Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII

Autores
Vezza, Mariana Elisa; Luna, Dario Fernando; Agostini, Elizabeth; Talano, Melina Andrea
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Despite soybean (Glycine max L.) is often cultivated in areas contaminated with arsenic (As) which causes deleterious effects on its growth, little is known about tolerance mechanisms in response to As exposure. In particular, glutathione (GSH) (γ-glutamyl-cysteinyl-glycine) has antioxidant properties and is precursor of phytochelatin (PCs) synthesis, thus its role in As-treated plants would be relevant. We proposed to assess the GSH role in the response to arsenate (AsV) and arsenite (AsIII) in soybean plants using BSO (L-buthionine-sulfoximine), an inhibitor of its synthesis. BSO treatment was efficient to reduce GSH production in roots and leaves. However, GSH reduction was lower in As/BSO-treated plants compared to control/BSO-treated plants, which suggests an As-induced activation of its synthesis. As a consequence, a decrease in PCs content and PC:As ratio in roots was observed. In addition, a reduction of antioxidant activity, including ascorbate and glutathione peroxidase activity, was detected under BSO treatment, which resulted in an increase of membrane damage and root cell death. Also, low GSH content increased inhibitory effects of As on gas exchange and PSII yield, which could explain the reduction in aerial parts biomass, mainly under AsIII-treatment. BSO-exposure also caused a significant decrease of As accumulation in roots, although increased its translocation to leaves. Our findings suggest that GSH regulated As stress tolerance in soybean plants through complex mechanisms, including redox balance, gas exchange and changes in As accumulation pattern.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Vezza, Mariana Elisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Environmental and experimental botany 162 : 272-282. (June 2019)
Materia
Soja
Glycine Max
Glutatión Reductasa
Arsénico
Fitoquelatos
Antioxidantes
Intercambio de Gases
Soybeans
Glutathione Reductase
Arsenic
Phytochelatins
Antioxidants
Gas Exchange
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4691
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4691
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIIIVezza, Mariana ElisaLuna, Dario FernandoAgostini, ElizabethTalano, Melina AndreaSojaGlycine MaxGlutatión ReductasaArsénicoFitoquelatosAntioxidantesIntercambio de GasesSoybeansGlutathione ReductaseArsenicPhytochelatinsAntioxidantsGas ExchangeDespite soybean (Glycine max L.) is often cultivated in areas contaminated with arsenic (As) which causes deleterious effects on its growth, little is known about tolerance mechanisms in response to As exposure. In particular, glutathione (GSH) (γ-glutamyl-cysteinyl-glycine) has antioxidant properties and is precursor of phytochelatin (PCs) synthesis, thus its role in As-treated plants would be relevant. We proposed to assess the GSH role in the response to arsenate (AsV) and arsenite (AsIII) in soybean plants using BSO (L-buthionine-sulfoximine), an inhibitor of its synthesis. BSO treatment was efficient to reduce GSH production in roots and leaves. However, GSH reduction was lower in As/BSO-treated plants compared to control/BSO-treated plants, which suggests an As-induced activation of its synthesis. As a consequence, a decrease in PCs content and PC:As ratio in roots was observed. In addition, a reduction of antioxidant activity, including ascorbate and glutathione peroxidase activity, was detected under BSO treatment, which resulted in an increase of membrane damage and root cell death. Also, low GSH content increased inhibitory effects of As on gas exchange and PSII yield, which could explain the reduction in aerial parts biomass, mainly under AsIII-treatment. BSO-exposure also caused a significant decrease of As accumulation in roots, although increased its translocation to leaves. Our findings suggest that GSH regulated As stress tolerance in soybean plants through complex mechanisms, including redox balance, gas exchange and changes in As accumulation pattern.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Vezza, Mariana Elisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-03-21T12:52:18Z2019-03-21T12:52:18Z2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4691https://www.sciencedirect.com/science/article/pii/S0098847218317702?via%3Dihub#!0098-8472https://doi.org/10.1016/j.envexpbot.2019.03.002Environmental and experimental botany 162 : 272-282. (June 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:53Zoai:localhost:20.500.12123/4691instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:53.519INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII
title Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII
spellingShingle Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII
Vezza, Mariana Elisa
Soja
Glycine Max
Glutatión Reductasa
Arsénico
Fitoquelatos
Antioxidantes
Intercambio de Gases
Soybeans
Glutathione Reductase
Arsenic
Phytochelatins
Antioxidants
Gas Exchange
