Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field

Autores
Bertolino, Franco Adrián; Pereira, Sirley Vanesa; Spotorno, Viviana G.; Raba, Julio; Regiart, Daniel Matias Gaston
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
tA sensitive electrochemical sensor for clenbuterol (CL) determination in bovine urine samples wasdesigned. This drug was originally developed for the treatment of respiratory pathologies. However,in the livestock industry, CL has been used as growth promoter because it stimulates the muscle growthand lipid degradation. The drug residues accumulated in animal tissues represent a potential risk forthe human health. Subsequently, the development of a fast and simple method for the CL quantificationemployed to detect its abuse in livestock production is an attractive analytical challenge.The proposed method was based on the use of a screen printed carbon electrode (SPCE) modifiedwith multiwall carbon nanotubes (MWCNTs) in a matrix of chitosan (CH) on gold nanoparticles (AuNPs)previously incorporated by electrodeposition process. The MWCNTs-CH/AuNPs/SPCE was characterizedby cyclic voltammetry, X-ray diffraction and scan electron microscopy.With the aim to carry out the CL determination, a pre-concentration procedure was required. For thispurpose, we employed anti-CL antibodies immobilized on magnetic microparticles (MPs) as bioaffinitysupport to capture the drug present in the sample. This support was exposed to a desorption process.Later, the electrochemical detection by square wave voltammetry (OSWV) was performed. The sensorexhibited excellent performances with a linear response range in concentrations from 0.01 to 6 ng mL−1.The detection and quantification limits obtained were 0.003 and 0.01 ng mL−1, respectively with an anal-ysis time of 19 min and a R.S.D. below 5.8%. As a conclusion, we can claim that the results obtained bythe proposed sensor were successful.
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Spotorno, Viviana G.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Materia
Voltametric Sensor
Nanostructured Platform
Anabolic Drug
Food Safety
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5709
Acceder
id CONICETDig_f9b40ad124d343e3da0ecaee4801606a
oai_identifier_str oai:ri.conicet.gov.ar:11336/5709
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety fieldBertolino, Franco AdriánPereira, Sirley VanesaSpotorno, Viviana G.Raba, JulioRegiart, Daniel Matias GastonVoltametric SensorNanostructured PlatformAnabolic DrugFood Safetyhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1tA sensitive electrochemical sensor for clenbuterol (CL) determination in bovine urine samples wasdesigned. This drug was originally developed for the treatment of respiratory pathologies. However,in the livestock industry, CL has been used as growth promoter because it stimulates the muscle growthand lipid degradation. The drug residues accumulated in animal tissues represent a potential risk forthe human health. Subsequently, the development of a fast and simple method for the CL quantificationemployed to detect its abuse in livestock production is an attractive analytical challenge.The proposed method was based on the use of a screen printed carbon electrode (SPCE) modifiedwith multiwall carbon nanotubes (MWCNTs) in a matrix of chitosan (CH) on gold nanoparticles (AuNPs)previously incorporated by electrodeposition process. The MWCNTs-CH/AuNPs/SPCE was characterizedby cyclic voltammetry, X-ray diffraction and scan electron microscopy.With the aim to carry out the CL determination, a pre-concentration procedure was required. For thispurpose, we employed anti-CL antibodies immobilized on magnetic microparticles (MPs) as bioaffinitysupport to capture the drug present in the sample. This support was exposed to a desorption process.Later, the electrochemical detection by square wave voltammetry (OSWV) was performed. The sensorexhibited excellent performances with a linear response range in concentrations from 0.01 to 6 ng mL−1.The detection and quantification limits obtained were 0.003 and 0.01 ng mL−1, respectively with an anal-ysis time of 19 min and a R.S.D. below 5.8%. As a conclusion, we can claim that the results obtained bythe proposed sensor were successful.Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Spotorno, Viviana G.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaElsevier2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5709Bertolino, Franco Adrián; Pereira, Sirley Vanesa; Spotorno, Viviana G.; Raba, Julio; Regiart, Daniel Matias Gaston; Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field; Elsevier; Sensors and Actuators B: Chemical; 188; 8-2013; 1241-12490925-4005enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S092540051300943Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2013.07.117info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:33:17Zoai:ri.conicet.gov.ar:11336/5709instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:33:17.775CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field
title Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field
spellingShingle Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field
Bertolino, Franco Adrián
Voltametric Sensor
Nanostructured Platform
Anabolic Drug
Food Safety
title_short Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field
title_full Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field
title_fullStr Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field
title_full_unstemmed Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field
title_sort Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field
dc.creator.none.fl_str_mv Bertolino, Franco Adrián
Pereira, Sirley Vanesa
Spotorno, Viviana G.
