Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa

Autores
Bianchini, Michele; Levy, Estrella Mariel; Zucchini, Cinzia; Pinski, Victor; Macagno, Carlo; De Sanctis, Paola; Valvassori, Luisa; Carinci, Paolo; Mordoh, Jose
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The causative molecular pathways underlying the pathogenesis of colorectal cancer (CRC) need to be better characterized. The purpose of our study was to better understand the genetic mechanism of oncogenesis for human colorectal cancer and to identify new potential tumor markers of use in clinical practice. We used cDNA microarrays to compare gene expression profiles of colorectal biopsies from 25 CRC patients and 13 normal mucosa from adjacent non-cancerous tissues. Findings were validated by real-time PCR; in addition, western blotting and immunochemistry analysis were carried out as further confirmation of differential expression at a protein level. Comparing cancerous tissues with normal colonic mucosa we identified 584 known genes differentially expressed to a significant degree (p<0.001). Many of the transcripts that were more abundant in tumors than in non-neoplastic tissues appear to reflect important events for colon carcinogenesis. For example, a significant number of these genes serve as apoptotic inhibitors (e.g. BFAR, BIRC1, BIRC6). Furthermore, we observed the simultaneous up-regulation of HLA-E and the down-regulation of beta2-microglobulin; these genes strongly support a potential tumor escape strategy from immune surveillance in colon cancer tissues. Our study provides new gene candidates in the pathogenesis of human CRC disease. From our results we hypothesize that CRC cells escape immune surveillance through a specific gene expression alteration; moreover, over-expression of several survival genes seems to confer a more anti-apoptotic phenotype. These genes are involved in pathways not previously implicated in CRC pathogenesis and they may provide new targets for therapy.
Fil: Bianchini, Michele. Fundación P/la Invest.y Prevención del Cancer. Centro de Investigaciones Oncologicas; Argentina
Fil: Levy, Estrella Mariel. Fundación P/la Invest.y Prevención del Cancer. Centro de Investigaciones Oncologicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Zucchini, Cinzia. Università di Bologna; Italia
Fil: Pinski, Victor. Universidad de Buenos Aires; Argentina
Fil: Macagno, Carlo. Universidad de Buenos Aires; Argentina
Fil: De Sanctis, Paola. Università di Bologna; Italia
Fil: Valvassori, Luisa. Università di Bologna; Italia
Fil: Carinci, Paolo. Università di Bologna; Italia
Fil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Fundación P/la Invest.y Prevención del Cancer. Centro de Investigaciones Oncologicas; Argentina
Materia
COLON CANCER
cDNA MICROARRAY
GENE EXPRESSION PROFILES
ANTI-APOPTOTIC PHENOTYPE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96513
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96513
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosaBianchini, MicheleLevy, Estrella MarielZucchini, CinziaPinski, VictorMacagno, CarloDe Sanctis, PaolaValvassori, LuisaCarinci, PaoloMordoh, JoseCOLON CANCERcDNA MICROARRAYGENE EXPRESSION PROFILESANTI-APOPTOTIC PHENOTYPEhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The causative molecular pathways underlying the pathogenesis of colorectal cancer (CRC) need to be better characterized. The purpose of our study was to better understand the genetic mechanism of oncogenesis for human colorectal cancer and to identify new potential tumor markers of use in clinical practice. We used cDNA microarrays to compare gene expression profiles of colorectal biopsies from 25 CRC patients and 13 normal mucosa from adjacent non-cancerous tissues. Findings were validated by real-time PCR; in addition, western blotting and immunochemistry analysis were carried out as further confirmation of differential expression at a protein level. Comparing cancerous tissues with normal colonic mucosa we identified 584 known genes differentially expressed to a significant degree (p<0.001). Many of the transcripts that were more abundant in tumors than in non-neoplastic tissues appear to reflect important events for colon carcinogenesis. For example, a significant number of these genes serve as apoptotic inhibitors (e.g. BFAR, BIRC1, BIRC6). Furthermore, we observed the simultaneous up-regulation of HLA-E and the down-regulation of beta2-microglobulin; these genes strongly support a potential tumor escape strategy from immune surveillance in colon cancer tissues. Our study provides new gene candidates in the pathogenesis of human CRC disease. From our results we hypothesize that CRC cells escape immune surveillance through a specific gene expression