Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury

Autores
Boero, Luis Ezequiel; Goutman, Juan Diego; Elgoyhen, Ana Belen; Gomez Casati, Maria Eugenia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Noise induced hearing loss (NIHL) has become a major public health problem. In order to address the role of the efferent olivocochlear system in NIHL we made use of a mouse model in which the alpha9 nicotinic receptor subunit bears a mutation and leads to enhanced medial efferent activity (Chrna9L9T knock-in (KI)) in addition to one lacking the alpha9 subunit of the nicotinic receptor (Chrna9 knockout (KO)).We exposed WT, Chrna9L9T KI and Chrna9 KO mice to loud sounds (1-16 kHz, 100 dB SPL, 1hr) and measured auditory brainstem responses (ABR), which reflect synchronized discharges from neurons along the auditory pathway. We tested outer hair cell function by recording the distortion product otoacoustic emissions (DPOAEs). Large auditory threshold shifts were found one day after exposure in WT and Chrna9 KO mice. However, one week later, thresholds returned to normal in WT, whereas the Chrna9 KO ears did not recover. In contrast, Chrna9L9T KI mice were resistant to the same noise exposure. Finally, we used immunohistochemistry to visualize efferent neurons and found a reduction in the number of terminals after trauma in WT mice. Immunofluorescence against Ctbp2, a protein located in inner hair cell (IHC) ribbon synapse, revealed a decrease in the number of ribbon synapses per IHC after acoustic trauma in WT mice. These findings suggest a key role of alpha9alpha10 nAChRs in the efferent-mediated noise protection.
Fil: Boero, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Gomez Casati, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXX Annual Meeting and SAN-ISN Small Conference and Course
Mar del Plata
Argentina
Sociedad Argentina de Investigaciones en Neurociencias
Materia
EFFERENT SYSTEM
ACOUSTIC TRAUMA
COCHLEAR HAIR CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215098
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/215098
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injuryBoero, Luis EzequielGoutman, Juan DiegoElgoyhen, Ana BelenGomez Casati, Maria EugeniaEFFERENT SYSTEMACOUSTIC TRAUMACOCHLEAR HAIR CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Noise induced hearing loss (NIHL) has become a major public health problem. In order to address the role of the efferent olivocochlear system in NIHL we made use of a mouse model in which the alpha9 nicotinic receptor subunit bears a mutation and leads to enhanced medial efferent activity (Chrna9L9T knock-in (KI)) in addition to one lacking the alpha9 subunit of the nicotinic receptor (Chrna9 knockout (KO)).We exposed WT, Chrna9L9T KI and Chrna9 KO mice to loud sounds (1-16 kHz, 100 dB SPL, 1hr) and measured auditory brainstem responses (ABR), which reflect synchronized discharges from neurons along the auditory pathway. We tested outer hair cell function by recording the distortion product otoacoustic emissions (DPOAEs). Large auditory threshold shifts were found one day after exposure in WT and Chrna9 KO mice. However, one week later, thresholds returned to normal in WT, whereas the Chrna9 KO ears did not recover. In contrast, Chrna9L9T KI mice were resistant to the same noise exposure. Finally, we used immunohistochemistry to visualize efferent neurons and found a reduction in the number of terminals after trauma in WT mice. Immunofluorescence against Ctbp2, a protein located in inner hair cell (IHC) ribbon synapse, revealed a decrease in the number of ribbon synapses per IHC after acoustic trauma in WT mice. These findings suggest a key role of alpha9alpha10 nAChRs in the efferent-mediated noise protection.Fil: Boero, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Gomez Casati, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaXXX Annual Meeting and SAN-ISN Small Conference and CourseMar del PlataArgentinaSociedad Argentina de Investigaciones en NeurocienciasSociedad Argentina de Investigación en Neurociencias2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215098Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury; XXX Annual Meeting and SAN-ISN Small Conference and Course; Mar del Plata; Argentina; 2015; 322-322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2015_Mar-del-Plata._libro-de-resumenes.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:02:11Zoai:ri.conicet.gov.ar:11336/215098instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:02:12.0CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury
title Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury
spellingShingle Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury
Boero, Luis Ezequiel
