Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile

Autores
Prabhakaran, Molamma P.; Zamani, Maedeh; Felice, Betiana; Ramakrishna, Seeram
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Advanced engineering of materials for the development of drug delivery devices provides scope for novel and versatile strategies for treatment of various diseases. 'Electrospraying' was used to prepare PLGA microparticles and further encapsulate the drug, metronidazole (Met) within the particles to function as a drug delivery system. Two different solvents were utilized for the preparation of drug loaded PLGA particles, whereby the polymeric solution in dichloromethane (DCM) produced particles of bigger sizes than using trifluoroethanol (TFE). Scanning electron microscopy showed the spherical morphology of the particles, with sizes of 3946 ± 407 nm and 1774 ± 167 nm, respectively for PLGA-Met(DCM) and PLGA-Met(TFE). The FTIR spectroscopy proved the incorporation of metronidazole in the polymer, but without any specific drug-polymer interaction. The release of the drug from the particles was studied in phosphate buffered saline, where a sustained drug release was obtained for at least 41 days. Cytotoxicity evaluation of the drug extract using mesenchymal stem cells (MSCs) showed not hindering the proliferation of MSCs, and the cell phenotype was retained after incubation in the drug containing media. Electrospraying is suggested as a cost-effective and single step process for the preparation of polymeric microparticles for prolonged and controlled release of drug.
Fil: Prabhakaran, Molamma P.. National University Of Singapore; Singapur
Fil: Zamani, Maedeh. National University Of Singapore; Singapur
Fil: Felice, Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Ramakrishna, Seeram. National University Of Singapore; Singapur
Materia
Drug Delivery
Electrospraying
Particle Size
Release
Stem Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50480
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50480
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profilePrabhakaran, Molamma P.Zamani, MaedehFelice, BetianaRamakrishna, SeeramDrug DeliveryElectrosprayingParticle SizeReleaseStem Cellshttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Advanced engineering of materials for the development of drug delivery devices provides scope for novel and versatile strategies for treatment of various diseases. 'Electrospraying' was used to prepare PLGA microparticles and further encapsulate the drug, metronidazole (Met) within the particles to function as a drug delivery system. Two different solvents were utilized for the preparation of drug loaded PLGA particles, whereby the polymeric solution in dichloromethane (DCM) produced particles of bigger sizes than using trifluoroethanol (TFE). Scanning electron microscopy showed the spherical morphology of the particles, with sizes of 3946 ± 407 nm and 1774 ± 167 nm, respectively for PLGA-Met(DCM) and PLGA-Met(TFE). The FTIR spectroscopy proved the incorporation of metronidazole in the polymer, but without any specific drug-polymer interaction. The release of the drug from the particles was studied in phosphate buffered saline, where a sustained drug release was obtained for at least 41 days. Cytotoxicity evaluation of the drug extract using mesenchymal stem cells (MSCs) showed not hindering the proliferation of MSCs, and the cell phenotype was retained after incubation in the drug containing media. Electrospraying is suggested as a cost-effective and single step process for the preparation of polymeric microparticles for prolonged and controlled release of drug.Fil: Prabhakaran, Molamma P.. National University Of Singapore; SingapurFil: Zamani, Maedeh. National University Of Singapore; SingapurFil: Felice, Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Ramakrishna, Seeram. National University Of Singapore; SingapurElsevier Science2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50480Prabhakaran, Molamma P.; Zamani, Maedeh; Felice, Betiana; Ramakrishna, Seeram; Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile; Elsevier Science; Materials Science and Engineering: C; 56; 11; 11-2015; 66-730928-4931CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.msec.2015.06.018info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0928493115301478?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:00Zoai:ri.conicet.gov.ar:11336/50480instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:01.191CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile
title Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile
spellingShingle Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile
Prabhakaran, Molamma P.
