Gut bacteria facilitate adaptation to crop rotation in the western corn rootworm

• Autores: Chu, Chia Ching; Spencer, Joseph L.; Curzi, Matías Joel; Zavala, Jorge Alberto; Seufferheld, Manfredo J.
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Proceedings of the National Academy of Sciences of the United States of America; Vol.110, no.29; 11917-11922; http://www.pnas.org/
• Materia: ANTHROPOGENIC DISTURBANCE; CONTEMPORARY EVOLUTION; DIETARY STRESS; DIGESTIVE ENZYMES; HOST-MICROBE INTERACTION; ANTIBIOTIC AGENT; CYSTEINE PROTEASE INHIBITOR; DNA 16S; ENZYME INHIBITOR; ERYTHROMYCIN; GENTAMICIN; KANAMYCIN; TETRACYCLINE; UNCLASSIFIED DRUG; BEETLE; CONTROLLED STUDY; CROP ROTATION; DIABROTICA VIRWITH VIRGIFERA; DIET; ECOSYSTEM RESTORATION; EVOLUTIONARY ADAPTATION; FIELD CORN; FOLIAGE; HERBIVORY; INSECT; INTESTINE ADAPTATION; INTESTINE FLORA; LANDSCAPE; MAIZE; MICROBIAL COMMUNITY; NONHUMAN; PLAGUE; ROOTWORM; SOYBEAN; UNITED STATES; WILD TYPE; WORM
• Institución: Universidad de Buenos Aires. Facultad de Agronomía

Fil: Chu, Chia Ching. University of Illinois. Department of Crop Sciences. Urbana, IL, USA.
Fil: Spencer, Joseph L. University of Illinois. Illinois Natural History Survey. Champaign, IL, USA.
Fil: Curzi, Matías Joel. University of Illinois. Department of Crop Sciences. Urbana, IL, USA.
Fil: Zavala, Jorge Alberto. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Bioquímica. Buenos Aires, Argentina.
Fil: Zavala, Jorge Alberto. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Zavala, Jorge Alberto. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Zavala, Jorge Alberto. CONICET. Buenos Aires, Argentina.
Fil: Seufferheld, Manfredo J. University of Illinois. Illinois Natural History Survey. Champaign, IL, USA.
Fil: Seufferheld, Manfredo J. University of Illinois. Department of Entomology. Urbana, IL, USA.
Insects are constantly adapting to human-driven landscape changes; however, the roles of their gut microbiota in these processes remain largely unknown. The western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) (Coleoptera: Chrysomelidae) is amajor corn pest that has been controlled via annual rotation between corn (Zea mays) and nonhost soybean (Glycine max) in the United States. This practice selected for a 'rotation-resistant' variant (RR-WCR) with reduced ovipositional fidelity to cornfields.When in soybean fields, RRWCRs also exhibit an elevated tolerance of antiherbivory defenses (i.e., cysteine protease inhibitors) expressed in soybean foliage. Here we show that gut bacterial microbiota is an important factor facilitating this corn specialist's (WCR's) physiological adaptation to brief soybean herbivory. Comparisons of gut microbiota between RR- and wild-type WCR (WT-WCR) revealed concomitant shifts in bacterial community structure with host adaptation to soybean diets. Antibiotic suppression of gut bacteria significantly reduced RR-WCR tolerance of soybean herbivory to the level of WT-WCR, whereas WTWCR were unaffected. Our findings demonstrate that gut bacteria help to facilitate rapid adaptation of insects inmanaged ecosystems.