The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021

Autores
Costa, Daniela; Amarelle, Vanesa; Valverde, Claudio Fabián; O`Brian, Mark R.; Fabiano, Elena
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work we found that the bfr gene of the rhizobial species Ensifer meliloti, encoding a bacterioferritin iron storage protein, is involved in iron homeostasis and the oxidative stress response. This gene is located downstream of and overlapping the smc03787 open reading frame (ORF). No well-predicted RirA or Irr boxes were found in the region immediately upstream of the bfr gene although two presumptive RirA boxes and one presumptive Irr box were present in the putative promoter of smc03787. We demonstrate that bfr gene expression is enhanced under iron-sufficient conditions and that Irr and RirA modulate this expression. The pattern of bfr gene expression as well as the response to Irr and RirA is inversely correlated to that of smc03787. Moreover, our results suggest that the small RNA SmelC759 participates in RirA- and Irr-mediated regulation of bfr expression and that additional unknown factors are involved in iron-dependent regulation.
Fil: Costa, Daniela. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Amarelle, Vanesa. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Valverde, Claudio Fabián. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: O`Brian, Mark R.. State University of New York; Estados Unidos
Fil: Fabiano, Elena. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Materia
IRON METABOLISM
BACTERIOFERRITIN
SINORHIZOBIUM MELILOTI
GENETIC REGULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40963
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40963
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021Costa, DanielaAmarelle, VanesaValverde, Claudio FabiánO`Brian, Mark R.Fabiano, ElenaIRON METABOLISMBACTERIOFERRITINSINORHIZOBIUM MELILOTIGENETIC REGULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this work we found that the bfr gene of the rhizobial species Ensifer meliloti, encoding a bacterioferritin iron storage protein, is involved in iron homeostasis and the oxidative stress response. This gene is located downstream of and overlapping the smc03787 open reading frame (ORF). No well-predicted RirA or Irr boxes were found in the region immediately upstream of the bfr gene although two presumptive RirA boxes and one presumptive Irr box were present in the putative promoter of smc03787. We demonstrate that bfr gene expression is enhanced under iron-sufficient conditions and that Irr and RirA modulate this expression. The pattern of bfr gene expression as well as the response to Irr and RirA is inversely correlated to that of smc03787. Moreover, our results suggest that the small RNA SmelC759 participates in RirA- and Irr-mediated regulation of bfr expression and that additional unknown factors are involved in iron-dependent regulation.Fil: Costa, Daniela. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Amarelle, Vanesa. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Valverde, Claudio Fabián. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: O`Brian, Mark R.. State University of New York; Estados UnidosFil: Fabiano, Elena. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayAmerican Society for Microbiology2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40963Costa, Daniela; Amarelle, Vanesa; Valverde, Claudio Fabián; O`Brian, Mark R.; Fabiano, Elena; The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021; American Society for Microbiology; Applied And Environmental Microbiology; 83; 16; 6-20170099-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.00895-17info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/83/16/e00895-17info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:51Zoai:ri.conicet.gov.ar:11336/40963instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:52.197CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021
title The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021
spellingShingle The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021
Costa, Daniela
IRON METABOLISM
BACTERIOFERRITIN
SINORHIZOBIUM MELILOTI
GENETIC REGULATION
title_short The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021
title_full The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021
title_fullStr The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021
title_full_unstemmed The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021
title_sort The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021
dc.creator.none.fl_str_mv Costa, Daniela
Amarelle, Vanesa
Valverde, Claudio Fabián
O`Brian, Mark R.
Fabiano, Elena
author Costa, Daniela
author_facet Costa, Daniela
Amarelle, Vanesa
Valverde, Claudio Fabián
O`Brian, Mark R.
Fabiano, Elena
author_role author
author2 Amarelle, Vanesa
Valverde, Claudio Fabián
O`Brian, Mark R.
Fabiano, Elena
author2_role author
author
author
author
dc.subject.none.fl_str_mv IRON METABOLISM
BACTERIOFERRITIN
SINORHIZOBIUM MELILOTI
GENETIC REGULATION
topic IRON METABOLISM
BACTERIOFERRITIN
SINORHIZOBIUM MELILOTI
GENETIC REGULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work we found that the bfr gene of the rhizobial species Ensifer meliloti, encoding a bacterioferritin iron storage protein, is involved in iron homeostasis and the oxidative stress response. This gene is located downstream of and overlapping the smc03787 open reading frame (ORF). No well-predicted RirA or Irr boxes were found in the region immediately upstream of the bfr gene although two presumptive RirA boxes and one presumptive Irr box were present in the putative promoter of smc03787. We demonstrate that bfr gene expression is enhanced under iron-sufficient conditions and that Irr and RirA modulate this expression. The pattern of bfr gene expression as well as the response to Irr and RirA is inversely correlated to that of smc03787. Moreover, our results suggest that the small RNA SmelC759 participates in RirA- and Irr-mediated regulation of bfr expression and that additional unknown factors are involved in iron-dependent regulation.
Fil: Costa, Daniela. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Amarelle, Vanesa. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Valverde, Claudio Fabián. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: O`Brian, Mark R.. State University of New York; Estados Unidos
Fil: Fabiano, Elena. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
description In this work we found that the bfr gene of the rhizobial species Ensifer meliloti, encoding a bacterioferritin iron storage protein, is involved in iron homeostasis and the oxidative stress response. This gene is located downstream of and overlapping the smc03787 open reading frame (ORF). No well-predicted RirA or Irr boxes were found in the region immediately upstream of the bfr gene although two presumptive RirA boxes and one presumptive Irr box were present in the putative promoter of smc03787. We demonstrate that bfr gene expression is enhanced under iron-sufficient conditions and that Irr and RirA modulate this expression. The pattern of bfr gene expression as well as the response to Irr and RirA is inversely correlated to that of smc03787. Moreover, our results suggest that the small RNA SmelC759 participates in RirA- and Irr-mediated regulation of bfr expression and that additional unknown factors are involved in iron-dependent regulation.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40963
Costa, Daniela; Amarelle, Vanesa; Valverde, Claudio Fabián; O`Brian, Mark R.; Fabiano, Elena; The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021; American Society for Microbiology; Applied And Environmental Microbiology; 83; 16; 6-2017
0099-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40963
identifier_str_mv Costa, Daniela; Amarelle, Vanesa; Valverde, Claudio Fabián; O`Brian, Mark R.; Fabiano, Elena; The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021; American Society for Microbiology; Applied And Environmental Microbiology; 83; 16; 6-2017
0099-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.00895-17
info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/83/16/e00895-17
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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