Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species

Autores
Arizio, Carla Marcela; Costa Tártara, Sabrina María; Zunino, Ignacio Martin; Manifesto, Maria Marcela
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an "exon-primed intron-crossing" (EPIC) approach, fourteen pairs of primers were designed, on five structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to define partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identified several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplification gradient across wild Ipomoea species validated their transferability.
Fil: Arizio, Carla Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Costa Tártara, Sabrina María. Universidad Nacional de Luján. Departamento de Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zunino, Ignacio Martin. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Norte; Argentina
Fil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Materia
ANTHOCYANIN
CANDIDATE
GENES
ILP-CG
MARKERS
IPOMOEA
BATATAS
GENETIC
DIVERSITY
WILD
IPOMOEAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/206117
Acceder
id CONICETDig_f2ea8389ec539d22d4a79cae19643fc1
oai_identifier_str oai:ri.conicet.gov.ar:11336/206117
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea speciesArizio, Carla MarcelaCosta Tártara, Sabrina MaríaZunino, Ignacio MartinManifesto, Maria MarcelaANTHOCYANINCANDIDATEGENESILP-CGMARKERSIPOMOEABATATASGENETICDIVERSITYWILDIPOMOEAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an "exon-primed intron-crossing" (EPIC) approach, fourteen pairs of primers were designed, on five structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to define partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identified several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplification gradient across wild Ipomoea species validated their transferability.Fil: Arizio, Carla Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; ArgentinaFil: Costa Tártara, Sabrina María. Universidad Nacional de Luján. Departamento de Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zunino, Ignacio Martin. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Norte; ArgentinaFil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; ArgentinaAcademia Brasileira de Ciencias2022-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/206117Arizio, Carla Marcela; Costa Tártara, Sabrina María; Zunino, Ignacio Martin; Manifesto, Maria Marcela; Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 94; 3; 10-2022; 1-210001-37651678-2690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202220210672info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:53Zoai:ri.conicet.gov.ar:11336/206117instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:54.171CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
title Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
spellingShingle Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
Arizio, Carla Marcela
ANTHOCYANIN
CANDIDATE
GENES
ILP-CG
MARKERS
IPOMOEA
BATATAS
GENETIC
DIVERSITY
WILD
IPOMOEAS
title_short Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
title_full Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
title_fullStr Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
title_full_unstemmed Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
title_sort Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
dc.creator.none.fl_str_mv Arizio, Carla Marcela
Costa Tártara, Sabrina María
Zunino, Ignacio Martin
Manifesto, Maria Marcela
author Arizio, Carla Marcela
author_facet Arizio, Carla Marcela
Costa Tártara, Sabrina María
Zunino, Ignacio Martin
Manifesto, Maria Marcela
author_role author
author2 Costa Tártara, Sabrina María
Zunino, Ignacio Martin
Manifesto, Maria Marcela
author2_role author
author
author
dc.subject.none.fl_str_mv ANTHOCYANIN
CANDIDATE
GENES
ILP-CG
MARKERS
IPOMOEA
BATATAS
GENETIC
DIVERSITY
WILD
IPOMOEAS
topic ANTHOCYANIN
CANDIDATE
GENES
ILP-CG
MARKERS
IPOMOEA
BATATAS
GENETIC
DIVERSITY
WILD
IPOMOEAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an "exon-primed intron-crossing" (EPIC) approach, fourteen pairs of primers were designed, on five structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to define partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identified several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplification gradient across wild Ipomoea species validated their transferability.
Fil: Arizio, Carla Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Costa Tártara, Sabrina María. Universidad Nacional de Luján. Departamento de Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zunino, Ignacio Martin. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Norte; Argentina
Fil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
description The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an "exon-primed intron-crossing" (EPIC) approach, fourteen pairs of primers were designed, on five structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to define partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identified several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplification gradient across wild Ipomoea species validated their transferability.
publishDate 2022
dc.date.none.fl_str_mv 2022-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/206117
Arizio, Carla Marcela; Costa Tártara, Sabrina María; Zunino, Ignacio Martin; Manifesto, Maria Marcela; Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 94; 3; 10-2022; 1-21
0001-3765
1678-2690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/206117
identifier_str_mv Arizio, Carla Marcela; Costa Tártara, Sabrina María; Zunino, Ignacio Martin; Manifesto, Maria Marcela; Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 94; 3; 10-2022; 1-21
0001-3765
1678-2690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202220210672
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciencias
publisher.none.fl_str_mv Academia Brasileira de Ciencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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