Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species
- Autores
- Bozzi, Jorge Alfredo; Liepelt, Sascha; Ohneiser, Sebastian; Gallo, Leonardo Ariel; Marchelli, Paula; Leyer, Ilona; Ziegenhagen, Birgit; Mengel, Christina
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.
EEA Bariloche
Fil: Bozzi, Jorge Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Liepelt, Sascha. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania
Fil: Ohneiser, Sebastian. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania
Fil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leyer, Ilona. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania. University of Geisenheim. Institute of Botany, Plant Ecology and Nature Conservation; Alemania
Fil: Ziegenhagen, Birgit. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania
Fil: Mengel, Christina. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania - Fuente
- Applications in Plant Sciences 3 (4) : 1400120 ( April 2015)
- Materia
-
Salix
Árboles Maderables
Marcadores Genéticos
Secuencia Nucleotídica
Timber Trees
Genetic Markers
Nucleotide Sequence
Salix humboldtiana
Marcadores Moleculares - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4555
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Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related speciesBozzi, Jorge AlfredoLiepelt, SaschaOhneiser, SebastianGallo, Leonardo ArielMarchelli, PaulaLeyer, IlonaZiegenhagen, BirgitMengel, ChristinaSalixÁrboles MaderablesMarcadores GenéticosSecuencia NucleotídicaTimber TreesGenetic MarkersNucleotide SequenceSalix humboldtianaMarcadores MolecularesPremise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.EEA BarilocheFil: Bozzi, Jorge Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: Liepelt, Sascha. University of Marburg. Faculty of Biology. Conservation Biology Group; AlemaniaFil: Ohneiser, Sebastian. University of Marburg. Faculty of Biology. Conservation Biology Group; AlemaniaFil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leyer, Ilona. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania. University of Geisenheim. Institute of Botany, Plant Ecology and Nature Conservation; AlemaniaFil: Ziegenhagen, Birgit. University of Marburg. Faculty of Biology. Conservation Biology Group; AlemaniaFil: Mengel, Christina. University of Marburg. Faculty of Biology. Conservation Biology Group; AlemaniaBotanical Society of America2019-03-08T12:47:02Z2019-03-08T12:47:02Z2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/apps.1400120http://hdl.handle.net/20.500.12123/45551550-7416https://doi.org/10.3732/apps.1400120Applications in Plant Sciences 3 (4) : 1400120 ( April 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:50Zoai:localhost:20.500.12123/4555instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:51.57INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
title |
Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
spellingShingle |
Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species Bozzi, Jorge Alfredo Salix Árboles Maderables Marcadores Genéticos Secuencia Nucleotídica Timber Trees Genetic Markers Nucleotide Sequence Salix humboldtiana Marcadores Moleculares |
title_short |
Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
title_full |
Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
title_fullStr |
Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
title_full_unstemmed |
Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
title_sort |
Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species |
dc.creator.none.fl_str_mv |
Bozzi, Jorge Alfredo Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo Ariel Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina |
author |
Bozzi, Jorge Alfredo |
author_facet |
Bozzi, Jorge Alfredo Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo Ariel Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina |
author_role |
author |
author2 |
Liepelt, Sascha Ohneiser, Sebastian Gallo, Leonardo Ariel Marchelli, Paula Leyer, Ilona Ziegenhagen, Birgit Mengel, Christina |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Salix Árboles Maderables Marcadores Genéticos Secuencia Nucleotídica Timber Trees Genetic Markers Nucleotide Sequence Salix humboldtiana Marcadores Moleculares |
topic |
Salix Árboles Maderables Marcadores Genéticos Secuencia Nucleotídica Timber Trees Genetic Markers Nucleotide Sequence Salix humboldtiana Marcadores Moleculares |
dc.description.none.fl_txt_mv |
Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina. EEA Bariloche Fil: Bozzi, Jorge Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina Fil: Liepelt, Sascha. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania Fil: Ohneiser, Sebastian. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania Fil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Leyer, Ilona. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania. University of Geisenheim. Institute of Botany, Plant Ecology and Nature Conservation; Alemania Fil: Ziegenhagen, Birgit. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania Fil: Mengel, Christina. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania |
description |
Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04 2019-03-08T12:47:02Z 2019-03-08T12:47:02Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/apps.1400120 http://hdl.handle.net/20.500.12123/4555 1550-7416 https://doi.org/10.3732/apps.1400120 |
url |
https://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/apps.1400120 http://hdl.handle.net/20.500.12123/4555 https://doi.org/10.3732/apps.1400120 |
identifier_str_mv |
1550-7416 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Botanical Society of America |
publisher.none.fl_str_mv |
Botanical Society of America |
dc.source.none.fl_str_mv |
Applications in Plant Sciences 3 (4) : 1400120 ( April 2015) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1842341363908083712 |
score |
12.623145 |