Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes

Autores
Londero, Alejandra; Costa, Magdalena; Sucari, Adriana; Leotta, Gerardo Anibal
Año de publicación
2019
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Listeria monocytogenes is a foodborne pathogen. The recent alert for L. monocytogenes in vegetables from Argentina warns about the importance of reinforcing its isolation, characterization and subtyping in food, clinical and environmental samples. The aim of the present study was to compare the discriminatory power of enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), automated ribotyping and pulsed-field gel electrophoresis (PFGE) to subtype strains of L. monocytogenes isolated from Argentine meat and environmental samples. Simpson's Diversity Index (DI) was calculated on the basis of based on the dendrograms obtained in the by cluster analysis, showing the following discriminatory power: ApaI-PFGE (0.980), AscI-PFGE (0.966), ribotyping (0.912), ERIC-PCR (0.886). The ID values between ApaI- and AscI-PFGE and between ribotyping and ERIC-PCR were not significantly different. Of the three techniques evaluated, PFGE showed the highest discriminatory power. However, the subtyping techniques should be accompanied by effective food monitoring strategies and reliable clinical and epidemiological studies.
Fil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Sucari, Adriana. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
DISCRIMINATORY POWER
ERIC
LISTERIA MONOCYTOGENES
PFGE
RIBOTYPING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121440
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/121440
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenesLondero, AlejandraCosta, MagdalenaSucari, AdrianaLeotta, Gerardo AnibalDISCRIMINATORY POWERERICLISTERIA MONOCYTOGENESPFGERIBOTYPINGhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Listeria monocytogenes is a foodborne pathogen. The recent alert for L. monocytogenes in vegetables from Argentina warns about the importance of reinforcing its isolation, characterization and subtyping in food, clinical and environmental samples. The aim of the present study was to compare the discriminatory power of enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), automated ribotyping and pulsed-field gel electrophoresis (PFGE) to subtype strains of L. monocytogenes isolated from Argentine meat and environmental samples. Simpson's Diversity Index (DI) was calculated on the basis of based on the dendrograms obtained in the by cluster analysis, showing the following discriminatory power: ApaI-PFGE (0.980), AscI-PFGE (0.966), ribotyping (0.912), ERIC-PCR (0.886). The ID values between ApaI- and AscI-PFGE and between ribotyping and ERIC-PCR were not significantly different. Of the three techniques evaluated, PFGE showed the highest discriminatory power. However, the subtyping techniques should be accompanied by effective food monitoring strategies and reliable clinical and epidemiological studies.Fil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Sucari, Adriana. División Higiene y Seguridad Alimentaria y Ambiental; ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaAsociación Argentina de Microbiología2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121440Londero, Alejandra; Costa, Magdalena; Sucari, Adriana; Leotta, Gerardo Anibal; Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 51; 4; 10-2019; 359-3621851-7617CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2019.01.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0325754119300045info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:35Zoai:ri.conicet.gov.ar:11336/121440instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:35.604CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes
title Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes
spellingShingle Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes
Londero, Alejandra
DISCRIMINATORY POWER
ERIC
LISTERIA MONOCYTOGENES
PFGE
RIBOTYPING
title_short Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes
title_full Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes
title_fullStr Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes
title_full_unstemmed Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes
title_sort Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes
dc.creator.none.fl_str_mv Londero, Alejandra
Costa, Magdalena
Sucari, Adriana
Leotta, Gerardo Anibal
author Londero, Alejandra
author_facet Londero, Alejandra
Costa, Magdalena
Sucari, Adriana
Leotta, Gerardo Anibal
author_role author
author2 Costa, Magdalena
Sucari, Adriana
Leotta, Gerardo Anibal
author2_role author
author
author
dc.subject.none.fl_str_mv DISCRIMINATORY POWER
ERIC
LISTERIA MONOCYTOGENES
PFGE
RIBOTYPING
topic DISCRIMINATORY POWER
ERIC
LISTERIA MONOCYTOGENES
PFGE
RIBOTYPING
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Listeria monocytogenes is a foodborne pathogen. The recent alert for L. monocytogenes in vegetables from Argentina warns about the importance of reinforcing its isolation, characterization and subtyping in food, clinical and environmental samples. The aim of the present study was to compare the discriminatory power of enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), automated ribotyping and pulsed-field gel electrophoresis (PFGE) to subtype strains of L. monocytogenes isolated from Argentine meat and environmental samples. Simpson's Diversity Index (DI) was calculated on the basis of based on the dendrograms obtained in the by cluster analysis, showing the following discriminatory power: ApaI-PFGE (0.980), AscI-PFGE (0.966), ribotyping (0.912), ERIC-PCR (0.886). The ID values between ApaI- and AscI-PFGE and between ribotyping and ERIC-PCR were not significantly different. Of the three techniques evaluated, PFGE showed the highest discriminatory power. However, the subtyping techniques should be accompanied by effective food monitoring strategies and reliable clinical and epidemiological studies.
Fil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Sucari, Adriana. División Higiene y Seguridad Alimentaria y Ambiental; Argentina
Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description Listeria monocytogenes is a foodborne pathogen. The recent alert for L. monocytogenes in vegetables from Argentina warns about the importance of reinforcing its isolation, characterization and subtyping in food, clinical and environmental samples. The aim of the present study was to compare the discriminatory power of enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), automated ribotyping and pulsed-field gel electrophoresis (PFGE) to subtype strains of L. monocytogenes isolated from Argentine meat and environmental samples. Simpson's Diversity Index (DI) was calculated on the basis of based on the dendrograms obtained in the by cluster analysis, showing the following discriminatory power: ApaI-PFGE (0.980), AscI-PFGE (0.966), ribotyping (0.912), ERIC-PCR (0.886). The ID values between ApaI- and AscI-PFGE and between ribotyping and ERIC-PCR were not significantly different. Of the three techniques evaluated, PFGE showed the highest discriminatory power. However, the subtyping techniques should be accompanied by effective food monitoring strategies and reliable clinical and epidemiological studies.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121440
Londero, Alejandra; Costa, Magdalena; Sucari, Adriana; Leotta, Gerardo Anibal; Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 51; 4; 10-2019; 359-362
1851-7617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121440
identifier_str_mv Londero, Alejandra; Costa, Magdalena; Sucari, Adriana; Leotta, Gerardo Anibal; Comparison of three molecular subtyping techniques for Listeria monocytogenes Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 51; 4; 10-2019; 359-362
1851-7617
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2019.01.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0325754119300045
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asociación Argentina de Microbiología
publisher.none.fl_str_mv Asociación Argentina de Microbiología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268611989733376
score 13.13397

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