Raba, Julio
Regiart, Daniel Matias Gaston
author Bertolino, Franco Adrián
author_facet Bertolino, Franco Adrián
Pereira, Sirley Vanesa
Spotorno, Viviana G.
Raba, Julio
Regiart, Daniel Matias Gaston
author_role author
author2 Pereira, Sirley Vanesa
Spotorno, Viviana G.
Raba, Julio
Regiart, Daniel Matias Gaston
author2_role author
author
author
author
dc.subject.none.fl_str_mv Voltametric Sensor
Nanostructured Platform
Anabolic Drug
Food Safety
topic Voltametric Sensor
Nanostructured Platform
Anabolic Drug
Food Safety
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv tA sensitive electrochemical sensor for clenbuterol (CL) determination in bovine urine samples wasdesigned. This drug was originally developed for the treatment of respiratory pathologies. However,in the livestock industry, CL has been used as growth promoter because it stimulates the muscle growthand lipid degradation. The drug residues accumulated in animal tissues represent a potential risk forthe human health. Subsequently, the development of a fast and simple method for the CL quantificationemployed to detect its abuse in livestock production is an attractive analytical challenge.The proposed method was based on the use of a screen printed carbon electrode (SPCE) modifiedwith multiwall carbon nanotubes (MWCNTs) in a matrix of chitosan (CH) on gold nanoparticles (AuNPs)previously incorporated by electrodeposition process. The MWCNTs-CH/AuNPs/SPCE was characterizedby cyclic voltammetry, X-ray diffraction and scan electron microscopy.With the aim to carry out the CL determination, a pre-concentration procedure was required. For thispurpose, we employed anti-CL antibodies immobilized on magnetic microparticles (MPs) as bioaffinitysupport to capture the drug present in the sample. This support was exposed to a desorption process.Later, the electrochemical detection by square wave voltammetry (OSWV) was performed. The sensorexhibited excellent performances with a linear response range in concentrations from 0.01 to 6 ng mL−1.The detection and quantification limits obtained were 0.003 and 0.01 ng mL−1, respectively with an anal-ysis time of 19 min and a R.S.D. below 5.8%. As a conclusion, we can claim that the results obtained bythe proposed sensor were successful.
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Spotorno, Viviana G.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
description tA sensitive electrochemical sensor for clenbuterol (CL) determination in bovine urine samples wasdesigned. This drug was originally developed for the treatment of respiratory pathologies. However,in the livestock industry, CL has been used as growth promoter because it stimulates the muscle growthand lipid degradation. The drug residues accumulated in animal tissues represent a potential risk forthe human health. Subsequently, the development of a fast and simple method for the CL quantificationemployed to detect its abuse in livestock production is an attractive analytical challenge.The proposed method was based on the use of a screen printed carbon electrode (SPCE) modifiedwith multiwall carbon nanotubes (MWCNTs) in a matrix of chitosan (CH) on gold nanoparticles (AuNPs)previously incorporated by electrodeposition process. The MWCNTs-CH/AuNPs/SPCE was characterizedby cyclic voltammetry, X-ray diffraction and scan electron microscopy.With the aim to carry out the CL determination, a pre-concentration procedure was required. For thispurpose, we employed anti-CL antibodies immobilized on magnetic microparticles (MPs) as bioaffinitysupport to capture the drug present in the sample. This support was exposed to a desorption process.Later, the electrochemical detection by square wave voltammetry (OSWV) was performed. The sensorexhibited excellent performances with a linear response range in concentrations from 0.01 to 6 ng mL−1.The detection and quantification limits obtained were 0.003 and 0.01 ng mL−1, respectively with an anal-ysis time of 19 min and a R.S.D. below 5.8%. As a conclusion, we can claim that the results obtained bythe proposed sensor were successful.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5709
Bertolino, Franco Adrián; Pereira, Sirley Vanesa; Spotorno, Viviana G.; Raba, Julio; Regiart, Daniel Matias Gaston; Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field; Elsevier; Sensors and Actuators B: Chemical; 188; 8-2013; 1241-1249
0925-4005
url http://hdl.handle.net/11336/5709
identifier_str_mv Bertolino, Franco Adrián; Pereira, Sirley Vanesa; Spotorno, Viviana G.; Raba, Julio; Regiart, Daniel Matias Gaston; Nanostructured voltammetric sensor for ultra-trace anabolic drugdetermination in food safety field; Elsevier; Sensors and Actuators B: Chemical; 188; 8-2013; 1241-1249
0925-4005
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S092540051300943X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2013.07.117
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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