Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers

Autores
Da Cruz Cabral, Lucía Mariana; Terminiello, Laura Adriana; Fernández Pinto, Virginia; Nielsen, Kristian Fog; Patriarca, Andrea Rosana
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sweet pepper (Capsicum annuum) is an important crop cultivated worldwide, with Argentina being one of the major producers in South America. The fruit is susceptible to several fungal diseases, leading to severe economic losses for producers. In this study, Alternaria was found as the prevalent genus in mouldy peppers (50% fruits infected). Morphological identification revealed that all 64 Alternaria isolates belonged to small-spored species, most of them corresponding to A. tenuissima, A. arborescens and A. alternata species-groups. Their secondary metabolite profile was evaluated in vitro; alternariols were synthesized by most of the isolates (91% for alternariol and 92% for alternariol monomethyl ether). A high number of Alternaria spp. also produced tenuazonic acid (64%), altenuene (84%) and tentoxin (72%). In addition, damaged pepper fruits were analysed for the presence of tenuazonic acid and alternariols. A total 32 out of 48 spoiled pepper fruits were contaminated with at least one of these metabolites. Half of the samples were positive for tenuazonic acid (range 8–11,422 μg/kg), while alternariol and its monomethyl ether were less frequently detected (21 and 29%, respectively) and at lower concentrations. This is the first report on the natural occurrence of Alternaria mycotoxins in Argentinean sweet pepper, and highlights a consumer risk when mouldy fruits are used in industrialized products because these compounds are not destroyed by conventional heat treatments.
Fil: Da Cruz Cabral, Lucía Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Terminiello, Laura Adriana. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Fernández Pinto, Virginia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Nielsen, Kristian Fog. Technical University of Denmark; Dinamarca
Fil: Patriarca, Andrea Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Materia
Alternaria
Alternariols
Mycota
Pepper Fruits
Secondary Metabolites
Tenuazonic Acid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26484
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/26484
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppersDa Cruz Cabral, Lucía MarianaTerminiello, Laura AdrianaFernández Pinto, VirginiaNielsen, Kristian FogPatriarca, Andrea RosanaAlternariaAlternariolsMycotaPepper FruitsSecondary MetabolitesTenuazonic Acidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sweet pepper (Capsicum annuum) is an important crop cultivated worldwide, with Argentina being one of the major producers in South America. The fruit is susceptible to several fungal diseases, leading to severe economic losses for producers. In this study, Alternaria was found as the prevalent genus in mouldy peppers (50% fruits infected). Morphological identification revealed that all 64 Alternaria isolates belonged to small-spored species, most of them corresponding to A. tenuissima, A. arborescens and A. alternata species-groups. Their secondary metabolite profile was evaluated in vitro; alternariols were synthesized by most of the isolates (91% for alternariol and 92% for alternariol monomethyl ether). A high number of Alternaria spp. also produced tenuazonic acid (64%), altenuene (84%) and tentoxin (72%). In addition, damaged pepper fruits were analysed for the presence of tenuazonic acid and alternariols. A total 32 out of 48 spoiled pepper fruits were contaminated with at least one of these metabolites. Half of the samples were positive for tenuazonic acid (range 8–11,422 μg/kg), while alternariol and its monomethyl ether were less frequently detected (21 and 29%, respectively) and at lower concentrations. This is the first report on the natural occurrence of Alternaria mycotoxins in Argentinean sweet pepper, and highlights a consumer risk when mouldy fruits are used in industrialized products because these compounds are not destroyed by conventional heat treatments.Fil: Da Cruz Cabral, Lucía Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Terminiello, Laura Adriana. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Fernández Pinto, Virginia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Nielsen, Kristian Fog. Technical University of Denmark; DinamarcaFil: Patriarca, Andrea Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaElsevier Science2016-11-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26484Da Cruz Cabral, Lucía Mariana; Terminiello, Laura Adriana; Fernández Pinto, Virginia; Nielsen, Kristian Fog; Patriarca, Andrea Rosana; Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers; Elsevier Science; International Journal of Food Microbiology; 236; 4-11-2016; 155-1600168-16051879-3460CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168160516303981info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2016.08.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:57Zoai:ri.conicet.gov.ar:11336/26484instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:58.166CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers
title Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers
spellingShingle Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers
Da Cruz Cabral, Lucía Mariana
Alternaria
Alternariols
Mycota
Pepper Fruits
Secondary Metabolites
Tenuazonic Acid
