Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries

Autores
García, S.; Nanfaro, F.; Casas, Sebastián Marcelo; Cabrera Kreiker, Ricardo Jorge; Yunes, Roberto Miguel Federico
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The reagent MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole) is reduced to formazan in living cells, giving a purple colored product. The product absorbance issusceptible to be measured at a certain wavelength by a spectrophotometer. We report in this paper the use of MTT as a suitable method for checking both the viability of neural tissues as well as the correct location of stereotaxically placed cerebral cannulae. Evidence is given that this method can be useful not only because it is a new way of utilizing a vital staining to evaluate cannulae location, but also because MTT is a simple, effective and cheaper way of assessing the arousal level of neural tissues under study. In doing so, we can effectively test the stimulatory and/or inhibitory actions of several reagents in what we finally named as MTT-micro assay on living tissues: the MTT-malt.
Fil: García, S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Nanfaro, F.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Materia
Neurodegenerative Disease
Animal Models
Viability
Mtt
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/81179
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/81179
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic SurgeriesGarcía, S.Nanfaro, F.Casas, Sebastián MarceloCabrera Kreiker, Ricardo JorgeYunes, Roberto Miguel FedericoNeurodegenerative DiseaseAnimal ModelsViabilityMtthttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The reagent MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole) is reduced to formazan in living cells, giving a purple colored product. The product absorbance issusceptible to be measured at a certain wavelength by a spectrophotometer. We report in this paper the use of MTT as a suitable method for checking both the viability of neural tissues as well as the correct location of stereotaxically placed cerebral cannulae. Evidence is given that this method can be useful not only because it is a new way of utilizing a vital staining to evaluate cannulae location, but also because MTT is a simple, effective and cheaper way of assessing the arousal level of neural tissues under study. In doing so, we can effectively test the stimulatory and/or inhibitory actions of several reagents in what we finally named as MTT-micro assay on living tissues: the MTT-malt.Fil: García, S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Nanfaro, F.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaAmerican Scientific Publishers2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/81179García, S.; Nanfaro, F.; Casas, Sebastián Marcelo; Cabrera Kreiker, Ricardo Jorge; Yunes, Roberto Miguel Federico; Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries; American Scientific Publishers; American Journal of Neuroprotection and Neuroregeneration; 4; 1; 10-2012; 58-621947-29511947-296XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1166/ajnn.2012.1037info:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/asp/ajnn/2012/00000004/00000001/art00008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:19Zoai:ri.conicet.gov.ar:11336/81179instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:20.155CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries
title Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries
spellingShingle Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries
García, S.
Neurodegenerative Disease
Animal Models
Viability
Mtt
title_short Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries
title_full Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries
title_fullStr Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries
title_full_unstemmed Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries
title_sort Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries
dc.creator.none.fl_str_mv García, S.
Nanfaro, F.
Casas, Sebastián Marcelo
Cabrera Kreiker, Ricardo Jorge
Yunes, Roberto Miguel Federico
author García, S.
author_facet García, S.
Nanfaro, F.
Casas, Sebastián Marcelo
Cabrera Kreiker, Ricardo Jorge
Yunes, Roberto Miguel Federico
author_role author
author2 Nanfaro, F.
Casas, Sebastián Marcelo
Cabrera Kreiker, Ricardo Jorge
Yunes, Roberto Miguel Federico
author2_role author
author
author
author
dc.subject.none.fl_str_mv Neurodegenerative Disease
Animal Models
Viability
Mtt
topic Neurodegenerative Disease
Animal Models
Viability
Mtt
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The reagent MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole) is reduced to formazan in living cells, giving a purple colored product. The product absorbance issusceptible to be measured at a certain wavelength by a spectrophotometer. We report in this paper the use of MTT as a suitable method for checking both the viability of neural tissues as well as the correct location of stereotaxically placed cerebral cannulae. Evidence is given that this method can be useful not only because it is a new way of utilizing a vital staining to evaluate cannulae location, but also because MTT is a simple, effective and cheaper way of assessing the arousal level of neural tissues under study. In doing so, we can effectively test the stimulatory and/or inhibitory actions of several reagents in what we finally named as MTT-micro assay on living tissues: the MTT-malt.
Fil: García, S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Nanfaro, F.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
description The reagent MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole) is reduced to formazan in living cells, giving a purple colored product. The product absorbance issusceptible to be measured at a certain wavelength by a spectrophotometer. We report in this paper the use of MTT as a suitable method for checking both the viability of neural tissues as well as the correct location of stereotaxically placed cerebral cannulae. Evidence is given that this method can be useful not only because it is a new way of utilizing a vital staining to evaluate cannulae location, but also because MTT is a simple, effective and cheaper way of assessing the arousal level of neural tissues under study. In doing so, we can effectively test the stimulatory and/or inhibitory actions of several reagents in what we finally named as MTT-micro assay on living tissues: the MTT-malt.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/81179
García, S.; Nanfaro, F.; Casas, Sebastián Marcelo; Cabrera Kreiker, Ricardo Jorge; Yunes, Roberto Miguel Federico; Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries; American Scientific Publishers; American Journal of Neuroprotection and Neuroregeneration; 4; 1; 10-2012; 58-62
1947-2951
1947-296X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/81179
identifier_str_mv García, S.; Nanfaro, F.; Casas, Sebastián Marcelo; Cabrera Kreiker, Ricardo Jorge; Yunes, Roberto Miguel Federico; Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries; American Scientific Publishers; American Journal of Neuroprotection and Neuroregeneration; 4; 1; 10-2012; 58-62
1947-2951
1947-296X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1166/ajnn.2012.1037
info:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/asp/ajnn/2012/00000004/00000001/art00008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Scientific Publishers
publisher.none.fl_str_mv American Scientific Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613650744082432
score 13.069144

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