Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species

Autores
Bozzi, Jorge A.; Liepelt, Sascha; Ohneiser, Sebastián; Gallo, Leonardo; Marchelli, Paula; Leyer, Ilona; Ziegenhagen, Birgit; Mengel, Christina
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.
Fil: Bozzi, Jorge A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte; Argentina
Fil: Liepelt, Sascha. University of Marburg; Alemania
Fil: Ohneiser, Sebastián. University of Marburg; Alemania
Fil: Gallo, Leonardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte; Argentina
Fil: Marchelli, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte; Argentina
Fil: Leyer, Ilona. University of Marburg; Alemania. University of Geisenheim; Alemania
Fil: Ziegenhagen, Birgit. University of Marburg; Alemania
Fil: Mengel, Christina. University of Marburg; Alemania
Materia
Next Generation Sequencing
Nuclear Ssr Marker
River Margins
Salix Humboldtiana
Salicaceae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43927
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related SpeciesBozzi, Jorge A.Liepelt, SaschaOhneiser, SebastiánGallo, LeonardoMarchelli, PaulaLeyer, IlonaZiegenhagen, BirgitMengel, ChristinaNext Generation SequencingNuclear Ssr MarkerRiver MarginsSalix HumboldtianaSalicaceaehttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.Fil: Bozzi, Jorge A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte; ArgentinaFil: Liepelt, Sascha. University of Marburg; AlemaniaFil: Ohneiser, Sebastián. University of Marburg; AlemaniaFil: Gallo, Leonardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte; ArgentinaFil: Marchelli, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte; ArgentinaFil: Leyer, Ilona. University of Marburg; Alemania. University of Geisenheim; AlemaniaFil: Ziegenhagen, Birgit. University of Marburg; AlemaniaFil: Mengel, Christina. University of Marburg; AlemaniaBotanical Society of America2015-04-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43927Bozzi, Jorge A.; Liepelt, Sascha; Ohneiser, Sebastián; Gallo, Leonardo; Marchelli, Paula; et al.; Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species; Botanical Society of America; Applications in Plant Sciences; 3; 4; 7-4-2015; 14-001201550-74162168-0450CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.3732/apps.1400120info:eu-repo/semantics/altIdentifier/doi/10.3732/apps.1400120info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:52:14Zoai:ri.conicet.gov.ar:11336/43927instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:52:14.607CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species
title Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species
spellingShingle Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species
Bozzi, Jorge A.
Next Generation Sequencing
Nuclear Ssr Marker
River Margins
Salix Humboldtiana
Salicaceae
title_short Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species
title_full Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species
title_fullStr Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species
title_full_unstemmed Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species
title_sort Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species
dc.creator.none.fl_str_mv Bozzi, Jorge A.
Liepelt, Sascha
Ohneiser, Sebastián
Gallo, Leonardo
Marchelli, Paula
Leyer, Ilona
Ziegenhagen, Birgit
Mengel, Christina
author Bozzi, Jorge A.
author_facet Bozzi, Jorge A.
Liepelt, Sascha
Ohneiser, Sebastián
Gallo, Leonardo
Marchelli, Paula
Leyer, Ilona
Ziegenhagen, Birgit
Mengel, Christina
author_role author
author2 Liepelt, Sascha
Ohneiser, Sebastián
Gallo, Leonardo
Marchelli, Paula
Leyer, Ilona
Ziegenhagen, Birgit
Mengel, Christina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Next Generation Sequencing
Nuclear Ssr Marker
River Margins
Salix Humboldtiana
Salicaceae
topic Next Generation Sequencing
Nuclear Ssr Marker
River Margins
Salix Humboldtiana
Salicaceae
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.
Fil: Bozzi, Jorge A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte; Argentina
Fil: Liepelt, Sascha. University of Marburg; Alemania
Fil: Ohneiser, Sebastián. University of Marburg; Alemania
Fil: Gallo, Leonardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte; Argentina
Fil: Marchelli, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte; Argentina
Fil: Leyer, Ilona. University of Marburg; Alemania. University of Geisenheim; Alemania
Fil: Ziegenhagen, Birgit. University of Marburg; Alemania
Fil: Mengel, Christina. University of Marburg; Alemania
description Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43927
Bozzi, Jorge A.; Liepelt, Sascha; Ohneiser, Sebastián; Gallo, Leonardo; Marchelli, Paula; et al.; Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species; Botanical Society of America; Applications in Plant Sciences; 3; 4; 7-4-2015; 14-00120
1550-7416
2168-0450
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43927
identifier_str_mv Bozzi, Jorge A.; Liepelt, Sascha; Ohneiser, Sebastián; Gallo, Leonardo; Marchelli, Paula; et al.; Characterization of 23 Polymorphic SSR Markers in Salix humboldtiana (Salicaceae) Using Next-Generation Sequencing and Cross-Amplification from Related Species; Botanical Society of America; Applications in Plant Sciences; 3; 4; 7-4-2015; 14-00120
1550-7416
2168-0450
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.3732/apps.1400120
info:eu-repo/semantics/altIdentifier/doi/10.3732/apps.1400120
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Botanical Society of America
publisher.none.fl_str_mv Botanical Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083049937174528
score 13.22299

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