A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan

Autores
Werning, María Laura; Pérez Ramos, Adrián; Fernández de Palencia, Pilar; Mohedano, Mari Luz; Dueñas, María Teresa; Prieto, Alicia; López, Paloma
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Exopolysaccharides synthesized by lactic acid bacteria have prebiotic properties and contribute to the rheology and texture of fermented foods. Here, we have standardized an immunological method for the specific detection of 2-substituted (1,3)-β-d-glucans. The method allows direct detection and quantification of this exopolysaccharide in culture supernatants containing other mono- and poly-saccharides. Moreover, it allows specific detection of the biomolecules synthesized in vitro in enzymatic reactions. Thus, this method allows the fast identification of producing bacteria, as well as biochemical characterization of the glycosyltransferases responsible for their synthesis.
Fil: Werning, María Laura. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez Ramos, Adrián. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Fernández de Palencia, Pilar. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Mohedano, Mari Luz. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Dueñas, María Teresa. Universidad Complutense de Madrid. Facultad de Cs.químicas; España
Fil: Prieto, Alicia. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: López, Paloma. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Materia
Exopolysaccharide
Lactic Acid Bacteria
B-D-Glucan
Probiotic
Prebiotic
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/35695
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/35695
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucanWerning, María LauraPérez Ramos, AdriánFernández de Palencia, PilarMohedano, Mari LuzDueñas, María TeresaPrieto, AliciaLópez, PalomaExopolysaccharideLactic Acid BacteriaB-D-GlucanProbioticPrebiotichttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Exopolysaccharides synthesized by lactic acid bacteria have prebiotic properties and contribute to the rheology and texture of fermented foods. Here, we have standardized an immunological method for the specific detection of 2-substituted (1,3)-β-d-glucans. The method allows direct detection and quantification of this exopolysaccharide in culture supernatants containing other mono- and poly-saccharides. Moreover, it allows specific detection of the biomolecules synthesized in vitro in enzymatic reactions. Thus, this method allows the fast identification of producing bacteria, as well as biochemical characterization of the glycosyltransferases responsible for their synthesis.Fil: Werning, María Laura. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez Ramos, Adrián. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaFil: Fernández de Palencia, Pilar. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaFil: Mohedano, Mari Luz. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaFil: Dueñas, María Teresa. Universidad Complutense de Madrid. Facultad de Cs.químicas; EspañaFil: Prieto, Alicia. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaFil: López, Paloma. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaElsevier2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35695Werning, María Laura; Pérez Ramos, Adrián; Fernández de Palencia, Pilar; Mohedano, Mari Luz; Dueñas, María Teresa; et al.; A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan; Elsevier; Carbohydrate Polymers; 113; 7-2014; 39-450144-8617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2014.06.072info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0144861714006481info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:38Zoai:ri.conicet.gov.ar:11336/35695instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:39.023CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan
title A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan
spellingShingle A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan
Werning, María Laura
Exopolysaccharide
Lactic Acid Bacteria
B-D-Glucan
Probiotic
Prebiotic
title_short A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan
title_full A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan
title_fullStr A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan
title_full_unstemmed A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan
title_sort A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan
dc.creator.none.fl_str_mv Werning, María Laura
Pérez Ramos, Adrián
Fernández de Palencia, Pilar
Mohedano, Mari Luz
Dueñas, María Teresa
Prieto, Alicia
López, Paloma
author Werning, María Laura
author_facet Werning, María Laura
Pérez Ramos, Adrián
Fernández de Palencia, Pilar
Mohedano, Mari Luz
Dueñas, María Teresa
Prieto, Alicia
López, Paloma
author_role author
author2 Pérez Ramos, Adrián
Fernández de Palencia, Pilar
Mohedano, Mari Luz
Dueñas, María Teresa
Prieto, Alicia
López, Paloma
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Exopolysaccharide
Lactic Acid Bacteria
B-D-Glucan
Probiotic
Prebiotic
topic Exopolysaccharide
Lactic Acid Bacteria
B-D-Glucan
Probiotic
Prebiotic
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Exopolysaccharides synthesized by lactic acid bacteria have prebiotic properties and contribute to the rheology and texture of fermented foods. Here, we have standardized an immunological method for the specific detection of 2-substituted (1,3)-β-d-glucans. The method allows direct detection and quantification of this exopolysaccharide in culture supernatants containing other mono- and poly-saccharides. Moreover, it allows specific detection of the biomolecules synthesized in vitro in enzymatic reactions. Thus, this method allows the fast identification of producing bacteria, as well as biochemical characterization of the glycosyltransferases responsible for their synthesis.
Fil: Werning, María Laura. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez Ramos, Adrián. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Fernández de Palencia, Pilar. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Mohedano, Mari Luz. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Dueñas, María Teresa. Universidad Complutense de Madrid. Facultad de Cs.químicas; España
Fil: Prieto, Alicia. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: López, Paloma. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
description Exopolysaccharides synthesized by lactic acid bacteria have prebiotic properties and contribute to the rheology and texture of fermented foods. Here, we have standardized an immunological method for the specific detection of 2-substituted (1,3)-β-d-glucans. The method allows direct detection and quantification of this exopolysaccharide in culture supernatants containing other mono- and poly-saccharides. Moreover, it allows specific detection of the biomolecules synthesized in vitro in enzymatic reactions. Thus, this method allows the fast identification of producing bacteria, as well as biochemical characterization of the glycosyltransferases responsible for their synthesis.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/35695
Werning, María Laura; Pérez Ramos, Adrián; Fernández de Palencia, Pilar; Mohedano, Mari Luz; Dueñas, María Teresa; et al.; A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan; Elsevier; Carbohydrate Polymers; 113; 7-2014; 39-45
0144-8617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/35695
identifier_str_mv Werning, María Laura; Pérez Ramos, Adrián; Fernández de Palencia, Pilar; Mohedano, Mari Luz; Dueñas, María Teresa; et al.; A specific immunological method to detect and quantify bacterial 2-substituted (1,3)-β-d-glucan; Elsevier; Carbohydrate Polymers; 113; 7-2014; 39-45
0144-8617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2014.06.072
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0144861714006481
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614520703549440
score 13.070432

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