Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study
- Autores
- Rojas Panozo, Lizeth; Rivera Nina, Silvia; Wehrendt, Diana Patricia; Casellas, Aina; Pinto, Lilian; Mendez, Susana; Kuo, Chi Wei; Lozano, Daniel F.; Ortiz, Lourdes; Pinazo, Maria Jesus; Picado, Albert; Sanz, Sergi; Abril, Marcelo; Gascon, Joaquim; Wong, Season; Schijman, Alejandro Gabriel; Torrico, Faustino; Alonso Padilla, Julio
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Vertical transmission of Trypanosoma cruzi represents approximately 20% of new Chagas disease cases. Early detection and treatment for women of childbearing age and newborns is a public health priority, but the lack of a simple and reliable diagnostic test remains a major barrier. We aimed to evaluate the performance of a point-of-care loop-mediated isothermal amplification (LAMP) assay for the detection of T cruzi. In this proof-of-concept study, we coupled a low-cost 3D printer repurposed for sample preparation and amplification (PrintrLab) to the Eiken T cruzi-LAMP prototype to detect vertically transmitted T cruzi, which we compared with standardised PCR and with the gold-standard algorithm (microscopy at birth and 2 months and serological study several months later). We screened pregnant women from two hospitals in the Bolivian Gran Chaco province, and those who were seropositive for T cruzi were offered the opportunity for their newborns to be enrolled in the study. Newborns were tested by microscopy, LAMP, and PCR at birth and 2 months, and by serology at 8 months.Between April 23 and Nov 17, 2018, 986 mothers were screened, among whom 276 were seropositive for T cruzi (28·0% prevalence, 95% CI 25·6-31·2). In total, 224 infants born to 221 seropositive mothers completed 8 months of follow-up. Congenital transmission was detected in nine of the 224 newborns (4·0% prevalence, 1·9-7·5) by direct microscopy observation, and 14 more cases were diagnosed serologically (6·3%, 3·6-10·3), accounting for an overall vertical transmission rate of 10·3% (6·6-15·0; 23 of 224). All microscopy-positive newborns were positive by PrintrLab-LAMP and by PCR, while these techniques respectively detected four and five extra positive cases among the remaining 215 microscopy-negative newborns. The PrintrLab-LAMP yielded a higher sensitivity than microscopy-based analysis. Considering the simpler use and expected lower cost of LAMP compared with PCR, our findings encourage its evaluation in a larger study over a wider geographical area.
Fil: Rojas Panozo, Lizeth. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; Bolivia
Fil: Rivera Nina, Silvia. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; Bolivia
Fil: Wehrendt, Diana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Casellas, Aina. Universidad de Barcelona; España
Fil: Pinto, Lilian. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; Bolivia
Fil: Mendez, Susana. Universidad de Barcelona; España
Fil: Kuo, Chi Wei. Ai Biosciences, Inc.; Estados Unidos
Fil: Lozano, Daniel F.. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; Bolivia
Fil: Ortiz, Lourdes. Universidad Autónoma Juan Misael Saracho.; Bolivia
Fil: Pinazo, Maria Jesus. Drugs for Neglected Diseases Initiative; Brasil
Fil: Picado, Albert. Foundation For Innovative New Diagnostics; Suiza
Fil: Sanz, Sergi. Universidad de Barcelona; España
Fil: Abril, Marcelo. Fundación Mundo Sano; Argentina
Fil: Gascon, Joaquim. Universidad de Barcelona; España
Fil: Wong, Season. Ai Biosciences, Inc.; Estados Unidos
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Torrico, Faustino. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; Bolivia
Fil: Alonso Padilla, Julio. Universidad de Barcelona; España - Materia
-
CHAGAS DISEASE
VERTICAL TRANSMISSION
REPURPOSED 3D PRINTER
LAMP - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/246113
Ver los metadatos del registro completo
