Toxigenic Alternaria species from Argentinean blueberries

Autores
Greco, Mariana Vanesa; Patriarca, A.; Terminiello, Laura Adriana; Fernández Pinto, Virginia Elena; Pose, Graciela Noemi
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Blueberries are traditionally consumed in North America, some European countries and Japan. In Argentina, the blueberry crop is profitable because production starts in November, when the northern hemisphere lacks fresh fruit. Fungal contaminants can grow and produce mycotoxins in fresh fruit. The aims of this work were to identify the main genera of the mycobiota of blueberries grown in Argentina and to determine the toxicogenic potential, pathogenicity and host specificity of the species isolated. The genus Alternaria was the main component of the blueberry mycobiota (95%); minor proportions of Phoma spp. (4%) and Penicillium spp. (1%) were also isolated. According to their sporulation patterns, 127 Alternaria isolates belonged to the Alternaria tenuissima species-group, 5 to the Alternaria alternata species-group and 2 to the Alternaria arborescens species-group. The last mentioned species-group was not isolated at 5. °C. Of the 134 isolates, 61% were toxicogenic in autoclaved rice; 97% of these produced alternariol (AOH) in a range from 0.14 to 119.18. mg/kg, 95% produced alternariol methylether (AME) in a range from 1.23 to 901.74. mg/kg and 65% produced tenuazonic acid (TA) in a range from 0.13 to 2778. mg/kg. Fifty two isolates co-produced the three mycotoxins. According to the size of the lesion that they caused on blueberries, the isolates were classified as slightly pathogenic, moderately pathogenic and very pathogenic. No significant differences in pathogenicity were found on different blueberry varieties.In this work, high incidence and toxicogenic potential of the Alternaria isolates from blueberries were demonstrated. Thus, more studies should be done to evaluate the health risk posed by the presence of the Alternaria toxins in blueberries and in the manufactured by-products.
Fil: Greco, Mariana Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Área Ingeniería en Alimentos; Argentina
Fil: Patriarca, A.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; Argentina
Fil: Terminiello, Laura Adriana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández Pinto, Virginia Elena. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pose, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Área Ingeniería en Alimentos; Argentina
Materia
ALTERNARIA
ALTERNARIOL
ALTERNARIOL METHYLETHER
BLUEBERRIES
TENUAZONIC ACID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194576
Acceder
id CONICETDig_4af813703cdec204e65b73e60ae0c7ec
oai_identifier_str oai:ri.conicet.gov.ar:11336/194576
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Toxigenic Alternaria species from Argentinean blueberriesGreco, Mariana VanesaPatriarca, A.Terminiello, Laura AdrianaFernández Pinto, Virginia ElenaPose, Graciela NoemiALTERNARIAALTERNARIOLALTERNARIOL METHYLETHERBLUEBERRIESTENUAZONIC ACIDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Blueberries are traditionally consumed in North America, some European countries and Japan. In Argentina, the blueberry crop is profitable because production starts in November, when the northern hemisphere lacks fresh fruit. Fungal contaminants can grow and produce mycotoxins in fresh fruit. The aims of this work were to identify the main genera of the mycobiota of blueberries grown in Argentina and to determine the toxicogenic potential, pathogenicity and host specificity of the species isolated. The genus Alternaria was the main component of the blueberry mycobiota (95%); minor proportions of Phoma spp. (4%) and Penicillium spp. (1%) were also isolated. According to their sporulation patterns, 127 Alternaria isolates belonged to the Alternaria tenuissima species-group, 5 to the Alternaria alternata species-group and 2 to the Alternaria arborescens species-group. The last mentioned species-group was not isolated at 5. °C. Of the 134 isolates, 61% were toxicogenic in autoclaved rice; 97% of these produced alternariol (AOH) in a range from 0.14 to 119.18. mg/kg, 95% produced alternariol methylether (AME) in a range from 1.23 to 901.74. mg/kg and 65% produced tenuazonic acid (TA) in a range from 0.13 to 2778. mg/kg. Fifty two isolates co-produced the three mycotoxins. According to the size of the lesion that they caused on blueberries, the isolates were classified as slightly pathogenic, moderately pathogenic and very pathogenic. No significant differences in pathogenicity were found on different blueberry varieties.In this work, high incidence and toxicogenic potential of the Alternaria isolates from blueberries were demonstrated. Thus, more studies should be done to evaluate the health risk posed by the presence of the Alternaria toxins in blueberries and in the manufactured by-products.Fil: Greco, Mariana Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Área Ingeniería en Alimentos; ArgentinaFil: Patriarca, A.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; ArgentinaFil: Terminiello, Laura Adriana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández Pinto, Virginia Elena. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pose, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Área Ingeniería en Alimentos; ArgentinaElsevier Science2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194576Greco, Mariana Vanesa; Patriarca, A.; Terminiello, Laura Adriana; Fernández Pinto, Virginia Elena; Pose, Graciela Noemi; Toxigenic Alternaria species from Argentinean blueberries; Elsevier Science; International Journal of Food Microbiology; 154; 3; 1-2012; 187-1910168-1605CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168160512000074info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2012.01.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:14:47Zoai:ri.conicet.gov.ar:11336/194576instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:14:48.117CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Toxigenic Alternaria species from Argentinean blueberries
title Toxigenic Alternaria species from Argentinean blueberries
spellingShingle Toxigenic Alternaria species from Argentinean blueberries
Greco, Mariana Vanesa
ALTERNARIA
ALTERNARIOL
ALTERNARIOL METHYLETHER
BLUEBERRIES
TENUAZONIC ACID
title_short Toxigenic Alternaria species from Argentinean blueberries
title_full Toxigenic Alternaria species from Argentinean blueberries
title_fullStr Toxigenic Alternaria species from Argentinean blueberries
title_full_unstemmed Toxigenic Alternaria species from Argentinean blueberries
title_sort Toxigenic Alternaria species from Argentinean blueberries
dc.creator.none.fl_str_mv Greco, Mariana Vanesa
Patriarca, A.
