Anther Ontogeny and Pollen Development in Southern Highbush Blueberry (Vaccinium corymbosum L.)

Autores
Recalde, José María; Garavello, Miguel Fernando; Alayon Luaces, Paula; González, Ana María
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Southern highbush blueberry (SHB, Vaccinium corymbosum, Ericaceae) enables production in warm, low-chill regions, where breeding success depends on precisely timed pollinations. To support breeding in non-traditional environments, we characterized floral staging, anther wall ontogeny, tubule formation, and pollen development in two SHB cultivars (‘Emerald’, ‘Snowchaser’) grown in commercial orchards. Floral development was divided into seven stages: dormant buds (db), five successive floral-bud stages (botA–botE), and anthesis, based on bud size, corolla exposure and pigmentation, and anther/tubule coloration. Internal events were documented by light, confocal, and scanning electron microscopy. External cues reliably separated stages and tracked male-gametophyte phases: meiosis at botB; callose-encased tetrads at botC; permanent tetrahedral tetrads after callose dissolution at botD; bicellular tetrads from botE to anthesis, released intact via poricidal dehiscence. Anther-wall differentiation followed a consistent sequence and lacked a fibrous, lignified endothecium. We therefore propose a new Ericaceous pattern for blueberry anthers, defined by a transient non-lignified subepidermal stratum. Tubules originated apically as solid outgrowths, hollowed centrifugally to a beveled pore, developed a dorsal supportive zone, and mediated poricidal release of permanent tetrads. No qualitative cultivar differences were detected. The staging framework defines operational windows for pollination, emasculation, and pollen handling in low-chill systems.
EEA Concordia
Fil: Recalde, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Garavello, Miguel Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Alayón Luaces, Paula. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Cátedra de Fruticultura; Argentina.
Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Cátedra de Fruticultura; Argentina.
Fil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: González, Ana María. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentina
Fuente
Horticulturae 11 (11) : 1278. (November 2025)
Materia
Vaccinium corymbosum
Highbush Blueberries
Pollen
Anthers
Biological Development
Arándanos Highbush
Polen
Antera
Desarrollo Biológico
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Anther Ontogeny and Pollen Development in Southern Highbush Blueberry (Vaccinium corymbosum L.)Recalde, José MaríaGaravello, Miguel FernandoAlayon Luaces, PaulaGonzález, Ana MaríaVaccinium corymbosumHighbush BlueberriesPollenAnthersBiological DevelopmentArándanos HighbushPolenAnteraDesarrollo BiológicoSouthern highbush blueberry (SHB, Vaccinium corymbosum, Ericaceae) enables production in warm, low-chill regions, where breeding success depends on precisely timed pollinations. To support breeding in non-traditional environments, we characterized floral staging, anther wall ontogeny, tubule formation, and pollen development in two SHB cultivars (‘Emerald’, ‘Snowchaser’) grown in commercial orchards. Floral development was divided into seven stages: dormant buds (db), five successive floral-bud stages (botA–botE), and anthesis, based on bud size, corolla exposure and pigmentation, and anther/tubule coloration. Internal events were documented by light, confocal, and scanning electron microscopy. External cues reliably separated stages and tracked male-gametophyte phases: meiosis at botB; callose-encased tetrads at botC; permanent tetrahedral tetrads after callose dissolution at botD; bicellular tetrads from botE to anthesis, released intact via poricidal dehiscence. Anther-wall differentiation followed a consistent sequence and lacked a fibrous, lignified endothecium. We therefore propose a new Ericaceous pattern for blueberry anthers, defined by a transient non-lignified subepidermal stratum. Tubules originated apically as solid outgrowths, hollowed centrifugally to a beveled pore, developed a dorsal supportive zone, and mediated poricidal release of permanent tetrads. No qualitative cultivar differences were detected. The staging framework defines operational windows for pollination, emasculation, and pollen handling in low-chill systems.EEA ConcordiaFil: Recalde, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Garavello, Miguel Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Alayón Luaces, Paula. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Cátedra de Fruticultura; Argentina.Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Cátedra de Fruticultura; Argentina.Fil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; ArgentinaFil: González, Ana María. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; ArgentinaMDPI2025-11-06T13:31:25Z2025-11-06T13:31:25Z2025-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24491https://www.mdpi.com/2311-7524/11/11/12782311-7524https://doi.org/10.3390/horticulturae11111278Horticulturae 11 (11) : 1278. (November 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PE-L01-I105, Generación de conocimientos, tecnologías e innovaciones para una fruticultura sostenible adaptadas al riesgo ambiental y a la mecanizacióninfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-11-13T08:48:48Zoai:localhost:20.500.12123/24491instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-13 08:48:48.679INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Anther Ontogeny and Pollen Development in Southern Highbush Blueberry (Vaccinium corymbosum L.)
title Anther Ontogeny and Pollen Development in Southern Highbush Blueberry (Vaccinium corymbosum L.)
spellingShingle Anther Ontogeny and Pollen Development in Southern Highbush Blueberry (Vaccinium corymbosum L.)
