Nanostructured voltammetric sensor for ultra-trace anabolic drug determination in food safety field

Autores
Regiart, Daniel Matias Gaston; Pereira, Sirley Vanesa; Spotorno, Viviana Graciela; Bertolino, Franco Adrián; Raba, Julio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A sensitive electrochemical sensor for clenbuterol (CL) determination in bovine urine samples was designed. This drug was originally developed for the treatment of respiratory pathologies. However, in the livestock industry, CL has been used as growth promoter because it stimulates the muscle growth and lipid degradation. The drug residues accumulated in animal tissues represent a potential risk for the human health. Subsequently, the development of a fast and simple method for the CL quantification employed to detect its abuse in livestock production is an attractive analytical challenge. The proposed method was based on the use of a screen printed carbon electrode (SPCE) modified with multiwall carbon nanotubes (MWCNTs) in a matrix of chitosan (CH) on gold nanoparticles (AuNPs) previously incorporated by electrodeposition process. The MWCNTs-CH/AuNPs/SPCE was characterized by cyclic voltammetry, X-ray diffraction and scan electron microscopy. With the aim to carry out the CL determination, a pre-concentration procedure was required. For this purpose, we employed anti-CL antibodies immobilized on magnetic microparticles (MPs) as bioaffinity support to capture the drug present in the sample. This support was exposed to a desorption process. Later, the electrochemical detection by square wave voltammetry (OSWV) was performed. The sensor exhibited excellent performances with a linear response range in concentrations from 0.01 to 6 ng mL−1. The detection and quantification limits obtained were 0.003 and 0.01 ng mL−1, respectively with an analysis time of 19 min and a R.S.D. below 5.8%. As a conclusion, we can claim that the results obtained by the proposed sensor were successful.
Instituto de Recursos Biológicos
Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Spotorno, Viviana Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fuente
Sensors and Actuators B: Chemical 188 : 1241-1249 (November 2013)
Materia
Sensores
Clenbuterol
Medicamentos Neurotrópicos
Ganado
Nanotecnología
Inocuidad Alimentaria
Sensors
Neurotropic Drugs
Livestock
Nanotechnology
Food Safety
Anabolics
Anabólicos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3344
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3344
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Nanostructured voltammetric sensor for ultra-trace anabolic drug determination in food safety fieldRegiart, Daniel Matias GastonPereira, Sirley VanesaSpotorno, Viviana GracielaBertolino, Franco AdriánRaba, JulioSensoresClenbuterolMedicamentos NeurotrópicosGanadoNanotecnologíaInocuidad AlimentariaSensorsNeurotropic DrugsLivestockNanotechnologyFood SafetyAnabolicsAnabólicosA sensitive electrochemical sensor for clenbuterol (CL) determination in bovine urine samples was designed. This drug was originally developed for the treatment of respiratory pathologies. However, in the livestock industry, CL has been used as growth promoter because it stimulates the muscle growth and lipid degradation. The drug residues accumulated in animal tissues represent a potential risk for the human health. Subsequently, the development of a fast and simple method for the CL quantification employed to detect its abuse in livestock production is an attractive analytical challenge. The proposed method was based on the use of a screen printed carbon electrode (SPCE) modified with multiwall carbon nanotubes (MWCNTs) in a matrix of chitosan (CH) on gold nanoparticles (AuNPs) previously incorporated by electrodeposition process. The MWCNTs-CH/AuNPs/SPCE was characterized by cyclic voltammetry, X-ray diffraction and scan electron microscopy. With the aim to carry out the CL determination, a pre-concentration procedure was required. For this purpose, we employed anti-CL antibodies immobilized on magnetic microparticles (MPs) as bioaffinity support to capture the drug present in the sample. This support was exposed to a desorption process. Later, the electrochemical detection by square wave voltammetry (OSWV) was performed. The sensor exhibited excellent performances with a linear response range in concentrations from 0.01 to 6 ng mL−1. The detection and quantification limits obtained were 0.003 and 0.01 ng mL−1, respectively with an analysis time of 19 min and a R.S.D. below 5.8%. As a conclusion, we can claim that the results obtained by the proposed sensor were successful.Instituto de Recursos BiológicosFil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; ArgentinaFil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; ArgentinaFil: Spotorno, Viviana Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina2018-09-12T15:29:58Z2018-09-12T15:29:58Z2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S092540051300943Xhttp://hdl.handle.net/20.500.12123/33440925-4005https://doi.org/10.1016/j.snb.2013.07.117Sensors and Actuators B: Chemical 188 : 1241-1249 (November 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:39Zoai:localhost:20.500.12123/3344instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:39.954INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Nanostructured voltammetric sensor for ultra-trace anabolic drug determination in food safety field
title Nanostructured voltammetric sensor for ultra-trace anabolic drug determination in food safety field
spellingShingle Nanostructured voltammetric sensor for ultra-trace anabolic drug determination in food safety field
Regiart, Daniel Matias Gaston
Sensores
Clenbuterol
Medicamentos Neurotrópicos
Ganado
Nanotecnología
