Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations

Autores
Gomulski, Ludvik; Vera, María Teresa; Lanzavecchia, Silvia Beatriz; Piccinno, Riccardo; Fiorenza, Giulia; De Luca, Daniel; Carrizo, Beatriz Noemi; Bouvet, Juan Pedro; Viana, Valeria Angelica; Cárceres, Carlos; Enkerlin, Walther; Malacrida, Anna; Gasperi, Giuliano
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The South American fruit fly Anastrepha fraterculus (Wiedmann) has a vast range extending from northern Mexico, through Central America, to South America where it is an extremely polyphagous pest of wild and cultivated fruits. It is a complex of cryptic species currently composed of eight recognised morphotypes: “Mexican”, “Venezuelan”, “Andean”, “Peruvian”, “Ecuadorian”, and the three Brazilian morphotypes “Brazilian-1”, “Brazilian-2”, and “Brazilian-3”. Molecular markers that can identify the member species of the complex are crucial for the implementation of effective pest control measures, such as the sterile insect technique. The object of this study was to evaluate the use of the internal transcribed spacer 2 (ITS2) sequence for discriminating several members of the A. fraterculus cryptic species complex (Mexican, Peruvian, and Brazilian-1) and a related species, Anastrepha schultzi Blanchard. The analysis highlighted significant genetic differentiation between the evaluated morphotypes, allowed their discrimination within the A. fraterculus cryptic species complex, and provided new insights into their genetic relationships. The ITS2 marker provides a basis for the development of taxonomic keys for the discrimination of the cryptic taxa within the A. fraterculus cryptic species complex. ITS2 also represents an important marker for the poorly studied species A. schultzi.
Instituto de Genética
Fil: Gomulski, Ludvik. University of Pavia. Department of Biology and Biotechnology; Italia
Fil: Vera, María Teresa. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Piccinno, Riccardo. University of Pavia. Department of Biology and Biotechnology; Italia
Fil: Fiorenza, Giulia. University of Pavia. Department of Biology and Biotechnology; Italia
Fil: De Luca, Daniel. University of Pavia. Department of Biology and Biotechnology; Italia
Fil: Carrizo, Beatriz Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Bouvet, Juan Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia. Grupo de Protección Vegetal; Argentina
Fil: Viana, Valeria Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia. Grupo de Protección Vegetal; Argentina
Fil: Cárceres, Carlos. Joint FAO/IAEA Centre. Insect Pest Control Section; Austria
Fil: Enkerlin, Walther. Joint FAO/IAEA Centre. Insect Pest Control Section; Austria
Fil: Malacrida, Anna. University of Pavia. Department of Biology and Biotechnology; Italia
Fil: Gasperi, Giuliano. University of Pavia. Department of Biology and Biotechnology; Italia
Fuente
Insects 15 (10) : 748 (October 2024)
Materia
Anastrepha fraterculus
Marcadores Genéticos
Variación Genética
Genetic Markers
Genetic Variation
Anastrepha schultzi
Morfotipos
Morphotypes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21879
Acceder
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spelling Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populationsGomulski, LudvikVera, María TeresaLanzavecchia, Silvia BeatrizPiccinno, RiccardoFiorenza, GiuliaDe Luca, DanielCarrizo, Beatriz NoemiBouvet, Juan PedroViana, Valeria AngelicaCárceres, CarlosEnkerlin, WaltherMalacrida, AnnaGasperi, GiulianoAnastrepha fraterculusMarcadores GenéticosVariación GenéticaGenetic MarkersGenetic VariationAnastrepha schultziMorfotiposMorphotypesThe South American fruit fly Anastrepha fraterculus (Wiedmann) has a vast range extending from northern Mexico, through Central America, to South America where it is an extremely polyphagous pest of wild and cultivated fruits. It is a complex of cryptic species currently composed of eight recognised morphotypes: “Mexican”, “Venezuelan”, “Andean”, “Peruvian”, “Ecuadorian”, and the three Brazilian morphotypes “Brazilian-1”, “Brazilian-2”, and “Brazilian-3”. Molecular markers that can identify the member species of the complex are crucial for the implementation of effective pest control measures, such as the sterile insect technique. The object of this study was to evaluate