Recalde, José María
Vaccinium corymbosum
Highbush Blueberries
Pollen
Anthers
Biological Development
Arándanos Highbush
Polen
Antera
Desarrollo Biológico
title_short Anther Ontogeny and Pollen Development in Southern Highbush Blueberry (Vaccinium corymbosum L.)
title_full Anther Ontogeny and Pollen Development in Southern Highbush Blueberry (Vaccinium corymbosum L.)
title_fullStr Anther Ontogeny and Pollen Development in Southern Highbush Blueberry (Vaccinium corymbosum L.)
title_full_unstemmed Anther Ontogeny and Pollen Development in Southern Highbush Blueberry (Vaccinium corymbosum L.)
title_sort Anther Ontogeny and Pollen Development in Southern Highbush Blueberry (Vaccinium corymbosum L.)
dc.creator.none.fl_str_mv Recalde, José María
Garavello, Miguel Fernando
Alayon Luaces, Paula
González, Ana María
author Recalde, José María
author_facet Recalde, José María
Garavello, Miguel Fernando
Alayon Luaces, Paula
González, Ana María
author_role author
author2 Garavello, Miguel Fernando
Alayon Luaces, Paula
González, Ana María
author2_role author
author
author
dc.subject.none.fl_str_mv Vaccinium corymbosum
Highbush Blueberries
Pollen
Anthers
Biological Development
Arándanos Highbush
Polen
Antera
Desarrollo Biológico
topic Vaccinium corymbosum
Highbush Blueberries
Pollen
Anthers
Biological Development
Arándanos Highbush
Polen
Antera
Desarrollo Biológico
dc.description.none.fl_txt_mv Southern highbush blueberry (SHB, Vaccinium corymbosum, Ericaceae) enables production in warm, low-chill regions, where breeding success depends on precisely timed pollinations. To support breeding in non-traditional environments, we characterized floral staging, anther wall ontogeny, tubule formation, and pollen development in two SHB cultivars (‘Emerald’, ‘Snowchaser’) grown in commercial orchards. Floral development was divided into seven stages: dormant buds (db), five successive floral-bud stages (botA–botE), and anthesis, based on bud size, corolla exposure and pigmentation, and anther/tubule coloration. Internal events were documented by light, confocal, and scanning electron microscopy. External cues reliably separated stages and tracked male-gametophyte phases: meiosis at botB; callose-encased tetrads at botC; permanent tetrahedral tetrads after callose dissolution at botD; bicellular tetrads from botE to anthesis, released intact via poricidal dehiscence. Anther-wall differentiation followed a consistent sequence and lacked a fibrous, lignified endothecium. We therefore propose a new Ericaceous pattern for blueberry anthers, defined by a transient non-lignified subepidermal stratum. Tubules originated apically as solid outgrowths, hollowed centrifugally to a beveled pore, developed a dorsal supportive zone, and mediated poricidal release of permanent tetrads. No qualitative cultivar differences were detected. The staging framework defines operational windows for pollination, emasculation, and pollen handling in low-chill systems.
EEA Concordia
Fil: Recalde, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Garavello, Miguel Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Alayón Luaces, Paula. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Cátedra de Fruticultura; Argentina.
Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Cátedra de Fruticultura; Argentina.
Fil: González, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: González, Ana María. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentina
description Southern highbush blueberry (SHB, Vaccinium corymbosum, Ericaceae) enables production in warm, low-chill regions, where breeding success depends on precisely timed pollinations. To support breeding in non-traditional environments, we characterized floral staging, anther wall ontogeny, tubule formation, and pollen development in two SHB cultivars (‘Emerald’, ‘Snowchaser’) grown in commercial orchards. Floral development was divided into seven stages: dormant buds (db), five successive floral-bud stages (botA–botE), and anthesis, based on bud size, corolla exposure and pigmentation, and anther/tubule coloration. Internal events were documented by light, confocal, and scanning electron microscopy. External cues reliably separated stages and tracked male-gametophyte phases: meiosis at botB; callose-encased tetrads at botC; permanent tetrahedral tetrads after callose dissolution at botD; bicellular tetrads from botE to anthesis, released intact via poricidal dehiscence. Anther-wall differentiation followed a consistent sequence and lacked a fibrous, lignified endothecium. We therefore propose a new Ericaceous pattern for blueberry anthers, defined by a transient non-lignified subepidermal stratum. Tubules originated apically as solid outgrowths, hollowed centrifugally to a beveled pore, developed a dorsal supportive zone, and mediated poricidal release of permanent tetrads. No qualitative cultivar differences were detected. The staging framework defines operational windows for pollination, emasculation, and pollen handling in low-chill systems.
publishDate 2025
dc.date.none.fl_str_mv 2025-11-06T13:31:25Z
2025-11-06T13:31:25Z
2025-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24491
https://www.mdpi.com/2311-7524/11/11/1278
2311-7524
https://doi.org/10.3390/horticulturae11111278
url http://hdl.handle.net/20.500.12123/24491
https://www.mdpi.com/2311-7524/11/11/1278
https://doi.org/10.3390/horticulturae11111278
identifier_str_mv 2311-7524
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PE-L01-I105, Generación de conocimientos, tecnologías e innovaciones para una fruticultura sostenible adaptadas al riesgo ambiental y a la mecanización
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Horticulturae 11 (11) : 1278. (November 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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