Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA

Autores
Caviedes Vidal, Enrique Juan Raul; Brun, Antonio; Magallanes, MelisA; Barrett Wilt, Gregory A.; Karasov, W. H.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Dietary flexibility in digestive enzyme activity is widespread invertebrates, but mechanisms are poorly understood. Fragmentaryevidence indicates that laboratory rats modulate intestinal•-glucosidase (AG) activity mainly by relying on rapid increase inenzyme transcription followed by translation and translocation to theintestinešs apical, brush border membrane (BBM). We performedthe first unified study of this overall process, relying on activity,transcriptomic and proteomic data from the same animals. We usedas our model nestling house sparrows (Passer domesticus), whichincrease their intestinal AG activity as they switch naturally from lowstarch insect diet to higher starch seed diet. Twenty-four hours after aswitch to a high starch diet, intestinal AG activity and mRNA wereincreased. The protein sucrase-isomaltase (SI), which is responsiblefor all maltase and sucrase activity, was the only hydrolase increasedin the BBM, and its abundance and activity were positivelycorrelated. This is the first demonstration that birds may rely on rapidincrease in enzyme abundance when adjusting to high starch diet.
Fil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Brun, Antonio. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Magallanes, MelisA. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Barrett Wilt, Gregory A.. University of Wisconsin; Estados Unidos
Fil: Karasov, W. H.. University of Wisconsin; Estados Unidos
SICB 2020 Annual Meeting
Austin
Estados Unidos
Society for Integrative and Comparative Biology
Materia
Digestive enzymes
Intestine
mRNA
Passer domesticus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247669
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNACaviedes Vidal, Enrique Juan RaulBrun, AntonioMagallanes, MelisABarrett Wilt, Gregory A.Karasov, W. H.Digestive enzymesIntestinemRNAPasser domesticushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Dietary flexibility in digestive enzyme activity is widespread invertebrates, but mechanisms are poorly understood. Fragmentaryevidence indicates that laboratory rats modulate intestinal•-glucosidase (AG) activity mainly by relying on rapid increase inenzyme transcription followed by translation and translocation to theintestinešs apical, brush border membrane (BBM). We performedthe first unified study of this overall process, relying on activity,transcriptomic and proteomic data from the same animals. We usedas our model nestling house sparrows (Passer domesticus), whichincrease their intestinal AG activity as they switch naturally from lowstarch insect diet to higher starch seed diet. Twenty-four hours after aswitch to a high starch diet, intestinal AG activity and mRNA wereincreased. The protein sucrase-isomaltase (SI), which is responsiblefor all maltase and sucrase activity, was the only hydrolase increasedin the BBM, and its abundance and activity were positivelycorrelated. This is the first demonstration that birds may rely on rapidincrease in enzyme abundance when adjusting to high starch diet.Fil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Brun, Antonio. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Magallanes, MelisA. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Barrett Wilt, Gregory A.. University of Wisconsin; Estados UnidosFil: Karasov, W. H.. University of Wisconsin; Estados UnidosSICB 2020 Annual MeetingAustinEstados UnidosSociety for Integrative and Comparative BiologyThe Society for Integrative and Comparative Biology2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247669Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA; SICB 2020 Annual Meeting; Austin; Estados Unidos; 2020; 59-59CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:40:16Zoai:ri.conicet.gov.ar:11336/247669instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:40:16.387CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA
title Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA
spellingShingle Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA
Caviedes Vidal, Enrique Juan Raul
Digestive enzymes
Intestine
mRNA
Passer domesticus
title_short Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA
title_full Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA
title_fullStr Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA
title_full_unstemmed Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA
title_sort Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA
dc.creator.none.fl_str_mv Caviedes Vidal, Enrique Juan Raul
Brun, Antonio
Magallanes, MelisA
Barrett Wilt, Gregory A.
Karasov, W. H.
author Caviedes Vidal, Enrique Juan Raul
author_facet Caviedes Vidal, Enrique Juan Raul
Brun, Antonio
Magallanes, MelisA
Barrett Wilt, Gregory A.
Karasov, W. H.
author_role author
author2 Brun, Antonio
Magallanes, MelisA
Barrett Wilt, Gregory A.
Karasov, W. H.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Digestive enzymes
Intestine
mRNA
Passer domesticus
topic Digestive enzymes
Intestine
mRNA
Passer domesticus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Dietary flexibility in digestive enzyme activity is widespread invertebrates, but mechanisms are poorly understood. Fragmentaryevidence indicates that laboratory rats modulate intestinal•-glucosidase (AG) activity mainly by relying on rapid increase inenzyme transcription followed by translation and translocation to theintestinešs apical, brush border membrane (BBM). We performedthe first unified study of this overall process, relying on activity,transcriptomic and proteomic data from the same animals. We usedas our model nestling house sparrows (Passer domesticus), whichincrease their intestinal AG activity as they switch naturally from lowstarch insect diet to higher starch seed diet. Twenty-four hours after aswitch to a high starch diet, intestinal AG activity and mRNA wereincreased. The protein sucrase-isomaltase (SI), which is responsiblefor all maltase and sucrase activity, was the only hydrolase increasedin the BBM, and its abundance and activity were positivelycorrelated. This is the first demonstration that birds may rely on rapidincrease in enzyme abundance when adjusting to high starch diet.
Fil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Brun, Antonio. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Magallanes, MelisA. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Barrett Wilt, Gregory A.. University of Wisconsin; Estados Unidos
Fil: Karasov, W. H.. University of Wisconsin; Estados Unidos
SICB 2020 Annual Meeting
Austin
Estados Unidos
Society for Integrative and Comparative Biology
description Dietary flexibility in digestive enzyme activity is widespread invertebrates, but mechanisms are poorly understood. Fragmentaryevidence indicates that laboratory rats modulate intestinal•-glucosidase (AG) activity mainly by relying on rapid increase inenzyme transcription followed by translation and translocation to theintestinešs apical, brush border membrane (BBM). We performedthe first unified study of this overall process, relying on activity,transcriptomic and proteomic data from the same animals. We usedas our model nestling house sparrows (Passer domesticus), whichincrease their intestinal AG activity as they switch naturally from lowstarch insect diet to higher starch seed diet. Twenty-four hours after aswitch to a high starch diet, intestinal AG activity and mRNA wereincreased. The protein sucrase-isomaltase (SI), which is responsiblefor all maltase and sucrase activity, was the only hydrolase increasedin the BBM, and its abundance and activity were positivelycorrelated. This is the first demonstration that birds may rely on rapidincrease in enzyme abundance when adjusting to high starch diet.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247669
Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA; SICB 2020 Annual Meeting; Austin; Estados Unidos; 2020; 59-59
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247669
identifier_str_mv Dietary Adaptation to High Starch Involves Increased Abundance of α-glucosidase and its mRNA; SICB 2020 Annual Meeting; Austin; Estados Unidos; 2020; 59-59
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv The Society for Integrative and Comparative Biology
publisher.none.fl_str_mv The Society for Integrative and Comparative Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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