alteration; moreover, over-expression of several survival genes seems to confer a more anti-apoptotic phenotype. These genes are involved in pathways not previously implicated in CRC pathogenesis and they may provide new targets for therapy.Fil: Bianchini, Michele. Fundación P/la Invest.y Prevención del Cancer. Centro de Investigaciones Oncologicas; ArgentinaFil: Levy, Estrella Mariel. Fundación P/la Invest.y Prevención del Cancer. Centro de Investigaciones Oncologicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Zucchini, Cinzia. Università di Bologna; ItaliaFil: Pinski, Victor. Universidad de Buenos Aires; ArgentinaFil: Macagno, Carlo. Universidad de Buenos Aires; ArgentinaFil: De Sanctis, Paola. Università di Bologna; ItaliaFil: Valvassori, Luisa. Università di Bologna; ItaliaFil: Carinci, Paolo. Università di Bologna; ItaliaFil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Fundación P/la Invest.y Prevención del Cancer. Centro de Investigaciones Oncologicas; ArgentinaSpandidos Publications2006-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96513Bianchini, Michele; Levy, Estrella Mariel; Zucchini, Cinzia; Pinski, Victor; Macagno, Carlo; et al.; Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa; Spandidos Publications; International Journal of Oncology; 29; 1; 7-2006; 83-941019-64391791-2423CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3892/ijo.29.1.83info:eu-repo/semantics/altIdentifier/url/https://www.spandidos-publications.com/10.3892/ijo.29.1.83info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:12Zoai:ri.conicet.gov.ar:11336/96513instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:12.66CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa
title Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa
spellingShingle Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa
Bianchini, Michele
COLON CANCER
cDNA MICROARRAY
GENE EXPRESSION PROFILES
ANTI-APOPTOTIC PHENOTYPE
title_short Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa
title_full Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa
title_fullStr Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa
title_full_unstemmed Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa
title_sort Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa
dc.creator.none.fl_str_mv Bianchini, Michele
Levy, Estrella Mariel
Zucchini, Cinzia
Pinski, Victor
Macagno, Carlo
De Sanctis, Paola
Valvassori, Luisa
Carinci, Paolo
Mordoh, Jose
author Bianchini, Michele
author_facet Bianchini, Michele
Levy, Estrella Mariel
Zucchini, Cinzia
Pinski, Victor
Macagno, Carlo
De Sanctis, Paola
Valvassori, Luisa
Carinci, Paolo
Mordoh, Jose
author_role author
author2 Levy, Estrella Mariel
Zucchini, Cinzia
Pinski, Victor
Macagno, Carlo
De Sanctis, Paola
Valvassori, Luisa
Carinci, Paolo
Mordoh, Jose
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COLON CANCER
cDNA MICROARRAY
GENE EXPRESSION PROFILES
ANTI-APOPTOTIC PHENOTYPE
topic COLON CANCER
cDNA MICROARRAY
GENE EXPRESSION PROFILES
ANTI-APOPTOTIC PHENOTYPE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The causative molecular pathways underlying the pathogenesis of colorectal cancer (CRC) need to be better characterized. The purpose of our study was to better understand the genetic mechanism of oncogenesis for human colorectal cancer and to identify new potential tumor markers of use in clinical practice. We used cDNA microarrays to compare gene expression profiles of colorectal biopsies from 25 CRC patients and 13 normal mucosa from adjacent non-cancerous tissues. Findings were validated by real-time PCR; in addition, western blotting and immunochemistry analysis were carried out as further confirmation of differential expression at a protein level. Comparing cancerous tissues with normal colonic mucosa we identified 584 known genes differentially expressed to a significant degree (p<0.001). Many of the transcripts that were more abundant in tumors than in non-neoplastic tissues appear to reflect important events for colon carcinogenesis. For example, a significant number of these genes serve as apoptotic inhibitors (e.g. BFAR, BIRC1, BIRC6). Furthermore, we observed the simultaneous up-regulation of HLA-E and the down-regulation of beta2-microglobulin; these genes strongly support a potential tumor escape strategy from immune surveillance in colon cancer tissues. Our study provides new gene candidates in the pathogenesis of human CRC disease. From our results we hypothesize that CRC cells escape immune surveillance through a specific gene expression alteration; moreover, over-expression of several survival genes seems to confer a more anti-apoptotic phenotype. These genes are involved in pathways not previously implicated in CRC pathogenesis and they may provide new targets for therapy.
