Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression

Autores
Da Cruz Cabral, Lucía Mariana; Rodríguez, Alicia; Delgado, Josué; Patriarca, Andrea Rosana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BackgroundTomato fruits are susceptible to Alternaria spp. spoilage. A correct postharvest management is necessary to prevent mould growth and mycotoxin accumulation, being the temperature one of the main factors. The effect of different postharvest temperatures (5, 12, 25 and 35 °C) on growth, mycotoxin production and a stress-related gene expression by two Alternaria spp. was assessed. ResultsGrowth rates decreased rapidly when temperature was higher than the optimum (25 °C), while a gradual reduction was detected at lower temperatures. Tenuazonic acid (TeA) was strongly synthesised at all temperatures evaluated, with a maximum between 12 and 25 °C. Alternariol monomethyl ether (AME) was produced only at the two lowest temperatures; with a peak at 12 °C. Regarding the expression of the stress-related RHO1 gene, during active fungal growth both Alternaria spp. showed more copies of the gene as temperature increased. At the stationary phase, the RHO1 gene expression was significantly higher at 12 °C, coinciding with AME highest accumulation. ConclusionChanges on temperatures related to different postharvest stages of tomato fruits markedly affect toxigenic Alternaria spp. The highest levels of both mycotoxins were recorded at 12 °C, a common storage temperature for tomato fruit. Additionally, an association between alternariols biosynthesis and the cell wall integrity pathway was noticed in relation to temperature, suggesting that temperature may act as stressor stimulating the RHO1 gene expression, which in turn triggers this mycotoxin synthesis. These results will be useful in developing new strategies to efficiently control Alternaria spoilage in tomato fruit and by-products.
Fil: Da Cruz Cabral, Lucía Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Universidad de Extremadura; España
Fil: Rodríguez, Alicia. Universidad de Extremadura; España
Fil: Delgado, Josué. Universidad de Extremadura; España
Fil: Patriarca, Andrea Rosana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Materia
ALTERNARIA
ALTERNARIOL MONOMETHYL ETHER
FOOD SAFETY
TENUAZONIC ACID
TOMATO FRUIT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151256
Acceder
id CONICETDig_088e1b57fe4ba7e6a00803babcd597d4
oai_identifier_str oai:ri.conicet.gov.ar:11336/151256
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expressionDa Cruz Cabral, Lucía MarianaRodríguez, AliciaDelgado, JosuéPatriarca, Andrea RosanaALTERNARIAALTERNARIOL MONOMETHYL ETHERFOOD SAFETYTENUAZONIC ACIDTOMATO FRUIThttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1BackgroundTomato fruits are susceptible to Alternaria spp. spoilage. A correct postharvest management is necessary to prevent mould growth and mycotoxin accumulation, being the temperature one of the main factors. The effect of different postharvest temperatures (5, 12, 25 and 35 °C) on growth, mycotoxin production and a stress-related gene expression by two Alternaria spp. was assessed. ResultsGrowth rates decreased rapidly when temperature was higher than the optimum (25 °C), while a gradual reduction was detected at lower temperatures. Tenuazonic acid (TeA) was strongly synthesised at all temperatures evaluated, with a maximum between 12 and 25 °C. Alternariol monomethyl ether (AME) was produced only at the two lowest temperatures; with a peak at 12 °C. Regarding the expression of the stress-related RHO1 gene, during active fungal growth both Alternaria spp. showed more copies of the gene as temperature increased. At the stationary phase, the RHO1 gene expression was significantly higher at 12 °C, coinciding with AME highest accumulation. ConclusionChanges on temperatures related to different postharvest stages of tomato fruits markedly affect toxigenic Alternaria spp. The highest levels of both mycotoxins were recorded at 12 °C, a common storage temperature for tomato fruit. Additionally, an association between alternariols biosynthesis and the cell wall integrity pathway was noticed in relation to temperature, suggesting that temperature may act as stressor stimulating the RHO1 gene expression, which in turn triggers this mycotoxin synthesis. These results will be useful in developing new strategies to efficiently control Alternaria spoilage in tomato fruit and by-products.Fil: Da Cruz Cabral, Lucía Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Universidad de Extremadura; EspañaFil: Rodríguez, Alicia. Universidad de Extremadura; EspañaFil: Delgado, Josué. Universidad de Extremadura; EspañaFil: Patriarca, Andrea Rosana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaJohn Wiley & Sons Ltd2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151256Da Cruz Cabral, Lucía Mariana; Rodríguez, Alicia; Delgado, Josué; Patriarca, Andrea Rosana; Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression; John Wiley & Sons Ltd; Journal of the Science of Food and Agriculture; 99; 15; 12-2019; 6689-66950022-5142CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.9950info:eu-repo/semantics/altIdentifier/doi/10.1002/jsfa.9950info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:26:18Zoai:ri.conicet.gov.ar:11336/151256instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:26:18.903CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression
title Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression
spellingShingle Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression
Da Cruz Cabral, Lucía Mariana
ALTERNARIA
ALTERNARIOL MONOMETHYL ETHER
