Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione
- Autores
- Carpentieri, Agata Rita; Marchionatti, Ana María; Areco, V.; del Valle Perez, Adriana; Centeno, Viviana Andrea; Tolosa, Nori Graciela
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The intestinal Ca2+ absorption is inhibited by menadione (MEN) through oxidative stress and apoptosis. The aim of this study was to elucidate whether the antioxidant and antiapoptotic properties of melatonin (MEL) could protect the gut against the oxidant MEN. For this purpose, 4-week-old chicks were divided into four groups: (1) controls, (2) treated i.p. with MEN (2.5 lmol/kg of b.w.), (3) treated i.p. with MEL (10 mg/kg of b.w.), and (4) treated with 10 mg MEL/kg of b.w after 2.5 lmol MEN/kg of b.w. Oxidative stress was assessed by determination of glutathione (GSH) and protein carbonyl contents as well as antioxidant enzyme activities. Apoptosis was assayed by the TUNEL technique, protein expression, and activity of caspase 3. The data show that MEL restores the intestinal Ca2+ absorption altered by MEN. In addition, MEL reversed the effects caused by MEN such as decrease in GSH levels, increase in the carbonyl content, alteration in mitochondrial membrane permeability, and enhancement of superoxide dismutase and catalase activities. Apoptosis triggered by MEN in the intestinal cells was arrested by MEL, as indicated by normalization of the mitochondrial membrane permeability, caspase 3 activity, and DNA fragmentation. In conclusion, MEL reverses the inhibition of intestinal Ca 2+ absorption produced by MEN counteracting oxidative stress and apoptosis. These findings suggest that MEL could be a potential drug of choice for the reversal of impaired intestinal Ca 2+ absorption in certain gut disorders that occur with oxidative stress and apoptosis.
Fil: Carpentieri, Agata Rita. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Odontologia; Argentina
Fil: Marchionatti, Ana María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Areco, V.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: del Valle Perez, Adriana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Centeno, Viviana Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Odontologia; Argentina
Fil: Tolosa, Nori Graciela. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Catedra de Bioquimica y Biologia Molecular; Argentina - Materia
-
Melatonin
Calcium Absorption
Apoptosis
Oxidative Stress
Menadione - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/11454
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Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadioneCarpentieri, Agata RitaMarchionatti, Ana MaríaAreco, V.del Valle Perez, AdrianaCenteno, Viviana AndreaTolosa, Nori GracielaMelatoninCalcium AbsorptionApoptosisOxidative StressMenadionehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The intestinal Ca2+ absorption is inhibited by menadione (MEN) through oxidative stress and apoptosis. The aim of this study was to elucidate whether the antioxidant and antiapoptotic properties of melatonin (MEL) could protect the gut against the oxidant MEN. For this purpose, 4-week-old chicks were divided into four groups: (1) controls, (2) treated i.p. with MEN (2.5 lmol/kg of b.w.), (3) treated i.p. with MEL (10 mg/kg of b.w.), and (4) treated with 10 mg MEL/kg of b.w after 2.5 lmol MEN/kg of b.w. Oxidative stress was assessed by determination of glutathione (GSH) and protein carbonyl contents as well as antioxidant enzyme activities. Apoptosis was assayed by the TUNEL technique, protein expression, and activity of caspase 3. The data show that MEL restores the intestinal Ca2+ absorption altered by MEN. In addition, MEL reversed the effects caused by MEN such as decrease in GSH levels, increase in the carbonyl content, alteration in mitochondrial membrane permeability, and enhancement of superoxide dismutase and catalase activities. Apoptosis triggered by MEN in the intestinal cells was arrested by MEL, as indicated by normalization of the mitochondrial membrane permeability, caspase 3 activity, and DNA fragmentation. In conclusion, MEL reverses the inhibition of intestinal Ca 2+ absorption produced by MEN counteracting oxidative stress and apoptosis. These findings suggest that MEL could be a potential drug of choice for the reversal of impaired intestinal Ca 2+ absorption in certain gut disorders that occur with oxidative stress and apoptosis.Fil: Carpentieri, Agata Rita. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Odontologia; ArgentinaFil: Marchionatti, Ana María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Areco, V.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: del Valle Perez, Adriana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Centeno, Viviana Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Odontologia; ArgentinaFil: Tolosa, Nori Graciela. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Catedra de Bioquimica y Biologia Molecular; ArgentinaSpringer2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11454Carpentieri, Agata Rita; Marchionatti, Ana María; Areco, V.; del Valle Perez, Adriana; Centeno, Viviana Andrea; et al.; Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione; Springer; Molecular And Cellular Biochemistry; 387; 1; 2-2014; 197-2050300-81771573-4919enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11010-013-1885-2info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s11010-013-1885-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:42Zoai:ri.conicet.gov.ar:11336/11454instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:42.702CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione |
title |
Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione |
spellingShingle |
Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione Carpentieri, Agata Rita Melatonin Calcium Absorption Apoptosis Oxidative Stress Menadione |
title_short |
Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione |
title_full |
Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione |
title_fullStr |
Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione |
title_full_unstemmed |
Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione |
title_sort |
Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione |
dc.creator.none.fl_str_mv |
Carpentieri, Agata Rita Marchionatti, Ana María Areco, V. del Valle Perez, Adriana Centeno, Viviana Andrea Tolosa, Nori Graciela |
author |
Carpentieri, Agata Rita |
author_facet |
Carpentieri, Agata Rita Marchionatti, Ana María Areco, V. del Valle Perez, Adriana Centeno, Viviana Andrea Tolosa, Nori Graciela |
author_role |
author |
author2 |
Marchionatti, Ana María Areco, V. del Valle Perez, Adriana Centeno, Viviana Andrea Tolosa, Nori Graciela |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Melatonin Calcium Absorption Apoptosis Oxidative Stress Menadione |
topic |
Melatonin Calcium Absorption Apoptosis Oxidative Stress Menadione |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The intestinal Ca2+ absorption is inhibited by menadione (MEN) through oxidative stress and apoptosis. The aim of this study was to elucidate whether the antioxidant and antiapoptotic properties of melatonin (MEL) could protect the gut against the oxidant MEN. For this purpose, 4-week-old chicks were divided into four groups: (1) controls, (2) treated i.p. with MEN (2.5 lmol/kg of b.w.), (3) treated i.p. with MEL (10 mg/kg of b.w.), and (4) treated with 10 mg MEL/kg of b.w after 2.5 lmol MEN/kg of b.w. Oxidative stress was assessed by determination of glutathione (GSH) and protein carbonyl contents as well as antioxidant enzyme activities. Apoptosis was assayed by the TUNEL technique, protein expression, and activity of caspase 3. The data show that MEL restores the intestinal Ca2+ absorption altered by MEN. In addition, MEL reversed the effects caused by MEN such as decrease in GSH levels, increase in the carbonyl content, alteration in mitochondrial membrane permeability, and enhancement of superoxide dismutase and catalase activities. Apoptosis triggered by MEN in the intestinal cells was arrested by MEL, as indicated by normalization of the mitochondrial membrane permeability, caspase 3 activity, and DNA fragmentation. In conclusion, MEL reverses the inhibition of intestinal Ca 2+ absorption produced by MEN counteracting oxidative stress and apoptosis. These findings suggest that MEL could be a potential drug of choice for the reversal of impaired intestinal Ca 2+ absorption in certain gut disorders that occur with oxidative stress and apoptosis. Fil: Carpentieri, Agata Rita. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Odontologia; Argentina Fil: Marchionatti, Ana María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: Areco, V.. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: del Valle Perez, Adriana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: Centeno, Viviana Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Odontologia; Argentina Fil: Tolosa, Nori Graciela. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Catedra de Bioquimica y Biologia Molecular; Argentina |
description |
The intestinal Ca2+ absorption is inhibited by menadione (MEN) through oxidative stress and apoptosis. The aim of this study was to elucidate whether the antioxidant and antiapoptotic properties of melatonin (MEL) could protect the gut against the oxidant MEN. For this purpose, 4-week-old chicks were divided into four groups: (1) controls, (2) treated i.p. with MEN (2.5 lmol/kg of b.w.), (3) treated i.p. with MEL (10 mg/kg of b.w.), and (4) treated with 10 mg MEL/kg of b.w after 2.5 lmol MEN/kg of b.w. Oxidative stress was assessed by determination of glutathione (GSH) and protein carbonyl contents as well as antioxidant enzyme activities. Apoptosis was assayed by the TUNEL technique, protein expression, and activity of caspase 3. The data show that MEL restores the intestinal Ca2+ absorption altered by MEN. In addition, MEL reversed the effects caused by MEN such as decrease in GSH levels, increase in the carbonyl content, alteration in mitochondrial membrane permeability, and enhancement of superoxide dismutase and catalase activities. Apoptosis triggered by MEN in the intestinal cells was arrested by MEL, as indicated by normalization of the mitochondrial membrane permeability, caspase 3 activity, and DNA fragmentation. In conclusion, MEL reverses the inhibition of intestinal Ca 2+ absorption produced by MEN counteracting oxidative stress and apoptosis. These findings suggest that MEL could be a potential drug of choice for the reversal of impaired intestinal Ca 2+ absorption in certain gut disorders that occur with oxidative stress and apoptosis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/11454 Carpentieri, Agata Rita; Marchionatti, Ana María; Areco, V.; del Valle Perez, Adriana; Centeno, Viviana Andrea; et al.; Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione; Springer; Molecular And Cellular Biochemistry; 387; 1; 2-2014; 197-205 0300-8177 1573-4919 |
url |
http://hdl.handle.net/11336/11454 |
identifier_str_mv |
Carpentieri, Agata Rita; Marchionatti, Ana María; Areco, V.; del Valle Perez, Adriana; Centeno, Viviana Andrea; et al.; Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione; Springer; Molecular And Cellular Biochemistry; 387; 1; 2-2014; 197-205 0300-8177 1573-4919 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11010-013-1885-2 info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s11010-013-1885-2 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269654616113152 |
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13.13397 |