Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney

Autores
Fridman, Osvaldo; Morales, Analía Verónica; Bortoni, Laura Egle; Turk Noceto, Paula Cecilia; Prieto, Elio A.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Betaine-homocysteine S-methyltransferase (BHMT) is an enzyme that converts homocysteine (Hcy) to methionine using betaine as a methyl donor. Betaine also acts as osmolyte in kidney medulla, protecting cells from high extracellular osmolarity. Hepatic BHMT expression is regulated by salt intake. Hormones, particularly corticosteroids, also regulate BHMT expression in rat liver. We investigated to know whether the corticoadrenal activity plays a role in kidney BHMT expression. BHMT activity in rat kidneys is several orders of magnitude lower than in rat livers and only restricted to the renal cortex. This study confirms that corticosteroids stimulate BHMT activity in the liver and, for the first time in an animal model, also up-regulate the BHMT gene expression. Besides, unlike the liver, corticosteroids in rat kidney down-regulate BHMT expression and activity. Given that the classical effect of adrenocortical activity on the kidney is associated with sodium and water re-absorption by the distal tubule leading to volume expansion, by promoting lesser use of betaine as a methyl donor, corticosteroids would preserve betaine for its other role as osmoprotectant against changes in the extracellular osmotic conditions. We conclude that corticosteroids are, at least in part, responsible for the inhibition of BHMT expression and activity in rat kidneys.
Fil: Fridman, Osvaldo. Universidad Abierta Interamericana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina
Fil: Morales, Analía Verónica. Universidad Abierta Interamericana; Argentina
Fil: Bortoni, Laura Egle. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina
Fil: Turk Noceto, Paula Cecilia. Universidad Abierta Interamericana; Argentina
Fil: Prieto, Elio A.. Universidad Abierta Interamericana; Argentina
Materia
BETAINE
HOMOCYSTEINE
METHYLATION
OSMOLYTE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199039
Acceder
id CONICETDig_5d47762d614a0604d38cfd432ac2adc5
oai_identifier_str oai:ri.conicet.gov.ar:11336/199039
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidneyFridman, OsvaldoMorales, Analía VerónicaBortoni, Laura EgleTurk Noceto, Paula CeciliaPrieto, Elio A.BETAINEHOMOCYSTEINEMETHYLATIONOSMOLYTEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Betaine-homocysteine S-methyltransferase (BHMT) is an enzyme that converts homocysteine (Hcy) to methionine using betaine as a methyl donor. Betaine also acts as osmolyte in kidney medulla, protecting cells from high extracellular osmolarity. Hepatic BHMT expression is regulated by salt intake. Hormones, particularly corticosteroids, also regulate BHMT expression in rat liver. We investigated to know whether the corticoadrenal activity plays a role in kidney BHMT expression. BHMT activity in rat kidneys is several orders of magnitude lower than in rat livers and only restricted to the renal cortex. This study confirms that corticosteroids stimulate BHMT activity in the liver and, for the first time in an animal model, also up-regulate the BHMT gene expression. Besides, unlike the liver, corticosteroids in rat kidney down-regulate BHMT expression and activity. Given that the classical effect of adrenocortical activity on the kidney is associated with sodium and water re-absorption by the distal tubule leading to volume expansion, by promoting lesser use of betaine as a methyl donor, corticosteroids would preserve betaine for its other role as osmoprotectant against changes in the extracellular osmotic conditions. We conclude that corticosteroids are, at least in part, responsible for the inhibition of BHMT expression and activity in rat kidneys.Fil: Fridman, Osvaldo. Universidad Abierta Interamericana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; ArgentinaFil: Morales, Analía Verónica. Universidad Abierta Interamericana; ArgentinaFil: Bortoni, Laura Egle. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; ArgentinaFil: Turk Noceto, Paula Cecilia. Universidad Abierta Interamericana; ArgentinaFil: Prieto, Elio A.. Universidad Abierta Interamericana; ArgentinaIndian Academy of Sciences2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199039Fridman, Osvaldo; Morales, Analía Verónica; Bortoni, Laura Egle; Turk Noceto, Paula Cecilia; Prieto, Elio A.; Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney; Indian Academy of Sciences; Journal of Biosciences; 37; 1; 12-2012; 115-1230250-5991CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s12038-011-9171-5info:eu-repo/semantics/altIdentifier/doi/10.1007/s12038-011-9171-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:12Zoai:ri.conicet.gov.ar:11336/199039instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:12.446CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney
title Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney
spellingShingle Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney
Fridman, Osvaldo
BETAINE
HOMOCYSTEINE
METHYLATION
OSMOLYTE
title_short Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney
title_full Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney
title_fullStr Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney
title_full_unstemmed Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney
title_sort Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney
dc.creator.none.fl_str_mv Fridman, Osvaldo
Morales, Analía Verónica
Bortoni, Laura Egle
Turk Noceto, Paula Cecilia
Prieto, Elio A.
