Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina

Autores
Prendes, Luciana Paola; Merín, María Gabriela; Andreoni, Mario A.; Ramirez, Maria Laura; Morata, Vilma Ines
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Alternaria, one of the most mycotoxigenic genus commonly found in wine grapes, could represent a high risk for wine consumer's health. The aims of this work were to identify the mycobiota of Malbec wine grapes under the influence of routine control viticulture practices, to identify Alternaria spp. strains by morphological and molecular methods and characterize their toxicogenic ability and pathogenicity. Alternaria was the main component of the wine grape mycobiota of the DOC San Rafael at harvest time (81%) followed by Cladosporium (7%) and only in minor percentage by Penicillium (4%) and Aspergillus (3%) among others. The application of an organic or non-organic treatment in the vineyard did not affect significantly the incidence of the present genera. According to morphological and molecular identification based on the genetic marker Alt a 1, all Alternaria isolates were included into Alternaria alternata species. Of 34 analyzed Alternaria strains, 97% were able to produce at least one of the three mycotoxins analyzed: alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TA) and 53% co-produced the three mycotoxins. TA was the toxin produced at highest frequency (97%) and at highest levels in a range from 11.2 to 1941.0ppm. It was followed by AOH produced by 71% of the strains, in a range from 1.8 to 437.0ppm and AME produced by 59% of the strains, in a range from 0.6ppm to 663.4ppm. The 55% of the Alternaria strains were very pathogenic, 31% moderately pathogenic and only 14% were slightly pathogenic. In the present work, a high incidence and prevalence of Alternaria genus was reported despite the use of routine control viticulture practices, as well as a high percentage of toxicogenic and pathogenic Alternaria strains.
Fil: Prendes, Luciana Paola. Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Merín, María Gabriela. Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Andreoni, Mario A.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Ramirez, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Centro de Investigaciones Cientificas ; Facultad de Ciencias Exactas Fisicas y Naturales ; Universidad Nacional de Cordoba;
Fil: Morata, Vilma Ines. Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Materia
ALTERNARIA
ALTERNARIOL
ALTERNARIOL MONOMETHYL ETHER
TENUAZONIC ACID
WINE GRAPES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180196
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/180196
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, ArgentinaPrendes, Luciana PaolaMerín, María GabrielaAndreoni, Mario A.Ramirez, Maria LauraMorata, Vilma InesALTERNARIAALTERNARIOLALTERNARIOL MONOMETHYL ETHERTENUAZONIC ACIDWINE GRAPEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Alternaria, one of the most mycotoxigenic genus commonly found in wine grapes, could represent a high risk for wine consumer's health. The aims of this work were to identify the mycobiota of Malbec wine grapes under the influence of routine control viticulture practices, to identify Alternaria spp. strains by morphological and molecular methods and characterize their toxicogenic ability and pathogenicity. Alternaria was the main component of the wine grape mycobiota of the DOC San Rafael at harvest time (81%) followed by Cladosporium (7%) and only in minor percentage by Penicillium (4%) and Aspergillus (3%) among others. The application of an organic or non-organic treatment in the vineyard did not affect significantly the incidence of the present genera. According to morphological and molecular identification based on the genetic marker Alt a 1, all Alternaria isolates were included into Alternaria alternata species. Of 34 analyzed Alternaria strains, 97% were able to produce at least one of the three mycotoxins analyzed: alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TA) and 53% co-produced the three mycotoxins. TA was the toxin produced at highest frequency (97%) and at highest levels in a range from 11.2 to 1941.0ppm. It was followed by AOH produced by 71% of the strains, in a range from 1.8 to 437.0ppm and AME produced by 59% of the strains, in a range from 0.6ppm to 663.4ppm. The 55% of the Alternaria strains were very pathogenic, 31% moderately pathogenic and only 14% were slightly pathogenic. In the present work, a high incidence and prevalence of Alternaria genus was reported despite the use of routine control viticulture practices, as well as a high percentage of toxicogenic and pathogenic Alternaria strains.Fil: Prendes, Luciana Paola. Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaFil: Merín, María Gabriela. Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaFil: Andreoni, Mario A.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Ramirez, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Centro de Investigaciones Cientificas ; Facultad de Ciencias Exactas Fisicas y Naturales ; Universidad Nacional de Cordoba;Fil: Morata, Vilma Ines. Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaElsevier2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180196Prendes, Luciana Paola; Merín, María Gabriela; Andreoni, Mario A.; Ramirez, Maria Laura; Morata, Vilma Ines; Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina; Elsevier; Food Control; 57; 3-2015; 122-1280956-7135CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0956713515002169info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodcont.2015.03.041info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:28Zoai:ri.conicet.gov.ar:11336/180196instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:29.158CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina
title Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina
spellingShingle Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina
Prendes, Luciana Paola
ALTERNARIA
ALTERNARIOL
ALTERNARIOL MONOMETHYL ETHER
TENUAZONIC ACID
WINE GRAPES
title_short Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina
title_full Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina
title_fullStr Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina
title_full_unstemmed Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina
title_sort Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina
dc.creator.none.fl_str_mv Prendes, Luciana Paola
Merín, María Gabriela
Andreoni, Mario A.
