Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces

Autores
Regiart, Daniel Matias Gaston; Pereira, Sirley Vanesa; Spotorno, Viviana; Bertolino, Franco Adrián; Raba, Julio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study reports an accurate and sensitive strategy for zeranol (ZER) determination in bovine urine samples. ZER is a mycotoxin widely used as a synthetic growth promoter in the livestock production whose residues could present a potential risk for human health. Therefore, its use as an animal feed additive has been banned in most countries. ZER determination was accomplished using an electrochemical system in which bimetallic Au–Pt nanoparticles (Au–PtNPs) were electro-synthesized on a screen-printed carbon electrode (SPCE). The obtained Au–PtNP platform was immunofunctionalized using specific anti-ZER antibodies as a strategy to avoid potential interference. After biorecognition, ZER was directly oxidized and detected by square-wave voltammetry (SWV). The Au–PtNP surface was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and cyclic voltammetry (CV). The limit of detection calculated was 0.01 ng mL−1 with a wide linear range from 0.03 to 30 ng mL−1. This method promises to be suitable for ZER quantification in bovine urine samples ensuring food quality and safety, as well as consumer’s health.
Fil: Regiart, Daniel Matias Gaston. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Spotorno, Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Bertolino, Franco Adrián. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Materia
Food Safety
Zeranol
Voltammetric Immunosensing
Bimetallic Nanoparticle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14821
Acceder
id CONICETDig_666957ee35ed69eca26229420c096f95
oai_identifier_str oai:ri.conicet.gov.ar:11336/14821
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfacesRegiart, Daniel Matias GastonPereira, Sirley VanesaSpotorno, VivianaBertolino, Franco AdriánRaba, JulioFood SafetyZeranolVoltammetric ImmunosensingBimetallic Nanoparticlehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This study reports an accurate and sensitive strategy for zeranol (ZER) determination in bovine urine samples. ZER is a mycotoxin widely used as a synthetic growth promoter in the livestock production whose residues could present a potential risk for human health. Therefore, its use as an animal feed additive has been banned in most countries. ZER determination was accomplished using an electrochemical system in which bimetallic Au–Pt nanoparticles (Au–PtNPs) were electro-synthesized on a screen-printed carbon electrode (SPCE). The obtained Au–PtNP platform was immunofunctionalized using specific anti-ZER antibodies as a strategy to avoid potential interference. After biorecognition, ZER was directly oxidized and detected by square-wave voltammetry (SWV). The Au–PtNP surface was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and cyclic voltammetry (CV). The limit of detection calculated was 0.01 ng mL−1 with a wide linear range from 0.03 to 30 ng mL−1. This method promises to be suitable for ZER quantification in bovine urine samples ensuring food quality and safety, as well as consumer’s health.Fil: Regiart, Daniel Matias Gaston. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Pereira, Sirley Vanesa. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Spotorno, Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; ArgentinaFil: Bertolino, Franco Adrián. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaRoyal Society Of Chemistry2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14821Regiart, Daniel Matias Gaston; Pereira, Sirley Vanesa; Spotorno, Viviana; Bertolino, Franco Adrián; Raba, Julio; Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces; Royal Society Of Chemistry; Analyst; 139; 18; 6-2014; 4702-47090003-2654enginfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2014/an/c4an00686k#!divAbstractinfo:eu-repo/semantics/altIdentifier/doi/10.1039/C4AN00686Kinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:41Zoai:ri.conicet.gov.ar:11336/14821instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:42.041CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces
title Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces
spellingShingle Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces
Regiart, Daniel Matias Gaston
Food Safety
Zeranol
Voltammetric Immunosensing
Bimetallic Nanoparticle
title_short Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces
title_full Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces
title_fullStr Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces
title_full_unstemmed Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces
title_sort Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces
dc.creator.none.fl_str_mv Regiart, Daniel Matias Gaston
Pereira, Sirley Vanesa
Spotorno, Viviana
Bertolino, Franco Adrián
Raba, Julio
author Regiart, Daniel Matias Gaston
author_facet Regiart, Daniel Matias Gaston
Pereira, Sirley Vanesa
Spotorno, Viviana
Bertolino, Franco Adrián
Raba, Julio
author_role author
author2 Pereira, Sirley Vanesa
Spotorno, Viviana
Bertolino, Franco Adrián
Raba, Julio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Food Safety
Zeranol
Voltammetric Immunosensing
Bimetallic Nanoparticle
topic Food Safety
Zeranol
Voltammetric Immunosensing
Bimetallic Nanoparticle
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study reports an accurate and sensitive strategy for zeranol (ZER) determination in bovine urine samples. ZER is a mycotoxin widely used as a synthetic growth promoter in the livestock production whose residues could present a potential risk for human health. Therefore, its use as an animal feed additive has been banned in most countries. ZER determination was accomplished using an electrochemical system in which bimetallic Au–Pt nanoparticles (Au–PtNPs) were electro-synthesized on a screen-printed carbon electrode (SPCE). The obtained Au–PtNP platform was immunofunctionalized using specific anti-ZER antibodies as a strategy to avoid potential interference. After biorecognition, ZER was directly oxidized and detected by square-wave voltammetry (SWV). The Au–PtNP surface was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and cyclic voltammetry (CV). The limit of detection calculated was 0.01 ng mL−1 with a wide linear range from 0.03 to 30 ng mL−1. This method promises to be suitable for ZER quantification in bovine urine samples ensuring food quality and safety, as well as consumer’s health.
Fil: Regiart, Daniel Matias Gaston. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Spotorno, Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Bertolino, Franco Adrián. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
description This study reports an accurate and sensitive strategy for zeranol (ZER) determination in bovine urine samples. ZER is a mycotoxin widely used as a synthetic growth promoter in the livestock production whose residues could present a potential risk for human health. Therefore, its use as an animal feed additive has been banned in most countries. ZER determination was accomplished using an electrochemical system in which bimetallic Au–Pt nanoparticles (Au–PtNPs) were electro-synthesized on a screen-printed carbon electrode (SPCE). The obtained Au–PtNP platform was immunofunctionalized using specific anti-ZER antibodies as a strategy to avoid potential interference. After biorecognition, ZER was directly oxidized and detected by square-wave voltammetry (SWV). The Au–PtNP surface was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and cyclic voltammetry (CV). The limit of detection calculated was 0.01 ng mL−1 with a wide linear range from 0.03 to 30 ng mL−1. This method promises to be suitable for ZER quantification in bovine urine samples ensuring food quality and safety, as well as consumer’s health.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14821
Regiart, Daniel Matias Gaston; Pereira, Sirley Vanesa; Spotorno, Viviana; Bertolino, Franco Adrián; Raba, Julio; Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces; Royal Society Of Chemistry; Analyst; 139; 18; 6-2014; 4702-4709
0003-2654
url http://hdl.handle.net/11336/14821
identifier_str_mv Regiart, Daniel Matias Gaston; Pereira, Sirley Vanesa; Spotorno, Viviana; Bertolino, Franco Adrián; Raba, Julio; Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces; Royal Society Of Chemistry; Analyst; 139; 18; 6-2014; 4702-4709
0003-2654
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2014/an/c4an00686k#!divAbstract
info:eu-repo/semantics/altIdentifier/doi/10.1039/C4AN00686K
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society Of Chemistry
publisher.none.fl_str_mv Royal Society Of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269596058386432
score 13.13397

Publicaciones similares