Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlida...
- Autores
- Crupkin, Andrea Carina; Menone, Mirta Lujan
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The goal of this work was to evaluate the response of the glutathione-S-transferases (GST), glutathione reductase (GR) and catalase (CAT) enzymes in different tissues of the fish Australoheros facetus exposed cadmium (Cd). In liver, a significant decrease of GST activity was observed at 0.031 mg L-1 (p<0.05), while, at higher concentrations, there were non-significant changes compared to controls. Neither the GR nor the CAT activity was different from control. Gills was the most sensitive organ, showing increased GST activity at 0.31 and 1.53 mg L-1 and GR at 3.06 mg L-1; while CAT activity was inhibited at 0.31, 3.06 and 6.13 mg L-1 (p<0.05). The brain GST activity was increased at 1.53 mg L-1 but inhibited at 3.06 and 6.13 mg L-1, meanwhile GR and CAT activities were inhibited at 1.2, 3.06 and 6.13 mg L-1 (p<0.05). The induction of defense enzymes as well as the inhibition of the antioxidant enzyme catalase, even at environmentally relevant concentrations used in this work (e.g. 0.31 mg L-1), denotes the toxic effect that cadmium exerts over the tissues of A. facetus and alerts over the need of doing biomonitoring in areas potentially polluted with metals
El objetivo de este trabajo fue evaluar la respuesta de las enzimas glutatión-S-transferasa (GST), glutatión reductasa (GR) y catalasa (CAT) en diferentes tejidos del pez Australoheros facetus expuesto a cadmio (Cd). En hígado, se observó una disminución de la actividad de GST a 0,031 mg L-1 (p<0,05), mientras que a concentraciones mas elevadas no se hallaron diferencias respecto al control. La branquia fue el órgano mas sensible, presentando un incremento de actividad de GST a 0,31 y 1,53 mg L-1 Cd y de GR a 3,06 mg L-1 Cd; mientras que la actividad de CAT resultó inhibida a 0,31, 3,1 y 6,13 mg L-1 Cd (p<0,05). En el cerebro la actividad de GST se incrementó a 1,53 mg L-1 Cd y se inhibió a 3.1 y 6,13 mg L-1 Cd, mientras que las actividades de GR y CAT se inhibieron a 1,53, 3,1 y 6,13 mg L-1 Cd (p<0,05). La inducción de los sistemas de defensa así como la inhibición del enzima antioxidante catalasa, aún a concentraciones de relevancia ambiental (ejemplo 0,31 mg L-1), demuestra el efecto tóxico que ejerce el Cd en los tejidos de A. facetus y alerta sobre la necesidad de realizar biomonitoreos en áreas potencialmente contaminadas con metales pesados.
Fil: Crupkin, Andrea Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina - Materia
-
biomarkers
cadmium
catalase
glutathione reductase
glutathione-S-transferases - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/25917
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Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces)Cambios en las actividades de glutation-S- transferasa, glutation reductasa y catalasa en Australoheros facetus (Cichlidae, Pisces) expuesto a diferentes concentraciones de cadmioCrupkin, Andrea CarinaMenone, Mirta Lujanbiomarkerscadmiumcatalaseglutathione reductaseglutathione-S-transferaseshttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1The goal of this work was to evaluate the response of the glutathione-S-transferases (GST), glutathione reductase (GR) and catalase (CAT) enzymes in different tissues of the fish Australoheros facetus exposed cadmium (Cd). In liver, a significant decrease of GST activity was observed at 0.031 mg L-1 (p<0.05), while, at higher concentrations, there were non-significant changes compared to controls. Neither the GR nor the CAT activity was different from control. Gills was the most sensitive organ, showing increased GST activity at 0.31 and 1.53 mg L-1 and GR at 3.06 mg L-1; while CAT activity was inhibited at 0.31, 3.06 and 6.13 mg L-1 (p<0.05). The brain GST activity was increased at 1.53 mg L-1 but inhibited at 3.06 and 6.13 mg L-1, meanwhile GR and CAT activities were inhibited at 1.2, 3.06 and 6.13 mg L-1 (p<0.05). The induction of defense enzymes as well as the inhibition of the