Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications

Autores
Lanzavecchia, Silvia Beatriz; Juri, Marianela Lucia; Bonomi, Angelica; Gomulski, Ludvik; Scannapieco, Alejandra Carla; Segura, Diego Fernando; Malacrida, Anna; Cladera, Jorge Luis; Gasperi, Giuliano
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the American continent and limits the access of products to international markets. The use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of its population structure. Results: We developed microsatellite markers for A. fraterculus from four genomic libraries, which were enriched in CA, CAA, GA and CAT microsatellite motifs. Fifty microsatellite regions were evaluated and 14 loci were selected for population genetics studies. Genotypes of 122 individuals sampled from four A. fraterculus populations were analyzed. The level of polymorphism ranged from three to 13 alleles per locus and the mean expected heterozygosity ranged from 0.60 to 0.64. Comparison between allelic and genotypic frequencies showed significant differences among all pairs of populations. Conclusions: This novel set of microsatellite markers provides valuable information for the description of genetic variability and population structure of wild populations and laboratory strains of A. fraterculus. This information will be used to identify and characterize candidate strains suitable to implement effective pest control strategies and might represent a first step towards having a more comprehensive knowledge about the genetics of this pest.
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Juri, Marianela Lucia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bonomi, Angelica. Dipartimento Di Biologia E Biotecnologie L. Spallanz; Italia
Fil: Gomulski, Ludvik. Dipartimento Di Biologia E Biotecnologie L. Spallanz; Italia
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Malacrida, Anna. Dipartimento Di Biologia E Biotecnologie L. Spallanzan; Italia
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gasperi, Giuliano. 3 Dipartimento Di Biologia E Biotecnologie L. Spallanz; Italia
Materia
Anastrepha fraterculus
Microsatellite markers
Genetic variability
SIT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37206
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/37206
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applicationsLanzavecchia, Silvia BeatrizJuri, Marianela LuciaBonomi, AngelicaGomulski, LudvikScannapieco, Alejandra CarlaSegura, Diego FernandoMalacrida, AnnaCladera, Jorge LuisGasperi, GiulianoAnastrepha fraterculusMicrosatellite markersGenetic variabilitySIThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the American continent and limits the access of products to international markets. The use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of its population structure. Results: We developed microsatellite markers for A. fraterculus from four genomic libraries, which were enriched in CA, CAA, GA and CAT microsatellite motifs. Fifty microsatellite regions were evaluated and 14 loci were selected for population genetics studies. Genotypes of 122 individuals sampled from four A. fraterculus populations were analyzed. The level of polymorphism ranged from three to 13 alleles per locus and the mean expected heterozygosity ranged from 0.60 to 0.64. Comparison between allelic and genotypic frequencies showed significant differences among all pairs of populations. Conclusions: This novel set of microsatellite markers provides valuable information for the description of genetic variability and population structure of wild populations and laboratory strains of A. fraterculus. This information will be used to identify and characterize candidate strains suitable to implement effective pest control strategies and might represent a first step towards having a more comprehensive knowledge about the genetics of this pest.Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Juri, Marianela Lucia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bonomi, Angelica. Dipartimento Di Biologia E Biotecnologie L. Spallanz; ItaliaFil: Gomulski, Ludvik. Dipartimento Di Biologia E Biotecnologie L. Spallanz; ItaliaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Malacrida, Anna. Dipartimento Di Biologia E Biotecnologie L. Spallanzan; ItaliaFil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gasperi, Giuliano. 3 Dipartimento Di Biologia E Biotecnologie L. Spallanz; ItaliaBioMed Central2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37206Lanzavecchia, Silvia Beatriz; Juri, Marianela Lucia; Bonomi, Angelica; Gomulski, Ludvik; Scannapieco, Alejandra Carla; et al.; Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications; BioMed Central; BMC Genetics; 15; Suppl 2; 12-2014; 1-81471-2156CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2156-15-S2-S13info:eu-repo/semantics/altIdentifier/url/https://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-15-S2-S13info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255783/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:59:08Zoai:ri.conicet.gov.ar:11336/37206instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:59:09.277CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications
title Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications
spellingShingle Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications
Lanzavecchia, Silvia Beatriz
Anastrepha fraterculus
Microsatellite markers
