Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy
- Autores
- Servetto, Natalia; Cremonezzi, David César; Simes, Juan Carlos; Moya, Mónica; Soriano, Fernando; Palma, Jose Atilio; Campana, Vilma
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The objective of the present work was to study the effect of helium–neon (He–Ne) and gallium–arsenide (Ga–As) laser upon inflammatory biomarkers associated with oxidative stress: fibrinogen, nitric oxide (NO), L-citrulline, and superoxide dismutase (SOD). These were evaluated through histological assessment, in rats with experimental myopathy. Materials and Methods: The groups studied were: (A) control, (B) injured, (C) injured and treated with He–Ne laser, (D) injured and treated with Ga–As laser, (E) irradiated with He–Ne; and (F) irradiated with Ga–As laser. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left posterior limb muscle at the same point on 5 consecutive days, in groups B, C, and D. Low-level laser therapy (LLLT) was applied with 9.5 J/cm2 daily for 7 consecutive days with each laser. The determination of the biomarkers was made by spectrophotometry. The muscles (5/8, single blinded) were stained with Gomori Trichrome and examined by optic microscopy. The quantitative variables were statistically analyzed by the Fisher's test and categorical data by the Axionvision 4.8 program. Pearson's chi-squared test was applied, setting significant difference at P < 0.05 for all cases. Results: In group B, the biomarkers were significantly increased compared to the other groups (P < 0.001), except for NO which in group B decreased significantly (P < 0.001). In group B, there was a higher inflammatory infiltration level (80.67%) in relation to destroyed fibers. Conclusions: LLLT caused significant changes in inflammatory biomarkers and oxidative stress: decreased levels of fibrinogen, L-citrulline and SOD as opposed to the increase of NO in rats with experimental myopathies and significant muscle recovery.
Fil: Servetto, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Cremonezzi, David César. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Nacional de Clínicas; Argentina
Fil: Simes, Juan Carlos. Universidad Nacional de Córdoba; Argentina
Fil: Moya, Mónica. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina
Fil: Soriano, Fernando. Instituto Argentino de Medicina Laser; Argentina
Fil: Palma, Jose Atilio. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina
Fil: Campana, Vilma. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina. Instituto Argentino de Medicina Laser; Argentina - Materia
-
HELIUM-NEON
GALLIUM-ARSENIDE
BIOMARKERS
OXIDATIVE STRESS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/280948
Ver los metadatos del registro completo
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Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathyServetto, NataliaCremonezzi, David CésarSimes, Juan CarlosMoya, MónicaSoriano, FernandoPalma, Jose AtilioCampana, VilmaHELIUM-NEONGALLIUM-ARSENIDEBIOMARKERSOXIDATIVE STRESShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The objective of the present work was to study the effect of helium–neon (He–Ne) and gallium–arsenide (Ga–As) laser upon inflammatory biomarkers associated with oxidative stress: fibrinogen, nitric oxide (NO), L-citrulline, and superoxide dismutase (SOD). These were evaluated through histological assessment, in rats with experimental myopathy. Materials and Methods: The groups studied were: (A) control, (B) injured, (C) injured and treated with He–Ne laser, (D) injured and treated with Ga–As laser, (E) irradiated with He–Ne; and (F) irradiated with Ga–As laser. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left posterior limb muscle at the same point on 5 consecutive days, in groups B, C, and D. Low-level laser therapy (LLLT) was applied with 9.5 J/cm2 daily for 7 consecutive days with each laser. The determination of the biomarkers was made by spectrophotometry. The muscles (5/8, single blinded) were stained with Gomori Trichrome and examined by optic microscopy. The quantitative variables were statistically analyzed by the Fisher's test and categorical data by the Axionvision 4.8 program. Pearson's chi-squared test was applied, setting significant difference at P < 0.05 for all cases. Results: In group B, the biomarkers were significantly increased compared to the other groups (P < 0.001), except for NO which in group B decreased significantly (P < 0.001). In group B, there was a higher inflammatory infiltration level (80.67%) in relation to destroyed fibers. Conclusions: LLLT caused significant changes in inflammatory biomarkers and oxidative stress: decreased levels of fibrinogen, L-citrulline and SOD as opposed to the increase of NO in rats with experimental myopathies and significant muscle recovery.Fil: Servetto, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Cremonezzi, David César. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Nacional de Clínicas; ArgentinaFil: Simes, Juan Carlos. Universidad Nacional de Córdoba; ArgentinaFil: Moya, Mónica. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; ArgentinaFil: Soriano, Fernando. Instituto Argentino de Medicina Laser; ArgentinaFil: Palma, Jose Atilio. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; ArgentinaFil: Campana, Vilma. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina. Instituto Argentino de Medicina Laser; ArgentinaWiley-liss, div John Wiley & Sons Inc.2010-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280948Servetto, Natalia; Cremonezzi, David César; Simes, Juan Carlos; Moya, Mónica; Soriano, Fernando; et al.; Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy; Wiley-liss, div John Wiley & Sons Inc.; Lasers In Surgery And Medicine; 42; 6; 7-2010; 577-5830196-8092CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/lsm.20910info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/lsm.20910info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T12:20:38Zoai:ri.conicet.gov.ar:11336/280948instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 12:20:38.419CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy |
| title |
Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy |
| spellingShingle |
Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy Servetto, Natalia HELIUM-NEON GALLIUM-ARSENIDE BIOMARKERS OXIDATIVE STRESS |