Ramirez, Maria Laura
Morata, Vilma Ines
author Prendes, Luciana Paola
author_facet Prendes, Luciana Paola
Merín, María Gabriela
Andreoni, Mario A.
Ramirez, Maria Laura
Morata, Vilma Ines
author_role author
author2 Merín, María Gabriela
Andreoni, Mario A.
Ramirez, Maria Laura
Morata, Vilma Ines
author2_role author
author
author
author
dc.subject.none.fl_str_mv ALTERNARIA
ALTERNARIOL
ALTERNARIOL MONOMETHYL ETHER
TENUAZONIC ACID
WINE GRAPES
topic ALTERNARIA
ALTERNARIOL
ALTERNARIOL MONOMETHYL ETHER
TENUAZONIC ACID
WINE GRAPES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Alternaria, one of the most mycotoxigenic genus commonly found in wine grapes, could represent a high risk for wine consumer's health. The aims of this work were to identify the mycobiota of Malbec wine grapes under the influence of routine control viticulture practices, to identify Alternaria spp. strains by morphological and molecular methods and characterize their toxicogenic ability and pathogenicity. Alternaria was the main component of the wine grape mycobiota of the DOC San Rafael at harvest time (81%) followed by Cladosporium (7%) and only in minor percentage by Penicillium (4%) and Aspergillus (3%) among others. The application of an organic or non-organic treatment in the vineyard did not affect significantly the incidence of the present genera. According to morphological and molecular identification based on the genetic marker Alt a 1, all Alternaria isolates were included into Alternaria alternata species. Of 34 analyzed Alternaria strains, 97% were able to produce at least one of the three mycotoxins analyzed: alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TA) and 53% co-produced the three mycotoxins. TA was the toxin produced at highest frequency (97%) and at highest levels in a range from 11.2 to 1941.0ppm. It was followed by AOH produced by 71% of the strains, in a range from 1.8 to 437.0ppm and AME produced by 59% of the strains, in a range from 0.6ppm to 663.4ppm. The 55% of the Alternaria strains were very pathogenic, 31% moderately pathogenic and only 14% were slightly pathogenic. In the present work, a high incidence and prevalence of Alternaria genus was reported despite the use of routine control viticulture practices, as well as a high percentage of toxicogenic and pathogenic Alternaria strains.
Fil: Prendes, Luciana Paola. Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Merín, María Gabriela. Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Andreoni, Mario A.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Ramirez, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Centro de Investigaciones Cientificas ; Facultad de Ciencias Exactas Fisicas y Naturales ; Universidad Nacional de Cordoba;
Fil: Morata, Vilma Ines. Universidad Nacional de Cuyo. Facultad de Ciencias Aplicadas a la Industria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
description Alternaria, one of the most mycotoxigenic genus commonly found in wine grapes, could represent a high risk for wine consumer's health. The aims of this work were to identify the mycobiota of Malbec wine grapes under the influence of routine control viticulture practices, to identify Alternaria spp. strains by morphological and molecular methods and characterize their toxicogenic ability and pathogenicity. Alternaria was the main component of the wine grape mycobiota of the DOC San Rafael at harvest time (81%) followed by Cladosporium (7%) and only in minor percentage by Penicillium (4%) and Aspergillus (3%) among others. The application of an organic or non-organic treatment in the vineyard did not affect significantly the incidence of the present genera. According to morphological and molecular identification based on the genetic marker Alt a 1, all Alternaria isolates were included into Alternaria alternata species. Of 34 analyzed Alternaria strains, 97% were able to produce at least one of the three mycotoxins analyzed: alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TA) and 53% co-produced the three mycotoxins. TA was the toxin produced at highest frequency (97%) and at highest levels in a range from 11.2 to 1941.0ppm. It was followed by AOH produced by 71% of the strains, in a range from 1.8 to 437.0ppm and AME produced by 59% of the strains, in a range from 0.6ppm to 663.4ppm. The 55% of the Alternaria strains were very pathogenic, 31% moderately pathogenic and only 14% were slightly pathogenic. In the present work, a high incidence and prevalence of Alternaria genus was reported despite the use of routine control viticulture practices, as well as a high percentage of toxicogenic and pathogenic Alternaria strains.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180196
Prendes, Luciana Paola; Merín, María Gabriela; Andreoni, Mario A.; Ramirez, Maria Laura; Morata, Vilma Ines; Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina; Elsevier; Food Control; 57; 3-2015; 122-128
0956-7135
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180196
identifier_str_mv Prendes, Luciana Paola; Merín, María Gabriela; Andreoni, Mario A.; Ramirez, Maria Laura; Morata, Vilma Ines; Mycobiota and toxicogenic Alternaria spp. strains in Malbec wine grapes from DOC San Rafael, Mendoza, Argentina; Elsevier; Food Control; 57; 3-2015; 122-128
0956-7135
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0956713515002169
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodcont.2015.03.041
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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