Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.)

Autores
Carloni, Edgardo José; Rodriguez, Milena Nahir; Tommasino, Exequiel Arturo; Ruolo, Maria Soledad; Grunberg, Karina
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hops (Humulus lupulus L.) is a dioecious plant of the Cannabaceae family, highly valued in beer production. In vitro culture has been established as a tool in agricultural biotechnology for its propagation and improvement. This study evaluated the impact of different concentrations of cytokinins, to develop an efficient protocol for in vitro micropropagation of the species. Concentrations of 0.1, 1, 3, and 6 mg·L−1 of BAP and KIN and control without cytokinins were used. After 28 days of culture, growth and development variables were analysed. The results indicated that lower concentrations of cytokinins favoured most of the variables studied. In contrast, concentrations equal to or higher than 1 mg·L−1 of BAP and KIN reduced growth, and increased callus formation and hyperhydricity. The protocol developed proved efficient, with more than 90% of the explants generating at least two shoots and approximately the same percentage developing roots. In addition, seedlings obtained with the 0.1 mg·L−1 KIN and BAP treatment showed high survival after transplanting. Applying low concentrations of cytokinins eliminated the need for an additional rooting stage, optimising the micropropagation process
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
Fil: Rodriguez, Milena Nahir. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Milena Nahir.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
Fil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Ruolo, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Ruolo, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
Fil: Ruolo, Maria Soledad. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Grunberg, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
Fuente
New Zealand Journal of Crop and Horticultural Science (Published online: 05 Feb 2025)
Materia
6-benzylaminopurine
Kinetin
Callus
Hops
6-benziladenina
Quinetina
Callo
Lúpulo
Humulus lupulus
Micropropagation Protocol
Lupulin
Hyperhydricity
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21483
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21483
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.)Carloni, Edgardo JoséRodriguez, Milena NahirTommasino, Exequiel ArturoRuolo, Maria SoledadGrunberg, Karina6-benzylaminopurineKinetinCallusHops6-benziladeninaQuinetinaCalloLúpuloHumulus lupulusMicropropagation ProtocolLupulinHyperhydricityHops (Humulus lupulus L.) is a dioecious plant of the Cannabaceae family, highly valued in beer production. In vitro culture has been established as a tool in agricultural biotechnology for its propagation and improvement. This study evaluated the impact of different concentrations of cytokinins, to develop an efficient protocol for in vitro micropropagation of the species. Concentrations of 0.1, 1, 3, and 6 mg·L−1 of BAP and KIN and control without cytokinins were used. After 28 days of culture, growth and development variables were analysed. The results indicated that lower concentrations of cytokinins favoured most of the variables studied. In contrast, concentrations equal to or higher than 1 mg·L−1 of BAP and KIN reduced growth, and increased callus formation and hyperhydricity. The protocol developed proved efficient, with more than 90% of the explants generating at least two shoots and approximately the same percentage developing roots. In addition, seedlings obtained with the 0.1 mg·L−1 KIN and BAP treatment showed high survival after transplanting. Applying low concentrations of cytokinins eliminated the need for an additional rooting stage, optimising the micropropagation processInstituto de Fisiología y Recursos Genéticos VegetalesFil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; ArgentinaFil: Rodriguez, Milena Nahir. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Rodriguez, Milena Nahir.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; ArgentinaFil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Ruolo, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Ruolo, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; ArgentinaFil: Ruolo, Maria Soledad. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Grunberg, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; ArgentinaTaylor and Francis2025-02-27T10:29:54Z2025-02-27T10:29:54Z2025-01-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21483https://www.tandfonline.com/doi/10.1080/01140671.2025.24588120114-06711175-8783https://doi.org/10.1080/01140671.2025.2458812New Zealand Journal of Crop and Horticultural Science (Published online: 05 Feb 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-RIST-E4-I091-001, Multiplicación de propágulos de sanidad controladainfo:eu-repograntAgreement/INTA/2023-PD-L01-I071, Mejoramiento genético de los Cultivos Industriales y estrategias alternativas para la obtención de biotipos adaptados a los nuevos escenarios y al cambio climáticoinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:56Zoai:localhost:20.500.12123/21483instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:57.309INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.)
title Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.)
spellingShingle Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.)
