Prevalence of paratuberculosis in dairy cattle in ecuador
- Autores
- Echeverría, Gustavo; Escobar, Henry; Changoluisa, Darwin; Ron, Lenin; Proanõ, Andrea; Proanõ Pérez, Freddy; Zumárraga, Martín José; De Waard, Jacobus
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis, a chronic infectious contagious disease of the intestinal tract of ruminants that are also associated with Crohn´s disease in humans. The existence of paratuberculosis in Ecuador is virtually unknown; hence, the present study was performed to gain insight into the prevalence of this disease. Methods: Three dairy cattle farms in different geographic regions in Ecuador were investigated for the infection with MAP, and 600 blood samples, 200 of each cattle herd, were processed with an indirect enzyme-linked immunosorbent assay. Fecal samples of the seropositive cows were processed for culture on modified Löwenstein-Jensen medium. Results: One hundred and fifty bovines (25%) resulted seropositive and we confirmed with culture the presence of MAP in 4.7% (7/150) of the seropositive cows. Approximately 20% of the fecal samples of seropositive cows yielded nontuberculous mycobacteria (NTM) species including M. avium subsp. avium, a NTM species closely related to MAP. Conclusions: The seroprevalence of paratuberculosis in this first study for Ecuador is high (25%). We discuss a possible interference of NTM species, isolated from fecal samples, with the diagnosis of paratuberculosis. With this report, a baseline study, we confirm for the first time the presence of paratuberculosis in Ecuador, and we provide the necessary information for future studies and control of this disease.
Fil: Echeverría, Gustavo. Universidad de Buenos Aires; Argentina. Universidad Central del Ecuador; Ecuador
Fil: Escobar, Henry. Universidad Central del Ecuador; Ecuador
Fil: Changoluisa, Darwin. Universidad Central del Ecuador; Ecuador
Fil: Ron, Lenin. Universidad Central del Ecuador; Ecuador
Fil: Proanõ, Andrea. Universidad Central del Ecuador; Ecuador
Fil: Proanõ Pérez, Freddy. Universidad Central del Ecuador; Ecuador
Fil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: De Waard, Jacobus. Universidad Central de Venezuela; Venezuela - Materia
-
ECUADOR
INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
NONTUBERCULOUS MYCOBACTERIA
PARATUBERCULOSIS OR JOHN'S DISEASE
POLYMERASE CHAIN REACTION-INSERTION SEQUENCE 900 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/230466
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/230466 |
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Prevalence of paratuberculosis in dairy cattle in ecuadorEcheverría, GustavoEscobar, HenryChangoluisa, DarwinRon, LeninProanõ, AndreaProanõ Pérez, FreddyZumárraga, Martín JoséDe Waard, JacobusECUADORINDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAYMYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSISNONTUBERCULOUS MYCOBACTERIAPARATUBERCULOSIS OR JOHN'S DISEASEPOLYMERASE CHAIN REACTION-INSERTION SEQUENCE 900https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis, a chronic infectious contagious disease of the intestinal tract of ruminants that are also associated with Crohn´s disease in humans. The existence of paratuberculosis in Ecuador is virtually unknown; hence, the present study was performed to gain insight into the prevalence of this disease. Methods: Three dairy cattle farms in different geographic regions in Ecuador were investigated for the infection with MAP, and 600 blood samples, 200 of each cattle herd, were processed with an indirect enzyme-linked immunosorbent assay. Fecal samples of the seropositive cows were processed for culture on modified Löwenstein-Jensen medium. Results: One hundred and fifty bovines (25%) resulted seropositive and we confirmed with culture the presence of MAP in 4.7% (7/150) of the seropositive cows. Approximately 20% of the fecal samples of seropositive cows yielded nontuberculous mycobacteria (NTM) species including M. avium subsp. avium, a NTM species closely related to MAP. Conclusions: The seroprevalence of paratuberculosis in this first study for Ecuador is high (25%). We discuss a possible interference of NTM species, isolated from fecal samples, with the diagnosis of paratuberculosis. With this report, a baseline study, we confirm for the first time the presence of paratuberculosis in Ecuador, and we provide the necessary information for future studies and control of this disease.Fil: Echeverría, Gustavo. Universidad de Buenos Aires; Argentina. Universidad Central del Ecuador; EcuadorFil: Escobar, Henry. Universidad Central del Ecuador; EcuadorFil: Changoluisa, Darwin. Universidad Central del Ecuador; EcuadorFil: Ron, Lenin. Universidad Central del Ecuador; EcuadorFil: Proanõ, Andrea. Universidad Central del Ecuador; EcuadorFil: Proanõ Pérez, Freddy. Universidad Central del Ecuador; EcuadorFil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: De Waard, Jacobus. Universidad Central de Venezuela; VenezuelaWolters Kluwer Medknow Publications2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230466Echeverría, Gustavo; Escobar, Henry; Changoluisa, Darwin; Ron, Lenin; Proanõ, Andrea; et al.; Prevalence of paratuberculosis in dairy cattle in ecuador; Wolters Kluwer Medknow Publications; International Journal of Mycobacteriology; 9; 1; 1-2020; 1-62212-55312212-554XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4103/ijmy.ijmy_175_19info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:00Zoai:ri.conicet.gov.ar:11336/230466instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:01.042CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Prevalence of paratuberculosis in dairy cattle in ecuador |
title |
Prevalence of paratuberculosis in dairy cattle in ecuador |
spellingShingle |
Prevalence of paratuberculosis in dairy cattle in ecuador Echeverría, Gustavo ECUADOR INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS NONTUBERCULOUS MYCOBACTERIA PARATUBERCULOSIS OR JOHN'S DISEASE POLYMERASE CHAIN REACTION-INSERTION SEQUENCE 900 |
