Biochemical differences in the skin of two blueberries (Vaccinium corymbosum) varieties with contrasting firmness: implication of ions, metabolites and cell wall related proteins i...

Autores: Montecchiarini, Marina Lucía; Silva-Sanzana, C.; Valderramo, L.; Alemano, Sergio Gabriel; Gollan, Agustín María; Rivadeneira, Maria Fernanda; Bello, Fernando; Vazquez, Daniel Eduardo; Blanco-Herrera, F.; Podesta, Florencio Esteban; Tripodi, Karina Eva Josefina
Año de publicación: 2021
Idioma: inglés
Tipo de recurso: artículo
Estado: versión aceptada
Descripción: The pursuit of firmer and better-quality blueberries is a continuous task that aims at a more profitable production. To this end it is essential to understand the biological processes linked to fruit firmness, which may diverge among tissues. By contrasting varieties with opposing firmness, we were able to elucidate events that, taking place at immature stage, lay the foundation to produce a firmer ripe fruit. A deep analysis of blueberry skin was carried out, involving diverse comparative approaches including proteomics and metabolomics coupled to immunolocalization assays. In‘O'Neal’ (low firmness) enhanced levels of aquaporins, expansins and pectin esterases at the green stage were found to be critical in distinguishing it from ‘Emerald’ (high firmness). The latter featured higher levels of ABA, low methyl esterified pectins in tricellular junctions and high levels of catechin at this stage. Meanwhile, in ‘Emerald' ’s ripe fruit epicarp, several mechanisms of cell wall reinforcement such as calcium and probably boron bridges, appear to be more prominent than in ‘O’Neal’. This study highlights the importance of cell wall reorganization and structure, abundance of specific metabolites, water status, and hormonal signalling in connection to fruit firmness. These findings result particularly valuable in order to improve the fertilization procedures or in the search of molecular markers related with firmness.
EEA Concordia
Fil: Montecchiarini, Marina Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina
Fil: Silva-Sanzana, C. Universidad Andrés Bello. Facultad de Ciencias de la Vida. Centro de Biotecnología Vegetal; Chile
Fil: Valderramo, L. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina
Fil: Alemano, Sergio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales; Argentina
Fil: Gollán, Agustín María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Rivadeneira, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Blanco-Herrera, F. Universidad Andrés Bello. Facultad de Ciencias de la Vida. Centro de Biotecnología Vegetal; Chile
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina
Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina
Fuente: Plant Physiology and Biochemistry (Available online 13 March 2021)
Materia: Arándano
Vaccinium corymbosum
Variedades
Proteínas
Pared Celular
Blueberries
Varieties
Proteins
Cell Walls
ABA
Abscisic Acid
Nivel de accesibilidad: acceso restringido
Condiciones de uso
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/8901

Acceder

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spelling	Biochemical differences in the skin of two blueberries (Vaccinium corymbosum) varieties with contrasting firmness: implication of ions, metabolites and cell wall related proteins in two developmental stagesMontecchiarini, Marina LucíaSilva-Sanzana, C.Valderramo, L.Alemano, Sergio GabrielGollan, Agustín MaríaRivadeneira, Maria FernandaBello, FernandoVazquez, Daniel EduardoBlanco-Herrera, F.Podesta, Florencio EstebanTripodi, Karina Eva JosefinaArándanoVaccinium corymbosumVariedadesProteínasPared CelularBlueberriesVarietiesProteinsCell WallsABAAbscisic AcidThe pursuit of firmer and better-quality blueberries is a continuous task that aims at a more profitable production. To this end it is essential to understand the biological processes linked to fruit firmness, which may diverge among tissues. By contrasting varieties with opposing firmness, we were able to elucidate events that, taking place at immature stage, lay the foundation to produce a firmer ripe fruit. A deep analysis of blueberry skin was carried out, involving diverse comparative approaches including proteomics and metabolomics coupled to immunolocalization assays. In‘O'Neal’ (low firmness) enhanced levels of aquaporins, expansins and pectin esterases at the green stage were found to be critical in distinguishing it from ‘Emerald’ (high firmness). The latter featured higher levels of ABA, low methyl esterified pectins in tricellular junctions and high levels of catechin at this stage. Meanwhile, in ‘Emerald' ’s ripe fruit epicarp, several mechanisms of cell wall reinforcement such as calcium and probably boron bridges, appear to be more prominent than in ‘O’Neal’. This study highlights the importance of cell wall reorganization and structure, abundance of specific metabolites, water status, and hormonal signalling in connection to fruit firmness. These findings result particularly valuable in order to improve the fertilization procedures or in the search of molecular markers related with firmness.EEA ConcordiaFil: Montecchiarini, Marina Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; ArgentinaFil: Silva-Sanzana, C. Universidad Andrés Bello. Facultad de Ciencias de la Vida. Centro de Biotecnología Vegetal; ChileFil: Valderramo, L. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; ArgentinaFil: Alemano, Sergio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales; ArgentinaFil: Gollán, Agustín María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Rivadeneira, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Blanco-Herrera, F. Universidad Andrés Bello. Facultad de Ciencias de la Vida. Centro de Biotecnología Vegetal; ChileFil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; ArgentinaFil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2023-03-162021-03-16T12:39:10Z2021-03-16T12:39:10Z2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8901https://www.sciencedirect.com/science/article/abs/pii/S09819428210014060981-9428https://doi.org/10.1016/j.plaphy.2021.03.016Plant Physiology and Biochemistry (Available online 13 March 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:09Zoai:localhost:20.500.12123/8901instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:10.115INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Biochemical differences in the skin of two blueberries (Vaccinium corymbosum) varieties with contrasting firmness: implication of ions, metabolites and cell wall related proteins in two developmental stages
title	Biochemical differences in the skin of two blueberries (Vaccinium corymbosum) varieties with contrasting firmness: implication of ions, metabolites and cell wall related proteins in two developmental stages
spellingShingle	Biochemical differences in the skin of two blueberries (Vaccinium corymbosum) varieties with contrasting firmness: implication of ions, metabolites and cell wall related proteins in two developmental stages Montecchiarini, Marina Lucía Arándano Vaccinium corymbosum Variedades Proteínas Pared Celular Blueberries Varieties Proteins Cell Walls ABA Abscisic Acid
title_short	Biochemical differences in the skin of two blueberries (Vaccinium corymbosum) varieties with contrasting firmness: implication of ions, metabolites and cell wall related proteins in two developmental stages
title_full	Biochemical differences in the skin of two blueberries (Vaccinium corymbosum) varieties with contrasting firmness: implication of ions, metabolites and cell wall related proteins in two developmental stages
title_fullStr	Biochemical differences in the skin of two blueberries (Vaccinium corymbosum) varieties with contrasting firmness: implication of ions, metabolites and cell wall related proteins in two developmental stages
title_full_unstemmed	Biochemical differences in the skin of two blueberries (Vaccinium corymbosum) varieties with contrasting firmness: implication of ions, metabolites and cell wall related proteins in two developmental stages
title_sort	Biochemical differences in the skin of two blueberries (Vaccinium corymbosum) varieties with contrasting firmness: implication of ions, metabolites and cell wall related proteins in two developmental stages
dc.creator.none.fl_str_mv	Montecchiarini, Marina Lucía Silva-Sanzana, C. Valderramo, L. Alemano, Sergio Gabriel Gollan, Agustín María Rivadeneira, Maria Fernanda Bello, Fernando Vazquez, Daniel Eduardo Blanco-Herrera, F. Podesta, Florencio Esteban Tripodi, Karina Eva Josefina
author	Montecchiarini, Marina Lucía
author_facet	Montecchiarini, Marina Lucía Silva-Sanzana, C. Valderramo, L. Alemano, Sergio Gabriel Gollan, Agustín María Rivadeneira, Maria Fernanda Bello, Fernando Vazquez, Daniel Eduardo Blanco-Herrera, F. Podesta, Florencio Esteban Tripodi, Karina Eva Josefina
author_role	author
author2	Silva-Sanzana, C. Valderramo, L. Alemano, Sergio Gabriel Gollan, Agustín María Rivadeneira, Maria Fernanda Bello, Fernando Vazquez, Daniel Eduardo Blanco-Herrera, F. Podesta, Florencio Esteban Tripodi, Karina Eva Josefina
author2_role	author author author author author author author author author author
dc.subject.none.fl_str_mv	Arándano Vaccinium corymbosum Variedades Proteínas Pared Celular Blueberries Varieties Proteins Cell Walls ABA Abscisic Acid
topic	Arándano Vaccinium corymbosum Variedades Proteínas Pared Celular Blueberries Varieties Proteins Cell Walls ABA Abscisic Acid