antioxidant enzyme catalase, even at environmentally relevant concentrations used in this work (e.g. 0.31 mg L-1), denotes the toxic effect that cadmium exerts over the tissues of A. facetus and alerts over the need of doing biomonitoring in areas potentially polluted with metalsEl objetivo de este trabajo fue evaluar la respuesta de las enzimas glutatión-S-transferasa (GST), glutatión reductasa (GR) y catalasa (CAT) en diferentes tejidos del pez Australoheros facetus expuesto a cadmio (Cd). En hígado, se observó una disminución de la actividad de GST a 0,031 mg L-1 (p<0,05), mientras que a concentraciones mas elevadas no se hallaron diferencias respecto al control. La branquia fue el órgano mas sensible, presentando un incremento de actividad de GST a 0,31 y 1,53 mg L-1 Cd y de GR a 3,06 mg L-1 Cd; mientras que la actividad de CAT resultó inhibida a 0,31, 3,1 y 6,13 mg L-1 Cd (p<0,05). En el cerebro la actividad de GST se incrementó a 1,53 mg L-1 Cd y se inhibió a 3.1 y 6,13 mg L-1 Cd, mientras que las actividades de GR y CAT se inhibieron a 1,53, 3,1 y 6,13 mg L-1 Cd (p<0,05). La inducción de los sistemas de defensa así como la inhibición del enzima antioxidante catalasa, aún a concentraciones de relevancia ambiental (ejemplo 0,31 mg L-1), demuestra el efecto tóxico que ejerce el Cd en los tejidos de A. facetus y alerta sobre la necesidad de realizar biomonitoreos en áreas potencialmente contaminadas con metales pesados.Fil: Crupkin, Andrea Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaSociedade Brasileira de Ecotoxicologia2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25917Crupkin, Andrea Carina; Menone, Mirta Lujan; Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces); Sociedade Brasileira de Ecotoxicologia; Ecotoxicology and Environmental Contamination; 8; 1; 1-2013; 21-252317-9643CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5132/eec.2013.01.003info:eu-repo/semantics/altIdentifier/url/https://siaiap32.univali.br/seer/index.php/eec/article/view/3152info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:06Zoai:ri.conicet.gov.ar:11336/25917instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:06.542CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces) Cambios en las actividades de glutation-S- transferasa, glutation reductasa y catalasa en Australoheros facetus (Cichlidae, Pisces) expuesto a diferentes concentraciones de cadmio |
title |
Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces) |
spellingShingle |
Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces) Crupkin, Andrea Carina biomarkers cadmium catalase glutathione reductase glutathione-S-transferases |
title_short |
Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces) |
title_full |
Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces) |
title_fullStr |
Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces) |
title_full_unstemmed |
Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces) |
title_sort |
Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces) |
dc.creator.none.fl_str_mv |
Crupkin, Andrea Carina Menone, Mirta Lujan |
author |
Crupkin, Andrea Carina |
author_facet |
Crupkin, Andrea Carina Menone, Mirta Lujan |
author_role |
author |
author2 |
Menone, Mirta Lujan |
author2_role |
author |
dc.subject.none.fl_str_mv |
biomarkers cadmium catalase glutathione reductase glutathione-S-transferases |
topic |
biomarkers cadmium catalase glutathione reductase glutathione-S-transferases |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.5 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The goal of this work was to evaluate the response of the glutathione-S-transferases (GST), glutathione reductase (GR) and catalase (CAT) enzymes in different tissues of the fish Australoheros facetus exposed cadmium (Cd). In liver, a significant decrease of GST activity was observed at 0.031 mg L-1 (p<0.05), while, at higher concentrations, there were non-significant changes compared to controls. Neither the GR nor the CAT activity was different from control. Gills was the most sensitive organ, showing