title_short |
Prevalence of paratuberculosis in dairy cattle in ecuador |
title_full |
Prevalence of paratuberculosis in dairy cattle in ecuador |
title_fullStr |
Prevalence of paratuberculosis in dairy cattle in ecuador |
title_full_unstemmed |
Prevalence of paratuberculosis in dairy cattle in ecuador |
title_sort |
Prevalence of paratuberculosis in dairy cattle in ecuador |
dc.creator.none.fl_str_mv |
Echeverría, Gustavo Escobar, Henry Changoluisa, Darwin Ron, Lenin Proanõ, Andrea Proanõ Pérez, Freddy Zumárraga, Martín José De Waard, Jacobus |
author |
Echeverría, Gustavo |
author_facet |
Echeverría, Gustavo Escobar, Henry Changoluisa, Darwin Ron, Lenin Proanõ, Andrea Proanõ Pérez, Freddy Zumárraga, Martín José De Waard, Jacobus |
author_role |
author |
author2 |
Escobar, Henry Changoluisa, Darwin Ron, Lenin Proanõ, Andrea Proanõ Pérez, Freddy Zumárraga, Martín José De Waard, Jacobus |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
ECUADOR INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS NONTUBERCULOUS MYCOBACTERIA PARATUBERCULOSIS OR JOHN'S DISEASE POLYMERASE CHAIN REACTION-INSERTION SEQUENCE 900 |
topic |
ECUADOR INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS NONTUBERCULOUS MYCOBACTERIA PARATUBERCULOSIS OR JOHN'S DISEASE POLYMERASE CHAIN REACTION-INSERTION SEQUENCE 900 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Background: Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis, a chronic infectious contagious disease of the intestinal tract of ruminants that are also associated with Crohn´s disease in humans. The existence of paratuberculosis in Ecuador is virtually unknown; hence, the present study was performed to gain insight into the prevalence of this disease. Methods: Three dairy cattle farms in different geographic regions in Ecuador were investigated for the infection with MAP, and 600 blood samples, 200 of each cattle herd, were processed with an indirect enzyme-linked immunosorbent assay. Fecal samples of the seropositive cows were processed for culture on modified Löwenstein-Jensen medium. Results: One hundred and fifty bovines (25%) resulted seropositive and we confirmed with culture the presence of MAP in 4.7% (7/150) of the seropositive cows. Approximately 20% of the fecal samples of seropositive cows yielded nontuberculous mycobacteria (NTM) species including M. avium subsp. avium, a NTM species closely related to MAP. Conclusions: The seroprevalence of paratuberculosis in this first study for Ecuador is high (25%). We discuss a possible interference of NTM species, isolated from fecal samples, with the diagnosis of paratuberculosis. With this report, a baseline study, we confirm for the first time the presence of paratuberculosis in Ecuador, and we provide the necessary information for future studies and control of this disease. Fil: Echeverría, Gustavo. Universidad de Buenos Aires; Argentina. Universidad Central del Ecuador; Ecuador Fil: Escobar, Henry. Universidad Central del Ecuador; Ecuador Fil: Changoluisa, Darwin. Universidad Central del Ecuador; Ecuador Fil: Ron, Lenin. Universidad Central del Ecuador; Ecuador Fil: Proanõ, Andrea. Universidad Central del Ecuador; Ecuador Fil: Proanõ Pérez, Freddy. Universidad Central del Ecuador; Ecuador Fil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: De Waard, Jacobus. Universidad Central de Venezuela; Venezuela |
description |
Background: Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis, a chronic infectious contagious disease of the intestinal tract of ruminants that are also associated with Crohn´s disease in humans. The existence of paratuberculosis in Ecuador is virtually unknown; hence, the present study was performed to gain insight into the prevalence of this disease. Methods: Three dairy cattle farms in different geographic regions in Ecuador were investigated for the infection with MAP, and 600 blood samples, 200 of each cattle herd, were processed with an indirect enzyme-linked immunosorbent assay. Fecal samples of the seropositive cows were processed for culture on modified Löwenstein-Jensen medium. Results: One hundred and fifty bovines (25%) resulted seropositive and we confirmed with culture the presence of MAP in 4.7% (7/150) of the seropositive cows. Approximately 20% of the fecal samples of seropositive cows yielded nontuberculous mycobacteria (NTM) species including M. avium subsp. avium, a NTM species closely related to MAP. Conclusions: The seroprevalence of paratuberculosis in this first study for Ecuador is high (25%). We discuss a possible interference of NTM species, isolated from fecal samples, with the diagnosis of paratuberculosis. With this report, a baseline study, we confirm for the first time the presence of paratuberculosis in Ecuador, and we provide the necessary information for future studies and control of this disease. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/230466 Echeverría, Gustavo; Escobar, Henry; Changoluisa, Darwin; Ron, Lenin; Proanõ, Andrea; et al.; Prevalence of paratuberculosis in dairy cattle in ecuador; Wolters Kluwer Medknow Publications; International Journal of Mycobacteriology; 9; 1; 1-2020; 1-6 2212-5531 2212-554X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/230466 |
identifier_str_mv |
Echeverría, Gustavo; Escobar, Henry; Changoluisa, Darwin; Ron, Lenin; Proanõ, Andrea; et al.; Prevalence of paratuberculosis in dairy cattle in ecuador; Wolters Kluwer Medknow Publications; International Journal of Mycobacteriology; 9; 1; 1-2020; 1-6 2212-5531 2212-554X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.4103/ijmy.ijmy_175_19 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wolters Kluwer Medknow Publications |
publisher.none.fl_str_mv |
Wolters Kluwer Medknow Publications |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |