Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat

Autores
Barcia, Roberto Alfredo; Pena, Liliana Beatriz; Zawoznik, Myriam Sara; Benavides, Maria Patricia; Gallego, Susana Mabel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this investigation we analyzed in detail the consequences of water deficit during the first four days of wheat development, focusing on root growth as affected by eventual changes in cell cycle regulation and oxidative processes. Root elongation decreased under water restriction in correlation with the intensity of this limitation, but the total number of cells between the quiescent center and the start of the rapid elongation zone in the root apex did not vary. Neither lipid peroxidation nor protein carbonylation increased in the roots of water-starved seedlings (ψw: -0.6 MPa); accordingly, catalase activity increased, and transcript levels of cat2 gene were enhanced. Superoxide dismutase activity rose at day 2 and 3 and, unlike catalase, displayed quite similar levels on comparing roots and coleoptiles. Proline and total soluble carbohydrates increased in the roots of water-starved seedling. Total conductivity and osmolality were also augmented. No changes in the transcript levels of the markers related to G1-S transition phase of cell cycle could be detected. However, two expansin genes (TaEXPB8 and TaEXPA5) were up-regulated in roots under water deficit. We conclude that wheat root elongation in water-deprived seedlings was simply hampered by lack of water income to cells. The enhanced expression of two root expansin genes is probably related to the eventual need of a quick cell wall expansion to allow the existing root cells to recover normal turgor, in case of sudden rewatering.
Fil: Barcia, Roberto Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Pena, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Zawoznik, Myriam Sara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Benavides, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Materia
Root Growth
Triticum Aestivum
Water Deficit
Root Apical Meristem
Oxidative Stress
Osmolyte Accumulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30837
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/30837
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheatBarcia, Roberto AlfredoPena, Liliana BeatrizZawoznik, Myriam SaraBenavides, Maria PatriciaGallego, Susana MabelRoot GrowthTriticum AestivumWater DeficitRoot Apical MeristemOxidative StressOsmolyte Accumulationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this investigation we analyzed in detail the consequences of water deficit during the first four days of wheat development, focusing on root growth as affected by eventual changes in cell cycle regulation and oxidative processes. Root elongation decreased under water restriction in correlation with the intensity of this limitation, but the total number of cells between the quiescent center and the start of the rapid elongation zone in the root apex did not vary. Neither lipid peroxidation nor protein carbonylation increased in the roots of water-starved seedlings (ψw: -0.6 MPa); accordingly, catalase activity increased, and transcript levels of cat2 gene were enhanced. Superoxide dismutase activity rose at day 2 and 3 and, unlike catalase, displayed quite similar levels on comparing roots and coleoptiles. Proline and total soluble carbohydrates increased in the roots of water-starved seedling. Total conductivity and osmolality were also augmented. No changes in the transcript levels of the markers related to G1-S transition phase of cell cycle could be detected. However, two expansin genes (TaEXPB8 and TaEXPA5) were up-regulated in roots under water deficit. We conclude that wheat root elongation in water-deprived seedlings was simply hampered by lack of water income to cells. The enhanced expression of two root expansin genes is probably related to the eventual need of a quick cell wall expansion to allow the existing root cells to recover normal turgor, in case of sudden rewatering.Fil: Barcia, Roberto Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; ArgentinaFil: Pena, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; ArgentinaFil: Zawoznik, Myriam Sara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; ArgentinaFil: Benavides, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; ArgentinaFil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; ArgentinaSpringer2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30837Gallego, Susana Mabel; Benavides, Maria Patricia; Zawoznik, Myriam Sara; Pena, Liliana Beatriz; Barcia, Roberto Alfredo; Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat; Springer; Plant Growth Regulation; 74; 2; 11-2014; 107-1170167-69031573-5087CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10725-014-9902-3info:eu-repo/semantics/altIdentifier/doi/10.1007/s10725-014-9902-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:48:19Zoai:ri.conicet.gov.ar:11336/30837instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:48:19.837CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat
title Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat
spellingShingle Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat
Barcia, Roberto Alfredo
Root Growth
Triticum Aestivum
Water Deficit
Root Apical Meristem
Oxidative Stress
Osmolyte Accumulation