author Fridman, Osvaldo
author_facet Fridman, Osvaldo
Morales, Analía Verónica
Bortoni, Laura Egle
Turk Noceto, Paula Cecilia
Prieto, Elio A.
author_role author
author2 Morales, Analía Verónica
Bortoni, Laura Egle
Turk Noceto, Paula Cecilia
Prieto, Elio A.
author2_role author
author
author
author
dc.subject.none.fl_str_mv BETAINE
HOMOCYSTEINE
METHYLATION
OSMOLYTE
topic BETAINE
HOMOCYSTEINE
METHYLATION
OSMOLYTE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Betaine-homocysteine S-methyltransferase (BHMT) is an enzyme that converts homocysteine (Hcy) to methionine using betaine as a methyl donor. Betaine also acts as osmolyte in kidney medulla, protecting cells from high extracellular osmolarity. Hepatic BHMT expression is regulated by salt intake. Hormones, particularly corticosteroids, also regulate BHMT expression in rat liver. We investigated to know whether the corticoadrenal activity plays a role in kidney BHMT expression. BHMT activity in rat kidneys is several orders of magnitude lower than in rat livers and only restricted to the renal cortex. This study confirms that corticosteroids stimulate BHMT activity in the liver and, for the first time in an animal model, also up-regulate the BHMT gene expression. Besides, unlike the liver, corticosteroids in rat kidney down-regulate BHMT expression and activity. Given that the classical effect of adrenocortical activity on the kidney is associated with sodium and water re-absorption by the distal tubule leading to volume expansion, by promoting lesser use of betaine as a methyl donor, corticosteroids would preserve betaine for its other role as osmoprotectant against changes in the extracellular osmotic conditions. We conclude that corticosteroids are, at least in part, responsible for the inhibition of BHMT expression and activity in rat kidneys.
Fil: Fridman, Osvaldo. Universidad Abierta Interamericana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina
Fil: Morales, Analía Verónica. Universidad Abierta Interamericana; Argentina
Fil: Bortoni, Laura Egle. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina
Fil: Turk Noceto, Paula Cecilia. Universidad Abierta Interamericana; Argentina
Fil: Prieto, Elio A.. Universidad Abierta Interamericana; Argentina
description Betaine-homocysteine S-methyltransferase (BHMT) is an enzyme that converts homocysteine (Hcy) to methionine using betaine as a methyl donor. Betaine also acts as osmolyte in kidney medulla, protecting cells from high extracellular osmolarity. Hepatic BHMT expression is regulated by salt intake. Hormones, particularly corticosteroids, also regulate BHMT expression in rat liver. We investigated to know whether the corticoadrenal activity plays a role in kidney BHMT expression. BHMT activity in rat kidneys is several orders of magnitude lower than in rat livers and only restricted to the renal cortex. This study confirms that corticosteroids stimulate BHMT activity in the liver and, for the first time in an animal model, also up-regulate the BHMT gene expression. Besides, unlike the liver, corticosteroids in rat kidney down-regulate BHMT expression and activity. Given that the classical effect of adrenocortical activity on the kidney is associated with sodium and water re-absorption by the distal tubule leading to volume expansion, by promoting lesser use of betaine as a methyl donor, corticosteroids would preserve betaine for its other role as osmoprotectant against changes in the extracellular osmotic conditions. We conclude that corticosteroids are, at least in part, responsible for the inhibition of BHMT expression and activity in rat kidneys.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199039
Fridman, Osvaldo; Morales, Analía Verónica; Bortoni, Laura Egle; Turk Noceto, Paula Cecilia; Prieto, Elio A.; Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney; Indian Academy of Sciences; Journal of Biosciences; 37; 1; 12-2012; 115-123
0250-5991
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199039
identifier_str_mv Fridman, Osvaldo; Morales, Analía Verónica; Bortoni, Laura Egle; Turk Noceto, Paula Cecilia; Prieto, Elio A.; Corticoadrenal activity regulates in rat betaine-homocysteine S-methyltransferase expression with opposites effects in liver and kidney; Indian Academy of Sciences; Journal of Biosciences; 37; 1; 12-2012; 115-123
0250-5991
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s12038-011-9171-5
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12038-011-9171-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Indian Academy of Sciences
publisher.none.fl_str_mv Indian Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270110758207488
score 13.13397

Publicaciones similares