title_short Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers
title_full Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers
title_fullStr Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers
title_full_unstemmed Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers
title_sort Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers
dc.creator.none.fl_str_mv Da Cruz Cabral, Lucía Mariana
Terminiello, Laura Adriana
Fernández Pinto, Virginia
Nielsen, Kristian Fog
Patriarca, Andrea Rosana
author Da Cruz Cabral, Lucía Mariana
author_facet Da Cruz Cabral, Lucía Mariana
Terminiello, Laura Adriana
Fernández Pinto, Virginia
Nielsen, Kristian Fog
Patriarca, Andrea Rosana
author_role author
author2 Terminiello, Laura Adriana
Fernández Pinto, Virginia
Nielsen, Kristian Fog
Patriarca, Andrea Rosana
author2_role author
author
author
author
dc.subject.none.fl_str_mv Alternaria
Alternariols
Mycota
Pepper Fruits
Secondary Metabolites
Tenuazonic Acid
topic Alternaria
Alternariols
Mycota
Pepper Fruits
Secondary Metabolites
Tenuazonic Acid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sweet pepper (Capsicum annuum) is an important crop cultivated worldwide, with Argentina being one of the major producers in South America. The fruit is susceptible to several fungal diseases, leading to severe economic losses for producers. In this study, Alternaria was found as the prevalent genus in mouldy peppers (50% fruits infected). Morphological identification revealed that all 64 Alternaria isolates belonged to small-spored species, most of them corresponding to A. tenuissima, A. arborescens and A. alternata species-groups. Their secondary metabolite profile was evaluated in vitro; alternariols were synthesized by most of the isolates (91% for alternariol and 92% for alternariol monomethyl ether). A high number of Alternaria spp. also produced tenuazonic acid (64%), altenuene (84%) and tentoxin (72%). In addition, damaged pepper fruits were analysed for the presence of tenuazonic acid and alternariols. A total 32 out of 48 spoiled pepper fruits were contaminated with at least one of these metabolites. Half of the samples were positive for tenuazonic acid (range 8–11,422 μg/kg), while alternariol and its monomethyl ether were less frequently detected (21 and 29%, respectively) and at lower concentrations. This is the first report on the natural occurrence of Alternaria mycotoxins in Argentinean sweet pepper, and highlights a consumer risk when mouldy fruits are used in industrialized products because these compounds are not destroyed by conventional heat treatments.
Fil: Da Cruz Cabral, Lucía Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Terminiello, Laura Adriana. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Fernández Pinto, Virginia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Nielsen, Kristian Fog. Technical University of Denmark; Dinamarca
Fil: Patriarca, Andrea Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
description Sweet pepper (Capsicum annuum) is an important crop cultivated worldwide, with Argentina being one of the major producers in South America. The fruit is susceptible to several fungal diseases, leading to severe economic losses for producers. In this study, Alternaria was found as the prevalent genus in mouldy peppers (50% fruits infected). Morphological identification revealed that all 64 Alternaria isolates belonged to small-spored species, most of them corresponding to A. tenuissima, A. arborescens and A. alternata species-groups. Their secondary metabolite profile was evaluated in vitro; alternariols were synthesized by most of the isolates (91% for alternariol and 92% for alternariol monomethyl ether). A high number of Alternaria spp. also produced tenuazonic acid (64%), altenuene (84%) and tentoxin (72%). In addition, damaged pepper fruits were analysed for the presence of tenuazonic acid and alternariols. A total 32 out of 48 spoiled pepper fruits were contaminated with at least one of these metabolites. Half of the samples were positive for tenuazonic acid (range 8–11,422 μg/kg), while alternariol and its monomethyl ether were less frequently detected (21 and 29%, respectively) and at lower concentrations. This is the first report on the natural occurrence of Alternaria mycotoxins in Argentinean sweet pepper, and highlights a consumer risk when mouldy fruits are used in industrialized products because these compounds are not destroyed by conventional heat treatments.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26484
Da Cruz Cabral, Lucía Mariana; Terminiello, Laura Adriana; Fernández Pinto, Virginia; Nielsen, Kristian Fog; Patriarca, Andrea Rosana; Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers; Elsevier Science; International Journal of Food Microbiology; 236; 4-11-2016; 155-160
0168-1605
1879-3460
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26484
identifier_str_mv Da Cruz Cabral, Lucía Mariana; Terminiello, Laura Adriana; Fernández Pinto, Virginia; Nielsen, Kristian Fog; Patriarca, Andrea Rosana; Natural occurrence of mycotoxins and toxigenic capacity of Alternaria strains from mouldy peppers; Elsevier Science; International Journal of Food Microbiology; 236; 4-11-2016; 155-160
0168-1605
1879-3460
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168160516303981
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2016.08.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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