EFFERENT SYSTEM
ACOUSTIC TRAUMA
COCHLEAR HAIR CELLS
title_short Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury
title_full Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury
title_fullStr Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury
title_full_unstemmed Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury
title_sort Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury
dc.creator.none.fl_str_mv Boero, Luis Ezequiel
Goutman, Juan Diego
Elgoyhen, Ana Belen
Gomez Casati, Maria Eugenia
author Boero, Luis Ezequiel
author_facet Boero, Luis Ezequiel
Goutman, Juan Diego
Elgoyhen, Ana Belen
Gomez Casati, Maria Eugenia
author_role author
author2 Goutman, Juan Diego
Elgoyhen, Ana Belen
Gomez Casati, Maria Eugenia
author2_role author
author
author
dc.subject.none.fl_str_mv EFFERENT SYSTEM
ACOUSTIC TRAUMA
COCHLEAR HAIR CELLS
topic EFFERENT SYSTEM
ACOUSTIC TRAUMA
COCHLEAR HAIR CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Noise induced hearing loss (NIHL) has become a major public health problem. In order to address the role of the efferent olivocochlear system in NIHL we made use of a mouse model in which the alpha9 nicotinic receptor subunit bears a mutation and leads to enhanced medial efferent activity (Chrna9L9T knock-in (KI)) in addition to one lacking the alpha9 subunit of the nicotinic receptor (Chrna9 knockout (KO)).We exposed WT, Chrna9L9T KI and Chrna9 KO mice to loud sounds (1-16 kHz, 100 dB SPL, 1hr) and measured auditory brainstem responses (ABR), which reflect synchronized discharges from neurons along the auditory pathway. We tested outer hair cell function by recording the distortion product otoacoustic emissions (DPOAEs). Large auditory threshold shifts were found one day after exposure in WT and Chrna9 KO mice. However, one week later, thresholds returned to normal in WT, whereas the Chrna9 KO ears did not recover. In contrast, Chrna9L9T KI mice were resistant to the same noise exposure. Finally, we used immunohistochemistry to visualize efferent neurons and found a reduction in the number of terminals after trauma in WT mice. Immunofluorescence against Ctbp2, a protein located in inner hair cell (IHC) ribbon synapse, revealed a decrease in the number of ribbon synapses per IHC after acoustic trauma in WT mice. These findings suggest a key role of alpha9alpha10 nAChRs in the efferent-mediated noise protection.
Fil: Boero, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Gomez Casati, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXX Annual Meeting and SAN-ISN Small Conference and Course
Mar del Plata
Argentina
Sociedad Argentina de Investigaciones en Neurociencias
description Noise induced hearing loss (NIHL) has become a major public health problem. In order to address the role of the efferent olivocochlear system in NIHL we made use of a mouse model in which the alpha9 nicotinic receptor subunit bears a mutation and leads to enhanced medial efferent activity (Chrna9L9T knock-in (KI)) in addition to one lacking the alpha9 subunit of the nicotinic receptor (Chrna9 knockout (KO)).We exposed WT, Chrna9L9T KI and Chrna9 KO mice to loud sounds (1-16 kHz, 100 dB SPL, 1hr) and measured auditory brainstem responses (ABR), which reflect synchronized discharges from neurons along the auditory pathway. We tested outer hair cell function by recording the distortion product otoacoustic emissions (DPOAEs). Large auditory threshold shifts were found one day after exposure in WT and Chrna9 KO mice. However, one week later, thresholds returned to normal in WT, whereas the Chrna9 KO ears did not recover. In contrast, Chrna9L9T KI mice were resistant to the same noise exposure. Finally, we used immunohistochemistry to visualize efferent neurons and found a reduction in the number of terminals after trauma in WT mice. Immunofluorescence against Ctbp2, a protein located in inner hair cell (IHC) ribbon synapse, revealed a decrease in the number of ribbon synapses per IHC after acoustic trauma in WT mice. These findings suggest a key role of alpha9alpha10 nAChRs in the efferent-mediated noise protection.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215098
Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury; XXX Annual Meeting and SAN-ISN Small Conference and Course; Mar del Plata; Argentina; 2015; 322-322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215098
identifier_str_mv Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury; XXX Annual Meeting and SAN-ISN Small Conference and Course; Mar del Plata; Argentina; 2015; 322-322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2015_Mar-del-Plata._libro-de-resumenes.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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