Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy

Autores
Servetto, Natalia; Cremonezzi, David César; Simes, Juan Carlos; Moya, Mónica; Soriano, Fernando; Palma, Jose Atilio; Campana, Vilma
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of the present work was to study the effect of helium–neon (He–Ne) and gallium–arsenide (Ga–As) laser upon inflammatory biomarkers associated with oxidative stress: fibrinogen, nitric oxide (NO), L-citrulline, and superoxide dismutase (SOD). These were evaluated through histological assessment, in rats with experimental myopathy. Materials and Methods: The groups studied were: (A) control, (B) injured, (C) injured and treated with He–Ne laser, (D) injured and treated with Ga–As laser, (E) irradiated with He–Ne; and (F) irradiated with Ga–As laser. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left posterior limb muscle at the same point on 5 consecutive days, in groups B, C, and D. Low-level laser therapy (LLLT) was applied with 9.5 J/cm2 daily for 7 consecutive days with each laser. The determination of the biomarkers was made by spectrophotometry. The muscles (5/8, single blinded) were stained with Gomori Trichrome and examined by optic microscopy. The quantitative variables were statistically analyzed by the Fisher's test and categorical data by the Axionvision 4.8 program. Pearson's chi-squared test was applied, setting significant difference at P < 0.05 for all cases. Results: In group B, the biomarkers were significantly increased compared to the other groups (P < 0.001), except for NO which in group B decreased significantly (P < 0.001). In group B, there was a higher inflammatory infiltration level (80.67%) in relation to destroyed fibers. Conclusions: LLLT caused significant changes in inflammatory biomarkers and oxidative stress: decreased levels of fibrinogen, L-citrulline and SOD as opposed to the increase of NO in rats with experimental myopathies and significant muscle recovery.
Fil: Servetto, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Cremonezzi, David César. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Nacional de Clínicas; Argentina
Fil: Simes, Juan Carlos. Universidad Nacional de Córdoba; Argentina
Fil: Moya, Mónica. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina
Fil: Soriano, Fernando. Instituto Argentino de Medicina Laser; Argentina
Fil: Palma, Jose Atilio. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina
Fil: Campana, Vilma. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina. Instituto Argentino de Medicina Laser; Argentina
Materia
HELIUM-NEON
GALLIUM-ARSENIDE
BIOMARKERS
OXIDATIVE STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280948

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oai_identifier_str oai:ri.conicet.gov.ar:11336/280948
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathyServetto, NataliaCremonezzi, David CésarSimes, Juan CarlosMoya, MónicaSoriano, FernandoPalma, Jose AtilioCampana, VilmaHELIUM-NEONGALLIUM-ARSENIDEBIOMARKERSOXIDATIVE STRESShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The objective of the present work was to study the effect of helium–neon (He–Ne) and gallium–arsenide (Ga–As) laser upon inflammatory biomarkers associated with oxidative stress: fibrinogen, nitric oxide (NO), L-citrulline, and superoxide dismutase (SOD). These were evaluated through histological assessment, in rats with experimental myopathy. Materials and Methods: The groups studied were: (A) control, (B) injured, (C) injured and treated with He–Ne laser, (D) injured and treated with Ga–As laser, (E) irradiated with He–Ne; and (F) irradiated with Ga–As laser. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left posterior limb muscle at the same point on 5 consecutive days, in groups B, C, and D. Low-level laser therapy (LLLT) was applied with 9.5 J/cm2 daily for 7 consecutive days with each laser. The determination of the biomarkers was made by spectrophotometry. The muscles (5/8, single blinded) were stained with Gomori Trichrome and examined by optic microscopy. The quantitative variables were statistically analyzed by the Fisher's test and categorical data by the Axionvision 4.8 program. Pearson's chi-squared test was applied, setting significant difference at P < 0.05 for all cases. Results: In group B, the biomarkers were significantly increased compared to the other groups (P < 0.001), except for NO which in group B decreased significantly (P < 0.001). In group B, there was a higher inflammatory infiltration level (80.67%) in relation to destroyed fibers. Conclusions: LLLT caused significant changes in inflammatory biomarkers and oxidative stress: decreased levels of fibrinogen, L-citrulline and SOD as opposed to the increase of NO in rats with experimental myopathies and significant muscle recovery.Fil: Servetto, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Cremonezzi, David César. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Nacional de Clínicas; ArgentinaFil: Simes, Juan Carlos. Universidad Nacional de Córdoba; ArgentinaFil: Moya, Mónica. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; ArgentinaFil: Soriano, Fernando. Instituto Argentino de Medicina Laser; ArgentinaFil: Palma, Jose Atilio. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; ArgentinaFil: Campana, Vilma. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina. Instituto Argentino de Medicina Laser; ArgentinaWiley-liss, div John Wiley & Sons Inc.2010-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280948Servetto, Natalia; Cremonezzi, David César; Simes, Juan Carlos; Moya, Mónica; Soriano, Fernando; et al.; Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy; Wiley-liss, div John Wiley & Sons Inc.; Lasers In Surgery And Medicine; 42; 6; 7-2010; 577-5830196-8092CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/lsm.20910info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/lsm.20910info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T12:20:38Zoai:ri.conicet.gov.ar:11336/280948instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 12:20:38.419CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy
title Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy
spellingShingle Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy
Servetto, Natalia
HELIUM-NEON
GALLIUM-ARSENIDE
BIOMARKERS
OXIDATIVE STRESS
title_short Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy
title_full Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy
title_fullStr Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy
title_full_unstemmed Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy
title_sort Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy
dc.creator.none.fl_str_mv Servetto, Natalia
Cremonezzi, David César
Simes, Juan Carlos
Moya, Mónica
Soriano, Fernando
Palma, Jose Atilio
Campana, Vilma
author Servetto, Natalia
author_facet Servetto, Natalia
Cremonezzi, David César
Simes, Juan Carlos
Moya, Mónica
Soriano, Fernando
Palma, Jose Atilio
Campana, Vilma
author_role author
author2 Cremonezzi, David César
Simes, Juan Carlos
Moya, Mónica
Soriano, Fernando
Palma, Jose Atilio
Campana, Vilma
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv HELIUM-NEON
GALLIUM-ARSENIDE
BIOMARKERS
OXIDATIVE STRESS
topic HELIUM-NEON
GALLIUM-ARSENIDE
BIOMARKERS
OXIDATIVE STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The objective of the present work was to study the effect of helium–neon (He–Ne) and gallium–arsenide (Ga–As) laser upon inflammatory biomarkers associated with oxidative stress: fibrinogen, nitric oxide (NO), L-citrulline, and superoxide dismutase (SOD). These were evaluated through histological assessment, in rats with experimental myopathy. Materials and Methods: The groups studied were: (A) control, (B) injured, (C) injured and treated with He–Ne laser, (D) injured and treated with Ga–As laser, (E) irradiated with He–Ne; and (F) irradiated with Ga–As laser. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left posterior limb muscle at the same point on 5 consecutive days, in groups B, C, and D. Low-level laser therapy (LLLT) was applied with 9.5 J/cm2 daily for 7 consecutive days with each laser. The determination of the biomarkers was made by spectrophotometry. The muscles (5/8, single blinded) were stained with Gomori Trichrome and examined by optic microscopy. The quantitative variables were statistically analyzed by the Fisher's test and categorical data by the Axionvision 4.8 program. Pearson's chi-squared test was applied, setting significant difference at P < 0.05 for all cases. Results: In group B, the biomarkers were significantly increased compared to the other groups (P < 0.001), except for NO which in group B decreased significantly (P < 0.001). In group B, there was a higher inflammatory infiltration level (80.67%) in relation to destroyed fibers. Conclusions: LLLT caused significant changes in inflammatory biomarkers and oxidative stress: decreased levels of fibrinogen, L-citrulline and SOD as opposed to the increase of NO in rats with experimental myopathies and significant muscle recovery.
Fil: Servetto, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Cremonezzi, David César. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Hospital Nacional de Clínicas; Argentina
Fil: Simes, Juan Carlos. Universidad Nacional de Córdoba; Argentina
Fil: Moya, Mónica. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina
Fil: Soriano, Fernando. Instituto Argentino de Medicina Laser; Argentina
Fil: Palma, Jose Atilio. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina
Fil: Campana, Vilma. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de La Rioja; Argentina. Instituto Argentino de Medicina Laser; Argentina
description The objective of the present work was to study the effect of helium–neon (He–Ne) and gallium–arsenide (Ga–As) laser upon inflammatory biomarkers associated with oxidative stress: fibrinogen, nitric oxide (NO), L-citrulline, and superoxide dismutase (SOD). These were evaluated through histological assessment, in rats with experimental myopathy. Materials and Methods: The groups studied were: (A) control, (B) injured, (C) injured and treated with He–Ne laser, (D) injured and treated with Ga–As laser, (E) irradiated with He–Ne; and (F) irradiated with Ga–As laser. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left posterior limb muscle at the same point on 5 consecutive days, in groups B, C, and D. Low-level laser therapy (LLLT) was applied with 9.5 J/cm2 daily for 7 consecutive days with each laser. The determination of the biomarkers was made by spectrophotometry. The muscles (5/8, single blinded) were stained with Gomori Trichrome and examined by optic microscopy. The quantitative variables were statistically analyzed by the Fisher's test and categorical data by the Axionvision 4.8 program. Pearson's chi-squared test was applied, setting significant difference at P < 0.05 for all cases. Results: In group B, the biomarkers were significantly increased compared to the other groups (P < 0.001), except for NO which in group B decreased significantly (P < 0.001). In group B, there was a higher inflammatory infiltration level (80.67%) in relation to destroyed fibers. Conclusions: LLLT caused significant changes in inflammatory biomarkers and oxidative stress: decreased levels of fibrinogen, L-citrulline and SOD as opposed to the increase of NO in rats with experimental myopathies and significant muscle recovery.
publishDate 2010
dc.date.none.fl_str_mv 2010-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280948
Servetto, Natalia; Cremonezzi, David César; Simes, Juan Carlos; Moya, Mónica; Soriano, Fernando; et al.; Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy; Wiley-liss, div John Wiley & Sons Inc.; Lasers In Surgery And Medicine; 42; 6; 7-2010; 577-583
0196-8092
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280948
identifier_str_mv Servetto, Natalia; Cremonezzi, David César; Simes, Juan Carlos; Moya, Mónica; Soriano, Fernando; et al.; Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low‐level laser therapy in experimental myopathy; Wiley-liss, div John Wiley & Sons Inc.; Lasers In Surgery And Medicine; 42; 6; 7-2010; 577-583
0196-8092
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/lsm.20910
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/lsm.20910
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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