PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior

Autores
Lassalle, Verónica Leticia; Ferreira, María Luján
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: Drug delivery systems (DDS) were designed using insulin as model drug and poly (lactic-co-glycolic) copolymers (PLGA) as polymeric matrix. The carriers were synthesized by direct self-assembly of the insulin and the polyester under mild conditions. RESULTS: The kind and level of association between the protein and the polymer were studied using computational methods (combined MM2/PM3) and spectroscopic tools (Fourier transform infrared (FTIR), energy dispersive X-ray (EDX) and X-ray fluorescence spectroscopy (XFS)). The effect of the number average molecular weight (Mn) of the copolymer on the association efficiency (AE) drug-polymer as well as on the release profile has been explored. Mathematical models were used to predict the insulin release kinetic and mechanism. CONCLUSIONS: Satisfactory protein/PLGA association efficiencies (between 77 and 99%) were registered depending on the Mn of the PLGA. Hydrophobic and hydrophilic interactions were detected between the protein and the polymeric network by computational analysis. In vitro release studies demonstrated that copolyesters of about 8600 and 1500 Da were suitable for the gradual release of insulin while PLGA oligomers of average molecular weight between 700 and 800 Da were unsuitable as DDS. The insulin release kinetics fits well with the Korsmeyer model, following the anomalous transport mechanism. © 2010 Society of Chemical Industry.
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Materia
Biomaterials
Insulin
Plga
Protein Delivery
Release Mechanism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64071
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/64071
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behaviorLassalle, Verónica LeticiaFerreira, María LujánBiomaterialsInsulinPlgaProtein DeliveryRelease Mechanismhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2BACKGROUND: Drug delivery systems (DDS) were designed using insulin as model drug and poly (lactic-co-glycolic) copolymers (PLGA) as polymeric matrix. The carriers were synthesized by direct self-assembly of the insulin and the polyester under mild conditions. RESULTS: The kind and level of association between the protein and the polymer were studied using computational methods (combined MM2/PM3) and spectroscopic tools (Fourier transform infrared (FTIR), energy dispersive X-ray (EDX) and X-ray fluorescence spectroscopy (XFS)). The effect of the number average molecular weight (Mn) of the copolymer on the association efficiency (AE) drug-polymer as well as on the release profile has been explored. Mathematical models were used to predict the insulin release kinetic and mechanism. CONCLUSIONS: Satisfactory protein/PLGA association efficiencies (between 77 and 99%) were registered depending on the Mn of the PLGA. Hydrophobic and hydrophilic interactions were detected between the protein and the polymeric network by computational analysis. In vitro release studies demonstrated that copolyesters of about 8600 and 1500 Da were suitable for the gradual release of insulin while PLGA oligomers of average molecular weight between 700 and 800 Da were unsuitable as DDS. The insulin release kinetics fits well with the Korsmeyer model, following the anomalous transport mechanism. © 2010 Society of Chemical Industry.Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaJohn Wiley & Sons Ltd2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64071Lassalle, Verónica Leticia; Ferreira, María Luján; PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior; John Wiley & Sons Ltd; Journal of Chemical Technology and Biotechnology; 85; 12; 12-2010; 1588-15960268-2575CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jctb.2470info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/jctb.2470info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:24Zoai:ri.conicet.gov.ar:11336/64071instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:24.804CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior
title PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior
spellingShingle PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior
Lassalle, Verónica Leticia
Biomaterials
Insulin
Plga
Protein Delivery
Release Mechanism
title_short PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior
title_full PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior
title_fullStr PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior
title_full_unstemmed PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior
title_sort PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior
dc.creator.none.fl_str_mv Lassalle, Verónica Leticia
Ferreira, María Luján
author Lassalle, Verónica Leticia
author_facet Lassalle, Verónica Leticia
Ferreira, María Luján
author_role author
author2 Ferreira, María Luján
author2_role author
dc.subject.none.fl_str_mv Biomaterials
Insulin
Plga
Protein Delivery
Release Mechanism
topic Biomaterials
Insulin
Plga
Protein Delivery
Release Mechanism
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv BACKGROUND: Drug delivery systems (DDS) were designed using insulin as model drug and poly (lactic-co-glycolic) copolymers (PLGA) as polymeric matrix. The carriers were synthesized by direct self-assembly of the insulin and the polyester under mild conditions. RESULTS: The kind and level of association between the protein and the polymer were studied using computational methods (combined MM2/PM3) and spectroscopic tools (Fourier transform infrared (FTIR), energy dispersive X-ray (EDX) and X-ray fluorescence spectroscopy (XFS)). The effect of the number average molecular weight (Mn) of the copolymer on the association efficiency (AE) drug-polymer as well as on the release profile has been explored. Mathematical models were used to predict the insulin release kinetic and mechanism. CONCLUSIONS: Satisfactory protein/PLGA association efficiencies (between 77 and 99%) were registered depending on the Mn of the PLGA. Hydrophobic and hydrophilic interactions were detected between the protein and the polymeric network by computational analysis. In vitro release studies demonstrated that copolyesters of about 8600 and 1500 Da were suitable for the gradual release of insulin while PLGA oligomers of average molecular weight between 700 and 800 Da were unsuitable as DDS. The insulin release kinetics fits well with the Korsmeyer model, following the anomalous transport mechanism. © 2010 Society of Chemical Industry.
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
description BACKGROUND: Drug delivery systems (DDS) were designed using insulin as model drug and poly (lactic-co-glycolic) copolymers (PLGA) as polymeric matrix. The carriers were synthesized by direct self-assembly of the insulin and the polyester under mild conditions. RESULTS: The kind and level of association between the protein and the polymer were studied using computational methods (combined MM2/PM3) and spectroscopic tools (Fourier transform infrared (FTIR), energy dispersive X-ray (EDX) and X-ray fluorescence spectroscopy (XFS)). The effect of the number average molecular weight (Mn) of the copolymer on the association efficiency (AE) drug-polymer as well as on the release profile has been explored. Mathematical models were used to predict the insulin release kinetic and mechanism. CONCLUSIONS: Satisfactory protein/PLGA association efficiencies (between 77 and 99%) were registered depending on the Mn of the PLGA. Hydrophobic and hydrophilic interactions were detected between the protein and the polymeric network by computational analysis. In vitro release studies demonstrated that copolyesters of about 8600 and 1500 Da were suitable for the gradual release of insulin while PLGA oligomers of average molecular weight between 700 and 800 Da were unsuitable as DDS. The insulin release kinetics fits well with the Korsmeyer model, following the anomalous transport mechanism. © 2010 Society of Chemical Industry.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64071
Lassalle, Verónica Leticia; Ferreira, María Luján; PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior; John Wiley & Sons Ltd; Journal of Chemical Technology and Biotechnology; 85; 12; 12-2010; 1588-1596
0268-2575
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64071
identifier_str_mv Lassalle, Verónica Leticia; Ferreira, María Luján; PLGA based drug delivery systems (DDS) for the sustained release of insulin: Insight into the protein/polyester interactions and the insulin release behavior; John Wiley & Sons Ltd; Journal of Chemical Technology and Biotechnology; 85; 12; 12-2010; 1588-1596
0268-2575
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jctb.2470
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/jctb.2470
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269285174476800
score 13.13397

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