FOOD SAFETY
TENUAZONIC ACID
TOMATO FRUIT
title_short Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression
title_full Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression
title_fullStr Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression
title_full_unstemmed Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression
title_sort Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression
dc.creator.none.fl_str_mv Da Cruz Cabral, Lucía Mariana
Rodríguez, Alicia
Delgado, Josué
Patriarca, Andrea Rosana
author Da Cruz Cabral, Lucía Mariana
author_facet Da Cruz Cabral, Lucía Mariana
Rodríguez, Alicia
Delgado, Josué
Patriarca, Andrea Rosana
author_role author
author2 Rodríguez, Alicia
Delgado, Josué
Patriarca, Andrea Rosana
author2_role author
author
author
dc.subject.none.fl_str_mv ALTERNARIA
ALTERNARIOL MONOMETHYL ETHER
FOOD SAFETY
TENUAZONIC ACID
TOMATO FRUIT
topic ALTERNARIA
ALTERNARIOL MONOMETHYL ETHER
FOOD SAFETY
TENUAZONIC ACID
TOMATO FRUIT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv BackgroundTomato fruits are susceptible to Alternaria spp. spoilage. A correct postharvest management is necessary to prevent mould growth and mycotoxin accumulation, being the temperature one of the main factors. The effect of different postharvest temperatures (5, 12, 25 and 35 °C) on growth, mycotoxin production and a stress-related gene expression by two Alternaria spp. was assessed. ResultsGrowth rates decreased rapidly when temperature was higher than the optimum (25 °C), while a gradual reduction was detected at lower temperatures. Tenuazonic acid (TeA) was strongly synthesised at all temperatures evaluated, with a maximum between 12 and 25 °C. Alternariol monomethyl ether (AME) was produced only at the two lowest temperatures; with a peak at 12 °C. Regarding the expression of the stress-related RHO1 gene, during active fungal growth both Alternaria spp. showed more copies of the gene as temperature increased. At the stationary phase, the RHO1 gene expression was significantly higher at 12 °C, coinciding with AME highest accumulation. ConclusionChanges on temperatures related to different postharvest stages of tomato fruits markedly affect toxigenic Alternaria spp. The highest levels of both mycotoxins were recorded at 12 °C, a common storage temperature for tomato fruit. Additionally, an association between alternariols biosynthesis and the cell wall integrity pathway was noticed in relation to temperature, suggesting that temperature may act as stressor stimulating the RHO1 gene expression, which in turn triggers this mycotoxin synthesis. These results will be useful in developing new strategies to efficiently control Alternaria spoilage in tomato fruit and by-products.
Fil: Da Cruz Cabral, Lucía Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Universidad de Extremadura; España
Fil: Rodríguez, Alicia. Universidad de Extremadura; España
Fil: Delgado, Josué. Universidad de Extremadura; España
Fil: Patriarca, Andrea Rosana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
description BackgroundTomato fruits are susceptible to Alternaria spp. spoilage. A correct postharvest management is necessary to prevent mould growth and mycotoxin accumulation, being the temperature one of the main factors. The effect of different postharvest temperatures (5, 12, 25 and 35 °C) on growth, mycotoxin production and a stress-related gene expression by two Alternaria spp. was assessed. ResultsGrowth rates decreased rapidly when temperature was higher than the optimum (25 °C), while a gradual reduction was detected at lower temperatures. Tenuazonic acid (TeA) was strongly synthesised at all temperatures evaluated, with a maximum between 12 and 25 °C. Alternariol monomethyl ether (AME) was produced only at the two lowest temperatures; with a peak at 12 °C. Regarding the expression of the stress-related RHO1 gene, during active fungal growth both Alternaria spp. showed more copies of the gene as temperature increased. At the stationary phase, the RHO1 gene expression was significantly higher at 12 °C, coinciding with AME highest accumulation. ConclusionChanges on temperatures related to different postharvest stages of tomato fruits markedly affect toxigenic Alternaria spp. The highest levels of both mycotoxins were recorded at 12 °C, a common storage temperature for tomato fruit. Additionally, an association between alternariols biosynthesis and the cell wall integrity pathway was noticed in relation to temperature, suggesting that temperature may act as stressor stimulating the RHO1 gene expression, which in turn triggers this mycotoxin synthesis. These results will be useful in developing new strategies to efficiently control Alternaria spoilage in tomato fruit and by-products.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151256
Da Cruz Cabral, Lucía Mariana; Rodríguez, Alicia; Delgado, Josué; Patriarca, Andrea Rosana; Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression; John Wiley & Sons Ltd; Journal of the Science of Food and Agriculture; 99; 15; 12-2019; 6689-6695
0022-5142
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151256
identifier_str_mv Da Cruz Cabral, Lucía Mariana; Rodríguez, Alicia; Delgado, Josué; Patriarca, Andrea Rosana; Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell-wall integrity-related gene expression; John Wiley & Sons Ltd; Journal of the Science of Food and Agriculture; 99; 15; 12-2019; 6689-6695
0022-5142
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.9950
info:eu-repo/semantics/altIdentifier/doi/10.1002/jsfa.9950
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082707080085504
score 13.221938

Publicaciones similares