Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells

Autores
Ortiz, L; Ruiz, Francisco Oscar; Pascual, L; Barberis, Isabel Lucila
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The lactobacilli probiotics maintain a normal vaginal biota and prevent disease recurrence. This microorganisms form a pellicle on the vaginal epithelium that acts as a biologic barrier against colonization by pathogenic bacteria. In this paper were realized assays of exclusion, competition, and displacement. For these test, vaginal epithelial cells, two strains of lactobacilli and pathogenic bacteria (Staphylococcus aureus, Streptococcus agalactiae and Listeria monocytogenes) were used. The lactobacilli strains showed a great capacity of adherence, with a mean of 83.5 ± 26.67 Lactobacillus fermentum cells and 56.2 ± 20.87 Lactobacillus rhamnosus cells per vaginal epithelial cells. L. fermentum and L. rhamnosus were able to reduce the adherence of S. aureus, S. agalactiae and L. monocytogenes in a significant level in this assay (P < 0.01). The lactobacilli used in this study protect the vaginal epithelium through a series of barriers and interference mechanisms. The aim of present study was to assess the ability of vaginal Lactobacillus strains, selected for their probiotic properties, to block the adherence of pathogenic microorganisms in vitro by displacement, competition, and exclusion mechanisms.
Fil: Ortiz, L. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Ruiz, Francisco Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Pascual, L. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Barberis, Isabel Lucila. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Materia
Probiotic
Adherence
Lactobacilli
Listeria Monocytogenes
Streptococcus Agalactiae
Staphylococcus Aureus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33643
Acceder
id CONICETDig_ff7343c728c38868c1a00141223ff0df
oai_identifier_str oai:ri.conicet.gov.ar:11336/33643
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial CellsOrtiz, LRuiz, Francisco OscarPascual, LBarberis, Isabel LucilaProbioticAdherenceLactobacilliListeria MonocytogenesStreptococcus AgalactiaeStaphylococcus Aureushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The lactobacilli probiotics maintain a normal vaginal biota and prevent disease recurrence. This microorganisms form a pellicle on the vaginal epithelium that acts as a biologic barrier against colonization by pathogenic bacteria. In this paper were realized assays of exclusion, competition, and displacement. For these test, vaginal epithelial cells, two strains of lactobacilli and pathogenic bacteria (Staphylococcus aureus, Streptococcus agalactiae and Listeria monocytogenes) were used. The lactobacilli strains showed a great capacity of adherence, with a mean of 83.5 ± 26.67 Lactobacillus fermentum cells and 56.2 ± 20.87 Lactobacillus rhamnosus cells per vaginal epithelial cells. L. fermentum and L. rhamnosus were able to reduce the adherence of S. aureus, S. agalactiae and L. monocytogenes in a significant level in this assay (P < 0.01). The lactobacilli used in this study protect the vaginal epithelium through a series of barriers and interference mechanisms. The aim of present study was to assess the ability of vaginal Lactobacillus strains, selected for their probiotic properties, to block the adherence of pathogenic microorganisms in vitro by displacement, competition, and exclusion mechanisms.Fil: Ortiz, L. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Ruiz, Francisco Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Pascual, L. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Barberis, Isabel Lucila. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaSpringer2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33643Ortiz, L; Ruiz, Francisco Oscar; Pascual, L; Barberis, Isabel Lucila; Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells; Springer; Current Microbiology; 68; 6; 1-2014; 679-6840343-8651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-014-0524-9info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00284-014-0524-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:18:33Zoai:ri.conicet.gov.ar:11336/33643instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:18:34.107CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells
title Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells
spellingShingle Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells
Ortiz, L
Probiotic
Adherence
Lactobacilli
Listeria Monocytogenes
Streptococcus Agalactiae
Staphylococcus Aureus
title_short Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells
title_full Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells
title_fullStr Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells
title_full_unstemmed Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells
title_sort Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells
dc.creator.none.fl_str_mv Ortiz, L
Ruiz, Francisco Oscar
Pascual, L
Barberis, Isabel Lucila
author Ortiz, L
author_facet Ortiz, L
Ruiz, Francisco Oscar
Pascual, L
Barberis, Isabel Lucila
author_role author
author2 Ruiz, Francisco Oscar
Pascual, L
Barberis, Isabel Lucila
author2_role author
author
author
dc.subject.none.fl_str_mv Probiotic
Adherence
Lactobacilli
Listeria Monocytogenes
Streptococcus Agalactiae
Staphylococcus Aureus
topic Probiotic
Adherence
Lactobacilli
Listeria Monocytogenes
Streptococcus Agalactiae
Staphylococcus Aureus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The lactobacilli probiotics maintain a normal vaginal biota and prevent disease recurrence. This microorganisms form a pellicle on the vaginal epithelium that acts as a biologic barrier against colonization by pathogenic bacteria. In this paper were realized assays of exclusion, competition, and displacement. For these test, vaginal epithelial cells, two strains of lactobacilli and pathogenic bacteria (Staphylococcus aureus, Streptococcus agalactiae and Listeria monocytogenes) were used. The lactobacilli strains showed a great capacity of adherence, with a mean of 83.5 ± 26.67 Lactobacillus fermentum cells and 56.2 ± 20.87 Lactobacillus rhamnosus cells per vaginal epithelial cells. L. fermentum and L. rhamnosus were able to reduce the adherence of S. aureus, S. agalactiae and L. monocytogenes in a significant level in this assay (P < 0.01). The lactobacilli used in this study protect the vaginal epithelium through a series of barriers and interference mechanisms. The aim of present study was to assess the ability of vaginal Lactobacillus strains, selected for their probiotic properties, to block the adherence of pathogenic microorganisms in vitro by displacement, competition, and exclusion mechanisms.
Fil: Ortiz, L. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Ruiz, Francisco Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Pascual, L. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Barberis, Isabel Lucila. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
description The lactobacilli probiotics maintain a normal vaginal biota and prevent disease recurrence. This microorganisms form a pellicle on the vaginal epithelium that acts as a biologic barrier against colonization by pathogenic bacteria. In this paper were realized assays of exclusion, competition, and displacement. For these test, vaginal epithelial cells, two strains of lactobacilli and pathogenic bacteria (Staphylococcus aureus, Streptococcus agalactiae and Listeria monocytogenes) were used. The lactobacilli strains showed a great capacity of adherence, with a mean of 83.5 ± 26.67 Lactobacillus fermentum cells and 56.2 ± 20.87 Lactobacillus rhamnosus cells per vaginal epithelial cells. L. fermentum and L. rhamnosus were able to reduce the adherence of S. aureus, S. agalactiae and L. monocytogenes in a significant level in this assay (P < 0.01). The lactobacilli used in this study protect the vaginal epithelium through a series of barriers and interference mechanisms. The aim of present study was to assess the ability of vaginal Lactobacillus strains, selected for their probiotic properties, to block the adherence of pathogenic microorganisms in vitro by displacement, competition, and exclusion mechanisms.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33643
Ortiz, L; Ruiz, Francisco Oscar; Pascual, L; Barberis, Isabel Lucila; Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells; Springer; Current Microbiology; 68; 6; 1-2014; 679-684
0343-8651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33643
identifier_str_mv Ortiz, L; Ruiz, Francisco Oscar; Pascual, L; Barberis, Isabel Lucila; Effect of Two Probiotic Strains of Lactobacillus on In Vitro Adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to Vaginal Epithelial Cells; Springer; Current Microbiology; 68; 6; 1-2014; 679-684
0343-8651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-014-0524-9
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00284-014-0524-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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