Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats

Autores
Vignola, María Belén; Dávila, Soledad; Cremonezzi, David; Simes, Juan Carlos; Palma, José A.; Campana, Vilma
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of pulsed electromagnetic field (PEMF) therapy, also called magnetic therapy, upon inflammatory biomarkers associated with oxidative stress plasma fibrinogen, nitric oxide (NO), L-citrulline, carbonyl groups, and superoxide dismutase (SOD) was evaluated through histological assessment, in rats with experimental myopathy. The groups studied were: (A) control (intact rats that received PEMF sham exposures); (B) rats with myopathy and sacrificed 24 h later; (C) rats with myopathy; (D) rats with myopathy and treated with PEMF; and (E) intact rats treated with PEMF. Groups A, C, D, and E were sacrificed 8 days later. Myopathy was induced by injecting 50 ml of 1% carrageenan l (type IV) once subplantar. Treatment was carried out with PEMF emitting equipment with two flat solenoid disks for 8 consecutive days in groups D and E, at 20 mT and 50 Hz for 30 min/day/rat. The biomarkers were determined by spectrophotometry. The muscles (5/8) were stained with Hematoxylin-Eosin and examined by optic microscopy. Quantitative variables were statistically analyzed by the Fisher test, and categorical applying Pearson?s Chi Squared test at p , 0.05 for all cases. In Groups B and C, the biomarkers were significantly increased compared to A, D, and E groups: fibrinogen (p , 0.001); NO, L-citrulline and carbonyl groups (p , 0.05); SOD (p , 0.01) as well as the percentage of area with inflammatory infiltration (p , 0.001). PEMF caused decreased levels of fibrinogen, L-citrulline, NO, SOD, and carbonyl groups and significant muscle recovery in rats with experimental myopathies.
Fil: Vignola, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Dávila, Soledad. Universidad Nacional de La Rioja; Argentina
Fil: Cremonezzi, David. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Simes, Juan Carlos. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina
Fil: Palma, José A.. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina
Fil: Campana, Vilma. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina. Universidad Nacional de La Rioja; Argentina
Materia
Experimental Myopathy
Inflammatory Biomarkers
Magnetotherapy
Oxidative Stress
Pemf
Pulsed Electromagnetic Field Therapy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84092
Acceder
id CONICETDig_8639c6453d739bb373efd2c174ad9f0b
oai_identifier_str oai:ri.conicet.gov.ar:11336/84092
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in ratsVignola, María BelénDávila, SoledadCremonezzi, DavidSimes, Juan CarlosPalma, José A.Campana, VilmaExperimental MyopathyInflammatory BiomarkersMagnetotherapyOxidative StressPemfPulsed Electromagnetic Field Therapyhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The effect of pulsed electromagnetic field (PEMF) therapy, also called magnetic therapy, upon inflammatory biomarkers associated with oxidative stress plasma fibrinogen, nitric oxide (NO), L-citrulline, carbonyl groups, and superoxide dismutase (SOD) was evaluated through histological assessment, in rats with experimental myopathy. The groups studied were: (A) control (intact rats that received PEMF sham exposures); (B) rats with myopathy and sacrificed 24 h later; (C) rats with myopathy; (D) rats with myopathy and treated with PEMF; and (E) intact rats treated with PEMF. Groups A, C, D, and E were sacrificed 8 days later. Myopathy was induced by injecting 50 ml of 1% carrageenan l (type IV) once subplantar. Treatment was carried out with PEMF emitting equipment with two flat solenoid disks for 8 consecutive days in groups D and E, at 20 mT and 50 Hz for 30 min/day/rat. The biomarkers were determined by spectrophotometry. The muscles (5/8) were stained with Hematoxylin-Eosin and examined by optic microscopy. Quantitative variables were statistically analyzed by the Fisher test, and categorical applying Pearson?s Chi Squared test at p , 0.05 for all cases. In Groups B and C, the biomarkers were significantly increased compared to A, D, and E groups: fibrinogen (p , 0.001); NO, L-citrulline and carbonyl groups (p , 0.05); SOD (p , 0.01) as well as the percentage of area with inflammatory infiltration (p , 0.001). PEMF caused decreased levels of fibrinogen, L-citrulline, NO, SOD, and carbonyl groups and significant muscle recovery in rats with experimental myopathies.Fil: Vignola, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Dávila, Soledad. Universidad Nacional de La Rioja; ArgentinaFil: Cremonezzi, David. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba; ArgentinaFil: Simes, Juan Carlos. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; ArgentinaFil: Palma, José A.. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; ArgentinaFil: Campana, Vilma. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina. Universidad Nacional de La Rioja; ArgentinaInforma Healthcare2012-12-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84092Vignola, María Belén; Dávila, Soledad; Cremonezzi, David; Simes, Juan Carlos; Palma, José A.; et al.; Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats; Informa Healthcare; Electromagnetic Biology And Medicine; 31; 4; 19-12-2012; 320-3321536-83781536-8386CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3109/15368378.2011.641706info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/15368378.2011.641706info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:03:25Zoai:ri.conicet.gov.ar:11336/84092instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:03:25.672CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats
title Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats
spellingShingle Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats
Vignola, María Belén
Experimental Myopathy
Inflammatory Biomarkers
Magnetotherapy
Oxidative Stress
Pemf
Pulsed Electromagnetic Field Therapy
title_short Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats
title_full Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats
title_fullStr Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats
title_full_unstemmed Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats
title_sort Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats
dc.creator.none.fl_str_mv Vignola, María Belén
Dávila, Soledad
Cremonezzi, David
Simes, Juan Carlos
Palma, José A.