Inocuidad Alimentaria
Sensors
Neurotropic Drugs
Livestock
Nanotechnology
Food Safety
Anabolics
Anabólicos
title_short Nanostructured voltammetric sensor for ultra-trace anabolic drug determination in food safety field
title_full Nanostructured voltammetric sensor for ultra-trace anabolic drug determination in food safety field
title_fullStr Nanostructured voltammetric sensor for ultra-trace anabolic drug determination in food safety field
title_full_unstemmed Nanostructured voltammetric sensor for ultra-trace anabolic drug determination in food safety field
title_sort Nanostructured voltammetric sensor for ultra-trace anabolic drug determination in food safety field
dc.creator.none.fl_str_mv Regiart, Daniel Matias Gaston
Pereira, Sirley Vanesa
Spotorno, Viviana Graciela
Bertolino, Franco Adrián
Raba, Julio
author Regiart, Daniel Matias Gaston
author_facet Regiart, Daniel Matias Gaston
Pereira, Sirley Vanesa
Spotorno, Viviana Graciela
Bertolino, Franco Adrián
Raba, Julio
author_role author
author2 Pereira, Sirley Vanesa
Spotorno, Viviana Graciela
Bertolino, Franco Adrián
Raba, Julio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Sensores
Clenbuterol
Medicamentos Neurotrópicos
Ganado
Nanotecnología
Inocuidad Alimentaria
Sensors
Neurotropic Drugs
Livestock
Nanotechnology
Food Safety
Anabolics
Anabólicos
topic Sensores
Clenbuterol
Medicamentos Neurotrópicos
Ganado
Nanotecnología
Inocuidad Alimentaria
Sensors
Neurotropic Drugs
Livestock
Nanotechnology
Food Safety
Anabolics
Anabólicos
dc.description.none.fl_txt_mv A sensitive electrochemical sensor for clenbuterol (CL) determination in bovine urine samples was designed. This drug was originally developed for the treatment of respiratory pathologies. However, in the livestock industry, CL has been used as growth promoter because it stimulates the muscle growth and lipid degradation. The drug residues accumulated in animal tissues represent a potential risk for the human health. Subsequently, the development of a fast and simple method for the CL quantification employed to detect its abuse in livestock production is an attractive analytical challenge. The proposed method was based on the use of a screen printed carbon electrode (SPCE) modified with multiwall carbon nanotubes (MWCNTs) in a matrix of chitosan (CH) on gold nanoparticles (AuNPs) previously incorporated by electrodeposition process. The MWCNTs-CH/AuNPs/SPCE was characterized by cyclic voltammetry, X-ray diffraction and scan electron microscopy. With the aim to carry out the CL determination, a pre-concentration procedure was required. For this purpose, we employed anti-CL antibodies immobilized on magnetic microparticles (MPs) as bioaffinity support to capture the drug present in the sample. This support was exposed to a desorption process. Later, the electrochemical detection by square wave voltammetry (OSWV) was performed. The sensor exhibited excellent performances with a linear response range in concentrations from 0.01 to 6 ng mL−1. The detection and quantification limits obtained were 0.003 and 0.01 ng mL−1, respectively with an analysis time of 19 min and a R.S.D. below 5.8%. As a conclusion, we can claim that the results obtained by the proposed sensor were successful.
Instituto de Recursos Biológicos
Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Spotorno, Viviana Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
description A sensitive electrochemical sensor for clenbuterol (CL) determination in bovine urine samples was designed. This drug was originally developed for the treatment of respiratory pathologies. However, in the livestock industry, CL has been used as growth promoter because it stimulates the muscle growth and lipid degradation. The drug residues accumulated in animal tissues represent a potential risk for the human health. Subsequently, the development of a fast and simple method for the CL quantification employed to detect its abuse in livestock production is an attractive analytical challenge. The proposed method was based on the use of a screen printed carbon electrode (SPCE) modified with multiwall carbon nanotubes (MWCNTs) in a matrix of chitosan (CH) on gold nanoparticles (AuNPs) previously incorporated by electrodeposition process. The MWCNTs-CH/AuNPs/SPCE was characterized by cyclic voltammetry, X-ray diffraction and scan electron microscopy. With the aim to carry out the CL determination, a pre-concentration procedure was required. For this purpose, we employed anti-CL antibodies immobilized on magnetic microparticles (MPs) as bioaffinity support to capture the drug present in the sample. This support was exposed to a desorption process. Later, the electrochemical detection by square wave voltammetry (OSWV) was performed. The sensor exhibited excellent performances with a linear response range in concentrations from 0.01 to 6 ng mL−1. The detection and quantification limits obtained were 0.003 and 0.01 ng mL−1, respectively with an analysis time of 19 min and a R.S.D. below 5.8%. As a conclusion, we can claim that the results obtained by the proposed sensor were successful.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
2018-09-12T15:29:58Z
2018-09-12T15:29:58Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S092540051300943X
http://hdl.handle.net/20.500.12123/3344
0925-4005
https://doi.org/10.1016/j.snb.2013.07.117
url https://www.sciencedirect.com/science/article/pii/S092540051300943X
http://hdl.handle.net/20.500.12123/3344
https://doi.org/10.1016/j.snb.2013.07.117
identifier_str_mv 0925-4005
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Sensors and Actuators B: Chemical 188 : 1241-1249 (November 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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