Terminiello, Laura Adriana
Fernández Pinto, Virginia Elena
Pose, Graciela Noemi
author Greco, Mariana Vanesa
author_facet Greco, Mariana Vanesa
Patriarca, A.
Terminiello, Laura Adriana
Fernández Pinto, Virginia Elena
Pose, Graciela Noemi
author_role author
author2 Patriarca, A.
Terminiello, Laura Adriana
Fernández Pinto, Virginia Elena
Pose, Graciela Noemi
author2_role author
author
author
author
dc.subject.none.fl_str_mv ALTERNARIA
ALTERNARIOL
ALTERNARIOL METHYLETHER
BLUEBERRIES
TENUAZONIC ACID
topic ALTERNARIA
ALTERNARIOL
ALTERNARIOL METHYLETHER
BLUEBERRIES
TENUAZONIC ACID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Blueberries are traditionally consumed in North America, some European countries and Japan. In Argentina, the blueberry crop is profitable because production starts in November, when the northern hemisphere lacks fresh fruit. Fungal contaminants can grow and produce mycotoxins in fresh fruit. The aims of this work were to identify the main genera of the mycobiota of blueberries grown in Argentina and to determine the toxicogenic potential, pathogenicity and host specificity of the species isolated. The genus Alternaria was the main component of the blueberry mycobiota (95%); minor proportions of Phoma spp. (4%) and Penicillium spp. (1%) were also isolated. According to their sporulation patterns, 127 Alternaria isolates belonged to the Alternaria tenuissima species-group, 5 to the Alternaria alternata species-group and 2 to the Alternaria arborescens species-group. The last mentioned species-group was not isolated at 5. °C. Of the 134 isolates, 61% were toxicogenic in autoclaved rice; 97% of these produced alternariol (AOH) in a range from 0.14 to 119.18. mg/kg, 95% produced alternariol methylether (AME) in a range from 1.23 to 901.74. mg/kg and 65% produced tenuazonic acid (TA) in a range from 0.13 to 2778. mg/kg. Fifty two isolates co-produced the three mycotoxins. According to the size of the lesion that they caused on blueberries, the isolates were classified as slightly pathogenic, moderately pathogenic and very pathogenic. No significant differences in pathogenicity were found on different blueberry varieties.In this work, high incidence and toxicogenic potential of the Alternaria isolates from blueberries were demonstrated. Thus, more studies should be done to evaluate the health risk posed by the presence of the Alternaria toxins in blueberries and in the manufactured by-products.
Fil: Greco, Mariana Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Área Ingeniería en Alimentos; Argentina
Fil: Patriarca, A.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; Argentina
Fil: Terminiello, Laura Adriana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández Pinto, Virginia Elena. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pose, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Área Ingeniería en Alimentos; Argentina
description Blueberries are traditionally consumed in North America, some European countries and Japan. In Argentina, the blueberry crop is profitable because production starts in November, when the northern hemisphere lacks fresh fruit. Fungal contaminants can grow and produce mycotoxins in fresh fruit. The aims of this work were to identify the main genera of the mycobiota of blueberries grown in Argentina and to determine the toxicogenic potential, pathogenicity and host specificity of the species isolated. The genus Alternaria was the main component of the blueberry mycobiota (95%); minor proportions of Phoma spp. (4%) and Penicillium spp. (1%) were also isolated. According to their sporulation patterns, 127 Alternaria isolates belonged to the Alternaria tenuissima species-group, 5 to the Alternaria alternata species-group and 2 to the Alternaria arborescens species-group. The last mentioned species-group was not isolated at 5. °C. Of the 134 isolates, 61% were toxicogenic in autoclaved rice; 97% of these produced alternariol (AOH) in a range from 0.14 to 119.18. mg/kg, 95% produced alternariol methylether (AME) in a range from 1.23 to 901.74. mg/kg and 65% produced tenuazonic acid (TA) in a range from 0.13 to 2778. mg/kg. Fifty two isolates co-produced the three mycotoxins. According to the size of the lesion that they caused on blueberries, the isolates were classified as slightly pathogenic, moderately pathogenic and very pathogenic. No significant differences in pathogenicity were found on different blueberry varieties.In this work, high incidence and toxicogenic potential of the Alternaria isolates from blueberries were demonstrated. Thus, more studies should be done to evaluate the health risk posed by the presence of the Alternaria toxins in blueberries and in the manufactured by-products.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194576
Greco, Mariana Vanesa; Patriarca, A.; Terminiello, Laura Adriana; Fernández Pinto, Virginia Elena; Pose, Graciela Noemi; Toxigenic Alternaria species from Argentinean blueberries; Elsevier Science; International Journal of Food Microbiology; 154; 3; 1-2012; 187-191
0168-1605
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194576
identifier_str_mv Greco, Mariana Vanesa; Patriarca, A.; Terminiello, Laura Adriana; Fernández Pinto, Virginia Elena; Pose, Graciela Noemi; Toxigenic Alternaria species from Argentinean blueberries; Elsevier Science; International Journal of Food Microbiology; 154; 3; 1-2012; 187-191
0168-1605
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168160512000074
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2012.01.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083295572393984
score 13.221938

Publicaciones similares