Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species

Autores
Bozzi, Jorge Alfredo; Liepelt, Sascha; Ohneiser, Sebastian; Gallo, Leonardo Ariel; Marchelli, Paula; Leyer, Ilona; Ziegenhagen, Birgit; Mengel, Christina
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.
EEA Bariloche
Fil: Bozzi, Jorge Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Liepelt, Sascha. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania
Fil: Ohneiser, Sebastian. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania
Fil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leyer, Ilona. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania. University of Geisenheim. Institute of Botany, Plant Ecology and Nature Conservation; Alemania
Fil: Ziegenhagen, Birgit. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania
Fil: Mengel, Christina. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania
Fuente
Applications in Plant Sciences 3 (4) : 1400120 ( April 2015)
Materia
Salix
Árboles Maderables
Marcadores Genéticos
Secuencia Nucleotídica
Timber Trees
Genetic Markers
Nucleotide Sequence
Salix humboldtiana
Marcadores Moleculares
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4555
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network_name_str INTA Digital (INTA)
spelling Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related speciesBozzi, Jorge AlfredoLiepelt, SaschaOhneiser, SebastianGallo, Leonardo ArielMarchelli, PaulaLeyer, IlonaZiegenhagen, BirgitMengel, ChristinaSalixÁrboles MaderablesMarcadores GenéticosSecuencia NucleotídicaTimber TreesGenetic MarkersNucleotide SequenceSalix humboldtianaMarcadores MolecularesPremise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.EEA BarilocheFil: Bozzi, Jorge Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: Liepelt, Sascha. University of Marburg. Faculty of Biology. Conservation Biology Group; AlemaniaFil: Ohneiser, Sebastian. University of Marburg. Faculty of Biology. Conservation Biology Group; AlemaniaFil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leyer, Ilona. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania. University of Geisenheim. Institute of Botany, Plant Ecology and Nature Conservation; AlemaniaFil: Ziegenhagen, Birgit. University of Marburg. Faculty of Biology. Conservation Biology Group; AlemaniaFil: Mengel, Christina. University of Marburg. Faculty of Biology. Conservation Biology Group; AlemaniaBotanical Society of America2019-03-08T12:47:02Z2019-03-08T12:47:02Z2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/apps.1400120http://hdl.handle.net/20.500.12123/45551550-7416https://doi.org/10.3732/apps.1400120Applications in Plant Sciences 3 (4) : 1400120 ( April 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:35Zoai:localhost:20.500.12123/4555instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:35.827INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species
title Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species
spellingShingle Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species
Bozzi, Jorge Alfredo
Salix
Árboles Maderables
Marcadores Genéticos
Secuencia Nucleotídica
Timber Trees
Genetic Markers
Nucleotide Sequence
Salix humboldtiana
Marcadores Moleculares
title_short Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species
title_full Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species
title_fullStr Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species
title_full_unstemmed Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species
title_sort Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next‐generation sequencing and cross‐amplification from related species
dc.creator.none.fl_str_mv Bozzi, Jorge Alfredo
Liepelt, Sascha
Ohneiser, Sebastian
Gallo, Leonardo Ariel
Marchelli, Paula
Leyer, Ilona
Ziegenhagen, Birgit
Mengel, Christina
author Bozzi, Jorge Alfredo
author_facet Bozzi, Jorge Alfredo
Liepelt, Sascha
Ohneiser, Sebastian
Gallo, Leonardo Ariel
Marchelli, Paula
Leyer, Ilona
Ziegenhagen, Birgit
Mengel, Christina
author_role author
author2 Liepelt, Sascha
Ohneiser, Sebastian
Gallo, Leonardo Ariel
Marchelli, Paula
Leyer, Ilona
Ziegenhagen, Birgit
Mengel, Christina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Salix
Árboles Maderables
Marcadores Genéticos
Secuencia Nucleotídica
Timber Trees
Genetic Markers
Nucleotide Sequence
Salix humboldtiana
Marcadores Moleculares
topic Salix
Árboles Maderables
Marcadores Genéticos
Secuencia Nucleotídica
Timber Trees
Genetic Markers
Nucleotide Sequence
Salix humboldtiana
Marcadores Moleculares
dc.description.none.fl_txt_mv Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.
EEA Bariloche
Fil: Bozzi, Jorge Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Liepelt, Sascha. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania
Fil: Ohneiser, Sebastian. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania
Fil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leyer, Ilona. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania. University of Geisenheim. Institute of Botany, Plant Ecology and Nature Conservation; Alemania
Fil: Ziegenhagen, Birgit. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania
Fil: Mengel, Christina. University of Marburg. Faculty of Biology. Conservation Biology Group; Alemania
description Premise of the study: We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next‐generation sequencing. Methods and Results: To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus. Conclusions: The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
2019-03-08T12:47:02Z
2019-03-08T12:47:02Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/apps.1400120
http://hdl.handle.net/20.500.12123/4555
1550-7416
https://doi.org/10.3732/apps.1400120
url https://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/apps.1400120
http://hdl.handle.net/20.500.12123/4555
https://doi.org/10.3732/apps.1400120
identifier_str_mv 1550-7416
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Botanical Society of America
publisher.none.fl_str_mv Botanical Society of America
dc.source.none.fl_str_mv Applications in Plant Sciences 3 (4) : 1400120 ( April 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619131385544704
score 12.558318

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