Rabies virus nucleoprotein: Large-scale expression in prokaryotic system

Autores
Picotto, Leandro Daniel; Panei, Carlos Javier; Pecoraro, Marcelo Ricardo; Sguazza, Guillermo Hernán
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rabies is controlled by mass animal vaccination campaigns. Cats, dogs, and wild animals (e.g., bats) are large reservoirs of this virus and can pose a significant threat to the human health, especially in the developing countries. The nucleoprotein of the rabies virus is of great scientific interest since it has the potential to generate immunity in animals and can be used as for immunochemical diagnostics. The study aimed to test a large-scale expression of the rabies N protein in a prokaryotic system. The recombinant N protein was successfully expressed and purified. It was immunologically recognized by specific antibodies and was able to induce the production of specific antibodies in a mouse immunization assay. These encouraging results indicate that the recombinant N protein can be evaluated as an antigen for the development of a subunit vaccine or for a diagnostic assay.
Fil: Picotto, Leandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Panei, Carlos Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Pecoraro, Marcelo Ricardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Sguazza, Guillermo Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
Materia
Rabies virus
Nucleoprotein
Escherichia coli
Large-scale expression
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244897
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Rabies virus nucleoprotein: Large-scale expression in prokaryotic systemPicotto, Leandro DanielPanei, Carlos JavierPecoraro, Marcelo RicardoSguazza, Guillermo HernánRabies virusNucleoproteinEscherichia coliLarge-scale expressionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rabies is controlled by mass animal vaccination campaigns. Cats, dogs, and wild animals (e.g., bats) are large reservoirs of this virus and can pose a significant threat to the human health, especially in the developing countries. The nucleoprotein of the rabies virus is of great scientific interest since it has the potential to generate immunity in animals and can be used as for immunochemical diagnostics. The study aimed to test a large-scale expression of the rabies N protein in a prokaryotic system. The recombinant N protein was successfully expressed and purified. It was immunologically recognized by specific antibodies and was able to induce the production of specific antibodies in a mouse immunization assay. These encouraging results indicate that the recombinant N protein can be evaluated as an antigen for the development of a subunit vaccine or for a diagnostic assay.Fil: Picotto, Leandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Panei, Carlos Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Pecoraro, Marcelo Ricardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Sguazza, Guillermo Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaMethodius University. Faculty of Veterinary Medicin2024-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244897Picotto, Leandro Daniel; Panei, Carlos Javier; Pecoraro, Marcelo Ricardo; Sguazza, Guillermo Hernán; Rabies virus nucleoprotein: Large-scale expression in prokaryotic system; Methodius University. Faculty of Veterinary Medicin; Macedonian Veterinary Review; 47; 2; 9-2024; 1-81409-76211857-7415CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sciendo.com/es/article/10.2478/macvetrev-2024-0026info:eu-repo/semantics/altIdentifier/doi/10.2478/macvetrev-2024-0026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:09Zoai:ri.conicet.gov.ar:11336/244897instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:09.401CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rabies virus nucleoprotein: Large-scale expression in prokaryotic system
title Rabies virus nucleoprotein: Large-scale expression in prokaryotic system
spellingShingle Rabies virus nucleoprotein: Large-scale expression in prokaryotic system
Picotto, Leandro Daniel
Rabies virus
Nucleoprotein
Escherichia coli
Large-scale expression
title_short Rabies virus nucleoprotein: Large-scale expression in prokaryotic system
title_full Rabies virus nucleoprotein: Large-scale expression in prokaryotic system
title_fullStr Rabies virus nucleoprotein: Large-scale expression in prokaryotic system
title_full_unstemmed Rabies virus nucleoprotein: Large-scale expression in prokaryotic system
title_sort Rabies virus nucleoprotein: Large-scale expression in prokaryotic system
dc.creator.none.fl_str_mv Picotto, Leandro Daniel
Panei, Carlos Javier
Pecoraro, Marcelo Ricardo
Sguazza, Guillermo Hernán
author Picotto, Leandro Daniel
author_facet Picotto, Leandro Daniel
Panei, Carlos Javier
Pecoraro, Marcelo Ricardo
Sguazza, Guillermo Hernán
author_role author
author2 Panei, Carlos Javier
Pecoraro, Marcelo Ricardo
Sguazza, Guillermo Hernán
author2_role author
author
author
dc.subject.none.fl_str_mv Rabies virus
Nucleoprotein
Escherichia coli
Large-scale expression
topic Rabies virus
Nucleoprotein
Escherichia coli
Large-scale expression
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rabies is controlled by mass animal vaccination campaigns. Cats, dogs, and wild animals (e.g., bats) are large reservoirs of this virus and can pose a significant threat to the human health, especially in the developing countries. The nucleoprotein of the rabies virus is of great scientific interest since it has the potential to generate immunity in animals and can be used as for immunochemical diagnostics. The study aimed to test a large-scale expression of the rabies N protein in a prokaryotic system. The recombinant N protein was successfully expressed and purified. It was immunologically recognized by specific antibodies and was able to induce the production of specific antibodies in a mouse immunization assay. These encouraging results indicate that the recombinant N protein can be evaluated as an antigen for the development of a subunit vaccine or for a diagnostic assay.
Fil: Picotto, Leandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Panei, Carlos Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Pecoraro, Marcelo Ricardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Sguazza, Guillermo Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina
description Rabies is controlled by mass animal vaccination campaigns. Cats, dogs, and wild animals (e.g., bats) are large reservoirs of this virus and can pose a significant threat to the human health, especially in the developing countries. The nucleoprotein of the rabies virus is of great scientific interest since it has the potential to generate immunity in animals and can be used as for immunochemical diagnostics. The study aimed to test a large-scale expression of the rabies N protein in a prokaryotic system. The recombinant N protein was successfully expressed and purified. It was immunologically recognized by specific antibodies and was able to induce the production of specific antibodies in a mouse immunization assay. These encouraging results indicate that the recombinant N protein can be evaluated as an antigen for the development of a subunit vaccine or for a diagnostic assay.
publishDate 2024
dc.date.none.fl_str_mv 2024-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244897
Picotto, Leandro Daniel; Panei, Carlos Javier; Pecoraro, Marcelo Ricardo; Sguazza, Guillermo Hernán; Rabies virus nucleoprotein: Large-scale expression in prokaryotic system; Methodius University. Faculty of Veterinary Medicin; Macedonian Veterinary Review; 47; 2; 9-2024; 1-8
1409-7621
1857-7415
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244897
identifier_str_mv Picotto, Leandro Daniel; Panei, Carlos Javier; Pecoraro, Marcelo Ricardo; Sguazza, Guillermo Hernán; Rabies virus nucleoprotein: Large-scale expression in prokaryotic system; Methodius University. Faculty of Veterinary Medicin; Macedonian Veterinary Review; 47; 2; 9-2024; 1-8
1409-7621
1857-7415
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sciendo.com/es/article/10.2478/macvetrev-2024-0026
info:eu-repo/semantics/altIdentifier/doi/10.2478/macvetrev-2024-0026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Methodius University. Faculty of Veterinary Medicin
publisher.none.fl_str_mv Methodius University. Faculty of Veterinary Medicin
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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