Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)

Autores
Fernández Di Pardo, Agustina; Chiocchio, Viviana M.; Barrera, Viviana Andrea; Colombo, Roxana P.; Martinez, Alicia E.; Gasoni, Amelia Laura; Godeas, Alicia M.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Córdoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25°C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained 1The ITS1-5.8s-ITS4 region that was amplified using primers ITS1 and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the association of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Agronomía. Instituto en Biociencias Agrícolas y Ambientales (INBA). Cátedra Microbiología Agrícola y Ambiental; Argentina
Fil: Fernández Di Pardo, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chiocchio, Viviana M. Universidad de Buenos Aires. Facultad de Agronomía. Instituto en Biociencias Agrícolas y Ambientales (INBA). Cátedra Microbiología Agrícola y Ambiental; Argentina
Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Colombo, Roxana P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Chiocchio, Viviana M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Alicia E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gasoni, Amelia Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Godeas, Alicia M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fuente
Revista de Biología Tropical 63 (1) : 275-283 (marzo 2015)
Materia
Mycorrhizae
Rhizoctonia
Orchidaceae
Cordoba (Argentina)
Epulorhiza
ITS-rDNA
Orquídeas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21482
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spelling Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)Fernández Di Pardo, AgustinaChiocchio, Viviana M.Barrera, Viviana AndreaColombo, Roxana P.Martinez, Alicia E.Gasoni, Amelia LauraGodeas, Alicia M.MycorrhizaeRhizoctoniaOrchidaceaeCordoba (Argentina)EpulorhizaITS-rDNAOrquídeasOrchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Córdoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25°C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained 1The ITS1-5.8s-ITS4 region that was amplified using primers ITS1 and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the association of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Agronomía. Instituto en Biociencias Agrícolas y Ambientales (INBA). Cátedra Microbiología Agrícola y Ambiental; ArgentinaFil: Fernández Di Pardo, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chiocchio, Viviana M. Universidad de Buenos Aires. Facultad de Agronomía. Instituto en Biociencias Agrícolas y Ambientales (INBA). Cátedra Microbiología Agrícola y Ambiental; ArgentinaFil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Colombo, Roxana P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Chiocchio, Viviana M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinez, Alicia E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Gasoni, Amelia Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Godeas, Alicia M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaUniversidad de Costa Rica2025-02-27T10:17:33Z2025-02-27T10:17:33Z2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21482https://revistas.ucr.ac.cr/index.php/rbt/article/view/142260034-77442215-2075https://doi.org/10.15517/rbt.v63i1.14226Revista de Biología Tropical 63 (1) : 275-283 (marzo 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:10Zoai:localhost:20.500.12123/21482instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:10.459INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
title Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
spellingShingle Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
Fernández Di Pardo, Agustina
Mycorrhizae
Rhizoctonia
Orchidaceae
Cordoba (Argentina)
Epulorhiza
ITS-rDNA
Orquídeas
title_short Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
title_full Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
title_fullStr Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
title_full_unstemmed Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
title_sort Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
dc.creator.none.fl_str_mv Fernández Di Pardo, Agustina
Chiocchio, Viviana M.
Barrera, Viviana Andrea
Colombo, Roxana P.
Martinez, Alicia E.
Gasoni, Amelia Laura
Godeas, Alicia M.
author Fernández Di Pardo, Agustina
author_facet Fernández Di Pardo, Agustina
Chiocchio, Viviana M.
Barrera, Viviana Andrea
Colombo, Roxana P.
Martinez, Alicia E.
Gasoni, Amelia Laura
Godeas, Alicia M.
author_role author
author2 Chiocchio, Viviana M.
Barrera, Viviana Andrea
Colombo, Roxana P.
Martinez, Alicia E.
Gasoni, Amelia Laura
Godeas, Alicia M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycorrhizae
Rhizoctonia
Orchidaceae
Cordoba (Argentina)
Epulorhiza
ITS-rDNA
Orquídeas
topic Mycorrhizae
Rhizoctonia
Orchidaceae
Cordoba (Argentina)
Epulorhiza
ITS-rDNA
Orquídeas
dc.description.none.fl_txt_mv Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Córdoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25°C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained 1The ITS1-5.8s-ITS4 region that was amplified using primers ITS1 and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the association of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Agronomía. Instituto en Biociencias Agrícolas y Ambientales (INBA). Cátedra Microbiología Agrícola y Ambiental; Argentina
Fil: Fernández Di Pardo, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chiocchio, Viviana M. Universidad de Buenos Aires. Facultad de Agronomía. Instituto en Biociencias Agrícolas y Ambientales (INBA). Cátedra Microbiología Agrícola y Ambiental; Argentina
Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Colombo, Roxana P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Chiocchio, Viviana M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Alicia E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gasoni, Amelia Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Godeas, Alicia M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
description Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Córdoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25°C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained 1The ITS1-5.8s-ITS4 region that was amplified using primers ITS1 and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the association of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina.
publishDate 2015
dc.date.none.fl_str_mv 2015-03-01
2025-02-27T10:17:33Z
2025-02-27T10:17:33Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21482
https://revistas.ucr.ac.cr/index.php/rbt/article/view/14226
0034-7744
2215-2075
https://doi.org/10.15517/rbt.v63i1.14226
url http://hdl.handle.net/20.500.12123/21482
https://revistas.ucr.ac.cr/index.php/rbt/article/view/14226
https://doi.org/10.15517/rbt.v63i1.14226
identifier_str_mv 0034-7744
2215-2075
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Revista de Biología Tropical 63 (1) : 275-283 (marzo 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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