Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease

Autores
Leyria, Jimena; Guarneri, Alessandra A.; Lorenzo, Marcelo Gustavo; Nouzova, Marcela; Noriega, Fernando G.; Benrabaa, Samiha A. M.; Fernandez Lima, Francisco; Valadares Tose, Lilian; Orchard, Ian; Lange, Angela B.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The blood-sucking hemipteran Rhodnius prolixus is one of the main vectors of Chagas disease, a neglected tropical disease that affects several million people worldwide. Consuming a blood meal and mating are events with a high epidemiological impact since after each meal, mated females can lay fertile eggs that result in hundreds of offspring. Thus, a better knowledge of the control of R. prolixus reproductive capacity may provide targets for developing novel strategies to control vector populations, thereby reducing vector-host contacts and disease transmission. Here, we have used a combination of gene transcript expression analysis, biochemical assays, hormone measurements and studies of locomotory activity to investigate how mating influences egg development and egg laying rates in R. prolixus females. The results demonstrate that a blood meal increases egg production capacity and leads to earlier egg laying in mated females compared to virgins. Virgin females, however, have increased survival rate over mated females. Circulating juvenile hormone (JH) and ecdysteroid titers are increased in mated females, a process mainly driven through an upregulation of the transcripts for their biosynthetic enzymes in the corpus allatum and ovaries, respectively. Mated females display weaker locomotory activity compared to virgin females, mainly during the photophase. In essence, this study shows how reproductive output and behaviour are profoundly influenced by mating, highlighting molecular, biochemical, endocrine and behavioral features differentially expressed in mated and virgin R. prolixus females.
Fil: Leyria, Jimena. University of Toronto; Canadá
Fil: Guarneri, Alessandra A.. Instituto René Rachou; Brasil
Fil: Lorenzo, Marcelo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Nouzova, Marcela. Institute Of Parasitology; República Checa
Fil: Noriega, Fernando G.. Institute Of Parasitology; República Checa
Fil: Benrabaa, Samiha A. M.. University of Toronto; Canadá
Fil: Fernandez Lima, Francisco. Florida International University; Estados Unidos
Fil: Valadares Tose, Lilian. Florida International University; Estados Unidos
Fil: Orchard, Ian. University of Toronto; Canadá
Fil: Lange, Angela B.. University of Toronto; Canadá
Materia
Mating
Rhodnius
ovaries
Juvenile hormone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/250608
Acceder
id CONICETDig_fab0508ea0cc94ad3f94ea400d726b37
oai_identifier_str oai:ri.conicet.gov.ar:11336/250608
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas diseaseLeyria, JimenaGuarneri, Alessandra A.Lorenzo, Marcelo GustavoNouzova, MarcelaNoriega, Fernando G.Benrabaa, Samiha A. M.Fernandez Lima, FranciscoValadares Tose, LilianOrchard, IanLange, Angela B.MatingRhodniusovariesJuvenile hormonehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The blood-sucking hemipteran Rhodnius prolixus is one of the main vectors of Chagas disease, a neglected tropical disease that affects several million people worldwide. Consuming a blood meal and mating are events with a high epidemiological impact since after each meal, mated females can lay fertile eggs that result in hundreds of offspring. Thus, a better knowledge of the control of R. prolixus reproductive capacity may provide targets for developing novel strategies to control vector populations, thereby reducing vector-host contacts and disease transmission. Here, we have used a combination of gene transcript expression analysis, biochemical assays, hormone measurements and studies of locomotory activity to investigate how mating influences egg development and egg laying rates in R. prolixus females. The results demonstrate that a blood meal increases egg production capacity and leads to earlier egg laying in mated females compared to virgins. Virgin females, however, have increased survival rate over mated females. Circulating juvenile hormone (JH) and ecdysteroid titers are increased in mated females, a process mainly driven through an upregulation of the transcripts for their biosynthetic enzymes in the corpus allatum and ovaries, respectively. Mated females display weaker locomotory activity compared to virgin females, mainly during the photophase. In essence, this study shows how reproductive output and behaviour are profoundly influenced by mating, highlighting molecular, biochemical, endocrine and behavioral features differentially expressed in mated and virgin R. prolixus females.Fil: Leyria, Jimena. University of Toronto; CanadáFil: Guarneri, Alessandra A.. Instituto René Rachou; BrasilFil: Lorenzo, Marcelo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Nouzova, Marcela. Institute Of Parasitology; República ChecaFil: Noriega, Fernando G.. Institute Of Parasitology; República ChecaFil: Benrabaa, Samiha A. M.. University of Toronto; CanadáFil: Fernandez Lima, Francisco. Florida International University; Estados UnidosFil: Valadares Tose, Lilian. Florida International University; Estados UnidosFil: Orchard, Ian. University of Toronto; CanadáFil: Lange, Angela B.. University of Toronto; CanadáPublic Library of Science2023-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/250608Leyria, Jimena; Guarneri, Alessandra A.; Lorenzo, Marcelo Gustavo; Nouzova, Marcela; Noriega, Fernando G.; et al.; Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease; Public Library of Science; Neglected Tropical Diseases; 17; 9; 9-20231935-2735CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0011640info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0011640info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:36Zoai:ri.conicet.gov.ar:11336/250608instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:37.095CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
title Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
spellingShingle Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
Leyria, Jimena
Mating
Rhodnius
ovaries
Juvenile hormone
title_short Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
title_full Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
title_fullStr Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
title_full_unstemmed Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
title_sort Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease
dc.creator.none.fl_str_mv Leyria, Jimena
Guarneri, Alessandra A.
