Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system

Autores
Zhang, Hongtao; Palma, Angelina S.; Zhang, Yibing; Childs, Robert A.; Liu, Yan; Mitchell, Daniel A.; Guidolin, Leticia Soledad; Weigel, Wilfried; Mulloy, Barbara; Ciocchini, Andres Eduardo; Feizi, Ten; Chai, Wengang
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The β1,2-glucans produced by bacteria are important in invasion, survival and immunomodulation in infected hosts be they mammals or plants. However, there has been a lack of information on proteins which recognize these molecules. This is partly due to the extremely limited availability of the sequence-defined oligosaccharides and derived probes for use in the study of their interactions. Here we have used the cyclic β1,2-glucan (CβG) of the bacterial pathogen Brucella abortus, after removal of succinyl side chains, to prepare linearized oligosaccharides which were used to generate microarrays. We describe optimized conditions for partial depolymerization of the cyclic glucan by acid hydrolysis and conversion of the β1,2-gluco-oligosaccharides, with degrees of polymerization 2-13, to neoglycolipids for the purpose of generating microarrays. By microarray analyses, we show that the C-type lectin receptor DC-SIGNR, like the closely related DC-SIGN we investigated earlier, binds to the β1,2-gluco-oligosaccharides, as does the soluble immune effector serum mannose-binding protein. Exploratory studies with DC-SIGN are suggestive of the recognition also of the intact CβG by this receptor. These findings open the way to unravelling mechanisms of immunomodulation mediated by β1,2-glucans in mammalian systems.
Fil: Zhang, Hongtao. Imperial College London; Reino Unido. Jiangnan University; China
Fil: Palma, Angelina S.. Imperial College London; Reino Unido. Universidade de Lisboa; Portugal
Fil: Zhang, Yibing. Imperial College London; Reino Unido
Fil: Childs, Robert A.. Imperial College London; Reino Unido
Fil: Liu, Yan. Imperial College London; Reino Unido
Fil: Mitchell, Daniel A.. University of Warwick; Reino Unido
Fil: Guidolin, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Weigel, Wilfried. SCIENION AG; Alemania
Fil: Mulloy, Barbara. Imperial College London; Reino Unido
Fil: Ciocchini, Andres Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Feizi, Ten. Imperial College London; Reino Unido
Fil: Chai, Wengang. Imperial College London; Reino Unido
Materia
C-Type Lectins
Carbohydrate Microarray
Glucan Recognition
Neoglycolipids
Β1 2-Glucan
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53424
Acceder
id CONICETDig_f6d13c8e892047721082048de7a3979d
oai_identifier_str oai:ri.conicet.gov.ar:11336/53424
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune systemZhang, HongtaoPalma, Angelina S.Zhang, YibingChilds, Robert A.Liu, YanMitchell, Daniel A.Guidolin, Leticia SoledadWeigel, WilfriedMulloy, BarbaraCiocchini, Andres EduardoFeizi, TenChai, WengangC-Type LectinsCarbohydrate MicroarrayGlucan RecognitionNeoglycolipidsΒ1 2-Glucanhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The β1,2-glucans produced by bacteria are important in invasion, survival and immunomodulation in infected hosts be they mammals or plants. However, there has been a lack of information on proteins which recognize these molecules. This is partly due to the extremely limited availability of the sequence-defined oligosaccharides and derived probes for use in the study of their interactions. Here we have used the cyclic β1,2-glucan (CβG) of the bacterial pathogen Brucella abortus, after removal of succinyl side chains, to prepare linearized oligosaccharides which were used to generate microarrays. We describe optimized conditions for partial depolymerization of the cyclic glucan by acid hydrolysis and conversion of the β1,2-gluco-oligosaccharides, with degrees of polymerization 2-13, to neoglycolipids for the purpose of generating microarrays. By microarray analyses, we show that the C-type lectin receptor DC-SIGNR, like the closely related DC-SIGN we investigated earlier, binds to the β1,2-gluco-oligosaccharides, as does the soluble immune effector serum mannose-binding protein. Exploratory studies with DC-SIGN are suggestive of the recognition also of the intact CβG by this receptor. These findings open the way to unravelling mechanisms of immunomodulation mediated by β1,2-glucans in mammalian systems.Fil: Zhang, Hongtao. Imperial College London; Reino Unido. Jiangnan University; ChinaFil: Palma, Angelina S.. Imperial College London; Reino Unido. Universidade de Lisboa; PortugalFil: Zhang, Yibing. Imperial College London; Reino UnidoFil: Childs, Robert A.. Imperial College London; Reino UnidoFil: Liu, Yan. Imperial College London; Reino UnidoFil: Mitchell, Daniel A.. University of Warwick; Reino UnidoFil: Guidolin, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Weigel, Wilfried. SCIENION AG; AlemaniaFil: Mulloy, Barbara. Imperial College London; Reino UnidoFil: Ciocchini, Andres Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Feizi, Ten. Imperial College London; Reino UnidoFil: Chai, Wengang. Imperial College London; Reino UnidoOxford Univ Press Inc2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53424Zhang, Hongtao; Palma, Angelina S.; Zhang, Yibing; Childs, Robert A.; Liu, Yan; et al.; Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system; Oxford Univ Press Inc; Glycobiology; 26; 10; 10-2016; 1086-10960959-6658CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/glycob/cww041info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/glycob/article/26/10/1086/2445953info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:28Zoai:ri.conicet.gov.ar:11336/53424instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:28.492CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system
title Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system
spellingShingle Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system
Zhang, Hongtao
C-Type Lectins
Carbohydrate Microarray
Glucan Recognition
Neoglycolipids
Β1 2-Glucan
title_short Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system
title_full Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system
title_fullStr Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system
title_full_unstemmed Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system
title_sort Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system
dc.creator.none.fl_str_mv Zhang, Hongtao
Palma, Angelina S.