Campana, Vilma
author Vignola, María Belén
author_facet Vignola, María Belén
Dávila, Soledad
Cremonezzi, David
Simes, Juan Carlos
Palma, José A.
Campana, Vilma
author_role author
author2 Dávila, Soledad
Cremonezzi, David
Simes, Juan Carlos
Palma, José A.
Campana, Vilma
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Experimental Myopathy
Inflammatory Biomarkers
Magnetotherapy
Oxidative Stress
Pemf
Pulsed Electromagnetic Field Therapy
topic Experimental Myopathy
Inflammatory Biomarkers
Magnetotherapy
Oxidative Stress
Pemf
Pulsed Electromagnetic Field Therapy
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The effect of pulsed electromagnetic field (PEMF) therapy, also called magnetic therapy, upon inflammatory biomarkers associated with oxidative stress plasma fibrinogen, nitric oxide (NO), L-citrulline, carbonyl groups, and superoxide dismutase (SOD) was evaluated through histological assessment, in rats with experimental myopathy. The groups studied were: (A) control (intact rats that received PEMF sham exposures); (B) rats with myopathy and sacrificed 24 h later; (C) rats with myopathy; (D) rats with myopathy and treated with PEMF; and (E) intact rats treated with PEMF. Groups A, C, D, and E were sacrificed 8 days later. Myopathy was induced by injecting 50 ml of 1% carrageenan l (type IV) once subplantar. Treatment was carried out with PEMF emitting equipment with two flat solenoid disks for 8 consecutive days in groups D and E, at 20 mT and 50 Hz for 30 min/day/rat. The biomarkers were determined by spectrophotometry. The muscles (5/8) were stained with Hematoxylin-Eosin and examined by optic microscopy. Quantitative variables were statistically analyzed by the Fisher test, and categorical applying Pearson?s Chi Squared test at p , 0.05 for all cases. In Groups B and C, the biomarkers were significantly increased compared to A, D, and E groups: fibrinogen (p , 0.001); NO, L-citrulline and carbonyl groups (p , 0.05); SOD (p , 0.01) as well as the percentage of area with inflammatory infiltration (p , 0.001). PEMF caused decreased levels of fibrinogen, L-citrulline, NO, SOD, and carbonyl groups and significant muscle recovery in rats with experimental myopathies.
Fil: Vignola, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Dávila, Soledad. Universidad Nacional de La Rioja; Argentina
Fil: Cremonezzi, David. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Simes, Juan Carlos. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina
Fil: Palma, José A.. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina
Fil: Campana, Vilma. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina. Universidad Nacional de La Rioja; Argentina
description The effect of pulsed electromagnetic field (PEMF) therapy, also called magnetic therapy, upon inflammatory biomarkers associated with oxidative stress plasma fibrinogen, nitric oxide (NO), L-citrulline, carbonyl groups, and superoxide dismutase (SOD) was evaluated through histological assessment, in rats with experimental myopathy. The groups studied were: (A) control (intact rats that received PEMF sham exposures); (B) rats with myopathy and sacrificed 24 h later; (C) rats with myopathy; (D) rats with myopathy and treated with PEMF; and (E) intact rats treated with PEMF. Groups A, C, D, and E were sacrificed 8 days later. Myopathy was induced by injecting 50 ml of 1% carrageenan l (type IV) once subplantar. Treatment was carried out with PEMF emitting equipment with two flat solenoid disks for 8 consecutive days in groups D and E, at 20 mT and 50 Hz for 30 min/day/rat. The biomarkers were determined by spectrophotometry. The muscles (5/8) were stained with Hematoxylin-Eosin and examined by optic microscopy. Quantitative variables were statistically analyzed by the Fisher test, and categorical applying Pearson?s Chi Squared test at p , 0.05 for all cases. In Groups B and C, the biomarkers were significantly increased compared to A, D, and E groups: fibrinogen (p , 0.001); NO, L-citrulline and carbonyl groups (p , 0.05); SOD (p , 0.01) as well as the percentage of area with inflammatory infiltration (p , 0.001). PEMF caused decreased levels of fibrinogen, L-citrulline, NO, SOD, and carbonyl groups and significant muscle recovery in rats with experimental myopathies.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84092
Vignola, María Belén; Dávila, Soledad; Cremonezzi, David; Simes, Juan Carlos; Palma, José A.; et al.; Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats; Informa Healthcare; Electromagnetic Biology And Medicine; 31; 4; 19-12-2012; 320-332
1536-8378
1536-8386
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84092
identifier_str_mv Vignola, María Belén; Dávila, Soledad; Cremonezzi, David; Simes, Juan Carlos; Palma, José A.; et al.; Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats; Informa Healthcare; Electromagnetic Biology And Medicine; 31; 4; 19-12-2012; 320-332
1536-8378
1536-8386
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3109/15368378.2011.641706
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/15368378.2011.641706
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Informa Healthcare
publisher.none.fl_str_mv Informa Healthcare
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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