Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications

Autores
Lanzavecchia, Silvia Beatriz; Juri, Marianela Lucía; Bonomi, Angelica; Gomulski, Ludvik; Scannapieco, Alejandra Carla; Segura, Diego Fernando; Malacrida, Anna; Cladera, Jorge Luis; Gasperi, Giuliano
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the American continent and limits the access of products to international markets. The use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of its population structure. Results: We developed microsatellite markers for A. fraterculus from four genomic libraries, which were enriched in CA, CAA, GA and CAT microsatellite motifs. Fifty microsatellite regions were evaluated and 14 loci were selected for population genetics studies. Genotypes of 122 individuals sampled from four A. fraterculus populations were analyzed. The level of polymorphism ranged from three to 13 alleles per locus and the mean expected heterozygosity ranged from 0.60 to 0.64. Comparison between allelic and genotypic frequencies showed significant differences among all pairs of populations. Conclusions: This novel set of microsatellite markers provides valuable information for the description of genetic variability and population structure of wild populations and laboratory strains of A. fraterculus. This information will be used to identify and characterize candidate strains suitable to implement effective pest control strategies and might represent a first step towards having a more comprehensive knowledge about the genetics of this pest.
Instituto de Genética
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina
Fil: Juri, Marianela Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bonomi, Angelica. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina
Fil: Gasperi, Giuliano. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia
Fil: Gomulski, Ludvik. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia
Fil: Malacrida, Anna. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia
Fuente
BMC Genetics 15 (Suppl 2) : S13 (2014)
Materia
Marcadores Genéticos
Tephritidae
Anastrepha fraterculus
Control Biológico
Genetic Markers
Biological Control
Mosca de la fruta
Técnica del insecto estéril
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3414
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oai_identifier_str oai:localhost:20.500.12123/3414
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applicationsLanzavecchia, Silvia BeatrizJuri, Marianela LucíaBonomi, AngelicaGomulski, LudvikScannapieco, Alejandra CarlaSegura, Diego FernandoMalacrida, AnnaCladera, Jorge LuisGasperi, GiulianoMarcadores GenéticosTephritidaeAnastrepha fraterculusControl BiológicoGenetic MarkersBiological ControlMosca de la frutaTécnica del insecto estérilBackground: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the American continent and limits the access of products to international markets. The use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of its population structure. Results: We developed microsatellite markers for A. fraterculus from four genomic libraries, which were enriched in CA, CAA, GA and CAT microsatellite motifs. Fifty microsatellite regions were evaluated and 14 loci were selected for population genetics studies. Genotypes of 122 individuals sampled from four A. fraterculus populations were analyzed. The level of polymorphism ranged from three to 13 alleles per locus and the mean expected heterozygosity ranged from 0.60 to 0.64. Comparison between allelic and genotypic frequencies showed significant differences among all pairs of populations. Conclusions: This novel set of microsatellite markers provides valuable information for the description of genetic variability and population structure of wild populations and laboratory strains of A. fraterculus. This information will be used to identify and characterize candidate strains suitable to implement effective pest control strategies and might represent a first step towards having a more comprehensive knowledge about the genetics of this pest.Instituto de GenéticaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; ArgentinaFil: Juri, Marianela Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bonomi, Angelica. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; ItaliaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; ArgentinaFil: Gasperi, Giuliano. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; ItaliaFil: Gomulski, Ludvik. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; ItaliaFil: Malacrida, Anna. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; ItaliaBioMed Central2018-09-19T18:10:32Z2018-09-19T18:10:32Z2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-15-S2-S13http://hdl.handle.net/20.500.12123/34141471-2156https://doi.org/10.1186/1471-2156-15-S2-S13BMC Genetics 15 (Suppl 2) : S13 (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:18Zoai:localhost:20.500.12123/3414instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:19.131INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications
title Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications
spellingShingle Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications
Lanzavecchia, Silvia Beatriz
