Prevalence of paratuberculosis in dairy cattle in ecuador

Autores
Echeverría, Gustavo; Escobar, Henry; Changoluisa, Darwin; Ron, Lenin; Proanõ, Andrea; Proanõ Pérez, Freddy; Zumárraga, Martín José; De Waard, Jacobus
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis, a chronic infectious contagious disease of the intestinal tract of ruminants that are also associated with Crohn´s disease in humans. The existence of paratuberculosis in Ecuador is virtually unknown; hence, the present study was performed to gain insight into the prevalence of this disease. Methods: Three dairy cattle farms in different geographic regions in Ecuador were investigated for the infection with MAP, and 600 blood samples, 200 of each cattle herd, were processed with an indirect enzyme-linked immunosorbent assay. Fecal samples of the seropositive cows were processed for culture on modified Löwenstein-Jensen medium. Results: One hundred and fifty bovines (25%) resulted seropositive and we confirmed with culture the presence of MAP in 4.7% (7/150) of the seropositive cows. Approximately 20% of the fecal samples of seropositive cows yielded nontuberculous mycobacteria (NTM) species including M. avium subsp. avium, a NTM species closely related to MAP. Conclusions: The seroprevalence of paratuberculosis in this first study for Ecuador is high (25%). We discuss a possible interference of NTM species, isolated from fecal samples, with the diagnosis of paratuberculosis. With this report, a baseline study, we confirm for the first time the presence of paratuberculosis in Ecuador, and we provide the necessary information for future studies and control of this disease.
Fil: Echeverría, Gustavo. Universidad de Buenos Aires; Argentina. Universidad Central del Ecuador; Ecuador
Fil: Escobar, Henry. Universidad Central del Ecuador; Ecuador
Fil: Changoluisa, Darwin. Universidad Central del Ecuador; Ecuador
Fil: Ron, Lenin. Universidad Central del Ecuador; Ecuador
Fil: Proanõ, Andrea. Universidad Central del Ecuador; Ecuador
Fil: Proanõ Pérez, Freddy. Universidad Central del Ecuador; Ecuador
Fil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: De Waard, Jacobus. Universidad Central de Venezuela; Venezuela
Materia
ECUADOR
INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
NONTUBERCULOUS MYCOBACTERIA
PARATUBERCULOSIS OR JOHN'S DISEASE
POLYMERASE CHAIN REACTION-INSERTION SEQUENCE 900
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230466

id CONICETDig_ffb6d33cd9ed5a341a73dde7a39b92f9
oai_identifier_str oai:ri.conicet.gov.ar:11336/230466
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prevalence of paratuberculosis in dairy cattle in ecuadorEcheverría, GustavoEscobar, HenryChangoluisa, DarwinRon, LeninProanõ, AndreaProanõ Pérez, FreddyZumárraga, Martín JoséDe Waard, JacobusECUADORINDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAYMYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSISNONTUBERCULOUS MYCOBACTERIAPARATUBERCULOSIS OR JOHN'S DISEASEPOLYMERASE CHAIN REACTION-INSERTION SEQUENCE 900https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis, a chronic infectious contagious disease of the intestinal tract of ruminants that are also associated with Crohn´s disease in humans. The existence of paratuberculosis in Ecuador is virtually unknown; hence, the present study was performed to gain insight into the prevalence of this disease. Methods: Three dairy cattle farms in different geographic regions in Ecuador were investigated for the infection with MAP, and 600 blood samples, 200 of each cattle herd, were processed with an indirect enzyme-linked immunosorbent assay. Fecal samples of the seropositive cows were processed for culture on modified Löwenstein-Jensen medium. Results: One hundred and fifty bovines (25%) resulted seropositive and we confirmed with culture the presence of MAP in 4.7% (7/150) of the seropositive cows. Approximately 20% of the fecal samples of seropositive cows yielded nontuberculous mycobacteria (NTM) species including M. avium subsp. avium, a NTM species closely related to MAP. Conclusions: The seroprevalence of paratuberculosis in this first study for Ecuador is high (25%). We discuss a possible interference of NTM species, isolated from fecal samples, with the diagnosis of paratuberculosis. With this report, a baseline study, we confirm for the first time the presence of paratuberculosis in Ecuador, and we provide the necessary information for future studies and control of this disease.Fil: Echeverría, Gustavo. Universidad de Buenos Aires; Argentina. Universidad Central del Ecuador; EcuadorFil: Escobar, Henry. Universidad Central del Ecuador; EcuadorFil: Changoluisa, Darwin. Universidad Central del Ecuador; EcuadorFil: Ron, Lenin. Universidad Central del Ecuador; EcuadorFil: Proanõ, Andrea. Universidad Central del Ecuador; EcuadorFil: Proanõ Pérez, Freddy. Universidad Central del Ecuador; EcuadorFil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: De Waard, Jacobus. Universidad Central de Venezuela; VenezuelaWolters Kluwer Medknow Publications2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230466Echeverría, Gustavo; Escobar, Henry; Changoluisa, Darwin; Ron, Lenin; Proanõ, Andrea; et al.; Prevalence of paratuberculosis in dairy cattle in ecuador; Wolters Kluwer Medknow Publications; International Journal of Mycobacteriology; 9; 1; 1-2020; 1-62212-55312212-554XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4103/ijmy.ijmy_175_19info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:00Zoai:ri.conicet.gov.ar:11336/230466instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:01.042CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prevalence of paratuberculosis in dairy cattle in ecuador
title Prevalence of paratuberculosis in dairy cattle in ecuador
spellingShingle Prevalence of paratuberculosis in dairy cattle in ecuador
Echeverría, Gustavo
ECUADOR
INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
NONTUBERCULOUS MYCOBACTERIA
PARATUBERCULOSIS OR JOHN'S DISEASE
POLYMERASE CHAIN REACTION-INSERTION SEQUENCE 900
title_short Prevalence of paratuberculosis in dairy cattle in ecuador
title_full Prevalence of paratuberculosis in dairy cattle in ecuador
title_fullStr Prevalence of paratuberculosis in dairy cattle in ecuador
title_full_unstemmed Prevalence of paratuberculosis in dairy cattle in ecuador
title_sort Prevalence of paratuberculosis in dairy cattle in ecuador
dc.creator.none.fl_str_mv Echeverría, Gustavo
Escobar, Henry
Changoluisa, Darwin
Ron, Lenin
Proanõ, Andrea
Proanõ Pérez, Freddy
Zumárraga, Martín José
De Waard, Jacobus
author Echeverría, Gustavo
author_facet Echeverría, Gustavo
Escobar, Henry
Changoluisa, Darwin
Ron, Lenin
Proanõ, Andrea
Proanõ Pérez, Freddy
Zumárraga, Martín José
De Waard, Jacobus
author_role author
author2 Escobar, Henry
Changoluisa, Darwin
Ron, Lenin
Proanõ, Andrea
Proanõ Pérez, Freddy
Zumárraga, Martín José
De Waard, Jacobus
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ECUADOR
INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
NONTUBERCULOUS MYCOBACTERIA
PARATUBERCULOSIS OR JOHN'S DISEASE
POLYMERASE CHAIN REACTION-INSERTION SEQUENCE 900
topic ECUADOR
INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
NONTUBERCULOUS MYCOBACTERIA
PARATUBERCULOSIS OR JOHN'S DISEASE
POLYMERASE CHAIN REACTION-INSERTION SEQUENCE 900
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis, a chronic infectious contagious disease of the intestinal tract of ruminants that are also associated with Crohn´s disease in humans. The existence of paratuberculosis in Ecuador is virtually unknown; hence, the present study was performed to gain insight into the prevalence of this disease. Methods: Three dairy cattle farms in different geographic regions in Ecuador were investigated for the infection with MAP, and 600 blood samples, 200 of each cattle herd, were processed with an indirect enzyme-linked immunosorbent assay. Fecal samples of the seropositive cows were processed for culture on modified Löwenstein-Jensen medium. Results: One hundred and fifty bovines (25%) resulted seropositive and we confirmed with culture the presence of MAP in 4.7% (7/150) of the seropositive cows. Approximately 20% of the fecal samples of seropositive cows yielded nontuberculous mycobacteria (NTM) species including M. avium subsp. avium, a NTM species closely related to MAP. Conclusions: The seroprevalence of paratuberculosis in this first study for Ecuador is high (25%). We discuss a possible interference of NTM species, isolated from fecal samples, with the diagnosis of paratuberculosis. With this report, a baseline study, we confirm for the first time the presence of paratuberculosis in Ecuador, and we provide the necessary information for future studies and control of this disease.
Fil: Echeverría, Gustavo. Universidad de Buenos Aires; Argentina. Universidad Central del Ecuador; Ecuador
Fil: Escobar, Henry. Universidad Central del Ecuador; Ecuador
Fil: Changoluisa, Darwin. Universidad Central del Ecuador; Ecuador
Fil: Ron, Lenin. Universidad Central del Ecuador; Ecuador
Fil: Proanõ, Andrea. Universidad Central del Ecuador; Ecuador
Fil: Proanõ Pérez, Freddy. Universidad Central del Ecuador; Ecuador
Fil: Zumárraga, Martín José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: De Waard, Jacobus. Universidad Central de Venezuela; Venezuela
description Background: Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis, a chronic infectious contagious disease of the intestinal tract of ruminants that are also associated with Crohn´s disease in humans. The existence of paratuberculosis in Ecuador is virtually unknown; hence, the present study was performed to gain insight into the prevalence of this disease. Methods: Three dairy cattle farms in different geographic regions in Ecuador were investigated for the infection with MAP, and 600 blood samples, 200 of each cattle herd, were processed with an indirect enzyme-linked immunosorbent assay. Fecal samples of the seropositive cows were processed for culture on modified Löwenstein-Jensen medium. Results: One hundred and fifty bovines (25%) resulted seropositive and we confirmed with culture the presence of MAP in 4.7% (7/150) of the seropositive cows. Approximately 20% of the fecal samples of seropositive cows yielded nontuberculous mycobacteria (NTM) species including M. avium subsp. avium, a NTM species closely related to MAP. Conclusions: The seroprevalence of paratuberculosis in this first study for Ecuador is high (25%). We discuss a possible interference of NTM species, isolated from fecal samples, with the diagnosis of paratuberculosis. With this report, a baseline study, we confirm for the first time the presence of paratuberculosis in Ecuador, and we provide the necessary information for future studies and control of this disease.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230466
Echeverría, Gustavo; Escobar, Henry; Changoluisa, Darwin; Ron, Lenin; Proanõ, Andrea; et al.; Prevalence of paratuberculosis in dairy cattle in ecuador; Wolters Kluwer Medknow Publications; International Journal of Mycobacteriology; 9; 1; 1-2020; 1-6
2212-5531
2212-554X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230466
identifier_str_mv Echeverría, Gustavo; Escobar, Henry; Changoluisa, Darwin; Ron, Lenin; Proanõ, Andrea; et al.; Prevalence of paratuberculosis in dairy cattle in ecuador; Wolters Kluwer Medknow Publications; International Journal of Mycobacteriology; 9; 1; 1-2020; 1-6
2212-5531
2212-554X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4103/ijmy.ijmy_175_19
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wolters Kluwer Medknow Publications
publisher.none.fl_str_mv Wolters Kluwer Medknow Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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