Detection of viral and viroid pathogens co-infecting Humulus lupulus in Argentina

Autores
Nome Docampo, Claudia; Tommasino, Exequiel Arturo; Carloni, Edgardo José; Ruolo, Maria Soledad; De Breuil, Soledad
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hops (Humulus lupulus L.) are primarily cultivated for their cones, which contain bioactive compounds essential to the brewing and pharmaceutical industries. Its vegetative propagation has facilitated the storage and spread of pathogens, including viruses and viroids, negatively impacting crop productivity. In this work we study symptomatic hop plants cultivated in Argentina, employing transmission electron microscopy, RT-PCR, and high-throughput sequencing combined with phylogenetic analysis to diagnose and identify the causal agents. The analysis confirmed, for the first time in Argentina, the presence of hop mosaic virus, hop latent virus, and hop latent viroid in various commercial hop cultivars. These findings indicate the urgent need for integrated disease management strategies, including the use of virus-free propagation material and the implementation of early detection protocols for viruses and viroids in the field. Such measures are vital for maintaining sustainable hop production and meeting the demands of Argentina’s growing brewing industry.
Instituto de Patología Vegetal
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Ruolo, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Ruolo, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Ruolo, Maria Soledad. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: De Breuil, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
Journal of plant pathology : 1-7 (Published: 12 February 2026)
Materia
Electron Microscopy
Humulus lupulus
Hops
Microscopía Electrónica
Argentina
Lúpulo
Hop Mosaic Virus
Hop Latent Virus
Hop Latent Viroid
Molecular Analysis
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25290
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oai_identifier_str oai:localhost:20.500.12123/25290
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Detection of viral and viroid pathogens co-infecting Humulus lupulus in ArgentinaNome Docampo, ClaudiaTommasino, Exequiel ArturoCarloni, Edgardo JoséRuolo, Maria SoledadDe Breuil, SoledadElectron MicroscopyHumulus lupulusHopsMicroscopía ElectrónicaArgentinaLúpuloHop Mosaic VirusHop Latent VirusHop Latent ViroidMolecular AnalysisHops (Humulus lupulus L.) are primarily cultivated for their cones, which contain bioactive compounds essential to the brewing and pharmaceutical industries. Its vegetative propagation has facilitated the storage and spread of pathogens, including viruses and viroids, negatively impacting crop productivity. In this work we study symptomatic hop plants cultivated in Argentina, employing transmission electron microscopy, RT-PCR, and high-throughput sequencing combined with phylogenetic analysis to diagnose and identify the causal agents. The analysis confirmed, for the first time in Argentina, the presence of hop mosaic virus, hop latent virus, and hop latent viroid in various commercial hop cultivars. These findings indicate the urgent need for integrated disease management strategies, including the use of virus-free propagation material and the implementation of early detection protocols for viruses and viroids in the field. Such measures are vital for maintaining sustainable hop production and meeting the demands of Argentina’s growing brewing industry.Instituto de Patología VegetalFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Nome Docampo, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Ruolo, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Ruolo, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Ruolo, Maria Soledad. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: De Breuil, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaSpringer2026-02-25T11:26:08Z2026-02-25T11:26:08Z2026-02-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25290https://link.springer.com/article/10.1007/s42161-026-02143-w1125-46532239-7264 (online)https://doi.org/10.1007/s42161-026-02143-wJournal of plant pathology : 1-7 (Published: 12 February 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejoinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:43Zoai:localhost:20.500.12123/25290instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:43.225INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection of viral and viroid pathogens co-infecting Humulus lupulus in Argentina
title Detection of viral and viroid pathogens co-infecting Humulus lupulus in Argentina
spellingShingle Detection of viral and viroid pathogens co-infecting Humulus lupulus in Argentina
Nome Docampo, Claudia
Electron Microscopy
Humulus lupulus
Hops
Microscopía Electrónica
Argentina
Lúpulo
Hop Mosaic Virus
Hop Latent Virus
Hop Latent Viroid
Molecular Analysis
title_short Detection of viral and viroid pathogens co-infecting Humulus lupulus in Argentina
title_full Detection of viral and viroid pathogens co-infecting Humulus lupulus in Argentina
title_fullStr Detection of viral and viroid pathogens co-infecting Humulus lupulus in Argentina
title_full_unstemmed Detection of viral and viroid pathogens co-infecting Humulus lupulus in Argentina
title_sort Detection of viral and viroid pathogens co-infecting Humulus lupulus in Argentina
dc.creator.none.fl_str_mv Nome Docampo, Claudia
Tommasino, Exequiel Arturo
Carloni, Edgardo José
Ruolo, Maria Soledad
De Breuil, Soledad
author Nome Docampo, Claudia
author_facet Nome Docampo, Claudia
Tommasino, Exequiel Arturo
Carloni, Edgardo José
Ruolo, Maria Soledad
De Breuil, Soledad
author_role author
author2 Tommasino, Exequiel Arturo
Carloni, Edgardo José
Ruolo, Maria Soledad
De Breuil, Soledad
author2_role author
author
author
author
dc.subject.none.fl_str_mv Electron Microscopy
Humulus lupulus
Hops
Microscopía Electrónica
Argentina
Lúpulo
Hop Mosaic Virus
Hop Latent Virus
Hop Latent Viroid
Molecular Analysis
topic Electron Microscopy
Humulus lupulus
Hops
Microscopía Electrónica
Argentina
Lúpulo
Hop Mosaic Virus
Hop Latent Virus
Hop Latent Viroid
Molecular Analysis
dc.description.none.fl_txt_mv Hops (Humulus lupulus L.) are primarily cultivated for their cones, which contain bioactive compounds essential to the brewing and pharmaceutical industries. Its vegetative propagation has facilitated the storage and spread of pathogens, including viruses and viroids, negatively impacting crop productivity. In this work we study symptomatic hop plants cultivated in Argentina, employing transmission electron microscopy, RT-PCR, and high-throughput sequencing combined with phylogenetic analysis to diagnose and identify the causal agents. The analysis confirmed, for the first time in Argentina, the presence of hop mosaic virus, hop latent virus, and hop latent viroid in various commercial hop cultivars. These findings indicate the urgent need for integrated disease management strategies, including the use of virus-free propagation material and the implementation of early detection protocols for viruses and viroids in the field. Such measures are vital for maintaining sustainable hop production and meeting the demands of Argentina’s growing brewing industry.
Instituto de Patología Vegetal
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Ruolo, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Ruolo, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Ruolo, Maria Soledad. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: De Breuil, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description Hops (Humulus lupulus L.) are primarily cultivated for their cones, which contain bioactive compounds essential to the brewing and pharmaceutical industries. Its vegetative propagation has facilitated the storage and spread of pathogens, including viruses and viroids, negatively impacting crop productivity. In this work we study symptomatic hop plants cultivated in Argentina, employing transmission electron microscopy, RT-PCR, and high-throughput sequencing combined with phylogenetic analysis to diagnose and identify the causal agents. The analysis confirmed, for the first time in Argentina, the presence of hop mosaic virus, hop latent virus, and hop latent viroid in various commercial hop cultivars. These findings indicate the urgent need for integrated disease management strategies, including the use of virus-free propagation material and the implementation of early detection protocols for viruses and viroids in the field. Such measures are vital for maintaining sustainable hop production and meeting the demands of Argentina’s growing brewing industry.
publishDate 2026
dc.date.none.fl_str_mv 2026-02-25T11:26:08Z
2026-02-25T11:26:08Z
2026-02-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25290
https://link.springer.com/article/10.1007/s42161-026-02143-w
1125-4653
2239-7264 (online)
https://doi.org/10.1007/s42161-026-02143-w
url http://hdl.handle.net/20.500.12123/25290
https://link.springer.com/article/10.1007/s42161-026-02143-w
https://doi.org/10.1007/s42161-026-02143-w
identifier_str_mv 1125-4653
2239-7264 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Journal of plant pathology : 1-7 (Published: 12 February 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.176822

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