a few other accessions were consistently the more inhibitory extracts. Total phenolics and pyruvate (an estimator of the total thiosulfinates content) concentration in the garlic bulbs was not associated with P. allii resistance in vivo, but their content in the CGEs correlated significantly and negatively with the fungal growth in vitro (r = −0.38 to −0.72), suggesting that these compounds have antifungal effect, but their content is not the main factor conditioning P. allii resistance in vivo.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-26T12:32:41Z
2019-11-26T12:32:41Z
2019-11-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s10658-019-01875-z
http://hdl.handle.net/20.500.12123/6396
0929-1873
1573-8469
https://doi.org/10.1007/s10658-019-01875-z
url https://link.springer.com/article/10.1007/s10658-019-01875-z
http://hdl.handle.net/20.500.12123/6396
https://doi.org/10.1007/s10658-019-01875-z
identifier_str_mv 0929-1873
1573-8469
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv European Journal of Plant Pathology : 1-15 (First Online: 14 November 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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