title_short Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat
title_full Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat
title_fullStr Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat
title_full_unstemmed Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat
title_sort Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat
dc.creator.none.fl_str_mv Barcia, Roberto Alfredo
Pena, Liliana Beatriz
Zawoznik, Myriam Sara
Benavides, Maria Patricia
Gallego, Susana Mabel
author Barcia, Roberto Alfredo
author_facet Barcia, Roberto Alfredo
Pena, Liliana Beatriz
Zawoznik, Myriam Sara
Benavides, Maria Patricia
Gallego, Susana Mabel
author_role author
author2 Pena, Liliana Beatriz
Zawoznik, Myriam Sara
Benavides, Maria Patricia
Gallego, Susana Mabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Root Growth
Triticum Aestivum
Water Deficit
Root Apical Meristem
Oxidative Stress
Osmolyte Accumulation
topic Root Growth
Triticum Aestivum
Water Deficit
Root Apical Meristem
Oxidative Stress
Osmolyte Accumulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this investigation we analyzed in detail the consequences of water deficit during the first four days of wheat development, focusing on root growth as affected by eventual changes in cell cycle regulation and oxidative processes. Root elongation decreased under water restriction in correlation with the intensity of this limitation, but the total number of cells between the quiescent center and the start of the rapid elongation zone in the root apex did not vary. Neither lipid peroxidation nor protein carbonylation increased in the roots of water-starved seedlings (ψw: -0.6 MPa); accordingly, catalase activity increased, and transcript levels of cat2 gene were enhanced. Superoxide dismutase activity rose at day 2 and 3 and, unlike catalase, displayed quite similar levels on comparing roots and coleoptiles. Proline and total soluble carbohydrates increased in the roots of water-starved seedling. Total conductivity and osmolality were also augmented. No changes in the transcript levels of the markers related to G1-S transition phase of cell cycle could be detected. However, two expansin genes (TaEXPB8 and TaEXPA5) were up-regulated in roots under water deficit. We conclude that wheat root elongation in water-deprived seedlings was simply hampered by lack of water income to cells. The enhanced expression of two root expansin genes is probably related to the eventual need of a quick cell wall expansion to allow the existing root cells to recover normal turgor, in case of sudden rewatering.
Fil: Barcia, Roberto Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Pena, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Zawoznik, Myriam Sara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
Fil: Benavides, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
description In this investigation we analyzed in detail the consequences of water deficit during the first four days of wheat development, focusing on root growth as affected by eventual changes in cell cycle regulation and oxidative processes. Root elongation decreased under water restriction in correlation with the intensity of this limitation, but the total number of cells between the quiescent center and the start of the rapid elongation zone in the root apex did not vary. Neither lipid peroxidation nor protein carbonylation increased in the roots of water-starved seedlings (ψw: -0.6 MPa); accordingly, catalase activity increased, and transcript levels of cat2 gene were enhanced. Superoxide dismutase activity rose at day 2 and 3 and, unlike catalase, displayed quite similar levels on comparing roots and coleoptiles. Proline and total soluble carbohydrates increased in the roots of water-starved seedling. Total conductivity and osmolality were also augmented. No changes in the transcript levels of the markers related to G1-S transition phase of cell cycle could be detected. However, two expansin genes (TaEXPB8 and TaEXPA5) were up-regulated in roots under water deficit. We conclude that wheat root elongation in water-deprived seedlings was simply hampered by lack of water income to cells. The enhanced expression of two root expansin genes is probably related to the eventual need of a quick cell wall expansion to allow the existing root cells to recover normal turgor, in case of sudden rewatering.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30837
Gallego, Susana Mabel; Benavides, Maria Patricia; Zawoznik, Myriam Sara; Pena, Liliana Beatriz; Barcia, Roberto Alfredo; Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat; Springer; Plant Growth Regulation; 74; 2; 11-2014; 107-117
0167-6903
1573-5087
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30837
identifier_str_mv Gallego, Susana Mabel; Benavides, Maria Patricia; Zawoznik, Myriam Sara; Pena, Liliana Beatriz; Barcia, Roberto Alfredo; Osmotic adjustment and maintenance of the redox balance in root tissue may be key points to overcome a mild water deficit during the early growth of wheat; Springer; Plant Growth Regulation; 74; 2; 11-2014; 107-117
0167-6903
1573-5087
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10725-014-9902-3
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10725-014-9902-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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