the use of the internal transcribed spacer 2 (ITS2) sequence for discriminating several members of the A. fraterculus cryptic species complex (Mexican, Peruvian, and Brazilian-1) and a related species, Anastrepha schultzi Blanchard. The analysis highlighted significant genetic differentiation between the evaluated morphotypes, allowed their discrimination within the A. fraterculus cryptic species complex, and provided new insights into their genetic relationships. The ITS2 marker provides a basis for the development of taxonomic keys for the discrimination of the cryptic taxa within the A. fraterculus cryptic species complex. ITS2 also represents an important marker for the poorly studied species A. schultzi.Instituto de GenéticaFil: Gomulski, Ludvik. University of Pavia. Department of Biology and Biotechnology; ItaliaFil: Vera, María Teresa. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; ArgentinaFil: Piccinno, Riccardo. University of Pavia. Department of Biology and Biotechnology; ItaliaFil: Fiorenza, Giulia. University of Pavia. Department of Biology and Biotechnology; ItaliaFil: De Luca, Daniel. University of Pavia. Department of Biology and Biotechnology; ItaliaFil: Carrizo, Beatriz Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Bouvet, Juan Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia. Grupo de Protección Vegetal; ArgentinaFil: Viana, Valeria Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia. Grupo de Protección Vegetal; ArgentinaFil: Cárceres, Carlos. Joint FAO/IAEA Centre. Insect Pest Control Section; AustriaFil: Enkerlin, Walther. Joint FAO/IAEA Centre. Insect Pest Control Section; AustriaFil: Malacrida, Anna. University of Pavia. Department of Biology and Biotechnology; ItaliaFil: Gasperi, Giuliano. University of Pavia. Department of Biology and Biotechnology; ItaliaMDPI2025-04-03T11:58:17Z2025-04-03T11:58:17Z2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21879https://www.mdpi.com/2075-4450/15/10/7482075-4450https://doi.org/10.3390/insects15100748Insects 15 (10) : 748 (October 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-30T11:25:14Zoai:localhost:20.500.12123/21879instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-30 11:25:14.732INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations
title Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations
spellingShingle Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations
Gomulski, Ludvik
Anastrepha fraterculus
Marcadores Genéticos
Variación Genética
Genetic Markers
Genetic Variation
Anastrepha schultzi
Morfotipos
Morphotypes
title_short Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations
title_full Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations
title_fullStr Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations
title_full_unstemmed Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations
title_sort Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations
dc.creator.none.fl_str_mv Gomulski, Ludvik
Vera, María Teresa
Lanzavecchia, Silvia Beatriz
Piccinno, Riccardo
Fiorenza, Giulia
De Luca, Daniel
Carrizo, Beatriz Noemi
Bouvet, Juan Pedro
Viana, Valeria Angelica
Cárceres, Carlos
Enkerlin, Walther
Malacrida, Anna
Gasperi, Giuliano
author Gomulski, Ludvik
author_facet Gomulski, Ludvik
Vera, María Teresa
Lanzavecchia, Silvia Beatriz
Piccinno, Riccardo
Fiorenza, Giulia
De Luca, Daniel
Carrizo, Beatriz Noemi
Bouvet, Juan Pedro
Viana, Valeria Angelica
Cárceres, Carlos
Enkerlin, Walther
Malacrida, Anna
Gasperi, Giuliano
author_role author
author2 Vera, María Teresa
Lanzavecchia, Silvia Beatriz
Piccinno, Riccardo
Fiorenza, Giulia
De Luca, Daniel
Carrizo, Beatriz Noemi
Bouvet, Juan Pedro
Viana, Valeria Angelica
Cárceres, Carlos
Enkerlin, Walther
Malacrida, Anna
Gasperi, Giuliano
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anastrepha fraterculus
Marcadores Genéticos
Variación Genética
Genetic Markers
Genetic Variation
Anastrepha schultzi
Morfotipos
Morphotypes
topic Anastrepha fraterculus
Marcadores Genéticos
Variación Genética
Genetic Markers
Genetic Variation
Anastrepha schultzi
Morfotipos
Morphotypes