Drug Delivery
Electrospraying
Particle Size
Release
Stem Cells
title_short Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile
title_full Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile
title_fullStr Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile
title_full_unstemmed Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile
title_sort Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile
dc.creator.none.fl_str_mv Prabhakaran, Molamma P.
Zamani, Maedeh
Felice, Betiana
Ramakrishna, Seeram
author Prabhakaran, Molamma P.
author_facet Prabhakaran, Molamma P.
Zamani, Maedeh
Felice, Betiana
Ramakrishna, Seeram
author_role author
author2 Zamani, Maedeh
Felice, Betiana
Ramakrishna, Seeram
author2_role author
author
author
dc.subject.none.fl_str_mv Drug Delivery
Electrospraying
Particle Size
Release
Stem Cells
topic Drug Delivery
Electrospraying
Particle Size
Release
Stem Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Advanced engineering of materials for the development of drug delivery devices provides scope for novel and versatile strategies for treatment of various diseases. 'Electrospraying' was used to prepare PLGA microparticles and further encapsulate the drug, metronidazole (Met) within the particles to function as a drug delivery system. Two different solvents were utilized for the preparation of drug loaded PLGA particles, whereby the polymeric solution in dichloromethane (DCM) produced particles of bigger sizes than using trifluoroethanol (TFE). Scanning electron microscopy showed the spherical morphology of the particles, with sizes of 3946 ± 407 nm and 1774 ± 167 nm, respectively for PLGA-Met(DCM) and PLGA-Met(TFE). The FTIR spectroscopy proved the incorporation of metronidazole in the polymer, but without any specific drug-polymer interaction. The release of the drug from the particles was studied in phosphate buffered saline, where a sustained drug release was obtained for at least 41 days. Cytotoxicity evaluation of the drug extract using mesenchymal stem cells (MSCs) showed not hindering the proliferation of MSCs, and the cell phenotype was retained after incubation in the drug containing media. Electrospraying is suggested as a cost-effective and single step process for the preparation of polymeric microparticles for prolonged and controlled release of drug.
Fil: Prabhakaran, Molamma P.. National University Of Singapore; Singapur
Fil: Zamani, Maedeh. National University Of Singapore; Singapur
Fil: Felice, Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Ramakrishna, Seeram. National University Of Singapore; Singapur
description Advanced engineering of materials for the development of drug delivery devices provides scope for novel and versatile strategies for treatment of various diseases. 'Electrospraying' was used to prepare PLGA microparticles and further encapsulate the drug, metronidazole (Met) within the particles to function as a drug delivery system. Two different solvents were utilized for the preparation of drug loaded PLGA particles, whereby the polymeric solution in dichloromethane (DCM) produced particles of bigger sizes than using trifluoroethanol (TFE). Scanning electron microscopy showed the spherical morphology of the particles, with sizes of 3946 ± 407 nm and 1774 ± 167 nm, respectively for PLGA-Met(DCM) and PLGA-Met(TFE). The FTIR spectroscopy proved the incorporation of metronidazole in the polymer, but without any specific drug-polymer interaction. The release of the drug from the particles was studied in phosphate buffered saline, where a sustained drug release was obtained for at least 41 days. Cytotoxicity evaluation of the drug extract using mesenchymal stem cells (MSCs) showed not hindering the proliferation of MSCs, and the cell phenotype was retained after incubation in the drug containing media. Electrospraying is suggested as a cost-effective and single step process for the preparation of polymeric microparticles for prolonged and controlled release of drug.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50480
Prabhakaran, Molamma P.; Zamani, Maedeh; Felice, Betiana; Ramakrishna, Seeram; Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile; Elsevier Science; Materials Science and Engineering: C; 56; 11; 11-2015; 66-73
0928-4931
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50480
identifier_str_mv Prabhakaran, Molamma P.; Zamani, Maedeh; Felice, Betiana; Ramakrishna, Seeram; Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile; Elsevier Science; Materials Science and Engineering: C; 56; 11; 11-2015; 66-73
0928-4931
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.msec.2015.06.018
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0928493115301478?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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