Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts

Autores
Chu, Chia-Ching; Zavala, Jorge Alberto; Spencer, Joseph L.; Curzi, Matías J.; Fields, Christopher J.; Drnevich, Jenny; Siegfried, Blair D.; Seufferheld, Manfredo Jose
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) is an important pest of corn. Annual crop rotation between corn and soybean disrupts the corn‐dependent WCR life cycle and is widely adopted to manage this pest. This strategy selected for rotation‐resistant (RR) WCR with reduced ovipositional fidelity to corn. Previous studies revealed that RR‐WCR adults exhibit greater tolerance of soybean diets, different gut physiology, and host–microbe interactions compared to rotation‐susceptible wild types (WT). To identify the genetic mechanisms underlying these phenotypic changes, a de novo assembly of the WCR adult gut transcriptome was constructed and used for RNA‐sequencing analyses of RNA libraries from different WCR phenotypes fed with corn or soybean diets. Global gene expression profiles of WT‐ and RR‐WCR were similar when feeding on corn diets, but different when feeding on soybean. Using network‐based methods, we identified gene modules transcriptionally correlated with the RR phenotype. Gene ontology enrichment analyses indicated that the functions of these modules were related to metabolic processes, immune responses, biological adhesion, and other functions/processes that appear to correlate to documented traits in RR populations. These results suggest that gut transcriptomic divergence correlated with brief soybean feeding and other physiological traits may exist between RR‐ and WT‐WCR adults.
Fil: Chu, Chia-Ching. University of Illinois at Urbana; Estados Unidos
Fil: Zavala, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Spencer, Joseph L.. University of Illinois; Estados Unidos
Fil: Curzi, Matías J.. DuPont Pioneer; Argentina
Fil: Fields, Christopher J.. Biotechnology Center; Estados Unidos
Fil: Drnevich, Jenny. Biotechnology Center; Estados Unidos
Fil: Siegfried, Blair D.. Universidad de Nebraska - Lincoln; Estados Unidos
Fil: Seufferheld, Manfredo Jose. University of Illinois at Urbana; Estados Unidos. University of Illinois; Estados Unidos
Materia
Adaptation
Agriculture
Molecular Ecology
Plant Defenses
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46400
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tractsChu, Chia-ChingZavala, Jorge AlbertoSpencer, Joseph L.Curzi, Matías J.Fields, Christopher J.Drnevich, JennySiegfried, Blair D.Seufferheld, Manfredo JoseAdaptationAgricultureMolecular EcologyPlant Defenseshttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) is an important pest of corn. Annual crop rotation between corn and soybean disrupts the corn‐dependent WCR life cycle and is widely adopted to manage this pest. This strategy selected for rotation‐resistant (RR) WCR with reduced ovipositional fidelity to corn. Previous studies revealed that RR‐WCR adults exhibit greater tolerance of soybean diets, different gut physiology, and host–microbe interactions compared to rotation‐susceptible wild types (WT). To identify the genetic mechanisms underlying these phenotypic changes, a de novo assembly of the WCR adult gut transcriptome was constructed and used for RNA‐sequencing analyses of RNA libraries from different WCR phenotypes fed with corn or soybean diets. Global gene expression profiles of WT‐ and RR‐WCR were similar when feeding on corn diets, but different when feeding on soybean. Using network‐based methods, we identified gene modules transcriptionally correlated with the RR phenotype. Gene ontology enrichment analyses indicated that the functions of these modules were related to metabolic processes, immune responses, biological adhesion, and other functions/processes that appear to correlate to documented traits in RR populations. These results suggest that gut transcriptomic divergence correlated with brief soybean feeding and other physiological traits may exist between RR‐ and WT‐WCR adults.Fil: Chu, Chia-Ching. University of Illinois at Urbana; Estados UnidosFil: Zavala, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Spencer, Joseph L.. University of Illinois; Estados UnidosFil: Curzi, Matías J.. DuPont Pioneer; ArgentinaFil: Fields, Christopher J.. Biotechnology Center; Estados UnidosFil: Drnevich, Jenny. Biotechnology Center; Estados UnidosFil: Siegfried, Blair D.. Universidad de Nebraska - Lincoln; Estados UnidosFil: Seufferheld, Manfredo Jose. University of Illinois at Urbana; Estados Unidos. University of Illinois; Estados UnidosWiley Blackwell Publishing, Inc2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46400Chu, Chia-Ching; Zavala, Jorge Alberto; Spencer, Joseph L.; Curzi, Matías J.; Fields, Christopher J.; et al.; Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts; Wiley Blackwell Publishing, Inc; Evolutionary Applications; 8; 7; 7-2015; 692-7041752-4571CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/eva.12278info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/eva.12278info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:58Zoai:ri.conicet.gov.ar:11336/46400instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:59.002CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts
title Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts
spellingShingle Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts
Chu, Chia-Ching
Adaptation
Agriculture
Molecular Ecology
Plant Defenses
title_short Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts
title_full Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts
title_fullStr Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts
title_full_unstemmed Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts
title_sort Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts
dc.creator.none.fl_str_mv Chu, Chia-Ching
Zavala, Jorge Alberto
Spencer, Joseph L.
