Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis

Autores
Garello, Francesca; Arena, Francesca; Cutrin, Juan Carlos; Esposito, Giovanna; D'angeli, Luca; Cesano, Federico; Filippi, Miriam; Figueiredo, Sara; Terreno, Enzo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glucan Particles (GPs) are hollow pseudo-microspheres (average diameter 3?5 mm) obtained from common baker´s yeast Saccharomyces cerevisiae, in which mannan, lipids and proteins are removed through a process of chemical extraction. GPs shell is mostly made of 1,3-b-D-glucan that allows for their prompt in vivo uptake by immune system cells. In this work, the inner cavity of the particles has been loaded with two amphiphilic fluorescent dyes (based on cyanine for in vivo imaging purposes and rhodamine for ex vivo microscopy experiments) through a sudden change in solvent polarity that allowed the entrapment of the molecules as microemulsion. The ability of fluorescent GPs to label immune cells in vivo and report on their recruitment in inflamed sites has been successfully demonstrated in a mouse model of rheumatoid arthritis (CIA) by NIRF imaging. Besides providing the visualization of the mononuclear cell infiltration in the lesion, the fluorescent signal well correlated with the clinical score associated with the disease. Very interestingly, the signal detected in lesions with the same clinical score allowed the assessment of the time evolution (progression or remission) of the pathology.
Fil: Garello, Francesca. Universitã â  Di Torino; Italia
Fil: Arena, Francesca. Universitã â  Di Torino; Italia
Fil: Cutrin, Juan Carlos. Universitã â  Di Torino; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina
Fil: Esposito, Giovanna. Universita di Torino; Italia
Fil: D'angeli, Luca. University Of London; Reino Unido
Fil: Cesano, Federico. Universita di Torino; Italia
Fil: Filippi, Miriam. Universitã â  Di Torino; Italia
Fil: Figueiredo, Sara. Universitã â  Di Torino; Italia. Universidad de Coimbra; Portugal
Fil: Terreno, Enzo. Universita di Torino; Italia
Materia
Glucan Nanoparticles
Molecular Imaging
Inflammation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7913
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritisGarello, FrancescaArena, FrancescaCutrin, Juan CarlosEsposito, GiovannaD'angeli, LucaCesano, FedericoFilippi, MiriamFigueiredo, SaraTerreno, EnzoGlucan NanoparticlesMolecular ImagingInflammationhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Glucan Particles (GPs) are hollow pseudo-microspheres (average diameter 3?5 mm) obtained from common baker´s yeast Saccharomyces cerevisiae, in which mannan, lipids and proteins are removed through a process of chemical extraction. GPs shell is mostly made of 1,3-b-D-glucan that allows for their prompt in vivo uptake by immune system cells. In this work, the inner cavity of the particles has been loaded with two amphiphilic fluorescent dyes (based on cyanine for in vivo imaging purposes and rhodamine for ex vivo microscopy experiments) through a sudden change in solvent polarity that allowed the entrapment of the molecules as microemulsion. The ability of fluorescent GPs to label immune cells in vivo and report on their recruitment in inflamed sites has been successfully demonstrated in a mouse model of rheumatoid arthritis (CIA) by NIRF imaging. Besides providing the visualization of the mononuclear cell infiltration in the lesion, the fluorescent signal well correlated with the clinical score associated with the disease. Very interestingly, the signal detected in lesions with the same clinical score allowed the assessment of the time evolution (progression or remission) of the pathology.Fil: Garello, Francesca. Universitã â  Di Torino; ItaliaFil: Arena, Francesca. Universitã â  Di Torino; ItaliaFil: Cutrin, Juan Carlos. Universitã â  Di Torino; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); ArgentinaFil: Esposito, Giovanna. Universita di Torino; ItaliaFil: D'angeli, Luca. University Of London; Reino UnidoFil: Cesano, Federico. Universita di Torino; ItaliaFil: Filippi, Miriam. Universitã â  Di Torino; ItaliaFil: Figueiredo, Sara. Universitã â  Di Torino; Italia. Universidad de Coimbra; PortugalFil: Terreno, Enzo. Universita di Torino; ItaliaRoyal Society of Chemistry2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7913Garello, Francesca; Arena, Francesca; Cutrin, Juan Carlos; Esposito, Giovanna; D'angeli, Luca; et al.; Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis; Royal Society of Chemistry; RSC Advances; 5; 43; 5-2015; 34078-340872046-2069enginfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2015/ra/c5ra00720h#!divAbstractinfo:eu-repo/semantics/altIdentifier/doi/10.1039/C5RA00720Hinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:08Zoai:ri.conicet.gov.ar:11336/7913instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:08.491CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis
title Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis
spellingShingle Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis
