Lactococcus lactis strains delivering IL-10 protein or cDNA to the intestinal mucosa show anti-inflammatory properties in a TNBS-induced chronic colitis model

• Autores: del Carmen, Silvina Andrea; Martín, Rebeca; Saraiva, Tessalia; Zurita Turk, Meritxell; Miyoshi, Anderson; Azevedo, Vasco; Langella, Philippe; Bermúdez Humarán, Luis G.; de Moreno, Maria Alejandra; Leblanc, Jean Guy Joseph
• Año de publicación: 2014
• Idioma: inglés
• Tipo de recurso: documento de conferencia
• Materia: INTERLEUKIN-10; INFLAMMATORY BOWEL DISEASES; CDNA; COLITIS
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Interleukin-10 (IL-10) is the most important anti-inflammatory cytokine at intestinal level. Oral treatments with IL-10 are inefficient because of its sensitivity to the harsh conditions of the GI tract, and systemic treatments cause undesirable side effects. Our aim was to compare the protective effects of IL-10, delivered by recombinant lactoccoci using two novel expression systems, in a murine colitis model mimicking the relapsing nature of inflammatory bowel diseases. The first system is based on a stress-inducible promoter (pGroES/L) for the production and delivery of heterologous proteins in situ at mucosal surfaces, and the second allows the delivery to the host cells of an il-10 cDNA, harbored in a eukaryotic DNA expression vector (pValac). Colitis was induced in female BALB/c mice by intrarectal injection of TNBS. Mice that survived the challenge and recovered their body weight received one of the bacterial treatments or saline solution orally during 14 days. Colitis was reactivated 25 days after the first TNBS challenge with a second injection. Three days after colitis reactivation, cytokine profiles and inflammation in colon samples were evaluated. Animals receiving L. lactis delivering pGroES/L: il-10 or pValac: il-10 plasmids showed lower body weight loss and damage scores in their large intestines compared to inflamed non-treated mice. Both treatments also increased IL-10 concentration in the intestine, compared to the controls without treatment and maintained an increased ratio of IL-10/ pro-inflammatory cytokines. Our results confirm the protective effect of IL-10 delivered by L. lactis either as a protein or as a cDNA in a TNBS-induced chronic colitis model, and provides a step further in the use of genetically engineered bacteria as a valid system to deliver therapeutic molecules at mucosal level.
Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Martín, Rebeca. Institut National de la Recherche Agronomique; Francia
Fil: Saraiva, Tessalia. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Zurita Turk, Meritxell. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Miyoshi, Anderson. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia
Fil: Bermúdez Humarán, Luis G.. Institut National de la Recherche Agronomique; Francia
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
11th International Symposium on Lactic Acid Bacteria: Health, sustainability, diversity, and application
Egmond aan Zee
Países Bajos
Federation of European Microbiological Societies
Royal Netherlands Society for Microbiology