Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots

Autores
González García, Mary Paz; Vilarrasa Blasi, Josep; Zhiponova, Miroslava; Divol, Fanchon; Mora Garcia, Santiago; Russinova, Eugenia; Caño Delgado, Ana I
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brassinosteroids (BRs) play crucial roles in plant growth and development. Previous studies have shown that BRs promote cell elongation in vegetative organs in several plant species, but their contribution to meristem homeostasis remains unexplored. Our analyses report that both loss- and gain-of-function BR-related mutants in Arabidopsis thaliana have reduced meristem size, indicating that balanced BR signalling is needed for the optimal root growth. In the BR-insensitive bri1-116 mutant, the expression pattern of the cell division markers CYCB1;1, ICK2/KRP2 and KNOLLE revealed that a decreased mitotic activity accounts for the reduced meristem size; accordingly, this defect could be overcome by the overexpression of CYCD3;1. The activity of the quiescent centre (QC) was low in the short roots of bri1-116, as reported by cell type-specific markers and differentiation phenotypes of distal stem cells. Conversely, plants treated with the most active BR, brassinolide, or mutants with enhanced BR signalling, such as bes1-D, show a premature cell cycle exit that results in early differentiation of meristematic cells, which also negatively influence meristem size and overall root growth. In the stem cell niche, BRs promote the QC renewal and differentiation of distal stem cells. Together, our results provide evidence that BRs play a regulatory role in the control of cell-cycle progression and differentiation in the Arabidopsis root meristem.
Fil: González García, Mary Paz. Centre for Research in Agricultural Genomics. Molecular Genetics Department; España
Fil: Vilarrasa Blasi, Josep. Centre for Research in Agricultural Genomics. Molecular Genetics Department; España
Fil: Zhiponova, Miroslava. Ghent University. Department of Plant Biotechnology and Genetics; Bélgica. Vlaams Instituut voor Biotechnologie; Bélgica
Fil: Divol, Fanchon. Centre for Research in Agricultural Genomics. Molecular Genetics Department; España
Fil: Mora Garcia, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina. Centre for Research in Agricultural Genomics. Molecular Genetics Department; España
Fil: Russinova, Eugenia. Ghent University. Department of Plant Biotechnology and Genetics; Bélgica. Vlaams Instituut voor Biotechnologie; Bélgica
Fil: Caño Delgado, Ana I. Centre for Research in Agricultural Genomics. Molecular Genetics Department; España
Materia
Brassinosteroids
Root
Meristem
Cell division
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12806
Acceder
id CONICETDig_80d10aa7e110aa5a7eb6235ba7d90163
oai_identifier_str oai:ri.conicet.gov.ar:11336/12806
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis rootsGonzález García, Mary PazVilarrasa Blasi, JosepZhiponova, MiroslavaDivol, FanchonMora Garcia, SantiagoRussinova, EugeniaCaño Delgado, Ana IBrassinosteroidsRootMeristemCell divisionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Brassinosteroids (BRs) play crucial roles in plant growth and development. Previous studies have shown that BRs promote cell elongation in vegetative organs in several plant species, but their contribution to meristem homeostasis remains unexplored. Our analyses report that both loss- and gain-of-function BR-related mutants in Arabidopsis thaliana have reduced meristem size, indicating that balanced BR signalling is needed for the optimal root growth. In the BR-insensitive bri1-116 mutant, the expression pattern of the cell division markers CYCB1;1, ICK2/KRP2 and KNOLLE revealed that a decreased mitotic activity accounts for the reduced meristem size; accordingly, this defect could be overcome by the overexpression of CYCD3;1. The activity of the quiescent centre (QC) was low in the short roots of bri1-116, as reported by cell type-specific markers and differentiation phenotypes of distal stem cells. Conversely, plants treated with the most active BR, brassinolide, or mutants with enhanced BR signalling, such as bes1-D, show a premature cell cycle exit that results in early differentiation of meristematic cells, which also negatively influence meristem size and overall root growth. In the stem cell niche, BRs promote the QC renewal and differentiation of distal stem cells. Together, our results provide evidence that BRs play a regulatory role in the control of cell-cycle progression and differentiation in the Arabidopsis root meristem.Fil: González García, Mary Paz. Centre for Research in Agricultural Genomics. Molecular Genetics Department; EspañaFil: Vilarrasa Blasi, Josep. Centre for Research in Agricultural Genomics. Molecular Genetics Department; EspañaFil: Zhiponova, Miroslava. Ghent University. Department of Plant Biotechnology and Genetics; Bélgica. Vlaams Instituut voor Biotechnologie; BélgicaFil: Divol, Fanchon. Centre for Research in Agricultural Genomics. Molecular Genetics Department; EspañaFil: Mora Garcia, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina. Centre for Research in Agricultural Genomics. Molecular Genetics Department; EspañaFil: Russinova, Eugenia. Ghent University. Department of Plant Biotechnology and Genetics; Bélgica. Vlaams Instituut voor Biotechnologie; BélgicaFil: Caño Delgado, Ana I. Centre for Research in Agricultural Genomics. Molecular Genetics Department; EspañaCompany Of Biologists2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12806González García, Mary Paz; Vilarrasa Blasi, Josep; Zhiponova, Miroslava; Divol, Fanchon; Mora Garcia, Santiago; et al.; Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots; Company Of Biologists; Development; 138; 5; -1-2011; 849-8590950-1991enginfo:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/content/138/5/849.longinfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1242/dev.057331info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:17:16Zoai:ri.conicet.gov.ar:11336/12806instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:17:16.982CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots
title Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots
spellingShingle Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots
González García, Mary Paz
Brassinosteroids
Root
Meristem
Cell division
title_short Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots
title_full Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots
title_fullStr Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots
title_full_unstemmed Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots
title_sort Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots
dc.creator.none.fl_str_mv González García, Mary Paz
Vilarrasa Blasi, Josep
Zhiponova, Miroslava
Divol, Fanchon
Mora Garcia, Santiago
Russinova, Eugenia
Caño Delgado, Ana I
author González García, Mary Paz
author_facet González García, Mary Paz
Vilarrasa Blasi, Josep
Zhiponova, Miroslava
Divol, Fanchon
Mora Garcia, Santiago
Russinova, Eugenia
Caño Delgado, Ana I
author_role author
author2 Vilarrasa Blasi, Josep
Zhiponova, Miroslava
Divol, Fanchon
Mora Garcia, Santiago
Russinova, Eugenia
Caño Delgado, Ana I
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Brassinosteroids
Root
Meristem
Cell division
topic Brassinosteroids
Root
Meristem
Cell division
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Brassinosteroids (BRs) play crucial roles in plant growth and development. Previous studies have shown that BRs promote cell elongation in vegetative organs in several plant species, but their contribution to meristem homeostasis remains unexplored. Our analyses report that both loss- and gain-of-function BR-related mutants in Arabidopsis thaliana have reduced meristem size, indicating that balanced BR signalling is needed for the optimal root growth. In the BR-insensitive bri1-116 mutant, the expression pattern of the cell division markers CYCB1;1, ICK2/KRP2 and KNOLLE revealed that a decreased mitotic activity accounts for the reduced meristem size; accordingly, this defect could be overcome by the overexpression of CYCD3;1. The activity of the quiescent centre (QC) was low in the short roots of bri1-116, as reported by cell type-specific markers and differentiation phenotypes of distal stem cells. Conversely, plants treated with the most active BR, brassinolide, or mutants with enhanced BR signalling, such as bes1-D, show a premature cell cycle exit that results in early differentiation of meristematic cells, which also negatively influence meristem size and overall root growth. In the stem cell niche, BRs promote the QC renewal and differentiation of distal stem cells. Together, our results provide evidence that BRs play a regulatory role in the control of cell-cycle progression and differentiation in the Arabidopsis root meristem.
Fil: González García, Mary Paz. Centre for Research in Agricultural Genomics. Molecular Genetics Department; España
Fil: Vilarrasa Blasi, Josep. Centre for Research in Agricultural Genomics. Molecular Genetics Department; España
Fil: Zhiponova, Miroslava. Ghent University. Department of Plant Biotechnology and Genetics; Bélgica. Vlaams Instituut voor Biotechnologie; Bélgica
Fil: Divol, Fanchon. Centre for Research in Agricultural Genomics. Molecular Genetics Department; España
Fil: Mora Garcia, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina. Centre for Research in Agricultural Genomics. Molecular Genetics Department; España
Fil: Russinova, Eugenia. Ghent University. Department of Plant Biotechnology and Genetics; Bélgica. Vlaams Instituut voor Biotechnologie; Bélgica
Fil: Caño Delgado, Ana I. Centre for Research in Agricultural Genomics. Molecular Genetics Department; España
description Brassinosteroids (BRs) play crucial roles in plant growth and development. Previous studies have shown that BRs promote cell elongation in vegetative organs in several plant species, but their contribution to meristem homeostasis remains unexplored. Our analyses report that both loss- and gain-of-function BR-related mutants in Arabidopsis thaliana have reduced meristem size, indicating that balanced BR signalling is needed for the optimal root growth. In the BR-insensitive bri1-116 mutant, the expression pattern of the cell division markers CYCB1;1, ICK2/KRP2 and KNOLLE revealed that a decreased mitotic activity accounts for the reduced meristem size; accordingly, this defect could be overcome by the overexpression of CYCD3;1. The activity of the quiescent centre (QC) was low in the short roots of bri1-116, as reported by cell type-specific markers and differentiation phenotypes of distal stem cells. Conversely, plants treated with the most active BR, brassinolide, or mutants with enhanced BR signalling, such as bes1-D, show a premature cell cycle exit that results in early differentiation of meristematic cells, which also negatively influence meristem size and overall root growth. In the stem cell niche, BRs promote the QC renewal and differentiation of distal stem cells. Together, our results provide evidence that BRs play a regulatory role in the control of cell-cycle progression and differentiation in the Arabidopsis root meristem.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12806
González García, Mary Paz; Vilarrasa Blasi, Josep; Zhiponova, Miroslava; Divol, Fanchon; Mora Garcia, Santiago; et al.; Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots; Company Of Biologists; Development; 138; 5; -1-2011; 849-859
0950-1991
url http://hdl.handle.net/11336/12806
identifier_str_mv González García, Mary Paz; Vilarrasa Blasi, Josep; Zhiponova, Miroslava; Divol, Fanchon; Mora Garcia, Santiago; et al.; Brassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots; Company Of Biologists; Development; 138; 5; -1-2011; 849-859
0950-1991
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dev.biologists.org/content/138/5/849.long
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1242/dev.057331
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company Of Biologists
publisher.none.fl_str_mv Company Of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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