| title_short |
Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy |
| title_full |
Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy |
| title_fullStr |
Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy |
| title_full_unstemmed |
Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy |
| title_sort |
Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy |
| dc.creator.none.fl_str_mv |
Servetto, Natalia Cremonezzi, David César Simes, Juan Carlos Moya, Mónica Soriano, Fernando Palma, Jose Atilio Campana, Vilma |
| author |
Servetto, Natalia |
| author_facet |
Servetto, Natalia Cremonezzi, David César Simes, Juan Carlos Moya, Mónica Soriano, Fernando Palma, Jose Atilio Campana, Vilma |
| author_role |
author |
| author2 |
Cremonezzi, David César Simes, Juan Carlos Moya, Mónica Soriano, Fernando Palma, Jose Atilio Campana, Vilma |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
HELIUM-NEON GALLIUM-ARSENIDE BIOMARKERS OXIDATIVE STRESS |
| topic |
HELIUM-NEON GALLIUM-ARSENIDE BIOMARKERS OXIDATIVE STRESS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
The objective of the present work was to study the effect of helium–neon (He–Ne) and gallium–arsenide (Ga–As) laser upon inflammatory biomarkers associated with oxidative stress: fibrinogen, nitric oxide (NO), L-citrulline, and superoxide dismutase (SOD). These were evaluated through histological assessment, in rats with experimental myopathy. Materials and Methods: The groups studied were: (A) control, (B) injured, (C) injured and treated with He–Ne laser, (D) injured and treated with Ga–As laser, (E) irradiated with He–Ne; and (F) irradiated with Ga–As laser. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left posterior limb muscle at the same point on 5 consecutive days, in groups B, C, and D. Low-level laser therapy (LLLT) was applied with 9.5 J/cm2 daily for 7 consecutive days with each laser. The determination of the biomarkers was made by spectrophotometry. The muscles (5/8, single blinded) were stained with Gomori Trichrome and examined by optic microscopy. The quantitative variables were statistically analyzed by the Fisher's test and categorical data by the Axionvision 4.8 program. Pearson's chi-squared test was applied, setting significant difference at P < 0.05 for all cases. Results: In group B, the biomarkers were significantly increased compared to the other groups (P < 0.001), except for NO which in group B decreased significantly (P < 0.001). In group B, there was a higher inflammatory infiltration level (80.67%) in relation to destroyed fibers. Conclusions: LLLT caused significant changes in inflammatory biomarkers and oxidative stress: decreased levels of fibrinogen, L-citrulline and SOD as opposed to the increase of NO in rats with experimental myopathies and significant muscle recovery. Fil: Servetto, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina Fil: Cremonezzi, David César. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Nacional de Clínicas; Argentina Fil: Simes, Juan Carlos. Universidad Nacional de Córdoba; Argentina Fil: Moya, Mónica. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina Fil: Soriano, Fernando. Instituto Argentino de Medicina Laser; Argentina Fil: Palma, Jose Atilio. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina Fil: Campana, Vilma. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina. Instituto Argentino de Medicina Laser; Argentina |
| description |
The objective of the present work was to study the effect of helium–neon (He–Ne) and gallium–arsenide (Ga–As) laser upon inflammatory biomarkers associated with oxidative stress: fibrinogen, nitric oxide (NO), L-citrulline, and superoxide dismutase (SOD). These were evaluated through histological assessment, in rats with experimental myopathy. Materials and Methods: The groups studied were: (A) control, (B) injured, (C) injured and treated with He–Ne laser, (D) injured and treated with Ga–As laser, (E) irradiated with He–Ne; and (F) irradiated with Ga–As laser. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left posterior limb muscle at the same point on 5 consecutive days, in groups B, C, and D. Low-level laser therapy (LLLT) was applied with 9.5 J/cm2 daily for 7 consecutive days with each laser. The determination of the biomarkers was made by spectrophotometry. The muscles (5/8, single blinded) were stained with Gomori Trichrome and examined by optic microscopy. The quantitative variables were statistically analyzed by the Fisher's test and categorical data by the Axionvision 4.8 program. Pearson's chi-squared test was applied, setting significant difference at P < 0.05 for all cases. Results: In group B, the biomarkers were significantly increased compared to the other groups (P < 0.001), except for NO which in group B decreased significantly (P < 0.001). In group B, there was a higher inflammatory infiltration level (80.67%) in relation to destroyed fibers. Conclusions: LLLT caused significant changes in inflammatory biomarkers and oxidative stress: decreased levels of fibrinogen, L-citrulline and SOD as opposed to the increase of NO in rats with experimental myopathies and significant muscle recovery. |
| publishDate |
2010 |
| dc.date.none.fl_str_mv |
2010-07 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/280948 Servetto, Natalia; Cremonezzi, David César; Simes, Juan Carlos; Moya, Mónica; Soriano, Fernando; et al.; Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy; Wiley-liss, div John Wiley & Sons Inc.; Lasers In Surgery And Medicine; 42; 6; 7-2010; 577-583 0196-8092 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/280948 |
| identifier_str_mv |
Servetto, Natalia; Cremonezzi, David César; Simes, Juan Carlos; Moya, Mónica; Soriano, Fernando; et al.; Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy; Wiley-liss, div John Wiley & Sons Inc.; Lasers In Surgery And Medicine; 42; 6; 7-2010; 577-583 0196-8092 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1002/lsm.20910 info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/lsm.20910 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley-liss, div John Wiley & Sons Inc. |
| publisher.none.fl_str_mv |
Wiley-liss, div John Wiley & Sons Inc. |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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