Marcadores Genéticos
Tephritidae
Anastrepha fraterculus
Control Biológico
Genetic Markers
Biological Control
Mosca de la fruta
Técnica del insecto estéril
title_short Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications
title_full Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications
title_fullStr Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications
title_full_unstemmed Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications
title_sort Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications
dc.creator.none.fl_str_mv Lanzavecchia, Silvia Beatriz
Juri, Marianela Lucía
Bonomi, Angelica
Gomulski, Ludvik
Scannapieco, Alejandra Carla
Segura, Diego Fernando
Malacrida, Anna
Cladera, Jorge Luis
Gasperi, Giuliano
author Lanzavecchia, Silvia Beatriz
author_facet Lanzavecchia, Silvia Beatriz
Juri, Marianela Lucía
Bonomi, Angelica
Gomulski, Ludvik
Scannapieco, Alejandra Carla
Segura, Diego Fernando
Malacrida, Anna
Cladera, Jorge Luis
Gasperi, Giuliano
author_role author
author2 Juri, Marianela Lucía
Bonomi, Angelica
Gomulski, Ludvik
Scannapieco, Alejandra Carla
Segura, Diego Fernando
Malacrida, Anna
Cladera, Jorge Luis
Gasperi, Giuliano
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Marcadores Genéticos
Tephritidae
Anastrepha fraterculus
Control Biológico
Genetic Markers
Biological Control
Mosca de la fruta
Técnica del insecto estéril
topic Marcadores Genéticos
Tephritidae
Anastrepha fraterculus
Control Biológico
Genetic Markers
Biological Control
Mosca de la fruta
Técnica del insecto estéril
dc.description.none.fl_txt_mv Background: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the American continent and limits the access of products to international markets. The use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of its population structure. Results: We developed microsatellite markers for A. fraterculus from four genomic libraries, which were enriched in CA, CAA, GA and CAT microsatellite motifs. Fifty microsatellite regions were evaluated and 14 loci were selected for population genetics studies. Genotypes of 122 individuals sampled from four A. fraterculus populations were analyzed. The level of polymorphism ranged from three to 13 alleles per locus and the mean expected heterozygosity ranged from 0.60 to 0.64. Comparison between allelic and genotypic frequencies showed significant differences among all pairs of populations. Conclusions: This novel set of microsatellite markers provides valuable information for the description of genetic variability and population structure of wild populations and laboratory strains of A. fraterculus. This information will be used to identify and characterize candidate strains suitable to implement effective pest control strategies and might represent a first step towards having a more comprehensive knowledge about the genetics of this pest.
Instituto de Genética
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina
Fil: Juri, Marianela Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bonomi, Angelica. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina
Fil: Gasperi, Giuliano. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia
Fil: Gomulski, Ludvik. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia
Fil: Malacrida, Anna. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia
description Background: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the American continent and limits the access of products to international markets. The use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of its population structure. Results: We developed microsatellite markers for A. fraterculus from four genomic libraries, which were enriched in CA, CAA, GA and CAT microsatellite motifs. Fifty microsatellite regions were evaluated and 14 loci were selected for population genetics studies. Genotypes of 122 individuals sampled from four A. fraterculus populations were analyzed. The level of polymorphism ranged from three to 13 alleles per locus and the mean expected heterozygosity ranged from 0.60 to 0.64. Comparison between allelic and genotypic frequencies showed significant differences among all pairs of populations. Conclusions: This novel set of microsatellite markers provides valuable information for the description of genetic variability and population structure of wild populations and laboratory strains of A. fraterculus. This information will be used to identify and characterize candidate strains suitable to implement effective pest control strategies and might represent a first step towards having a more comprehensive knowledge about the genetics of this pest.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-01
2018-09-19T18:10:32Z
2018-09-19T18:10:32Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-15-S2-S13
http://hdl.handle.net/20.500.12123/3414
1471-2156
https://doi.org/10.1186/1471-2156-15-S2-S13
url https://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-15-S2-S13
http://hdl.handle.net/20.500.12123/3414
https://doi.org/10.1186/1471-2156-15-S2-S13
identifier_str_mv 1471-2156
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv BMC Genetics 15 (Suppl 2) : S13 (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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