increased GST activity at 0.31 and 1.53 mg L-1 and GR at 3.06 mg L-1; while CAT activity was inhibited at 0.31, 3.06 and 6.13 mg L-1 (p<0.05). The brain GST activity was increased at 1.53 mg L-1 but inhibited at 3.06 and 6.13 mg L-1, meanwhile GR and CAT activities were inhibited at 1.2, 3.06 and 6.13 mg L-1 (p<0.05). The induction of defense enzymes as well as the inhibition of the antioxidant enzyme catalase, even at environmentally relevant concentrations used in this work (e.g. 0.31 mg L-1), denotes the toxic effect that cadmium exerts over the tissues of A. facetus and alerts over the need of doing biomonitoring in areas potentially polluted with metals El objetivo de este trabajo fue evaluar la respuesta de las enzimas glutatión-S-transferasa (GST), glutatión reductasa (GR) y catalasa (CAT) en diferentes tejidos del pez Australoheros facetus expuesto a cadmio (Cd). En hígado, se observó una disminución de la actividad de GST a 0,031 mg L-1 (p<0,05), mientras que a concentraciones mas elevadas no se hallaron diferencias respecto al control. La branquia fue el órgano mas sensible, presentando un incremento de actividad de GST a 0,31 y 1,53 mg L-1 Cd y de GR a 3,06 mg L-1 Cd; mientras que la actividad de CAT resultó inhibida a 0,31, 3,1 y 6,13 mg L-1 Cd (p<0,05). En el cerebro la actividad de GST se incrementó a 1,53 mg L-1 Cd y se inhibió a 3.1 y 6,13 mg L-1 Cd, mientras que las actividades de GR y CAT se inhibieron a 1,53, 3,1 y 6,13 mg L-1 Cd (p<0,05). La inducción de los sistemas de defensa así como la inhibición del enzima antioxidante catalasa, aún a concentraciones de relevancia ambiental (ejemplo 0,31 mg L-1), demuestra el efecto tóxico que ejerce el Cd en los tejidos de A. facetus y alerta sobre la necesidad de realizar biomonitoreos en áreas potencialmente contaminadas con metales pesados. Fil: Crupkin, Andrea Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina |
description |
The goal of this work was to evaluate the response of the glutathione-S-transferases (GST), glutathione reductase (GR) and catalase (CAT) enzymes in different tissues of the fish Australoheros facetus exposed cadmium (Cd). In liver, a significant decrease of GST activity was observed at 0.031 mg L-1 (p<0.05), while, at higher concentrations, there were non-significant changes compared to controls. Neither the GR nor the CAT activity was different from control. Gills was the most sensitive organ, showing increased GST activity at 0.31 and 1.53 mg L-1 and GR at 3.06 mg L-1; while CAT activity was inhibited at 0.31, 3.06 and 6.13 mg L-1 (p<0.05). The brain GST activity was increased at 1.53 mg L-1 but inhibited at 3.06 and 6.13 mg L-1, meanwhile GR and CAT activities were inhibited at 1.2, 3.06 and 6.13 mg L-1 (p<0.05). The induction of defense enzymes as well as the inhibition of the antioxidant enzyme catalase, even at environmentally relevant concentrations used in this work (e.g. 0.31 mg L-1), denotes the toxic effect that cadmium exerts over the tissues of A. facetus and alerts over the need of doing biomonitoring in areas potentially polluted with metals |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/25917 Crupkin, Andrea Carina; Menone, Mirta Lujan; Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces); Sociedade Brasileira de Ecotoxicologia; Ecotoxicology and Environmental Contamination; 8; 1; 1-2013; 21-25 2317-9643 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/25917 |
identifier_str_mv |
Crupkin, Andrea Carina; Menone, Mirta Lujan; Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces); Sociedade Brasileira de Ecotoxicologia; Ecotoxicology and Environmental Contamination; 8; 1; 1-2013; 21-25 2317-9643 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.5132/eec.2013.01.003 info:eu-repo/semantics/altIdentifier/url/https://siaiap32.univali.br/seer/index.php/eec/article/view/3152 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ecotoxicologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ecotoxicologia |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269892703682560 |
score |
13.13397 |