dc.description.none.fl_txt_mv The South American fruit fly Anastrepha fraterculus (Wiedmann) has a vast range extending from northern Mexico, through Central America, to South America where it is an extremely polyphagous pest of wild and cultivated fruits. It is a complex of cryptic species currently composed of eight recognised morphotypes: “Mexican”, “Venezuelan”, “Andean”, “Peruvian”, “Ecuadorian”, and the three Brazilian morphotypes “Brazilian-1”, “Brazilian-2”, and “Brazilian-3”. Molecular markers that can identify the member species of the complex are crucial for the implementation of effective pest control measures, such as the sterile insect technique. The object of this study was to evaluate the use of the internal transcribed spacer 2 (ITS2) sequence for discriminating several members of the A. fraterculus cryptic species complex (Mexican, Peruvian, and Brazilian-1) and a related species, Anastrepha schultzi Blanchard. The analysis highlighted significant genetic differentiation between the evaluated morphotypes, allowed their discrimination within the A. fraterculus cryptic species complex, and provided new insights into their genetic relationships. The ITS2 marker provides a basis for the development of taxonomic keys for the discrimination of the cryptic taxa within the A. fraterculus cryptic species complex. ITS2 also represents an important marker for the poorly studied species A. schultzi.
Instituto de Genética
Fil: Gomulski, Ludvik. University of Pavia. Department of Biology and Biotechnology; Italia
Fil: Vera, María Teresa. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Piccinno, Riccardo. University of Pavia. Department of Biology and Biotechnology; Italia
Fil: Fiorenza, Giulia. University of Pavia. Department of Biology and Biotechnology; Italia
Fil: De Luca, Daniel. University of Pavia. Department of Biology and Biotechnology; Italia
Fil: Carrizo, Beatriz Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Bouvet, Juan Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia. Grupo de Protección Vegetal; Argentina
Fil: Viana, Valeria Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia. Grupo de Protección Vegetal; Argentina
Fil: Cárceres, Carlos. Joint FAO/IAEA Centre. Insect Pest Control Section; Austria
Fil: Enkerlin, Walther. Joint FAO/IAEA Centre. Insect Pest Control Section; Austria
Fil: Malacrida, Anna. University of Pavia. Department of Biology and Biotechnology; Italia
Fil: Gasperi, Giuliano. University of Pavia. Department of Biology and Biotechnology; Italia
description The South American fruit fly Anastrepha fraterculus (Wiedmann) has a vast range extending from northern Mexico, through Central America, to South America where it is an extremely polyphagous pest of wild and cultivated fruits. It is a complex of cryptic species currently composed of eight recognised morphotypes: “Mexican”, “Venezuelan”, “Andean”, “Peruvian”, “Ecuadorian”, and the three Brazilian morphotypes “Brazilian-1”, “Brazilian-2”, and “Brazilian-3”. Molecular markers that can identify the member species of the complex are crucial for the implementation of effective pest control measures, such as the sterile insect technique. The object of this study was to evaluate the use of the internal transcribed spacer 2 (ITS2) sequence for discriminating several members of the A. fraterculus cryptic species complex (Mexican, Peruvian, and Brazilian-1) and a related species, Anastrepha schultzi Blanchard. The analysis highlighted significant genetic differentiation between the evaluated morphotypes, allowed their discrimination within the A. fraterculus cryptic species complex, and provided new insights into their genetic relationships. The ITS2 marker provides a basis for the development of taxonomic keys for the discrimination of the cryptic taxa within the A. fraterculus cryptic species complex. ITS2 also represents an important marker for the poorly studied species A. schultzi.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
2025-04-03T11:58:17Z
2025-04-03T11:58:17Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21879
https://www.mdpi.com/2075-4450/15/10/748
2075-4450
https://doi.org/10.3390/insects15100748
url http://hdl.handle.net/20.500.12123/21879
https://www.mdpi.com/2075-4450/15/10/748
https://doi.org/10.3390/insects15100748
identifier_str_mv 2075-4450
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Insects 15 (10) : 748 (October 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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