Hongos micorrízicos aislados de orquídeas terrestres nativas de regiones pristinas en Córdoba (Argentina)

Autores
Fernández Di Pardo, Agustina; Chiocchio, Viviana Mónica; Barrera, Viviana A.; Colombo, Roxana P.; Martínez, Alicia E.; Gasoni, Laura; Godeas, Alicia M.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.
Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.
Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Fernández Di Pardo, Agustina. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Chiocchio, Viviana Mónica. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.
Fil: Chiocchio, Viviana Mónica. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Chiocchio, Viviana Mónica. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Barrera, Viviana A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA). Castelar, Buenos Aires, Argentina.
Fil: Colombo, Roxana P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.
Fil: Martínez, Alicia E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.
Fil: Gasoni, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA). Castelar, Buenos Aires, Argentina.
Fil: Godeas, Alicia M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.
Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Córdoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25°C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained the ITS1-5.8s-ITS4 region that was amplified using primers ITS1 and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the association of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina. Rev. Biol. Trop. 63 (1) : 275-283. Epub 2015 March 01.
Fuente
Revista de Biología Tropical
Vol.63, no.1
275-283
http://www.biologiatropical.ucr.ac.cr/
Materia
RHIZOCTONIA
EPULORHIZA
TERRESTRIAL ORCHIDS
MYCORRHIZA
ITS - RDNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
acceso abierto
Repositorio
FAUBA Digital (UBA-FAUBA)
Institución
Universidad de Buenos Aires. Facultad de Agronomía
OAI Identificador
snrd:2015fernandezdipardo

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oai_identifier_str snrd:2015fernandezdipardo
network_acronym_str FAUBA
repository_id_str 2729
network_name_str FAUBA Digital (UBA-FAUBA)
spelling Hongos micorrízicos aislados de orquídeas terrestres nativas de regiones pristinas en Córdoba (Argentina)Fernández Di Pardo, AgustinaChiocchio, Viviana MónicaBarrera, Viviana A.Colombo, Roxana P.Martínez, Alicia E.Gasoni, LauraGodeas, Alicia M.RHIZOCTONIAEPULORHIZATERRESTRIAL ORCHIDSMYCORRHIZAITS - RDNAFil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.Fil: Fernández Di Pardo, Agustina. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.Fil: Chiocchio, Viviana Mónica. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.Fil: Chiocchio, Viviana Mónica. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.Fil: Chiocchio, Viviana Mónica. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.Fil: Barrera, Viviana A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA). Castelar, Buenos Aires, Argentina.Fil: Colombo, Roxana P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.Fil: Martínez, Alicia E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.Fil: Gasoni, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA). Castelar, Buenos Aires, Argentina.Fil: Godeas, Alicia M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Córdoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25°C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained the ITS1-5.8s-ITS4 region that was amplified using primers ITS1 and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the association of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina. Rev. Biol. Trop. 63 (1) : 275-283. Epub 2015 March 01.2015info:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfissn:0034-7744http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2015fernandezdipardoRevista de Biología TropicalVol.63, no.1275-283http://www.biologiatropical.ucr.ac.cr/reponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaeng1001169Córdoba (province)info:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-09-29T13:41:13Zsnrd:2015fernandezdipardoinstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-09-29 13:41:13.946FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse
dc.title.none.fl_str_mv Hongos micorrízicos aislados de orquídeas terrestres nativas de regiones pristinas en Córdoba (Argentina)
title Hongos micorrízicos aislados de orquídeas terrestres nativas de regiones pristinas en Córdoba (Argentina)
spellingShingle Hongos micorrízicos aislados de orquídeas terrestres nativas de regiones pristinas en Córdoba (Argentina)
Fernández Di Pardo, Agustina
RHIZOCTONIA
EPULORHIZA
TERRESTRIAL ORCHIDS
MYCORRHIZA
ITS - RDNA
title_short Hongos micorrízicos aislados de orquídeas terrestres nativas de regiones pristinas en Córdoba (Argentina)
title_full Hongos micorrízicos aislados de orquídeas terrestres nativas de regiones pristinas en Córdoba (Argentina)
title_fullStr Hongos micorrízicos aislados de orquídeas terrestres nativas de regiones pristinas en Córdoba (Argentina)
title_full_unstemmed Hongos micorrízicos aislados de orquídeas terrestres nativas de regiones pristinas en Córdoba (Argentina)
title_sort Hongos micorrízicos aislados de orquídeas terrestres nativas de regiones pristinas en Córdoba (Argentina)
dc.creator.none.fl_str_mv Fernández Di Pardo, Agustina
Chiocchio, Viviana Mónica
Barrera, Viviana A.
Colombo, Roxana P.
Martínez, Alicia E.
Gasoni, Laura
Godeas, Alicia M.
author Fernández Di Pardo, Agustina
author_facet Fernández Di Pardo, Agustina
Chiocchio, Viviana Mónica
Barrera, Viviana A.
Colombo, Roxana P.
Martínez, Alicia E.
Gasoni, Laura
Godeas, Alicia M.
author_role author
author2 Chiocchio, Viviana Mónica
Barrera, Viviana A.
Colombo, Roxana P.
Martínez, Alicia E.
Gasoni, Laura
Godeas, Alicia M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv RHIZOCTONIA
EPULORHIZA
TERRESTRIAL ORCHIDS
MYCORRHIZA
ITS - RDNA
topic RHIZOCTONIA
EPULORHIZA
TERRESTRIAL ORCHIDS
MYCORRHIZA
ITS - RDNA
dc.description.none.fl_txt_mv Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.
Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.
Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Fernández Di Pardo, Agustina. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Chiocchio, Viviana Mónica. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.
Fil: Chiocchio, Viviana Mónica. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Chiocchio, Viviana Mónica. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Barrera, Viviana A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA). Castelar, Buenos Aires, Argentina.
Fil: Colombo, Roxana P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.
Fil: Martínez, Alicia E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.
Fil: Gasoni, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA). Castelar, Buenos Aires, Argentina.
Fil: Godeas, Alicia M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.
Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Córdoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25°C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained the ITS1-5.8s-ITS4 region that was amplified using primers ITS1 and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the association of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina. Rev. Biol. Trop. 63 (1) : 275-283. Epub 2015 March 01.
description Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Buenos Aires, Argentina.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv issn:0034-7744
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2015fernandezdipardo
identifier_str_mv issn:0034-7744
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2015fernandezdipardo
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
eu_rights_str_mv openAccess
rights_invalid_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv 1001169
Córdoba (province)
dc.source.none.fl_str_mv Revista de Biología Tropical
Vol.63, no.1
275-283
http://www.biologiatropical.ucr.ac.cr/
reponame:FAUBA Digital (UBA-FAUBA)
instname:Universidad de Buenos Aires. Facultad de Agronomía
reponame_str FAUBA Digital (UBA-FAUBA)
collection FAUBA Digital (UBA-FAUBA)
instname_str Universidad de Buenos Aires. Facultad de Agronomía
repository.name.fl_str_mv FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía
repository.mail.fl_str_mv martino@agro.uba.ar;berasa@agro.uba.ar
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