Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants

Autores
Vezza, Mariana Elisa; Sosa Alderete, Lucas Gastón; Agostini, Elizabeth; Talano, Melina Andrea
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soybean (Glycine max L.) is often cultivated in soils with a high content of arsenic (As). Since this greatly affects crop growth and yield, the response mechanisms underlying tolerance to As should be well understood. Circadian rhythms are known to play a central role in plant adaptation to stressful conditions. Therefore, our aim was to evaluate the effects of arseniate (AsV) and arsenite (AsIII) on the expression of key circadian clock genes (CG) and on the possible diurnal oscillation of physiological events induced by As in soybean plants. Leaves and roots were evaluated during light-dark phases at 4-h intervals. The changes caused by As in the expression profiles of all CGs analyzed depended on its chemical form and the plant organ involved. Overexpression of GmLCL1, GmTOC1, GmPRR9 and GmGI was observed in leaves mainly upon AsV-treatment, while underexpression of GmLCL1 and overexpression of GmPRR9, GmELF4 and GmGI occurred in roots, mainly upon AsIII-treatment. Moreover, As seemed to have an influence on the daily fluctuations observed in events triggered in response to the stress, such as stomatal aperture control, phytochelatin (PC) and glutathione (GSH) content, and total antioxidant, ascorbate (APx) and glutathione peroxidase (GPx) activities. In particular, the increase in the synthesis of PCs (the main As chelators) and the decrease in their precursor, GSH, were higher during the light phase, while APx and GPx activities increased during the dark phase in As-treated plants. The results suggest a link between the circadian clock and the response to As in soybean plants, since exposure to As modified CG expression and induced important changes in the diurnal oscillation of several As-response mechanisms. Circadian clock regulation, then, might play a vital role in the tolerance to As.
Fil: Vezza, Mariana Elisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Materia
ANTIOXIDANT SYSTEM
ARSENIC
CIRCADIAN CLOCK GENES
PHYTOCHELATINS
SOYBEAN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/145196
Acceder
id CONICETDig_4b8f8ddb2f791a68a3e2710ef0448510
oai_identifier_str oai:ri.conicet.gov.ar:11336/145196
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plantsVezza, Mariana ElisaSosa Alderete, Lucas GastónAgostini, ElizabethTalano, Melina AndreaANTIOXIDANT SYSTEMARSENICCIRCADIAN CLOCK GENESPHYTOCHELATINSSOYBEANhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Soybean (Glycine max L.) is often cultivated in soils with a high content of arsenic (As). Since this greatly affects crop growth and yield, the response mechanisms underlying tolerance to As should be well understood. Circadian rhythms are known to play a central role in plant adaptation to stressful conditions. Therefore, our aim was to evaluate the effects of arseniate (AsV) and arsenite (AsIII) on the expression of key circadian clock genes (CG) and on the possible diurnal oscillation of physiological events induced by As in soybean plants. Leaves and roots were evaluated during light-dark phases at 4-h intervals. The changes caused by As in the expression profiles of all CGs analyzed depended on its chemical form and the plant organ involved. Overexpression of GmLCL1, GmTOC1, GmPRR9 and GmGI was observed in leaves mainly upon AsV-treatment, while underexpression of GmLCL1 and overexpression of GmPRR9, GmELF4 and GmGI occurred in roots, mainly upon AsIII-treatment. Moreover, As seemed to have an influence on the daily fluctuations observed in events triggered in response to the stress, such as stomatal aperture control, phytochelatin (PC) and glutathione (GSH) content, and total antioxidant, ascorbate (APx) and glutathione peroxidase (GPx) activities. In particular, the increase in the synthesis of PCs (the main As chelators) and the decrease in their precursor, GSH, were higher during the light phase, while APx and GPx activities increased during the dark phase in As-treated plants. The results suggest a link between the circadian clock and the response to As in soybean plants, since exposure to As modified CG expression and induced important changes in the diurnal oscillation of several As-response mechanisms. Circadian clock regulation, then, might play a vital role in the tolerance to As.Fil: Vezza, Mariana Elisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaPergamon-Elsevier Science Ltd2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/145196Vezza, Mariana Elisa; Sosa Alderete, Lucas Gastón; Agostini, Elizabeth; Talano, Melina Andrea; Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 174; 6-2020; 1-230098-8472CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2020.104054info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0098847220300800info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:57Zoai:ri.conicet.gov.ar:11336/145196instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:57.578CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants
title Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants
spellingShingle Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants
Vezza, Mariana Elisa
ANTIOXIDANT SYSTEM
ARSENIC
CIRCADIAN CLOCK GENES
PHYTOCHELATINS
SOYBEAN
title_short Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants
title_full Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants
title_fullStr Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants
title_full_unstemmed Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants
title_sort Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants
dc.creator.none.fl_str_mv Vezza, Mariana Elisa
Sosa Alderete, Lucas Gastón
Agostini, Elizabeth
Talano, Melina Andrea
author Vezza, Mariana Elisa
author_facet Vezza, Mariana Elisa
Sosa Alderete, Lucas Gastón
Agostini, Elizabeth
Talano, Melina Andrea
author_role author
author2 Sosa Alderete, Lucas Gastón
Agostini, Elizabeth
Talano, Melina Andrea
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANT SYSTEM
ARSENIC
CIRCADIAN CLOCK GENES
PHYTOCHELATINS
SOYBEAN
topic ANTIOXIDANT SYSTEM
ARSENIC
CIRCADIAN CLOCK GENES
PHYTOCHELATINS
SOYBEAN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Soybean (Glycine max L.) is often cultivated in soils with a high content of arsenic (As). Since this greatly affects crop growth and yield, the response mechanisms underlying tolerance to As should be well understood. Circadian rhythms are known to play a central role in plant adaptation to stressful conditions. Therefore, our aim was to evaluate the effects of arseniate (AsV) and arsenite (AsIII) on the expression of key circadian clock genes (CG) and on the possible diurnal oscillation of physiological events induced by As in soybean plants. Leaves and roots were evaluated during light-dark phases at 4-h intervals. The changes caused by As in the expression profiles of all CGs analyzed depended on its chemical form and the plant organ involved. Overexpression of GmLCL1, GmTOC1, GmPRR9 and GmGI was observed in leaves mainly upon AsV-treatment, while underexpression of GmLCL1 and overexpression of GmPRR9, GmELF4 and GmGI occurred in roots, mainly upon AsIII-treatment. Moreover, As seemed to have an influence on the daily fluctuations observed in events triggered in response to the stress, such as stomatal aperture control, phytochelatin (PC) and glutathione (GSH) content, and total antioxidant, ascorbate (APx) and glutathione peroxidase (GPx) activities. In particular, the increase in the synthesis of PCs (the main As chelators) and the decrease in their precursor, GSH, were higher during the light phase, while APx and GPx activities increased during the dark phase in As-treated plants. The results suggest a link between the circadian clock and the response to As in soybean plants, since exposure to As modified CG expression and induced important changes in the diurnal oscillation of several As-response mechanisms. Circadian clock regulation, then, might play a vital role in the tolerance to As.
Fil: Vezza, Mariana Elisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
description Soybean (Glycine max L.) is often cultivated in soils with a high content of arsenic (As). Since this greatly affects crop growth and yield, the response mechanisms underlying tolerance to As should be well understood. Circadian rhythms are known to play a central role in plant adaptation to stressful conditions. Therefore, our aim was to evaluate the effects of arseniate (AsV) and arsenite (AsIII) on the expression of key circadian clock genes (CG) and on the possible diurnal oscillation of physiological events induced by As in soybean plants. Leaves and roots were evaluated during light-dark phases at 4-h intervals. The changes caused by As in the expression profiles of all CGs analyzed depended on its chemical form and the plant organ involved. Overexpression of GmLCL1, GmTOC1, GmPRR9 and GmGI was observed in leaves mainly upon AsV-treatment, while underexpression of GmLCL1 and overexpression of GmPRR9, GmELF4 and GmGI occurred in roots, mainly upon AsIII-treatment. Moreover, As seemed to have an influence on the daily fluctuations observed in events triggered in response to the stress, such as stomatal aperture control, phytochelatin (PC) and glutathione (GSH) content, and total antioxidant, ascorbate (APx) and glutathione peroxidase (GPx) activities. In particular, the increase in the synthesis of PCs (the main As chelators) and the decrease in their precursor, GSH, were higher during the light phase, while APx and GPx activities increased during the dark phase in As-treated plants. The results suggest a link between the circadian clock and the response to As in soybean plants, since exposure to As modified CG expression and induced important changes in the diurnal oscillation of several As-response mechanisms. Circadian clock regulation, then, might play a vital role in the tolerance to As.
publishDate 2020
dc.date.none.fl_str_mv 2020-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/145196
Vezza, Mariana Elisa; Sosa Alderete, Lucas Gastón; Agostini, Elizabeth; Talano, Melina Andrea; Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 174; 6-2020; 1-23
0098-8472
CONICET Digital
CONICET
url http://hdl.handle.net/11336/145196
identifier_str_mv Vezza, Mariana Elisa; Sosa Alderete, Lucas Gastón; Agostini, Elizabeth; Talano, Melina Andrea; Expression of circadian clock genes and diurnal oscillations of key physiological events in response to AsV and AsIII in soybean plants; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 174; 6-2020; 1-23
0098-8472
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2020.104054
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0098847220300800
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268827031699456
score 13.13397

Publicaciones similares