Fil: Bianchini, Michele. Fundación P/la Invest.y Prevención del Cancer. Centro de Investigaciones Oncologicas; Argentina
Fil: Levy, Estrella Mariel. Fundación P/la Invest.y Prevención del Cancer. Centro de Investigaciones Oncologicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Zucchini, Cinzia. Università di Bologna; Italia
Fil: Pinski, Victor. Universidad de Buenos Aires; Argentina
Fil: Macagno, Carlo. Universidad de Buenos Aires; Argentina
Fil: De Sanctis, Paola. Università di Bologna; Italia
Fil: Valvassori, Luisa. Università di Bologna; Italia
Fil: Carinci, Paolo. Università di Bologna; Italia
Fil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Fundación P/la Invest.y Prevención del Cancer. Centro de Investigaciones Oncologicas; Argentina
description The causative molecular pathways underlying the pathogenesis of colorectal cancer (CRC) need to be better characterized. The purpose of our study was to better understand the genetic mechanism of oncogenesis for human colorectal cancer and to identify new potential tumor markers of use in clinical practice. We used cDNA microarrays to compare gene expression profiles of colorectal biopsies from 25 CRC patients and 13 normal mucosa from adjacent non-cancerous tissues. Findings were validated by real-time PCR; in addition, western blotting and immunochemistry analysis were carried out as further confirmation of differential expression at a protein level. Comparing cancerous tissues with normal colonic mucosa we identified 584 known genes differentially expressed to a significant degree (p<0.001). Many of the transcripts that were more abundant in tumors than in non-neoplastic tissues appear to reflect important events for colon carcinogenesis. For example, a significant number of these genes serve as apoptotic inhibitors (e.g. BFAR, BIRC1, BIRC6). Furthermore, we observed the simultaneous up-regulation of HLA-E and the down-regulation of beta2-microglobulin; these genes strongly support a potential tumor escape strategy from immune surveillance in colon cancer tissues. Our study provides new gene candidates in the pathogenesis of human CRC disease. From our results we hypothesize that CRC cells escape immune surveillance through a specific gene expression alteration; moreover, over-expression of several survival genes seems to confer a more anti-apoptotic phenotype. These genes are involved in pathways not previously implicated in CRC pathogenesis and they may provide new targets for therapy.
publishDate 2006
dc.date.none.fl_str_mv 2006-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96513
Bianchini, Michele; Levy, Estrella Mariel; Zucchini, Cinzia; Pinski, Victor; Macagno, Carlo; et al.; Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa; Spandidos Publications; International Journal of Oncology; 29; 1; 7-2006; 83-94
1019-6439
1791-2423
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96513
identifier_str_mv Bianchini, Michele; Levy, Estrella Mariel; Zucchini, Cinzia; Pinski, Victor; Macagno, Carlo; et al.; Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa; Spandidos Publications; International Journal of Oncology; 29; 1; 7-2006; 83-94
1019-6439
1791-2423
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3892/ijo.29.1.83
info:eu-repo/semantics/altIdentifier/url/https://www.spandidos-publications.com/10.3892/ijo.29.1.83
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Spandidos Publications
publisher.none.fl_str_mv Spandidos Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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