dc.description.none.fl_txt_mv	The pursuit of firmer and better-quality blueberries is a continuous task that aims at a more profitable production. To this end it is essential to understand the biological processes linked to fruit firmness, which may diverge among tissues. By contrasting varieties with opposing firmness, we were able to elucidate events that, taking place at immature stage, lay the foundation to produce a firmer ripe fruit. A deep analysis of blueberry skin was carried out, involving diverse comparative approaches including proteomics and metabolomics coupled to immunolocalization assays. In‘O'Neal’ (low firmness) enhanced levels of aquaporins, expansins and pectin esterases at the green stage were found to be critical in distinguishing it from ‘Emerald’ (high firmness). The latter featured higher levels of ABA, low methyl esterified pectins in tricellular junctions and high levels of catechin at this stage. Meanwhile, in ‘Emerald' ’s ripe fruit epicarp, several mechanisms of cell wall reinforcement such as calcium and probably boron bridges, appear to be more prominent than in ‘O’Neal’. This study highlights the importance of cell wall reorganization and structure, abundance of specific metabolites, water status, and hormonal signalling in connection to fruit firmness. These findings result particularly valuable in order to improve the fertilization procedures or in the search of molecular markers related with firmness. EEA Concordia Fil: Montecchiarini, Marina Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina Fil: Silva-Sanzana, C. Universidad Andrés Bello. Facultad de Ciencias de la Vida. Centro de Biotecnología Vegetal; Chile Fil: Valderramo, L. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina Fil: Alemano, Sergio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales; Argentina Fil: Gollán, Agustín María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Rivadeneira, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Blanco-Herrera, F. Universidad Andrés Bello. Facultad de Ciencias de la Vida. Centro de Biotecnología Vegetal; Chile Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina
description	The pursuit of firmer and better-quality blueberries is a continuous task that aims at a more profitable production. To this end it is essential to understand the biological processes linked to fruit firmness, which may diverge among tissues. By contrasting varieties with opposing firmness, we were able to elucidate events that, taking place at immature stage, lay the foundation to produce a firmer ripe fruit. A deep analysis of blueberry skin was carried out, involving diverse comparative approaches including proteomics and metabolomics coupled to immunolocalization assays. In‘O'Neal’ (low firmness) enhanced levels of aquaporins, expansins and pectin esterases at the green stage were found to be critical in distinguishing it from ‘Emerald’ (high firmness). The latter featured higher levels of ABA, low methyl esterified pectins in tricellular junctions and high levels of catechin at this stage. Meanwhile, in ‘Emerald' ’s ripe fruit epicarp, several mechanisms of cell wall reinforcement such as calcium and probably boron bridges, appear to be more prominent than in ‘O’Neal’. This study highlights the importance of cell wall reorganization and structure, abundance of specific metabolites, water status, and hormonal signalling in connection to fruit firmness. These findings result particularly valuable in order to improve the fertilization procedures or in the search of molecular markers related with firmness.
publishDate	2021
dc.date.none.fl_str_mv	2021-03-16T12:39:10Z 2021-03-16T12:39:10Z 2021-03 info:eu-repo/date/embargoEnd/2023-03-16
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	acceptedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/8901 https://www.sciencedirect.com/science/article/abs/pii/S0981942821001406 0981-9428 https://doi.org/10.1016/j.plaphy.2021.03.016
url	http://hdl.handle.net/20.500.12123/8901 https://www.sciencedirect.com/science/article/abs/pii/S0981942821001406 https://doi.org/10.1016/j.plaphy.2021.03.016
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dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	Plant Physiology and Biochemistry (Available online 13 March 2021) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
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repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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Biochemical differences in the skin of two blueberries (Vaccinium corymbosum) varieties with contrasting firmness: implication of ions, metabolites and cell wall related proteins i...

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