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Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept studyRojas Panozo, LizethRivera Nina, SilviaWehrendt, Diana PatriciaCasellas, AinaPinto, LilianMendez, SusanaKuo, Chi WeiLozano, Daniel F.Ortiz, LourdesPinazo, Maria JesusPicado, AlbertSanz, SergiAbril, MarceloGascon, JoaquimWong, SeasonSchijman, Alejandro GabrielTorrico, FaustinoAlonso Padilla, JulioCHAGAS DISEASEVERTICAL TRANSMISSIONREPURPOSED 3D PRINTERLAMPhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Vertical transmission of Trypanosoma cruzi represents approximately 20% of new Chagas disease cases. Early detection and treatment for women of childbearing age and newborns is a public health priority, but the lack of a simple and reliable diagnostic test remains a major barrier. We aimed to evaluate the performance of a point-of-care loop-mediated isothermal amplification (LAMP) assay for the detection of T cruzi. In this proof-of-concept study, we coupled a low-cost 3D printer repurposed for sample preparation and amplification (PrintrLab) to the Eiken T cruzi-LAMP prototype to detect vertically transmitted T cruzi, which we compared with standardised PCR and with the gold-standard algorithm (microscopy at birth and 2 months and serological study several months later). We screened pregnant women from two hospitals in the Bolivian Gran Chaco province, and those who were seropositive for T cruzi were offered the opportunity for their newborns to be enrolled in the study. Newborns were tested by microscopy, LAMP, and PCR at birth and 2 months, and by serology at 8 months.Between April 23 and Nov 17, 2018, 986 mothers were screened, among whom 276 were seropositive for T cruzi (28·0% prevalence, 95% CI 25·6-31·2). In total, 224 infants born to 221 seropositive mothers completed 8 months of follow-up. Congenital transmission was detected in nine of the 224 newborns (4·0% prevalence, 1·9-7·5) by direct microscopy observation, and 14 more cases were diagnosed serologically (6·3%, 3·6-10·3), accounting for an overall vertical transmission rate of 10·3% (6·6-15·0; 23 of 224). All microscopy-positive newborns were positive by PrintrLab-LAMP and by PCR, while these techniques respectively detected four and five extra positive cases among the remaining 215 microscopy-negative newborns. The PrintrLab-LAMP yielded a higher sensitivity than microscopy-based analysis. Considering the simpler use and expected lower cost of LAMP compared with PCR, our findings encourage its evaluation in a larger study over a wider geographical area.Fil: Rojas Panozo, Lizeth. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; BoliviaFil: Rivera Nina, Silvia. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; BoliviaFil: Wehrendt, Diana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Casellas, Aina. Universidad de Barcelona; EspañaFil: Pinto, Lilian. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; BoliviaFil: Mendez, Susana. Universidad de Barcelona; EspañaFil: Kuo, Chi Wei. Ai Biosciences, Inc.; Estados UnidosFil: Lozano, Daniel F.. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; BoliviaFil: Ortiz, Lourdes. Universidad Autónoma Juan Misael Saracho.; BoliviaFil: Pinazo, Maria Jesus. Drugs for Neglected Diseases Initiative; BrasilFil: Picado, Albert. Foundation For Innovative New Diagnostics; SuizaFil: Sanz, Sergi. Universidad de Barcelona; EspañaFil: Abril, Marcelo. Fundación Mundo Sano; ArgentinaFil: Gascon, Joaquim. Universidad de Barcelona; EspañaFil: Wong, Season. Ai Biosciences, Inc.; Estados UnidosFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Torrico, Faustino. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; BoliviaFil: Alonso Padilla, Julio. Universidad de Barcelona; EspañaElsevier2024-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/246113Rojas Panozo, Lizeth; Rivera Nina, Silvia; Wehrendt, Diana Patricia; Casellas, Aina; Pinto, Lilian; et al.; Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study; Elsevier; The Lancet Microbe; 5; 9; 9-2024; 1-82666-5247CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2666524724001101info:eu-repo/semantics/altIdentifier/doi/10.1016/S2666-5247(24)00110-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T09:12:59Zoai:ri.conicet.gov.ar:11336/246113instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 09:12:59.395CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study |
