The juvenile hormone described in <i>Rhodnius prolixus</i> by Wigglesworth is juvenile hormone III skipped bisepoxide

Autores
Villalobos Sambucaro, María José; Nouzova, Marcela; Ramirez, Cesar E.; Alzugaray, María Eugenia; Fernandez Lima, Francisco; Ronderos, Jorge Rafael; Noriega, Fernando G.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Juvenile hormones (JHs) are sesquiterpenoids synthesized by the corpora allata (CA). They play critical roles during insect development and reproduction. The first JH was described in 1934 as a "metamorphosis inhibitory hormone" in Rhodnius prolixus by Sir Vincent B. Wigglesworth. Remarkably, in spite of the importance of R. prolixus as vectors of Chagas disease and model organisms in insect physiology, the original JH that Wigglesworth described for the kissing-bug R. prolixus remained unidentified. We employed liquid chromatography mass spectrometry to search for the JH homologs present in the hemolymph of fourth instar nymphs of R. prolixus. Wigglesworth's original JH is the JH III skipped bisepoxide (JHSB3), a homolog identified in other heteropteran species. Changes in the titer of JHSB3 were studied during the 10-day long molting cycle of 4th instar nymph, between a blood meal and the ecdysis to 5th instar. In addition we measured the changes of mRNA levels in the CA for the 13 enzymes of the JH biosynthetic pathway during the molting cycle of 4th instar. Almost 90 years after the first descriptions of the role of JH in insects, this study finally reveals that the specific JH homolog responsible for Wigglesworth's original observations is JHSB3.
Facultad de Ciencias Naturales y Museo
Consejo Nacional de Investigaciones Científicas y Técnicas
Materia
Ciencias Naturales
Rhodnius
Hormonas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/123402
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/123402
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling The juvenile hormone described in <i>Rhodnius prolixus</i> by Wigglesworth is juvenile hormone III skipped bisepoxideVillalobos Sambucaro, María JoséNouzova, MarcelaRamirez, Cesar E.Alzugaray, María EugeniaFernandez Lima, FranciscoRonderos, Jorge RafaelNoriega, Fernando G.Ciencias NaturalesRhodniusHormonasJuvenile hormones (JHs) are sesquiterpenoids synthesized by the corpora allata (CA). They play critical roles during insect development and reproduction. The first JH was described in 1934 as a "metamorphosis inhibitory hormone" in Rhodnius prolixus by Sir Vincent B. Wigglesworth. Remarkably, in spite of the importance of R. prolixus as vectors of Chagas disease and model organisms in insect physiology, the original JH that Wigglesworth described for the kissing-bug R. prolixus remained unidentified. We employed liquid chromatography mass spectrometry to search for the JH homologs present in the hemolymph of fourth instar nymphs of R. prolixus. Wigglesworth's original JH is the JH III skipped bisepoxide (JHSB3), a homolog identified in other heteropteran species. Changes in the titer of JHSB3 were studied during the 10-day long molting cycle of 4th instar nymph, between a blood meal and the ecdysis to 5th instar. In addition we measured the changes of mRNA levels in the CA for the 13 enzymes of the JH biosynthetic pathway during the molting cycle of 4th instar. Almost 90 years after the first descriptions of the role of JH in insects, this study finally reveals that the specific JH homolog responsible for Wigglesworth's original observations is JHSB3.Facultad de Ciencias Naturales y MuseoConsejo Nacional de Investigaciones Científicas y Técnicas2020-02-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/123402enginfo:eu-repo/semantics/altIdentifier/issn/2045-2322info:eu-repo/semantics/altIdentifier/pmid/32080221info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-59495-1info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:24Zoai:sedici.unlp.edu.ar:10915/123402Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:24.697SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The juvenile hormone described in <i>Rhodnius prolixus</i> by Wigglesworth is juvenile hormone III skipped bisepoxide
title The juvenile hormone described in <i>Rhodnius prolixus</i> by Wigglesworth is juvenile hormone III skipped bisepoxide
spellingShingle The juvenile hormone described in <i>Rhodnius prolixus</i> by Wigglesworth is juvenile hormone III skipped bisepoxide
Villalobos Sambucaro, María José
Ciencias Naturales
Rhodnius
Hormonas
title_short The juvenile hormone described in <i>Rhodnius prolixus</i> by Wigglesworth is juvenile hormone III skipped bisepoxide
title_full The juvenile hormone described in <i>Rhodnius prolixus</i> by Wigglesworth is juvenile hormone III skipped bisepoxide
title_fullStr The juvenile hormone described in <i>Rhodnius prolixus</i> by Wigglesworth is juvenile hormone III skipped bisepoxide
title_full_unstemmed The juvenile hormone described in <i>Rhodnius prolixus</i> by Wigglesworth is juvenile hormone III skipped bisepoxide
title_sort The juvenile hormone described in <i>Rhodnius prolixus</i> by Wigglesworth is juvenile hormone III skipped bisepoxide
dc.creator.none.fl_str_mv Villalobos Sambucaro, María José
Nouzova, Marcela
Ramirez, Cesar E.
Alzugaray, María Eugenia
Fernandez Lima, Francisco
Ronderos, Jorge Rafael
Noriega, Fernando G.
author Villalobos Sambucaro, María José
author_facet Villalobos Sambucaro, María José
Nouzova, Marcela
Ramirez, Cesar E.
Alzugaray, María Eugenia
Fernandez Lima, Francisco
Ronderos, Jorge Rafael
Noriega, Fernando G.
author_role author
author2 Nouzova, Marcela
Ramirez, Cesar E.
Alzugaray, María Eugenia
Fernandez Lima, Francisco
Ronderos, Jorge Rafael
Noriega, Fernando G.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Rhodnius
Hormonas
topic Ciencias Naturales
Rhodnius
Hormonas
dc.description.none.fl_txt_mv Juvenile hormones (JHs) are sesquiterpenoids synthesized by the corpora allata (CA). They play critical roles during insect development and reproduction. The first JH was described in 1934 as a "metamorphosis inhibitory hormone" in Rhodnius prolixus by Sir Vincent B. Wigglesworth. Remarkably, in spite of the importance of R. prolixus as vectors of Chagas disease and model organisms in insect physiology, the original JH that Wigglesworth described for the kissing-bug R. prolixus remained unidentified. We employed liquid chromatography mass spectrometry to search for the JH homologs present in the hemolymph of fourth instar nymphs of R. prolixus. Wigglesworth's original JH is the JH III skipped bisepoxide (JHSB3), a homolog identified in other heteropteran species. Changes in the titer of JHSB3 were studied during the 10-day long molting cycle of 4th instar nymph, between a blood meal and the ecdysis to 5th instar. In addition we measured the changes of mRNA levels in the CA for the 13 enzymes of the JH biosynthetic pathway during the molting cycle of 4th instar. Almost 90 years after the first descriptions of the role of JH in insects, this study finally reveals that the specific JH homolog responsible for Wigglesworth's original observations is JHSB3.
Facultad de Ciencias Naturales y Museo
Consejo Nacional de Investigaciones Científicas y Técnicas
description Juvenile hormones (JHs) are sesquiterpenoids synthesized by the corpora allata (CA). They play critical roles during insect development and reproduction. The first JH was described in 1934 as a "metamorphosis inhibitory hormone" in Rhodnius prolixus by Sir Vincent B. Wigglesworth. Remarkably, in spite of the importance of R. prolixus as vectors of Chagas disease and model organisms in insect physiology, the original JH that Wigglesworth described for the kissing-bug R. prolixus remained unidentified. We employed liquid chromatography mass spectrometry to search for the JH homologs present in the hemolymph of fourth instar nymphs of R. prolixus. Wigglesworth's original JH is the JH III skipped bisepoxide (JHSB3), a homolog identified in other heteropteran species. Changes in the titer of JHSB3 were studied during the 10-day long molting cycle of 4th instar nymph, between a blood meal and the ecdysis to 5th instar. In addition we measured the changes of mRNA levels in the CA for the 13 enzymes of the JH biosynthetic pathway during the molting cycle of 4th instar. Almost 90 years after the first descriptions of the role of JH in insects, this study finally reveals that the specific JH homolog responsible for Wigglesworth's original observations is JHSB3.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/123402
url http://sedici.unlp.edu.ar/handle/10915/123402
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2045-2322
info:eu-repo/semantics/altIdentifier/pmid/32080221
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-59495-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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