Lorenzo, Marcelo Gustavo
Nouzova, Marcela
Noriega, Fernando G.
Benrabaa, Samiha A. M.
Fernandez Lima, Francisco
Valadares Tose, Lilian
Orchard, Ian
Lange, Angela B.
author Leyria, Jimena
author_facet Leyria, Jimena
Guarneri, Alessandra A.
Lorenzo, Marcelo Gustavo
Nouzova, Marcela
Noriega, Fernando G.
Benrabaa, Samiha A. M.
Fernandez Lima, Francisco
Valadares Tose, Lilian
Orchard, Ian
Lange, Angela B.
author_role author
author2 Guarneri, Alessandra A.
Lorenzo, Marcelo Gustavo
Nouzova, Marcela
Noriega, Fernando G.
Benrabaa, Samiha A. M.
Fernandez Lima, Francisco
Valadares Tose, Lilian
Orchard, Ian
Lange, Angela B.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mating
Rhodnius
ovaries
Juvenile hormone
topic Mating
Rhodnius
ovaries
Juvenile hormone
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The blood-sucking hemipteran Rhodnius prolixus is one of the main vectors of Chagas disease, a neglected tropical disease that affects several million people worldwide. Consuming a blood meal and mating are events with a high epidemiological impact since after each meal, mated females can lay fertile eggs that result in hundreds of offspring. Thus, a better knowledge of the control of R. prolixus reproductive capacity may provide targets for developing novel strategies to control vector populations, thereby reducing vector-host contacts and disease transmission. Here, we have used a combination of gene transcript expression analysis, biochemical assays, hormone measurements and studies of locomotory activity to investigate how mating influences egg development and egg laying rates in R. prolixus females. The results demonstrate that a blood meal increases egg production capacity and leads to earlier egg laying in mated females compared to virgins. Virgin females, however, have increased survival rate over mated females. Circulating juvenile hormone (JH) and ecdysteroid titers are increased in mated females, a process mainly driven through an upregulation of the transcripts for their biosynthetic enzymes in the corpus allatum and ovaries, respectively. Mated females display weaker locomotory activity compared to virgin females, mainly during the photophase. In essence, this study shows how reproductive output and behaviour are profoundly influenced by mating, highlighting molecular, biochemical, endocrine and behavioral features differentially expressed in mated and virgin R. prolixus females.
Fil: Leyria, Jimena. University of Toronto; Canadá
Fil: Guarneri, Alessandra A.. Instituto René Rachou; Brasil
Fil: Lorenzo, Marcelo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Nouzova, Marcela. Institute Of Parasitology; República Checa
Fil: Noriega, Fernando G.. Institute Of Parasitology; República Checa
Fil: Benrabaa, Samiha A. M.. University of Toronto; Canadá
Fil: Fernandez Lima, Francisco. Florida International University; Estados Unidos
Fil: Valadares Tose, Lilian. Florida International University; Estados Unidos
Fil: Orchard, Ian. University of Toronto; Canadá
Fil: Lange, Angela B.. University of Toronto; Canadá
description The blood-sucking hemipteran Rhodnius prolixus is one of the main vectors of Chagas disease, a neglected tropical disease that affects several million people worldwide. Consuming a blood meal and mating are events with a high epidemiological impact since after each meal, mated females can lay fertile eggs that result in hundreds of offspring. Thus, a better knowledge of the control of R. prolixus reproductive capacity may provide targets for developing novel strategies to control vector populations, thereby reducing vector-host contacts and disease transmission. Here, we have used a combination of gene transcript expression analysis, biochemical assays, hormone measurements and studies of locomotory activity to investigate how mating influences egg development and egg laying rates in R. prolixus females. The results demonstrate that a blood meal increases egg production capacity and leads to earlier egg laying in mated females compared to virgins. Virgin females, however, have increased survival rate over mated females. Circulating juvenile hormone (JH) and ecdysteroid titers are increased in mated females, a process mainly driven through an upregulation of the transcripts for their biosynthetic enzymes in the corpus allatum and ovaries, respectively. Mated females display weaker locomotory activity compared to virgin females, mainly during the photophase. In essence, this study shows how reproductive output and behaviour are profoundly influenced by mating, highlighting molecular, biochemical, endocrine and behavioral features differentially expressed in mated and virgin R. prolixus females.
publishDate 2023
dc.date.none.fl_str_mv 2023-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/250608
Leyria, Jimena; Guarneri, Alessandra A.; Lorenzo, Marcelo Gustavo; Nouzova, Marcela; Noriega, Fernando G.; et al.; Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease; Public Library of Science; Neglected Tropical Diseases; 17; 9; 9-2023
1935-2735
CONICET Digital
CONICET
url http://hdl.handle.net/11336/250608
identifier_str_mv Leyria, Jimena; Guarneri, Alessandra A.; Lorenzo, Marcelo Gustavo; Nouzova, Marcela; Noriega, Fernando G.; et al.; Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease; Public Library of Science; Neglected Tropical Diseases; 17; 9; 9-2023
1935-2735
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0011640
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0011640
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613110881583104
score 13.070432

Publicaciones similares