Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress

Autores
Dos Santos Ferreira, Veronica Beatriz; Rocchetta, Iara; Conforti, Visitacion Teresa D.; Bench, Shellie; Feldman, Robert; Levin, Mariano Jorge
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e- 10). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response.
Fil: Dos Santos Ferreira, Veronica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina
Fil: Rocchetta, Iara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Conforti, Visitacion Teresa D.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Bench, Shellie. University of California; Estados Unidos
Fil: Feldman, Robert. University of California; Estados Unidos. SymBio Corporation; Estados Unidos
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina. lnstitut Cochin; Francia
Materia
Cdna Library
Chromium
Est
Microarray
Streptomycin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79885
Acceder
id CONICETDig_9616abe96a66d2761dc02dbd72324e9e
oai_identifier_str oai:ri.conicet.gov.ar:11336/79885
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stressDos Santos Ferreira, Veronica BeatrizRocchetta, IaraConforti, Visitacion Teresa D.Bench, ShellieFeldman, RobertLevin, Mariano JorgeCdna LibraryChromiumEstMicroarrayStreptomycinhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e- 10). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response.Fil: Dos Santos Ferreira, Veronica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; ArgentinaFil: Rocchetta, Iara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Conforti, Visitacion Teresa D.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Bench, Shellie. University of California; Estados UnidosFil: Feldman, Robert. University of California; Estados Unidos. SymBio Corporation; Estados UnidosFil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina. lnstitut Cochin; FranciaElsevier Science2007-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79885Dos Santos Ferreira, Veronica Beatriz; Rocchetta, Iara; Conforti, Visitacion Teresa D.; Bench, Shellie; Feldman, Robert; et al.; Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress; Elsevier Science; Gene; 389; 2; 3-2007; 136-1450378-1119CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378111906006858info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2006.10.023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:59:21Zoai:ri.conicet.gov.ar:11336/79885instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:59:21.991CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
title Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
spellingShingle Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
Dos Santos Ferreira, Veronica Beatriz
Cdna Library
Chromium
Est
Microarray
Streptomycin
title_short Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
title_full Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
title_fullStr Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
title_full_unstemmed Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
title_sort Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress
dc.creator.none.fl_str_mv Dos Santos Ferreira, Veronica Beatriz
Rocchetta, Iara
Conforti, Visitacion Teresa D.
Bench, Shellie
Feldman, Robert
Levin, Mariano Jorge
author Dos Santos Ferreira, Veronica Beatriz
author_facet Dos Santos Ferreira, Veronica Beatriz
Rocchetta, Iara
Conforti, Visitacion Teresa D.
Bench, Shellie
Feldman, Robert
Levin, Mariano Jorge
author_role author
author2 Rocchetta, Iara
Conforti, Visitacion Teresa D.
Bench, Shellie
Feldman, Robert
Levin, Mariano Jorge
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cdna Library
Chromium
Est
Microarray
Streptomycin
topic Cdna Library
Chromium
Est
Microarray
Streptomycin
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e- 10). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response.
Fil: Dos Santos Ferreira, Veronica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina
Fil: Rocchetta, Iara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Conforti, Visitacion Teresa D.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Bench, Shellie. University of California; Estados Unidos
Fil: Feldman, Robert. University of California; Estados Unidos. SymBio Corporation; Estados Unidos
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; Argentina. lnstitut Cochin; Francia
description To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value < e- 10). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p < 0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response.
publishDate 2007
dc.date.none.fl_str_mv 2007-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79885
Dos Santos Ferreira, Veronica Beatriz; Rocchetta, Iara; Conforti, Visitacion Teresa D.; Bench, Shellie; Feldman, Robert; et al.; Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress; Elsevier Science; Gene; 389; 2; 3-2007; 136-145
0378-1119
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79885
identifier_str_mv Dos Santos Ferreira, Veronica Beatriz; Rocchetta, Iara; Conforti, Visitacion Teresa D.; Bench, Shellie; Feldman, Robert; et al.; Gene expression patterns in Euglena gracilis: Insights into the cellular response to environmental stress; Elsevier Science; Gene; 389; 2; 3-2007; 136-145
0378-1119
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378111906006858
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2006.10.023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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