Genetic variability
SIT
title_short Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications
title_full Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications
title_fullStr Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications
title_full_unstemmed Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications
title_sort Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications
dc.creator.none.fl_str_mv Lanzavecchia, Silvia Beatriz
Juri, Marianela Lucia
Bonomi, Angelica
Gomulski, Ludvik
Scannapieco, Alejandra Carla
Segura, Diego Fernando
Malacrida, Anna
Cladera, Jorge Luis
Gasperi, Giuliano
author Lanzavecchia, Silvia Beatriz
author_facet Lanzavecchia, Silvia Beatriz
Juri, Marianela Lucia
Bonomi, Angelica
Gomulski, Ludvik
Scannapieco, Alejandra Carla
Segura, Diego Fernando
Malacrida, Anna
Cladera, Jorge Luis
Gasperi, Giuliano
author_role author
author2 Juri, Marianela Lucia
Bonomi, Angelica
Gomulski, Ludvik
Scannapieco, Alejandra Carla
Segura, Diego Fernando
Malacrida, Anna
Cladera, Jorge Luis
Gasperi, Giuliano
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anastrepha fraterculus
Microsatellite markers
Genetic variability
SIT
topic Anastrepha fraterculus
Microsatellite markers
Genetic variability
SIT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the American continent and limits the access of products to international markets. The use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of its population structure. Results: We developed microsatellite markers for A. fraterculus from four genomic libraries, which were enriched in CA, CAA, GA and CAT microsatellite motifs. Fifty microsatellite regions were evaluated and 14 loci were selected for population genetics studies. Genotypes of 122 individuals sampled from four A. fraterculus populations were analyzed. The level of polymorphism ranged from three to 13 alleles per locus and the mean expected heterozygosity ranged from 0.60 to 0.64. Comparison between allelic and genotypic frequencies showed significant differences among all pairs of populations. Conclusions: This novel set of microsatellite markers provides valuable information for the description of genetic variability and population structure of wild populations and laboratory strains of A. fraterculus. This information will be used to identify and characterize candidate strains suitable to implement effective pest control strategies and might represent a first step towards having a more comprehensive knowledge about the genetics of this pest.
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Juri, Marianela Lucia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bonomi, Angelica. Dipartimento Di Biologia E Biotecnologie L. Spallanz; Italia
Fil: Gomulski, Ludvik. Dipartimento Di Biologia E Biotecnologie L. Spallanz; Italia
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Malacrida, Anna. Dipartimento Di Biologia E Biotecnologie L. Spallanzan; Italia
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gasperi, Giuliano. 3 Dipartimento Di Biologia E Biotecnologie L. Spallanz; Italia
description Background: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the American continent and limits the access of products to international markets. The use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of its population structure. Results: We developed microsatellite markers for A. fraterculus from four genomic libraries, which were enriched in CA, CAA, GA and CAT microsatellite motifs. Fifty microsatellite regions were evaluated and 14 loci were selected for population genetics studies. Genotypes of 122 individuals sampled from four A. fraterculus populations were analyzed. The level of polymorphism ranged from three to 13 alleles per locus and the mean expected heterozygosity ranged from 0.60 to 0.64. Comparison between allelic and genotypic frequencies showed significant differences among all pairs of populations. Conclusions: This novel set of microsatellite markers provides valuable information for the description of genetic variability and population structure of wild populations and laboratory strains of A. fraterculus. This information will be used to identify and characterize candidate strains suitable to implement effective pest control strategies and might represent a first step towards having a more comprehensive knowledge about the genetics of this pest.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37206
Lanzavecchia, Silvia Beatriz; Juri, Marianela Lucia; Bonomi, Angelica; Gomulski, Ludvik; Scannapieco, Alejandra Carla; et al.; Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications; BioMed Central; BMC Genetics; 15; Suppl 2; 12-2014; 1-8
1471-2156
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37206
identifier_str_mv Lanzavecchia, Silvia Beatriz; Juri, Marianela Lucia; Bonomi, Angelica; Gomulski, Ludvik; Scannapieco, Alejandra Carla; et al.; Microsatellite markers from the 'South American fruit fly' Anastrepha fraterculus: A valuable tool for population genetic analysis and SIT applications; BioMed Central; BMC Genetics; 15; Suppl 2; 12-2014; 1-8
1471-2156
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2156-15-S2-S13
info:eu-repo/semantics/altIdentifier/url/https://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-15-S2-S13
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255783/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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