title_short Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII
title_full Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII
title_fullStr Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII
title_full_unstemmed Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII
title_sort Glutathione, a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII
dc.creator.none.fl_str_mv Vezza, Mariana Elisa
Luna, Dario Fernando
Agostini, Elizabeth
Talano, Melina Andrea
author Vezza, Mariana Elisa
author_facet Vezza, Mariana Elisa
Luna, Dario Fernando
Agostini, Elizabeth
Talano, Melina Andrea
author_role author
author2 Luna, Dario Fernando
Agostini, Elizabeth
Talano, Melina Andrea
author2_role author
author
author
dc.subject.none.fl_str_mv Soja
Glycine Max
Glutatión Reductasa
Arsénico
Fitoquelatos
Antioxidantes
Intercambio de Gases
Soybeans
Glutathione Reductase
Arsenic
Phytochelatins
Antioxidants
Gas Exchange
topic Soja
Glycine Max
Glutatión Reductasa
Arsénico
Fitoquelatos
Antioxidantes
Intercambio de Gases
Soybeans
Glutathione Reductase
Arsenic
Phytochelatins
Antioxidants
Gas Exchange
dc.description.none.fl_txt_mv Despite soybean (Glycine max L.) is often cultivated in areas contaminated with arsenic (As) which causes deleterious effects on its growth, little is known about tolerance mechanisms in response to As exposure. In particular, glutathione (GSH) (γ-glutamyl-cysteinyl-glycine) has antioxidant properties and is precursor of phytochelatin (PCs) synthesis, thus its role in As-treated plants would be relevant. We proposed to assess the GSH role in the response to arsenate (AsV) and arsenite (AsIII) in soybean plants using BSO (L-buthionine-sulfoximine), an inhibitor of its synthesis. BSO treatment was efficient to reduce GSH production in roots and leaves. However, GSH reduction was lower in As/BSO-treated plants compared to control/BSO-treated plants, which suggests an As-induced activation of its synthesis. As a consequence, a decrease in PCs content and PC:As ratio in roots was observed. In addition, a reduction of antioxidant activity, including ascorbate and glutathione peroxidase activity, was detected under BSO treatment, which resulted in an increase of membrane damage and root cell death. Also, low GSH content increased inhibitory effects of As on gas exchange and PSII yield, which could explain the reduction in aerial parts biomass, mainly under AsIII-treatment. BSO-exposure also caused a significant decrease of As accumulation in roots, although increased its translocation to leaves. Our findings suggest that GSH regulated As stress tolerance in soybean plants through complex mechanisms, including redox balance, gas exchange and changes in As accumulation pattern.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Vezza, Mariana Elisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Despite soybean (Glycine max L.) is often cultivated in areas contaminated with arsenic (As) which causes deleterious effects on its growth, little is known about tolerance mechanisms in response to As exposure. In particular, glutathione (GSH) (γ-glutamyl-cysteinyl-glycine) has antioxidant properties and is precursor of phytochelatin (PCs) synthesis, thus its role in As-treated plants would be relevant. We proposed to assess the GSH role in the response to arsenate (AsV) and arsenite (AsIII) in soybean plants using BSO (L-buthionine-sulfoximine), an inhibitor of its synthesis. BSO treatment was efficient to reduce GSH production in roots and leaves. However, GSH reduction was lower in As/BSO-treated plants compared to control/BSO-treated plants, which suggests an As-induced activation of its synthesis. As a consequence, a decrease in PCs content and PC:As ratio in roots was observed. In addition, a reduction of antioxidant activity, including ascorbate and glutathione peroxidase activity, was detected under BSO treatment, which resulted in an increase of membrane damage and root cell death. Also, low GSH content increased inhibitory effects of As on gas exchange and PSII yield, which could explain the reduction in aerial parts biomass, mainly under AsIII-treatment. BSO-exposure also caused a significant decrease of As accumulation in roots, although increased its translocation to leaves. Our findings suggest that GSH regulated As stress tolerance in soybean plants through complex mechanisms, including redox balance, gas exchange and changes in As accumulation pattern.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-21T12:52:18Z
2019-03-21T12:52:18Z
2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4691
https://www.sciencedirect.com/science/article/pii/S0098847218317702?via%3Dihub#!
0098-8472
https://doi.org/10.1016/j.envexpbot.2019.03.002
url http://hdl.handle.net/20.500.12123/4691
https://www.sciencedirect.com/science/article/pii/S0098847218317702?via%3Dihub#!
https://doi.org/10.1016/j.envexpbot.2019.03.002
identifier_str_mv 0098-8472
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Environmental and experimental botany 162 : 272-282. (June 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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