Zhang, Yibing
Childs, Robert A.
Liu, Yan
Mitchell, Daniel A.
Guidolin, Leticia Soledad
Weigel, Wilfried
Mulloy, Barbara
Ciocchini, Andres Eduardo
Feizi, Ten
Chai, Wengang
author Zhang, Hongtao
author_facet Zhang, Hongtao
Palma, Angelina S.
Zhang, Yibing
Childs, Robert A.
Liu, Yan
Mitchell, Daniel A.
Guidolin, Leticia Soledad
Weigel, Wilfried
Mulloy, Barbara
Ciocchini, Andres Eduardo
Feizi, Ten
Chai, Wengang
author_role author
author2 Palma, Angelina S.
Zhang, Yibing
Childs, Robert A.
Liu, Yan
Mitchell, Daniel A.
Guidolin, Leticia Soledad
Weigel, Wilfried
Mulloy, Barbara
Ciocchini, Andres Eduardo
Feizi, Ten
Chai, Wengang
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv C-Type Lectins
Carbohydrate Microarray
Glucan Recognition
Neoglycolipids
Β1 2-Glucan
topic C-Type Lectins
Carbohydrate Microarray
Glucan Recognition
Neoglycolipids
Β1 2-Glucan
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The β1,2-glucans produced by bacteria are important in invasion, survival and immunomodulation in infected hosts be they mammals or plants. However, there has been a lack of information on proteins which recognize these molecules. This is partly due to the extremely limited availability of the sequence-defined oligosaccharides and derived probes for use in the study of their interactions. Here we have used the cyclic β1,2-glucan (CβG) of the bacterial pathogen Brucella abortus, after removal of succinyl side chains, to prepare linearized oligosaccharides which were used to generate microarrays. We describe optimized conditions for partial depolymerization of the cyclic glucan by acid hydrolysis and conversion of the β1,2-gluco-oligosaccharides, with degrees of polymerization 2-13, to neoglycolipids for the purpose of generating microarrays. By microarray analyses, we show that the C-type lectin receptor DC-SIGNR, like the closely related DC-SIGN we investigated earlier, binds to the β1,2-gluco-oligosaccharides, as does the soluble immune effector serum mannose-binding protein. Exploratory studies with DC-SIGN are suggestive of the recognition also of the intact CβG by this receptor. These findings open the way to unravelling mechanisms of immunomodulation mediated by β1,2-glucans in mammalian systems.
Fil: Zhang, Hongtao. Imperial College London; Reino Unido. Jiangnan University; China
Fil: Palma, Angelina S.. Imperial College London; Reino Unido. Universidade de Lisboa; Portugal
Fil: Zhang, Yibing. Imperial College London; Reino Unido
Fil: Childs, Robert A.. Imperial College London; Reino Unido
Fil: Liu, Yan. Imperial College London; Reino Unido
Fil: Mitchell, Daniel A.. University of Warwick; Reino Unido
Fil: Guidolin, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Weigel, Wilfried. SCIENION AG; Alemania
Fil: Mulloy, Barbara. Imperial College London; Reino Unido
Fil: Ciocchini, Andres Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Feizi, Ten. Imperial College London; Reino Unido
Fil: Chai, Wengang. Imperial College London; Reino Unido
description The β1,2-glucans produced by bacteria are important in invasion, survival and immunomodulation in infected hosts be they mammals or plants. However, there has been a lack of information on proteins which recognize these molecules. This is partly due to the extremely limited availability of the sequence-defined oligosaccharides and derived probes for use in the study of their interactions. Here we have used the cyclic β1,2-glucan (CβG) of the bacterial pathogen Brucella abortus, after removal of succinyl side chains, to prepare linearized oligosaccharides which were used to generate microarrays. We describe optimized conditions for partial depolymerization of the cyclic glucan by acid hydrolysis and conversion of the β1,2-gluco-oligosaccharides, with degrees of polymerization 2-13, to neoglycolipids for the purpose of generating microarrays. By microarray analyses, we show that the C-type lectin receptor DC-SIGNR, like the closely related DC-SIGN we investigated earlier, binds to the β1,2-gluco-oligosaccharides, as does the soluble immune effector serum mannose-binding protein. Exploratory studies with DC-SIGN are suggestive of the recognition also of the intact CβG by this receptor. These findings open the way to unravelling mechanisms of immunomodulation mediated by β1,2-glucans in mammalian systems.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53424
Zhang, Hongtao; Palma, Angelina S.; Zhang, Yibing; Childs, Robert A.; Liu, Yan; et al.; Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system; Oxford Univ Press Inc; Glycobiology; 26; 10; 10-2016; 1086-1096
0959-6658
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53424
identifier_str_mv Zhang, Hongtao; Palma, Angelina S.; Zhang, Yibing; Childs, Robert A.; Liu, Yan; et al.; Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system; Oxford Univ Press Inc; Glycobiology; 26; 10; 10-2016; 1086-1096
0959-6658
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/glycob/cww041
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/glycob/article/26/10/1086/2445953
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford Univ Press Inc
publisher.none.fl_str_mv Oxford Univ Press Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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