Curzi, Matías J.
Fields, Christopher J.
Drnevich, Jenny
Siegfried, Blair D.
Seufferheld, Manfredo Jose
author Chu, Chia-Ching
author_facet Chu, Chia-Ching
Zavala, Jorge Alberto
Spencer, Joseph L.
Curzi, Matías J.
Fields, Christopher J.
Drnevich, Jenny
Siegfried, Blair D.
Seufferheld, Manfredo Jose
author_role author
author2 Zavala, Jorge Alberto
Spencer, Joseph L.
Curzi, Matías J.
Fields, Christopher J.
Drnevich, Jenny
Siegfried, Blair D.
Seufferheld, Manfredo Jose
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adaptation
Agriculture
Molecular Ecology
Plant Defenses
topic Adaptation
Agriculture
Molecular Ecology
Plant Defenses
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) is an important pest of corn. Annual crop rotation between corn and soybean disrupts the corn‐dependent WCR life cycle and is widely adopted to manage this pest. This strategy selected for rotation‐resistant (RR) WCR with reduced ovipositional fidelity to corn. Previous studies revealed that RR‐WCR adults exhibit greater tolerance of soybean diets, different gut physiology, and host–microbe interactions compared to rotation‐susceptible wild types (WT). To identify the genetic mechanisms underlying these phenotypic changes, a de novo assembly of the WCR adult gut transcriptome was constructed and used for RNA‐sequencing analyses of RNA libraries from different WCR phenotypes fed with corn or soybean diets. Global gene expression profiles of WT‐ and RR‐WCR were similar when feeding on corn diets, but different when feeding on soybean. Using network‐based methods, we identified gene modules transcriptionally correlated with the RR phenotype. Gene ontology enrichment analyses indicated that the functions of these modules were related to metabolic processes, immune responses, biological adhesion, and other functions/processes that appear to correlate to documented traits in RR populations. These results suggest that gut transcriptomic divergence correlated with brief soybean feeding and other physiological traits may exist between RR‐ and WT‐WCR adults.
Fil: Chu, Chia-Ching. University of Illinois at Urbana; Estados Unidos
Fil: Zavala, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Spencer, Joseph L.. University of Illinois; Estados Unidos
Fil: Curzi, Matías J.. DuPont Pioneer; Argentina
Fil: Fields, Christopher J.. Biotechnology Center; Estados Unidos
Fil: Drnevich, Jenny. Biotechnology Center; Estados Unidos
Fil: Siegfried, Blair D.. Universidad de Nebraska - Lincoln; Estados Unidos
Fil: Seufferheld, Manfredo Jose. University of Illinois at Urbana; Estados Unidos. University of Illinois; Estados Unidos
description The western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) is an important pest of corn. Annual crop rotation between corn and soybean disrupts the corn‐dependent WCR life cycle and is widely adopted to manage this pest. This strategy selected for rotation‐resistant (RR) WCR with reduced ovipositional fidelity to corn. Previous studies revealed that RR‐WCR adults exhibit greater tolerance of soybean diets, different gut physiology, and host–microbe interactions compared to rotation‐susceptible wild types (WT). To identify the genetic mechanisms underlying these phenotypic changes, a de novo assembly of the WCR adult gut transcriptome was constructed and used for RNA‐sequencing analyses of RNA libraries from different WCR phenotypes fed with corn or soybean diets. Global gene expression profiles of WT‐ and RR‐WCR were similar when feeding on corn diets, but different when feeding on soybean. Using network‐based methods, we identified gene modules transcriptionally correlated with the RR phenotype. Gene ontology enrichment analyses indicated that the functions of these modules were related to metabolic processes, immune responses, biological adhesion, and other functions/processes that appear to correlate to documented traits in RR populations. These results suggest that gut transcriptomic divergence correlated with brief soybean feeding and other physiological traits may exist between RR‐ and WT‐WCR adults.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46400
Chu, Chia-Ching; Zavala, Jorge Alberto; Spencer, Joseph L.; Curzi, Matías J.; Fields, Christopher J.; et al.; Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts; Wiley Blackwell Publishing, Inc; Evolutionary Applications; 8; 7; 7-2015; 692-704
1752-4571
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46400
identifier_str_mv Chu, Chia-Ching; Zavala, Jorge Alberto; Spencer, Joseph L.; Curzi, Matías J.; Fields, Christopher J.; et al.; Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts; Wiley Blackwell Publishing, Inc; Evolutionary Applications; 8; 7; 7-2015; 692-704
1752-4571
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/eva.12278
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/eva.12278
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613199194750976
score 13.070432

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