| title |
Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study |
| spellingShingle |
Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study Rojas Panozo, Lizeth CHAGAS DISEASE VERTICAL TRANSMISSION REPURPOSED 3D PRINTER LAMP |
| title_short |
Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study |
| title_full |
Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study |
| title_fullStr |
Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study |
| title_full_unstemmed |
Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study |
| title_sort |
Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study |
| dc.creator.none.fl_str_mv |
Rojas Panozo, Lizeth Rivera Nina, Silvia Wehrendt, Diana Patricia Casellas, Aina Pinto, Lilian Mendez, Susana Kuo, Chi Wei Lozano, Daniel F. Ortiz, Lourdes Pinazo, Maria Jesus Picado, Albert Sanz, Sergi Abril, Marcelo Gascon, Joaquim Wong, Season Schijman, Alejandro Gabriel Torrico, Faustino Alonso Padilla, Julio |
| author |
Rojas Panozo, Lizeth |
| author_facet |
Rojas Panozo, Lizeth Rivera Nina, Silvia Wehrendt, Diana Patricia Casellas, Aina Pinto, Lilian Mendez, Susana Kuo, Chi Wei Lozano, Daniel F. Ortiz, Lourdes Pinazo, Maria Jesus Picado, Albert Sanz, Sergi Abril, Marcelo Gascon, Joaquim Wong, Season Schijman, Alejandro Gabriel Torrico, Faustino Alonso Padilla, Julio |
| author_role |
author |
| author2 |
Rivera Nina, Silvia Wehrendt, Diana Patricia Casellas, Aina Pinto, Lilian Mendez, Susana Kuo, Chi Wei Lozano, Daniel F. Ortiz, Lourdes Pinazo, Maria Jesus Picado, Albert Sanz, Sergi Abril, Marcelo Gascon, Joaquim Wong, Season Schijman, Alejandro Gabriel Torrico, Faustino Alonso Padilla, Julio |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
CHAGAS DISEASE VERTICAL TRANSMISSION REPURPOSED 3D PRINTER LAMP |
| topic |
CHAGAS DISEASE VERTICAL TRANSMISSION REPURPOSED 3D PRINTER LAMP |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
Vertical transmission of Trypanosoma cruzi represents approximately 20% of new Chagas disease cases. Early detection and treatment for women of childbearing age and newborns is a public health priority, but the lack of a simple and reliable diagnostic test remains a major barrier. We aimed to evaluate the performance of a point-of-care loop-mediated isothermal amplification (LAMP) assay for the detection of T cruzi. In this proof-of-concept study, we coupled a low-cost 3D printer repurposed for sample preparation and amplification (PrintrLab) to the Eiken T cruzi-LAMP prototype to detect vertically transmitted T cruzi, which we compared with standardised PCR and with the gold-standard algorithm (microscopy at birth and 2 months and serological study several months later). We screened pregnant women from two hospitals in the Bolivian Gran Chaco province, and those who were seropositive for T cruzi were offered the opportunity for their newborns to be enrolled in the study. Newborns were tested by microscopy, LAMP, and PCR at birth and 2 months, and by serology at 8 months.Between April 23 and Nov 17, 2018, 986 mothers were screened, among whom 276 were seropositive for T cruzi (28·0% prevalence, 95% CI 25·6-31·2). In total, 224 infants born to 221 seropositive mothers completed 8 months of follow-up. Congenital transmission was detected in nine of the 224 newborns (4·0% prevalence, 1·9-7·5) by direct microscopy observation, and 14 more cases were diagnosed serologically (6·3%, 3·6-10·3), accounting for an overall vertical transmission rate of 10·3% (6·6-15·0; 23 of 224). All microscopy-positive newborns were positive by PrintrLab-LAMP and by PCR, while these techniques respectively detected four and five extra positive cases among the remaining 215 microscopy-negative newborns. The PrintrLab-LAMP yielded a higher sensitivity than microscopy-based analysis. Considering the simpler use and expected lower cost of LAMP compared with PCR, our findings encourage its evaluation in a larger study over a wider geographical area. Fil: Rojas Panozo, Lizeth. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; Bolivia Fil: Rivera Nina, Silvia. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; Bolivia Fil: Wehrendt, Diana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Casellas, Aina. Universidad de Barcelona; España Fil: Pinto, Lilian. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; Bolivia Fil: Mendez, Susana. Universidad de Barcelona; España Fil: Kuo, Chi Wei. Ai Biosciences, Inc.; Estados Unidos Fil: Lozano, Daniel F.. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; Bolivia Fil: Ortiz, Lourdes. Universidad Autónoma Juan Misael Saracho.; Bolivia Fil: Pinazo, Maria Jesus. Drugs for Neglected Diseases Initiative; Brasil Fil: Picado, Albert. Foundation For Innovative New Diagnostics; Suiza Fil: Sanz, Sergi. Universidad de Barcelona; España Fil: Abril, Marcelo. Fundación Mundo Sano; Argentina Fil: Gascon, Joaquim. Universidad de Barcelona; España Fil: Wong, Season. Ai Biosciences, Inc.; Estados Unidos Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Torrico, Faustino. Fundación Ciencia y Estudios Aplicados para el Desarrollo en Salud y Medio Ambiente ; Bolivia Fil: Alonso Padilla, Julio. Universidad de Barcelona; España |
| description |
Vertical transmission of Trypanosoma cruzi represents approximately 20% of new Chagas disease cases. Early detection and treatment for women of childbearing age and newborns is a public health priority, but the lack of a simple and reliable diagnostic test remains a major barrier. We aimed to evaluate the performance of a point-of-care loop-mediated isothermal amplification (LAMP) assay for the detection of T cruzi. In this proof-of-concept study, we coupled a low-cost 3D printer repurposed for sample preparation and amplification (PrintrLab) to the Eiken T cruzi-LAMP prototype to detect vertically transmitted T cruzi, which we compared with standardised PCR and with the gold-standard algorithm (microscopy at birth and 2 months and serological study several months later). We screened pregnant women from two hospitals in the Bolivian Gran Chaco province, and those who were seropositive for T cruzi were offered the opportunity for their newborns to be enrolled in the study. Newborns were tested by microscopy, LAMP, and PCR at birth and 2 months, and by serology at 8 months.Between April 23 and Nov 17, 2018, 986 mothers were screened, among whom 276 were seropositive for T cruzi (28·0% prevalence, 95% CI 25·6-31·2). In total, 224 infants born to 221 seropositive mothers completed 8 months of follow-up. Congenital transmission was detected in nine of the 224 newborns (4·0% prevalence, 1·9-7·5) by direct microscopy observation, and 14 more cases were diagnosed serologically (6·3%, 3·6-10·3), accounting for an overall vertical transmission rate of 10·3% (6·6-15·0; 23 of 224). All microscopy-positive newborns were positive by PrintrLab-LAMP and by PCR, while these techniques respectively detected four and five extra positive cases among the remaining 215 microscopy-negative newborns. The PrintrLab-LAMP yielded a higher sensitivity than microscopy-based analysis. Considering the simpler use and expected lower cost of LAMP compared with PCR, our findings encourage its evaluation in a larger study over a wider geographical area. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-09 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/246113 Rojas Panozo, Lizeth; Rivera Nina, Silvia; Wehrendt, Diana Patricia; Casellas, Aina; Pinto, Lilian; et al.; Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study; Elsevier; The Lancet Microbe; 5; 9; 9-2024; 1-8 2666-5247 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/246113 |
| identifier_str_mv |
Rojas Panozo, Lizeth; Rivera Nina, Silvia; Wehrendt, Diana Patricia; Casellas, Aina; Pinto, Lilian; et al.; Evaluation and validation of a PrintrLab-based LAMP assay to identify Trypanosoma cruzi in newborns in Bolivia: A proof-of-concept study; Elsevier; The Lancet Microbe; 5; 9; 9-2024; 1-8 2666-5247 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2666524724001101 info:eu-repo/semantics/altIdentifier/doi/10.1016/S2666-5247(24)00110-1 |
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