Carloni, Edgardo José
6-benzylaminopurine
Kinetin
Callus
Hops
6-benziladenina
Quinetina
Callo
Lúpulo
Humulus lupulus
Micropropagation Protocol
Lupulin
Hyperhydricity
title_short Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.)
title_full Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.)
title_fullStr Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.)
title_full_unstemmed Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.)
title_sort Effect of different cytokinin concentrations on establishing an in vitro micropropagation system for hop (Humulus lupulus L.)
dc.creator.none.fl_str_mv Carloni, Edgardo José
Rodriguez, Milena Nahir
Tommasino, Exequiel Arturo
Ruolo, Maria Soledad
Grunberg, Karina
author Carloni, Edgardo José
author_facet Carloni, Edgardo José
Rodriguez, Milena Nahir
Tommasino, Exequiel Arturo
Ruolo, Maria Soledad
Grunberg, Karina
author_role author
author2 Rodriguez, Milena Nahir
Tommasino, Exequiel Arturo
Ruolo, Maria Soledad
Grunberg, Karina
author2_role author
author
author
author
dc.subject.none.fl_str_mv 6-benzylaminopurine
Kinetin
Callus
Hops
6-benziladenina
Quinetina
Callo
Lúpulo
Humulus lupulus
Micropropagation Protocol
Lupulin
Hyperhydricity
topic 6-benzylaminopurine
Kinetin
Callus
Hops
6-benziladenina
Quinetina
Callo
Lúpulo
Humulus lupulus
Micropropagation Protocol
Lupulin
Hyperhydricity
dc.description.none.fl_txt_mv Hops (Humulus lupulus L.) is a dioecious plant of the Cannabaceae family, highly valued in beer production. In vitro culture has been established as a tool in agricultural biotechnology for its propagation and improvement. This study evaluated the impact of different concentrations of cytokinins, to develop an efficient protocol for in vitro micropropagation of the species. Concentrations of 0.1, 1, 3, and 6 mg·L−1 of BAP and KIN and control without cytokinins were used. After 28 days of culture, growth and development variables were analysed. The results indicated that lower concentrations of cytokinins favoured most of the variables studied. In contrast, concentrations equal to or higher than 1 mg·L−1 of BAP and KIN reduced growth, and increased callus formation and hyperhydricity. The protocol developed proved efficient, with more than 90% of the explants generating at least two shoots and approximately the same percentage developing roots. In addition, seedlings obtained with the 0.1 mg·L−1 KIN and BAP treatment showed high survival after transplanting. Applying low concentrations of cytokinins eliminated the need for an additional rooting stage, optimising the micropropagation process
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
Fil: Rodriguez, Milena Nahir. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Milena Nahir.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
Fil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Ruolo, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Ruolo, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
Fil: Ruolo, Maria Soledad. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Grunberg, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina
description Hops (Humulus lupulus L.) is a dioecious plant of the Cannabaceae family, highly valued in beer production. In vitro culture has been established as a tool in agricultural biotechnology for its propagation and improvement. This study evaluated the impact of different concentrations of cytokinins, to develop an efficient protocol for in vitro micropropagation of the species. Concentrations of 0.1, 1, 3, and 6 mg·L−1 of BAP and KIN and control without cytokinins were used. After 28 days of culture, growth and development variables were analysed. The results indicated that lower concentrations of cytokinins favoured most of the variables studied. In contrast, concentrations equal to or higher than 1 mg·L−1 of BAP and KIN reduced growth, and increased callus formation and hyperhydricity. The protocol developed proved efficient, with more than 90% of the explants generating at least two shoots and approximately the same percentage developing roots. In addition, seedlings obtained with the 0.1 mg·L−1 KIN and BAP treatment showed high survival after transplanting. Applying low concentrations of cytokinins eliminated the need for an additional rooting stage, optimising the micropropagation process
publishDate 2025
dc.date.none.fl_str_mv 2025-02-27T10:29:54Z
2025-02-27T10:29:54Z
2025-01-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21483
https://www.tandfonline.com/doi/10.1080/01140671.2025.2458812
0114-0671
1175-8783
https://doi.org/10.1080/01140671.2025.2458812
url http://hdl.handle.net/20.500.12123/21483
https://www.tandfonline.com/doi/10.1080/01140671.2025.2458812
https://doi.org/10.1080/01140671.2025.2458812
identifier_str_mv 0114-0671
1175-8783
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-RIST-E4-I091-001, Multiplicación de propágulos de sanidad controlada
info:eu-repograntAgreement/INTA/2023-PD-L01-I071, Mejoramiento genético de los Cultivos Industriales y estrategias alternativas para la obtención de biotipos adaptados a los nuevos escenarios y al cambio climático
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv New Zealand Journal of Crop and Horticultural Science (Published online: 05 Feb 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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