Garello, Francesca
Glucan Nanoparticles
Molecular Imaging
Inflammation
title_short Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis
title_full Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis
title_fullStr Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis
title_full_unstemmed Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis
title_sort Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis
dc.creator.none.fl_str_mv Garello, Francesca
Arena, Francesca
Cutrin, Juan Carlos
Esposito, Giovanna
D'angeli, Luca
Cesano, Federico
Filippi, Miriam
Figueiredo, Sara
Terreno, Enzo
author Garello, Francesca
author_facet Garello, Francesca
Arena, Francesca
Cutrin, Juan Carlos
Esposito, Giovanna
D'angeli, Luca
Cesano, Federico
Filippi, Miriam
Figueiredo, Sara
Terreno, Enzo
author_role author
author2 Arena, Francesca
Cutrin, Juan Carlos
Esposito, Giovanna
D'angeli, Luca
Cesano, Federico
Filippi, Miriam
Figueiredo, Sara
Terreno, Enzo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Glucan Nanoparticles
Molecular Imaging
Inflammation
topic Glucan Nanoparticles
Molecular Imaging
Inflammation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Glucan Particles (GPs) are hollow pseudo-microspheres (average diameter 3?5 mm) obtained from common baker´s yeast Saccharomyces cerevisiae, in which mannan, lipids and proteins are removed through a process of chemical extraction. GPs shell is mostly made of 1,3-b-D-glucan that allows for their prompt in vivo uptake by immune system cells. In this work, the inner cavity of the particles has been loaded with two amphiphilic fluorescent dyes (based on cyanine for in vivo imaging purposes and rhodamine for ex vivo microscopy experiments) through a sudden change in solvent polarity that allowed the entrapment of the molecules as microemulsion. The ability of fluorescent GPs to label immune cells in vivo and report on their recruitment in inflamed sites has been successfully demonstrated in a mouse model of rheumatoid arthritis (CIA) by NIRF imaging. Besides providing the visualization of the mononuclear cell infiltration in the lesion, the fluorescent signal well correlated with the clinical score associated with the disease. Very interestingly, the signal detected in lesions with the same clinical score allowed the assessment of the time evolution (progression or remission) of the pathology.
Fil: Garello, Francesca. Universitã â  Di Torino; Italia
Fil: Arena, Francesca. Universitã â  Di Torino; Italia
Fil: Cutrin, Juan Carlos. Universitã â  Di Torino; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina
Fil: Esposito, Giovanna. Universita di Torino; Italia
Fil: D'angeli, Luca. University Of London; Reino Unido
Fil: Cesano, Federico. Universita di Torino; Italia
Fil: Filippi, Miriam. Universitã â  Di Torino; Italia
Fil: Figueiredo, Sara. Universitã â  Di Torino; Italia. Universidad de Coimbra; Portugal
Fil: Terreno, Enzo. Universita di Torino; Italia
description Glucan Particles (GPs) are hollow pseudo-microspheres (average diameter 3?5 mm) obtained from common baker´s yeast Saccharomyces cerevisiae, in which mannan, lipids and proteins are removed through a process of chemical extraction. GPs shell is mostly made of 1,3-b-D-glucan that allows for their prompt in vivo uptake by immune system cells. In this work, the inner cavity of the particles has been loaded with two amphiphilic fluorescent dyes (based on cyanine for in vivo imaging purposes and rhodamine for ex vivo microscopy experiments) through a sudden change in solvent polarity that allowed the entrapment of the molecules as microemulsion. The ability of fluorescent GPs to label immune cells in vivo and report on their recruitment in inflamed sites has been successfully demonstrated in a mouse model of rheumatoid arthritis (CIA) by NIRF imaging. Besides providing the visualization of the mononuclear cell infiltration in the lesion, the fluorescent signal well correlated with the clinical score associated with the disease. Very interestingly, the signal detected in lesions with the same clinical score allowed the assessment of the time evolution (progression or remission) of the pathology.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7913
Garello, Francesca; Arena, Francesca; Cutrin, Juan Carlos; Esposito, Giovanna; D'angeli, Luca; et al.; Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis; Royal Society of Chemistry; RSC Advances; 5; 43; 5-2015; 34078-34087
2046-2069
url http://hdl.handle.net/11336/7913
identifier_str_mv Garello, Francesca; Arena, Francesca; Cutrin, Juan Carlos; Esposito, Giovanna; D'angeli, Luca; et al.; Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a mouse model of rheumatoid arthritis; Royal Society of Chemistry; RSC Advances; 5; 43; 5-2015; 34078-34087
2046-2069
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2015/ra/c5ra00720h#!divAbstract
info:eu-repo/semantics/altIdentifier/doi/10.1039/C5RA00720H
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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